Download or read book Genetic and Functional Analysis of Conjugated Linoleic Acid Production in Bifidobacteria and Lactic Acid Bacteria written by Eva Rosberg-Cody and published by . This book was released on 2008 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: The overall aims of this thesis were to gain an increased insight into the role and implications of bacterial CLA production both in relation to host health, and also examine the physiological role of CLA production from the bacteria point of view. Initially, we screened neonatal bifidobacteria from faeces of infants with different case histories, e. g full-term/preterm, breast-/formula-fed, in order to isolate novel CLA producing strains. The most commonly isolated strain was Bifidobacterium breve, which were also the most efficient CLA producers. In a subsequent study, we localized, amplified, cloned and overexpressed the putative c9, t11 CLA isomerase from Bifidobacterium breve in both Lactococcus lactis and Corynebacterium glutamicum. The resultant recombinant strains however, showed no isomerise activity, but did convert linoleic acid to hydroxy fatty acids, believed to be intermediates in CLA production. Interestingly, structural comparisons revealed that the putative c9, t11 isomerase and the t10, c12 isomerase from Propionibacterium acnes have almost identical structures. In addition, clones expressing the putative c9, t11 CLA isomerise exhibited increased tolerance to a number of environmental stress including heat and that associated with solvent exposure. In another study, the t10, c12 CLA isomerase from Propionibacterium acnes was cloned and overexpressed in Lactococcus lactis and Escherichia coli. The resultant recombinant strains could convert the substrate linoleic acid to t10, c12 CLA. Indeed, t10, c12 CLA extracted from recombinant lactococci was shown to be strongly anti-proliferative against SW480 colon cancer cells. To investigate if the observed increased tolerance to stress by recombinant strains carrying the putative c9, t11 CLA isomerase also could be observed for clones carrying the active t10, c12 CLA isomerase, recombinant Lactococcus lactis heterologously expressing the t10, c12 CLA isomerase, along with vector controls of Lactococcus were exposed to normally lethal levels of heat, solvent and salt stresses. The results demonstrated that recombinant Lactococcus expressing the isomerase displayed a 100-fold increased survival, which was even more pronounced in the presence of substrate linoleic acid. This increased tolerance to environmental stresses of strains which express the isomerase may be due to changes in membrane fluidity as a result of incorporation of conjugated fatty acids. Finally, the active t10, c12 CLA isomerase was cloned and overexpressed in probiotic Lactobacillus paracasei NFBC 338 (Lb 338) and used in a mice feeding trial together with the substrate linoleic acid. This resultant strain produced CLA in vivo, as shown by a 4-fold increase of the conjugated isomer in adipose tissue compared with mice fed a control strain. To investigate the impact of pure t10, c12 CLA on mice fatty acid profile, this isomer was fed to mice which resulted in many changes in the fatty acid profile, most notably, a 300-700 % increase in the beneficial omega-3 fatty acids, EPA, DPA and DHA in most tissues.
Download or read book Lactic Acid Bacteria written by Marcela Albuquerque Cavalcanti de Albuquerque and published by CRC Press. This book was released on 2020-02-19 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lactic acid bacteria (LAB) are a diverse group of bacteria that comprise low GC content Gram-positive cocci or rods that produces lactic acid as the major end product of the fermentation process. Bifidobacterium genera may also be considered as a part of the LAB group for possessing some similar phenotypical characteristics despite the higher GC content. The key feature of LAB metabolism is efficient carbohydrate fermentation. This contributes to the production of several microbial metabolites that result in the improvement of flavor and texture of fermented foods, in addition to its positive impact on the human health when LAB is administered as a probiotic. The book deals with advances made in the functionalities of LAB, such as their effect on vitamin D receptor expression, impact on neurodegenerative pathologies, production of B-vitamins for food bio-enrichment, production of bacteriocins to improve gut microbiota dysbiosis, production of metabolites from polyphenols and their effects on human health, effect on reducing the immunoreaction of food allergens, as biological system using time-temperature to improve food safety, and the use of probiotics in animal feed. The book also reviews the use of LAB and probiotic technologies to develop new functional foods and functional pharmaceuticals.
