Download or read book Genetic Interaction Between Phaseolus Vulgaris and Bean Common Mosaic Virus with Implications for Strain Identification and Breeding for Resistance written by and published by . This book was released on 1978 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Interaction Between Phaseolus Vulgaris and Bean Common Mosaic Virus with Implications for Strain Identification and Breeding for Resistance written by E. Drijfhout and published by Bernan Press(PA). This book was released on 1978 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt: General introduction; Review of the literature and aims of this study; Materials and methods; Analysis of host reaction and identification of strains; Inheritance of resistance of plants with recessive alleles of the necrosis gene; Genotypes for resistance and pathogenicity; Inheritance of resistance of plants with dominant necrosis gene; Some implications for breeding.
Download or read book Genetic Relationship Between the Reaction of Dry Bean Genotypes Phaseolus Vulgaris L to Bean Common Mosaic Virus and Bean Yellow Mosaic Virus written by Martin Nyololani Mbewe and published by . This book was released on 1989 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Interaction Between Phaseolus Vulgeris and Bean Common Mosaic Virus with Implications for Strain Indentification written by Drijhout E. and published by . This book was released on 1978 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Gene Ecology of the Climbing Common Bean Phaseolus Vulgaris Bean Common Mosaic Virus Bean Common Mosaic Necrosis Virus BCMV BCMVN Relationship in Rwanda written by Alice Kabeja and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Common beans (Phaseolus vulgaris) are nutritionally, culturally, and economically an important legume crop in Rwanda. Beans provide 32 and 65 percent of calories and protein intake in the Rwandan diet, whereas protein sourced from animal provides only 4 percent of the protein intake. Unfortunately, common bean production is constrained by multiple biotic and abiotic factors in this country. Among others, Bean common mosaic virus and Bean common mosaic necrosis virus (BCMV/BCMNV) are seedborne viral pathogens that are the main constraint in common bean production. The only effective control of these pathogens is through the use of genetic resistance. Currently, the status of BCMV/BCMNV in Rwanda is not known. To contribute to the research in breeding common bean against BCMV/BCMNV in Rwanda, I sought to better understand the variability and biology of the BCMV/BCMNV pathogen to assist in developing a resistance strategy in common bean that is broader and stronger. In the first chapter, I was interested in determining the types of BCMV/BCMNV strains that are present in Rwanda. I was also interested in identifying the selective pressures these viruses are subjected to and how they govern the evolution of these viruses in Rwanda. Furthermore, I wanted to find out if there are alternate hosts other than common bean for BCMV/BCMNV in Rwanda. To gain this information, I surveyed common bean fields extensively in high-altitude areas of Rwanda. The biological and molecular pathogenic characterization studies of isolates of BCMV and BCMNV resulting from these surveys revealed the exclusive presence of BCMNV in Rwanda. No BCMV strain were found. We observed the predominance of the NL-3 strain of BCMNV. The study of genetic diversity for coat protein (CP) fragment of the BCMNV isolates showed a low genetic diversity ([pi] = 0.015623). The mean [omega] (dN/dS) value for CP of 27 isolates analyzed here was less than 1.0, indicating that the CP segment of BCMNV in Rwanda is subject to negative or purifying selection. No alternative hosts to Phaseolus vulgaris for either BCMV or BCMNV were identified in this study. For now, the resistance of BCMV/BCMNV in host plant is controlled by seven resistance genes: one dominant gene (I gene), five recessive strain-specific genes (bc-1, bc-12, bc-2, bc-22, and bc-3), and one strain-nonspecific epistatic gene (bc-u). However, few studies have been carried out on bc-12 (Miklas et al.; 2000). In the second chapter, through the development and evaluation of Recombinant Inbreed Lines (RIL) under greenhouse conditions, we investigated if any minor, quantitative loci could be identified for resistance to BCMNV, which would have originated in either of the parents, RWV3006 (bc-12) or