Download or read book Hybrid Solid state Nanopores for Stochastic Single molecule Detection written by and published by . This book was released on 2015 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Single Molecule Detection with Hybrid Nanopores written by Oliver James Pambos and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Engineered Nanopores for Bioanalytical Applications written by Joshua B. Edel and published by William Andrew. This book was released on 2013-03-19 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: Engineered Nanopores for Bioanalytical Applications is the first book to focus primarily on practical analytical applications of nanopore development. These nanoscale analytical techniques have exciting potential because they can be used in applications such as DNA sequencing, DNA fragment sizing, DNA/protein binding, and protein/protein binding.This book provides a solid professional reference on nanopores for readers in academia, industry and engineering and biomedical fields. In addition, the book describes the instrumentation, fabrication, and experimental methods necessary to carry out nanopore-based experiments for developing new devices. - Includes application case studies for detection, identification and analysis of biomolecules and related functional nanomaterials - Introduces the techniques of manufacturing solid state materials with functional nanopores - Explains the use of nanopores in DNA sequencing and the wider range of applications from environmental monitoring to forensics
Download or read book Single Molecule Delivery and Detection Into Crowded Environment with a Solid state Nanopore written by Chi Cheng Chau and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nanopores written by Samir M. Iqbal and published by Springer Science & Business Media. This book was released on 2011-07-12 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanopores are nanometer scale holes formed naturally by proteins or cells, and can be used for a variety of applications, including sequencing DNA and detecting anthrax. They can be integrated into artificially constructed encapsulated cells of silicon wafers while allowing small molecules like oxygen, glucose and insulin to pass, while keeping out large system molecules. "Nanopores: Sensing and Fundamental Biological Interactions" examines the emerging research directions surrounding nanopores such as genome sequencing and early disease detection using biomarker identification. Covering the applications of nanopores in genetics, proteomics, drug discovery, early disease detection and detection of emerging environmental threats, it is a must-have book for biomedicalengineers and research scientists.
Download or read book Polymer Translocation written by M. Muthukumar and published by CRC Press. This book was released on 2016-04-19 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: Polymer translocation occurs in many biological and biotechnological phenomena where electrically charged polymer molecules move through narrow spaces in crowded environments. Unraveling the rich phenomenology of polymer translocation requires a grasp of modern concepts of polymer physics and polyelectrolyte behavior. Polymer Translocation discusse
Download or read book Optimizing the Single Molecule Counting Process of Solid State Nanopores written by Martin Charron and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to their intrinsic single-molecule resolution and now easy fabrication and microfluidic integration, solid-state nanopores show great potential of becoming flexible low-cost, point-of-need, ultra-sensitive biomarker detection sensors. Since nanopores are still limited by the arrival time of the analyte to the sensor, reaching ultra-low concentration levels (fM) in a reasonable measurement time remains a challenge. Before approaches solving this problem become possible, one subject, not often discussed, needs to be addressed: The reliability and uncertainty of capture-based nanopore measurements. Counting with nanopores is often accomplished through measuring translocation frequencies. However, limitations of nanofabrication techniques tend to produce solid-state nanopores with, at the atomic scale, different geometries or chemical structures. A question then arises: How different will the measured capture rate be for two seemingly identical pores, i.e. pores intended to have same diameter and thickness within the fabrication capabilities? One solution to circumvent this problem is to use calibration curves by measuring the capture frequency of different concentrations, which prompts a follow up question: Does a single nanopore capture frequency change over time during the course of an experiment? This thesis investigates these two questions and experimentally shows that intra-pore and inter-pore capture variations can be significant, thus reducing the precision of simple nanopore counting to determine concentration of an analyte. To address these complications, a solution involving an internal calibrator is presented and is demonstrated to increase the sensitivity of concentration measurements.
Download or read book Trapping Single Molecules with a Solid State Nanopore written by Marc Herman Gershow and published by . This book was released on 2008 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: I describe a system for measuring and manipulating single molecules using a solid state nanopore, a small hole in an insulating film fashioned using solid-state Silicon processing techniques. In this work, pores less than 10 nanometers in diameter in 20-30 nm thick Silicon Nitride membranes are used to join two reservoirs of salt water. DNA molecules added to one reservoir are forced through the nanopore by an applied voltage bias. Single molecules of DNA are detected passing through the pore by a blockage of the ionic current between the reservoirs. The depth and duration of the current blockages reveal information about the molecule's geometry while the shape of the blockage depends on the molecule's conformation. If the driving voltage is reversed soon after a molecule has passed through the nanopore, the same molecule can be "recaptured," induced to pass through the pore a second time. This process reveals information about the dynamics of the molecule outside of and its interaction with the nanopore. The recapture process can be repeated multiple times to form a single molecule trap.
Download or read book Cancer Genomics written by Hye-Jung E. Chun and published by Elsevier Inc. Chapters. This book was released on 2013-11-21 with total page 48 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cancer results from accumulated mutations in the genome. Sequencing is an accurate method to detect mutations. Second-generation sequencing technology, commonly referred to as next-generation sequencing technology, enables rapid, efficient and affordable DNA sequencing, and is transforming the scale and scope of cancer research. The technology is sufficiently flexible and affordable to allow sequencing of many cancer genomes, and thus facilitates both sequencing of samples from large patient cohorts and during disease progression in individual cancer patients. The high depths of redundant sequence coverage that can be obtained using some second-generation sequencing technologies, along with sequencing reads amplified from single DNA molecules, facilitate detection of subclones of cells in tumors. Large-scale genome sequencing of hundreds or even thousands of cancer samples is being conducted by several groups that aim to identify and characterize cancer driver mutations. Goals of such work, previously infeasible with Sanger sequencing instruments, are to use this information to improve cancer prognosis, diagnosis and therapeutic decision-making. The speed of data analysis is rate limiting, and investigators are struggling to accommodate and interpret the data deluge produced by second-generation technologies. In this chapter, we discuss cancer properties that are revealed by sequencing and the implication of such properties in experimental design and data interpretation. We describe past, current and upcoming sequencing technologies and the application of second-generation sequencing technologies in cancer genomics. Finally, we discuss the impact of second-generation sequencing technology in shaping personalized medicine.