Download or read book Lactic Acid Bacteria written by Gabriel Vinderola and published by CRC Press. This book was released on 2019-04-08 with total page 744 pages. Available in PDF, EPUB and Kindle. Book excerpt: Through four editions, Lactic Acid Bacteria: Microbiological and Functional Aspects, has provided readers with information on the how’s and why’s lactic acid-producing fermentation improves the storability, palatability, and nutritive value of perishable foods. Thoroughly updated and fully revised, with 12 new chapters, the Fifth Edition covers regulatory aspects globally, new findings on health effects, properties and stability of LAB as well as production of target specific LAB. The new edition also addresses the technological use of LAB in various fermentations of food, feed and beverage, and their safety considerations. It features the detailed description of the main genera of LAB as well as such novel bacteria as fructophilic LAB and novel probiotics and discusses such new targets as cognitive function, metabolic health, respiratory health and probiotics. Key Features: In 12 new chapters, findings are presented on health effects, properties and stability of LAB as well as production of target specific LAB Covers such novel bacteria as fructophilic LAB and novel probiotics Presents new discoveries related to the mechanisms of lactic acid bacterial metabolism and function Covers the benefits of LAB, both in fermentation of dairy, cereal, meat, vegetable and silage, and their health benefits on humans and animals Discusses the less-known role of LAB as food spoilers Covers the global regulatory framework related to safety and efficacy
Download or read book Lactic Acid Bacteria written by Sampo Lahtinen and published by CRC Press. This book was released on 2011-12-13 with total page 800 pages. Available in PDF, EPUB and Kindle. Book excerpt: While lactic acid-producing fermentation has long been used to improve the storability, palatability, and nutritive value of perishable foods, only recently have we begun to understand just why it works. Since the publication of the third edition of Lactic Acid Bacteria: Microbiological and Functional Aspects, substantial progress has been made in a number of areas of research. Completely updated, the Fourth Edition covers all the basic and applied aspects of lactic acid bacteria and bifidobacteria, from the gastrointestinal tract to the supermarket shelf. Topics discussed in the new edition include: Revised taxonomy due to improved insights in genetics and new molecular biological techniques New discoveries related to the mechanisms of lactic acid bacterial metabolism and function An improved mechanistic understanding of probiotic functioning Applications in food and feed preparation Health properties of lactic acid bacteria The regulatory framework related to safety and efficacy Maintaining the accessible style that made previous editions so popular, this book is ideal as an introduction to the field and as a handbook for microbiologists, food scientists, nutritionists, clinicians, and regulatory experts.
Download or read book Probiotic Dairy Products written by Adnan Y. Tamime and published by John Wiley & Sons. This book was released on 2008-04-15 with total page 235 pages. Available in PDF, EPUB and Kindle. Book excerpt: Following significant developments in recent understanding of milksystems and an explosion in interest in the use of probiotics andprebiotics as functional foods Probiotic Dairy Productslooks at advancements in the dairy industry and reviews the latestscientific developments in regard to the ‘functional’aspects of dairy and fermented milk products and their ingredients.The first title in Blackwell Publishing’s prestigiousSociety of Dairy Technology Series, this key text includescoverage of: Production systems Gut microflora Genomic characterisation of probiotic micro-organisms Physical properties associated with health claims Maintenance of the viability of probiotic products National and international statutory regulations Edited by Adnan Tamime, with contributions from internationalauthors and full of core commercially useful information for thedairy industry, this book is an essential title for dairyscientists, dairy technologists and nutritionists worldwide.