Mshindi (bc-12), and would complement the major resistance gene bc-12. A quantitative trait loci (QTL) study done on these RILs confirmed the presence of bc-12 gene (SS715647636 QTL) on chromosome PV03 as expected. However, another QTL (SS715645701) with a smaller effect was found on Pv09. Interestingly, the positive (resistant) allele at this QTL came from RWV 3006, the susceptible parent. Results from this study showed that the combination of both QTLs increases resistance, either at the heterozygous or homozygous state. It sets the stage for further surveys of minor viral resistance genes in common-bean germplasm. To facilitate future breeding efforts, in the third chapter I attempted to obtain tightly-linked markers useful for marker-assisted selection (MAS) of the bc-12 gene in the presence of other epistatic virus resistance genes. These markers should be more broadly applicable to different genetic backgrounds, reproducible across laboratories, and able to distinguish homozygotes from heterozygotes (co-dominant). Based on a known SNP marker linked to bc-12 resistance gene, we developed a dCAPS (derived Cleaved Amplified Polymorphic Sequence) marker. However, it was not possible to clearly visualize the polymorphism generated by the designed dCAPSHindIII marker on agarose gels in all tested genotypes. This may be explained by the small size of the marker. The low resolution of the agarose gel could not detect small size differences as effectively as polyacrylamide gel electrophoresis, which can distinguish fragments with few (one to five) base pairs difference. We suggest that the marker should be tested in a larger number of different bean seed types to ascertain its usefulness in different bean classes. Furthermore, in future work with this dCAPSHindIII marker, the use of polyacrylamide gel could facilitate effective discrimination between DNA fragments from the resistant and susceptible parents.
Download or read book Identification of Necrosis inducing Strains of Bean Common Mosaic Virus and Breeding for Resistance written by Milton A. Morales Lopez and published by . This book was released on 1983 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bean Common Mosaic written by Francisco José Morales and published by CIAT. This book was released on 1989 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Natural Resistance Mechanisms of Plants to Viruses written by Gad Loebenstein and published by Springer Science & Business Media. This book was released on 2007-05-26 with total page 547 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a first attempt to link well-known plant resistance phenomena with emerging concepts in molecular biology. Resistance phenomena such as the local lesion response, induced resistance, "green islands" and resistance in various crop plants are linked with new information on gene-silencing mechanisms, gene silencing suppressors, movement proteins and plasmodesmatal gating, downstream signalling components, and more.
Download or read book Genetic Resources of Phaseolus Beans written by Paul Gepts and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: The period following the second world war has witnessed an expanding commitment to incr~ased food production in tropical countries. Public and private initiatives at the national and international levels have led to the creation of programs geared specifically towards the improvement of food crops in tropical conditions. Examples of this increased commitment are the network of international agricultural research centers and numerous bilateral aid projects. As a consequence, crop improvement has become a truly worldwide endeavor, relying on an international network of institutions and collaborators. This holds also for Phaseolus beans. Following the discovery of the Americas, Phaseolus beans became distributed on all six continents. Yet, until not so long ago, most of the research on Phaseolus improvement took place in developed countries. In recognition of the nutritional importance of Phaseolus beans in developing countries, this has changed considerably in the last years, principally perhaps through the activities of the Centro Internacional de Agricultura Tropical (CIAT) and the International Board for Plant Genetic Resources (IBPGR). Consequently, the scope of the research on Phaseolus has broadened considerably and the number of Phaseolus researchers is larger than ever before.