Download or read book Biological and Bio inspired Nanomaterials written by Sarah Perrett and published by Springer Nature. This book was released on 2019-11-11 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes naturally occurring and designed bio-inspired molecular building blocks assembled into nanoscale structures. It covers a fascinating array of biomimetic and bioinspired materials, including inorganic nanozymes, structures formed by DNA origami, a wide range of peptide and protein-based nanomaterials, as well as their applications in diagnostics and therapeutics. The book elucidates the mechanism of assembly of these materials and characterisation of their mechanical and physico-chemical properties which inspires readers not only to exploit the potential applications of nanomaterials, but also to understand their potential risks and benefits. It will be of interest to a broad audience of students and researchers spanning the disciplines of biology, chemistry, engineering, materials science, and physics.
Download or read book Biomembrane Frontiers written by Thomas Jue and published by Springer Science & Business Media. This book was released on 2009-06-13 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the second book in the Handbook of Modern Biophysics series, dedicated to fundamental topics and new applications in biophysics. This book on biomembranes covers theory and application and includes problem sets, references and guides for further study.
Download or read book Handbook of Single Molecule Biophysics written by Peter Hinterdorfer and published by Springer Science & Business Media. This book was released on 2009-12-24 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.
Download or read book Nucleic Acid Amplification Strategies for Biosensing Bioimaging and Biomedicine written by Shusheng Zhang and published by Springer. This book was released on 2019-04-25 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the rational design, development and application of nucleic acid amplification strategies for biosensing, bioimaging and biomedicine. It consists of fifteen chapters demonstrating the use of these strategies in various areas, including fluorescence techniques, Chemiluminescence biosensors, electrochemiluminescence biosensors, colorimetric assays, surface plasmon resonance technologies, electrochemical DNA sensors, photoelectrochemical biosensor, nanopore sensors, quartz crystal microbalance, fluorescence imaging, surface-enhanced Raman spectroscopy, in vitro and in vivo metal ions detection, theranostics and microdroplet chips. Offering a collection of reviews illustrating the latest advances in biochemical analysis and therapeutics, the book shares valuable insights into current challenges and future prospects, making it a valuable resource for a wide readership in the various fields of biosensing, bioimaging and biomedicine.
Download or read book Electrophysiology of Unconventional Channels and Pores written by Anne H. Delcour and published by Springer. This book was released on 2015-10-23 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is dedicated to the channels and pores that belong to an eclectic and ubiquitous class of unconventional - perhaps at times strange - pore-forming molecules, which nevertheless play fundamental roles in various organisms. These non-canonical channels may take on various and sometimes complex architectures, such as large beta-barrels or lipid-containing pores. They may originate from bacteria, viruses or intracellular organelles. For some of them, the physiologically relevant substrate may indeed be ions, and for others folded polypeptides. Some are released by cells in a soluble form that has the ability to insert into biological membranes to exert its permeabilizing effect. Many of these unconventional pores have been investigated by electrophysiology, which, by its virtue of focusing on a few or even a single unit, has provided invaluable insight into the mechanisms and structure-function relationships of these remarkable membrane entities. The chapters of this book highlight a representative set of these interesting investigations.
Download or read book Sensing Native and Unfolded Single Protein Molecules with Solid state Nanopores written by Bradley Thomas Ledden and published by . This book was released on 2011 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cancer Genomics written by Graham Dellaire and published by Academic Press. This book was released on 2013-11-21 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cancer Genomics addresses how recent technological advances in genomics are shaping how we diagnose and treat cancer. Built on the historical context of cancer genetics over the past 30 years, the book provides a snapshot of the current issues and state-of-the-art technologies used in cancer genomics. Subsequent chapters highlight how these approaches have informed our understanding of hereditary cancer syndromes and the diagnosis, treatment and outcome in a variety of adult and pediatric solid tumors and hematologic malignancies. The dramatic increase in cancer genomics research and ever-increasing availability of genomic testing are not without significant ethical issues, which are addressed in the context of the return of research results and the legal considerations underlying the commercialization of genomic discoveries. Finally, the book concludes with "Future Directions", examining the next great challenges to face the field of cancer genomics, namely the contribution of non-coding RNAs to disease pathogenesis and the interaction of the human genome with the environment. - Tools such as sidebars, key concept summaries, a glossary, and acronym and abbreviation definitions make this book highly accessible to researchers from several fields associated with cancer genomics. - Contributions from thought leaders provide valuable historical perspective to relate the advances in the field to current technologies and literature.
Download or read book Mathematics of the Incas written by Marcia Ascher and published by Courier Corporation. This book was released on 2013-01-02 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: Unique, thought-provoking study discusses quipu, an accounting system employing knotted, colored cords, used by Incas. Cultural context, mathematics involved, and even how to make a quipu. Over 125 illustrations.