Download or read book Index Medicus written by and published by . This book was released on 2003 with total page 2084 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book Bifidobacteria written by Douwe van Sinderen and published by Humana. This book was released on 2021-03-03 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides current protocols that can be used in various experimental settings involving bifidobacteria. Chapters guide readers through experimental protocols on procedures to isolate and cultivate bifidobacteria, taxonomic identification of bifidobacterial isolates, sequencing and annotate genomes, physiologically characterize bifidobacteria, and methods on the genetic manipulation of bifidobacterial strains. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bifidobacteria: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
Download or read book New and Future Developments in Microbial Biotechnology and Bioengineering written by Alexandre Gomes Rodrigues and published by Elsevier. This book was released on 2020-06-10 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Biomolecules: Properties, Relevance and Their Translational Applications presents a concise review on microbial biotechnology, along with impacts and recent results from research centers, small companies and large enterprises. The book brings the most relevant information on how we can use resources - in this case from microorganisms - and technology to develop solutions in fields like biofuels, food, cosmetics and medicine. It covers case studies of start-ups in the field and explains how scientists have moved their ideas into profitable bio-based products that are necessary for our current living standards. In addition, the book describes strategic governmental programs designed to exploit biomass in a sustainable way, along with detailed information on research in several high-impact, worldwide laboratories. It gives concrete examples of ongoing research from molecules to methods, such as L-asparaginase, extremophiles, new diagnostics tools and the analytical methods that have raised the quality of the data obtained, thereby boosting the so-called bioeconomy. Comprises a unique source of information on the various applications of microbial biomolecules Provides resourceful material for new ideas and strong rational/application-oriented stories Discusses biotech companies in various areas (biofuel, food, medicine, etc.) who are actively using microbial biomolecules Outlines scientific discoveries and their translation into profitable products Gives an insight perspective of institutional and governmental strategic research programs aiming to preserve, explore and generate benefits from microbial biomolecules
Download or read book Lactic Acid Bacteria written by Wei Chen and published by Springer. This book was released on 2019-07-16 with total page 283 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces readers to basic studies on and applied techniques involving lactic acid bacteria, including their bioengineering and industrial applications. It summarizes recent biotechnological advances in lactic acid bacteria for food and health, and provides detailed information on the applications of these bacteria in fermented foods. Accordingly, it offers a valuable resource for researchers and graduate students in the fields of food microbiology, bioengineering, fermentation engineering, food science, nutrition and health.
Download or read book Probiotic Dairy Products written by Adnan Y. Tamime and published by John Wiley & Sons. This book was released on 2018-02-05 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Probiotic Dairy Products, 2nd Edition The updated guide to the most current research and developments in probiotic dairy products The thoroughly revised and updated second edition of Probiotic Dairy Products reviews the recent advancements in the dairy industry and includes the latest scientific developments in regard to the 'functional' aspects of dairy and fermented milk products and their ingredients. Since the publication of the first edition of this text, there have been incredible advances in the knowledge and understanding of the human microbiota, mainly due to the development and use of new molecular analysis techniques. This new edition includes information on the newest developments in the field. It offers information on the new ‘omic’ technologies that have been used to detect and analyse all the genes, proteins and metabolites of individuals’ gut microbiota. The text also includes a description of the history of probiotics and explores the origins of probiotic products and the early pioneers in this field. Other chapters in this resource provide valuable updates on genomic analysis of probiotic strains and aspects of probiotic products’ production and quality control. This important resource: Offers a completely revised and updated edition to the text that covers the topic of probiotic dairy products Contains 4 brand new chapters on the following topics: the history of probiotics, prebiotic components, probiotic research, and the production of vitamins, exopolysaccharides (EPS), and bacteriocins Features a new co-editor and a host of new contributors, that offer the latest research findings and expertise Is the latest title in Wiley's Society of Dairy Technology Technical Series Probiotic Dairy Products is an essential resource for dairy scientists, dairy technologists and nutritionists. The text includes the results of the most reliable research in field and offers informed views on the future of, and barriers to, the progress for probiotic dairy products.
Download or read book Stress Responses of Lactic Acid Bacteria written by Effie Tsakalidou and published by Springer Science & Business Media. This book was released on 2011-09-06 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: Beginning with the basics of lactic acid bacteria and stress response, then working into specific fields of research and current developments, Stress Responses of Lactic Acid Bacteria will serve as an essential guidebook to researchers in the field, industry professionals, and advanced students in the area. The exploration of stress responses in lactic acid bacteria began in the early 90s and revealed the differences that exist between LAB and the classical model microorganisms. A considerable amount of work has been performed on the main genera / species of LAB regarding the genes implicated and their actual role and regulation, and the mechanisms of stress resistance have also been elucidated. Recent genome and transcriptome analyses complement the proteome and genetic information available today and shed a new light on the perception of and the responses to stress by lactic acid bacteria.
