Download or read book HDBK HPLC FOR SEPARATION AMINO ACIDS PEPTIDES PROTEINS written by William S. Hancock and published by CRC-Press. This book was released on 1984-07-02 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction to the general principles of HPLC; HPLC instrumentation; Mobile phases; Detection methods; Separation of free amino acids; Resolution of amino acids as diastereomeric derivatives.
Download or read book Handbook HPLC for Separation Amino Acids Peptides and Proteins written by William S. Hancock and published by CRC-Press. This book was released on 1984-08-14 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: All areas of high performance liquid chromatography (HPLC) relevant to the separation of amino acid and polypeptide samples are described in these comprehensive volumes. Eighty-four chapters cover the application of HPLC to research in the life sciences. Both basic information and specific details are presented on such areas as commercially available HPLC columns, ligand-exchange chromatography, peptide hormone separations, peptide antibiotic separations and drug-protein binding. Practical suggestions are given to help you avoid pitfalls which can exist in the analysis of biological materials.
Download or read book Crc Handbook of HPLC for the Separation of Amino Acids Peptides and Proteins written by Taylor & Francis Group and published by . This book was released on 2018-09-30 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book CRC Handbook of HPLC for the Separation of Amino Acids Peptides and Proteins written by Chemical Rubber Company (Cleveland, Ohio) and published by . This book was released on 1989 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book HDBK HPLC FOR SEPARATION AMINO ACIDS PEPTIDES PROTEINS written by William S. Hancock and published by CRC-Press. This book was released on 1984-07-02 with total page 489 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction to the general principles of HPLC; HPLC instrumentation; Mobile phases; Detection methods; Separation of free amino acids; Resolution of amino acids as diastereomeric derivatives.
Download or read book A Practical Guide to Protein and Peptide Purification for Microsequencing written by Paul T. Matsudaira and published by Elsevier. This book was released on 2012-12-02 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Mass spectrophotometric characterization of proteins and peptides N-terminal sequencing of proteins with blocked N termini Internal amino acid sequence analysis after protease digestion in-gel and on-blot Improved microscale peptide purification methods Computer analysis of protein sequences New protocols tested and refined through everyday use in authors' laboratories Updated reference chapter covering all aspects of protein microsequencing
Download or read book High Performance Liquid Chromatography of Peptides and Proteins written by Colin T. Mant and published by CRC Press. This book was released on 2017-11-22 with total page 960 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.
Download or read book Handbook of HPLC written by Danilo Corradini and published by CRC Press. This book was released on 2016-04-19 with total page 715 pages. Available in PDF, EPUB and Kindle. Book excerpt: High performance liquid chromatography (HPLC) is one of the most widespread analytical and preparative scale separation techniques used for both scientific investigations and industrial and biomedical analysis. Now in its second edition, this revised and updated version of the Handbook of HPLC examines the new advances made in this field since the
Download or read book HPLC of Peptides and Proteins written by Marie-Isabel Aguilar and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.
Download or read book Practical Protein Chemistry written by A. Darbre and published by . This book was released on 1986-06-03 with total page 650 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein chemistry is a wide field embracing the analysis, synthesis, sequencing and separation of peptides, proteins and individual amino acids. Practical protein chemistry is a survey of proven techniques designed primarily to be a laboratory handbook for research workers involved with proteins.
Download or read book CRC Handbook of Chromatography written by Ram N. Gupta and published by CRC Press. This book was released on 2019-01-08 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: These volumes provide a reference source of different gas chro-matographic, liquid chromatographic, or thin-layer chromatographic techniques for the qualitative determination of various therapeutic agents, including antibiotics, vitamins and hormones, drugs of abuse in body fluids, dosage forms, or food stuffs. Over 5000 publi-cations were reviewed to prepare tables of chromatographic data for 800 compounds, arranged alphabetically by generic drug name or by drug groups. A detailed summary of the extraction procedure de-scribed in each publication included in the table of a particular drug is also provided. This easy-to-read handbook is useful for se-lecting an appropriate chromatographic procedure for the determi-nation of a given compound according to the available facilities.
Download or read book Amino Acid Handbook written by Richard Joseph Block and published by . This book was released on 1956 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book CRC Handbook of Chromatography written by S. Blackburn and published by CRC Press. This book was released on 1989-05-31 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: An in-depth analysis of chromatography literature and procedures since 1981 is presented in this publication. Featured is a compre-hensive range of tables relating to the chromatographic separation and determination of amino acids, amines, and their derivatives. Methods of sample preparation and derivatization and methods of detection are described. Included are techniques for the liquid and gas chro-matographic separation of free amino acids and their derivatives, including o-phthalaldehyde, dansyl, and phenylthiolaydantoin deriva-tives. The separation of amino acid enantiomers is also described. This book will be invaluable to chemists, biochemists, and analysts involved in the separation and determination of amino acids or amines and their derivatives.
Download or read book Handbook of HPLC written by Danilo Corradini and published by CRC Press. This book was released on 2011-01-03 with total page 1018 pages. Available in PDF, EPUB and Kindle. Book excerpt: Delineating its usage in separation, purification and detection processes across a variety of disciplines, from industry to applied research, this work discusses the principles, techniques and instrumentation involving HPLC within a detailed framework. Over 100 tables present previously scattered experimental data.
Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2008-02-12 with total page 759 pages. Available in PDF, EPUB and Kindle. Book excerpt: In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.
Download or read book Food Analysis by HPLC Second Edition written by Leo M.L. Nollet and published by CRC Press. This book was released on 2000-04-05 with total page 1074 pages. Available in PDF, EPUB and Kindle. Book excerpt: Food Analysis by HPLC, Second Edition presents an exhaustive compilation of analytical methods that belong in the toolbox of every practicing food chemist. Topics covered include biosensors, BMO’s, nanoscale analysis systems, food authenticity, radionuclides concentration, meat factors and meat quality, particle size analysis, and scanning colorimity. It also analyzes peptides, carbohydrates, vitamins, and food additives and contains chapters on alcohols, phenolic compounds, pigments, and residues of growth promoters. Attuned to contemporary food industry concerns, this bestselling classic also features topical coverage of the quantification of genetically modified organisms in food.
Download or read book Methods of Protein Microcharacterization written by John E. Shively and published by Springer Science & Business Media. This book was released on 2008-02-22 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N, N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of polypeptides was automated amino acid analysis described by Spackman et al. in 1958 (6). However, during the period from 1975 to 1980, it became increasingly clear that the amount of material required for struc tural analysis was more than could be easily isolated for the vast majority of proteins. The field was criticized for its lack of sensitive techniques for the analysis of growth factors, immune modulators, membrane receptors, and peptide hormones. In addition, very little had been done to modernize and improve the original instruments introduced in the mid-1960s. The first indications of improved instrumentation for Edman chemistry came from Wittmann-Liebold's laboratory (7), followed by the introduction of a "micro" sequencer by Hunkapiller and Hood in 1978 (8). The movement toward improved instrumentation culminated in the "gas"--Phase sequencer of Hewick et al. (9) in 1981