Download or read book CRC Handbook of Viruses Infecting Legumes written by John R. Edwardson and published by CRC Press. This book was released on 2018-01-18 with total page 860 pages. Available in PDF, EPUB and Kindle. Book excerpt: This interesting handbook discusses 145 plant viruses in 27 groups and 31 unclassified viruses in naturally infected legumes. The viruses were observed in field infections of 281 species in 64 genera of the Leguminosae. The book presents information regarding resistance sources and resistance-breeding, vectors, seed transmission, and host ranges. Measurements of virus properties are organized in tabular form for particle dimensions, serological relationships, nucleic acid percentages, sedimentation coefficients of particles and nucleic acids, molecular weights of nucleic acids and coat proteins, optical density, and buoyant density. Handbook of Viruses Infecting Legumes is unique in that it relates inclusion cytology to plant virus detection, identification, and classification. Light and electron micrographs illustrate morphology, location, and staining reactions of inclusions. Of the 27 groups that contain viruses infecting legumes in nature, inclusions are diagnostic at the group level in 15 of these groups. Plant breeders, diagnosticians, plant virologists, and students of plant virology will find this an indispensable guide to legume viruses.
Download or read book Breeding for Resistance to Bean Common Mosaic Necrosis Virus and Molecular Tagging of Bc 3 Gene in Common Bean written by Mukeshimana. Gérardine and published by . This book was released on 2003 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Breeding Reviews Volume 10 written by Jules Janick and published by John Wiley & Sons. This book was released on 2010-04-22 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: Part of a series which presents papers of topical interest relating to the breeding of plants important to agriculture and horticulture.
Download or read book Mechanisms of Resistance to Plant Diseases written by R.S. Fraser and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant resistance to pathogens is one of the most important strategies of disease control. Knowledge of resistance mechanisms, and of how to exploit them, has made a significant contribution to agricultural productivity. However, the continuous evolution of new variants of pathogen, ana additional control problems posed by new crops and agricultural methods, creates a need for a corresponding increase in our understanding of resistance and ability to utilize it. The study of resistance mechanisms also has attractions from a purely academic point of view. First there is the breadth of the problem, which can be approached at the genetical, molecular, cellular, whole plant or population lev~ls. Often there is the possibility of productive exchange of ideas between different disciplines. Then there is the fact that despite recent advances, many of the mechanisms involved have still to be fully elucidated. Finally, and compared with workers in other areas of biology, the student of resistance is twice blessed in having as his subject the interaction of two or more organisms, with the intriguing problems of recognition, specificity and co-evolution which this raises.
Download or read book Plant Resistance to Viruses written by David Evered and published by John Wiley & Sons. This book was released on 2008-04-30 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concern about the environmental consequences of the widespread use of pesticides has increased, and evidence of pesticide-resistant virus vectors have continued to emerge. This volume presents a timely survey of the mechanisms of plant resistance and examines current developments in breeding for resistance, with particular emphasis on advances in genetic engineering which allow for the incorporation of viral genetic material into plants. Discusses the mechanisms of innate resistance in strains of tobacco, tomato, and cowpea; various aspects of induced resistance, including the characterization and roles of the pathogenesis-related proteins; antiviral substances and their comparison with interferon; and cross-protection between plant virus strains. Also presents several papers which evaluate the status of genetic engineering as it relates to breeding resistant plants. Among these are discussions of the potential use of plant viruses as gene vectors, gene coding for viral coat protein, satellite RNA, and antisense RNA, and practical issues such as the durability of resistant crop plants in the field.