Download or read book Food Safety and Human Health written by Ram Lakhan Singh and published by Academic Press. This book was released on 2019-07-30 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: Food Safety and Human Health provides a framework to manage food safety risks and insure safe food system. This reference takes a reader-friendly approach in presenting the entire range of toxic compounds found naturally in foods or introduced by industrial contamination or food processing methods. It provides the basic principles of food toxicology and its processing and safety for human health to help professionals and students better understand the real problems of toxic materials. This essential resource will help readers address problems regarding food contamination and safety. It will be particularly useful for graduate students, researchers and professionals in the agri-food industry. Encompasses the first pedagogic treatment of the entire range of toxic compounds found naturally in foods or introduced by industrial contamination or food processing methods Features areas of vital concern to consumers, such as the toxicological implications of food, implications of food processing and its safety to human health Focuses on the safety aspects of genetically modified foods currently available
Download or read book Lactic Acid Bacteria written by Wei Chen and published by Springer. This book was released on 2019-08-08 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the latest research and new techniques in the field of lactic acid bacteria, including comparative genomics, transcriptomics, proteomics and metabolomics. It also introduces the omics and functional evaluation in detail and shows the links between lactic acid bacteria and gut health and host immunity. Summarizing the biotechnological advances in lactic acid bacteria for food and health, it is a valuable resource for researchers and graduate students in the fields of food microbiology, bioengineering, food science, nutrition and health.
Download or read book Industrial Biotechnology written by Christoph Wittmann and published by John Wiley & Sons. This book was released on 2017-04-10 with total page 789 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest volume in the Advanced Biotechnology series provides an overview of the main production hosts and platform organisms used today as well as promising future cell factories in a two volume book. Alongside describing tools for genetic and metabolic engineering for strain improvement, the authors also impart topical information on computational tools, safety aspects and industrial-scale production. Following an introduction to general concepts, historical developments and future technologies, the text goes on to cover multi-purpose bacterial cell factories, including those organisms that exploit anaerobic biosynthetic power. Further chapters deal with microbes used for the production of high-value natural compounds and those obtained from alternative raw material sources, concluding with eukaryotic workhorses.
Download or read book Starter Cultures in Food Production written by Barbara Speranza and published by John Wiley & Sons. This book was released on 2017-02-06 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: Starter cultures have great significance in the food industry due to their vital role in the manufacture, flavour, and texture development of fermented foods. Once mainly used in the dairy industry, nowadays starter cultures are applied across a variety of food products, including meat, sourdough, vegetables, wine and fish. New data on the potential health benefits of these organisms has led to additional interest in starter bacteria. Starter Cultures in Food Production details the most recent insights into starter cultures. Opening with a brief description of the current selection protocols and industrial production of starter cultures, the book then focuses on the innovative research aspects of starter cultures in food production. Case studies for the selection of new starter cultures for different food products (sourdough and cereal based foods, table olives and vegetables, dairy and meat products, fish and wine) are presented before chapters devoted to the role of lactic acid bacteria in alkaline fermentations and ethnic fermented foods. This book will provide food producers, researchers and students with a tentative answer to the emerging issues of how to use starter cultures and how microorganisms could play a significant role in the complex process of food innovation.
Download or read book Index to Theses with Abstracts Accepted for Higher Degrees by the Universities of Great Britain and Ireland and the Council for National Academic Awards written by and published by . This book was released on 2009 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Yeast Diversity in Human Welfare written by Tulasi Satyanarayana and published by Springer. This book was released on 2017-05-13 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book brings together and updates the latest information on the diversity of yeasts, their molecular features and their applications in the welfare of mankind. Yeasts are eukaryotic microfungi widely found in natural environments, including those with extreme conditions such as low temperatures, low oxygen levels and low water availability. To date, approximately 2,000 of the estimated 30,000 to 45,000 species of yeast on Earth, belonging to around 200 genera have been described. Although there are a few that are opportunistic human and animal pathogens, the vast majority of yeasts are beneficial, playing an important role in the food chain and in the carbon, nitrogen and sulphur cycles. In addition, yeasts such as Saccharomyces cerevisiae, Hansenula polymorpha and Pichia pastoris are used in expressing foreign genes to produce proteins of pharmaceutical interest. A landmark in biotechnology was reached in 1996 with the completion of sequencing of the entire S. cerevisiae genome, and it has now become a central player in the development of an entirely new approach to biological research and synthetic biology. The sequencing of genomes of several yeasts including Schizosaccharomyces pombe, Candida albicans and Cryptococcus neofromans has also recently been completed. candida albicans="" and="" p/pp