Download or read book Determining the Mechanism of the Recessive Resistance to Bean Common Mosaic Virus BCMV Conferred by the Bc 1 and Bc 2 Recessive Genes in Common Bean and Biological and Molecular Characterization of a New Strain of BCMV written by Gardenia Edith Orellana Arreaga and published by . This book was released on 2019 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bean common mosaic virus (BCMV) is an important pathogen affecting common bean and other legumes. BCMV is an aphid-transmitted virus, which can also be seed-transmissible in common bean with efficiencies of up to 80%, depending on virus strain and bean cultivar. The virus belongs to the genus Potyvirus (family Potyviridae), having an approximately 10-kb single-stranded, positive-sense RNA genome. BCMV resistance in common bean is governed by one dominant (I) gene and four recessive (bc-u, bc-1, bc-2, and bc-3) genes which are deployed by breeders in different combinations to protect commercial bean cultivars against the virus. The dominant I gene confers extreme resistance or immunity against all strains of BCMV when the temperature stays below 30°C, and variable types of local and systemic necrosis when temperature exceeds 30°C. The bc-3 gene was identified as an eIF4E translation initiation factor gene mediating resistance through disruption of the interaction between this protein and the VPg protein of the virus. The mode of action of bc-1 and bc-2 in expression of BCMV resistance was unknown until this work. To investigate the possible role of both bc-1 and bc-2 genes in replication, cell-to-cell, and long distance movement of BCMV in P. vulgaris, we tested virus spread of eight BCMV isolates representing pathogroups I, IV, VI, VII, and VIII, in a set of bean differentials expressing different combinations of six resistance alleles including bc-u, bc-1, bc-12, bc-2, bc-22, and bc-3. The data suggest that bc-1 and bc-2 recessive resistance genes have no effect on the replication and cell-to-cell movement of BCMV, but affect systemic spread of BCMV in common bean. The BCMV resistance conferred by bc-1 and bc-2 and affecting systemic spread was found only partially effective when these two genes were expressed singly. The efficiency of the restriction of the systemic spread of the virus was greatly enhanced when the alleles of bc-1 and bc-2 genes were combined together.Beside common bean, there are other crop legumes that are susceptible to BCMV, such as peanut, soybean, and azuki bean. In this thesis, I described a new strain of BCMV from lima bean with distinct biological and molecular characteristics found in Honolulu, HI. This new BCMV strain, BCMV-A1, was able to partially overcome resistance to the virus conferred by bc-1 and bc-2 alleles in common bean, establishing an asymptomatic systemic infection in some common bean cultivars. Later, two BCMV isolates were found in commercial common bean samples in Idaho exhibiting 99% identity to BCMV-A1 in partial genome sequence. This new BCMV strain from lima bean, BCMV-A1, may present a threat for the common bean production.
Download or read book Polyploidy and Hybridization for Crop Improvement written by Annaliese S. Mason and published by CRC Press. This book was released on 2017-11-22 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many of our current agricultural crops are natural or agricultural hybrids (between two or more species), or polyploids (containing more than one genome or set of chromosomes). These include potato, oats, cotton, oilseed rape, wheat, strawberries, kiwifruit, banana, seedless watermelon, triticale and many others. Polyploidy and hybridization can also be used for crop improvement: for example, to introgress disease resistance from wild species into crops, to produce seedless fruits for human consumption, or even to create entirely new crop types. Some crop genera have hundreds of years of interspecific hybridization and ploidy manipulation behind them, while in other genera use of these evolutionary processes for crop improvement is still at the theoretical stage. This book brings together stories and examples by expert researchers and breeders working in diverse crop genera, and details how polyploidy and hybridization processes have shaped our current crops, how these processes have been utilized for crop improvement in the past, and how polyploidy and interspecific hybridization can be used for crop improvement in the future.
Download or read book Recognition and Response in Plant Virus Interactions written by Ronald S.S. Fraser and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mechanisms of resistance to plant viruses are diverse, and probably involve different types of recognition events. Often, a cascade of changes affecting broader aspects of defence and metabolism is switched on progressively after the initial recognition event. Virulence, i.e. resistence-breaking behaviour of the virus, involves a failure or alteration of recognition or subsequent signalling. Consequences of these recognition events are the ways in which the pathogenic effects on the host are exerted: formation of visible symptoms and control of plant growth. This volume offers a comprehensive coverage of the recognition and signalling events between plants and viruses whereby the particular attraction of viruses (and viroids) is that they can now be completely defined in molecular terms: they offer excellent opportunities for studying the molecular biology of signalling, and may even provide useful guidelines on how plants and cellular pathogens interact.
