Download or read book Functional Characterization of JAG1 in Prostate Cancer written by and published by . This book was released on 2016 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Functional Characterization of Two Novel Human Prostate Cancer Metastasis Related Genes written by and published by . This book was released on 2005 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt: We propose to identify the functional characterization of two novel cancer-specific, metastasis-related genes whose constitutive expression may be pivotal for prostate cancer progression. Work accomplished was performed based on the proposed statement of work. We have characterized the full-length cDNAs of the Seq1 and Seq2 genes using at least three 5' and '3 rapid amplifications of cDNA ends (RACE) commercial kits (Invitrogen Carlsbad, CA, BD Bioscience (Clontech Inc), and Seegene, Rockville, MD). To optimize the PCR conditions for each kit, we had designed several sets of gene-specific primers (GSP;23-28 nt long) with 50-70% GC and Tm of 55 to 75 degrees C for each gene. We have also designed several sets of nested GSPs to verify our cloned genes. Because of unique secondary structures, high GC content, short SSH sequences, and low levels of expression of these genes in prostate cancer cell lines, we had great deal of difficulty in accomplishing this task in a timely fashion. As such, we devised different strategies for the first-strand synthesis using a modified oligo(dT) primers (5'-CDS primer or 3'-CDS primer), and Smart oligo II primer under various conditions. The full-length cDNA sequences were subcloned into mammalian expression vectors (Invitrogen) and ready to be used for generation of recombinant proteins and antibody production.
Download or read book Differentially Expressed Proteins in Prostate Cancer and Functional Characterization of Proteins with Altered Expression written by Ramesh Ummanni and published by . This book was released on 2008 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Clinic and Functional Analysis of P73R1 Mutations in Prostate Cancer written by and published by . This book was released on 2006 with total page 40 pages. Available in PDF, EPUB and Kindle. Book excerpt: The DNA damage-signaling pathway has been implicated in the development of prostate cancer since germline mutations in several genes (BRCA1, BRCA2, and CHEK2) whose products are involved in this pathway have been associated with increased risk for this cancer. We previously isolated a novel p73 up-regulated gene (p73R1) and identified p73R1 mutations in prostate cancer. In this report, we screened 856 unselected prostate cancer specimens and detected a frequency of 2.6% (221856) truncation mutations in prostate cancers in contrast to 0.6% (21327) in 327 population-based controls (Fishers exact test, P = 0.036), with an odds ratio of 4.3 (95% confidence interval 1.2 - 21.2). In addition, we also demonstrated that mutant p73R1 was unable to induce apoptosis and suppress cell growth in HeLa and Cos7 cells. The loss of function mutation in p73R1 is due to the inability of the mutant to induce cytochrome c release from mitochondria. These results suggest that loss of function mutations in p73R1 predispose men to prostate cancer and further support the concept that the genetic defects in the DNA damage-response genes play an important role in the development of prostate cancer.
Download or read book Functional Characterization of Common Prostate Cancer Risk Variants written by Xing Xu and published by . This book was released on 2014 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Functional Characterization of a Long Non coding RNA Associated with Prostate Cancer written by Md Faqrul Hasan and published by . This book was released on 2019 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prostate cancer is the most common cancer in men in the western world. Although early stage prostate cancer is treatable late stage, more specifically, metastatic and drug resistant prostate cancers are mostly incurable. The failure of current treatments obligates the research community to explore novel areas in prostate cancer biology and find better therapeutic targets. Emerging evidences show that non-coding RNAs specifically long non-coding RNAs (lncRNAs) play regulatory roles in various cellular processes and are frequently dysregulated in cancer including prostate cancer. These aberrantly expressed lncRNAs mostly with unexplored genetic information may drive cancer progression. Previous studies done in our laboratory showed a tumor suppressor role of a cluster of small non-coding RNAs or microRNA (miRNA) miR-17-92a in PC-3 prostate cancer cells. To learn the underlying mechanism, transcriptome analysis with or without expression of miR-17-92a was conducted in our laboratory. RNA-sequencing data analysis identified reduced expression of a set of lncRNAs and oncogenes, and up regulation of several tumor suppressor genes upon expression of miR-17-92a cluster miRNAs. One of the down regulated intergenic lncRNAs, PAINT (Prostate Cancer Associated Intergenic Non-coding Transcript) (LINC00888), was selected for determining its functional role in prostate cancer. TCGA and GEO profiles analyses revealed up regulation of PAINT in prostate tumors with higher Gleason Scores, in highly aggressive metastatic prostate cancer cell lines, and upon androgen deprivation therapy of prostate cancer cells.
Download or read book Identification and Functional Characterization of Novel Markers for Prostate Cancer written by Alexander Hermani and published by . This book was released on 2005 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Functional Characterization of Prostate Cancer Biomarkers written by Josefine Gerhardt and published by . This book was released on 2011 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterizing the Multi faceted Dynamics of Tumor Cell Plasticity written by Satyendra Chandra Tripathi and published by Frontiers Media SA. This book was released on 2021-03-01 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Signal Transduction in Cancer Metastasis written by Wen-Sheng Wu and published by Springer Science & Business Media. This book was released on 2010-10-04 with total page 293 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines the signal mechanisms responsible for triggering a series of phenotypical changes of primary tumor which may lead to final colonization of the tumor in a second home. It highlights the initial stage of tumor metastasis.
Download or read book Prostate Cancer written by Leland W. K. Chung and published by Springer Science & Business Media. This book was released on 2007-11-10 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prostate Cancer: Biology, Genetics, and the New Therapeutics, Second Edition, reviews new, valuable approaches to the treatment of prostate cancer in men. The latest edition contains new material on molecular imaging, new treatments for prostate cancer, molecular targets, cell signaling pathways, bioinformatics, and pathogenomics. The book details the latest innovations and advances in prostate cancer and may be used as a rapid reference text for readers. The volume profiles the latest advances in cancer research and treatment and includes profound studies in prostate stem cells, cancer-host interactions, hedgehog signaling in development and cancer, cholesterol and cell signaling, gene therapy for advanced prostate cancer, and noninvasive strategies such as molecular imaging to visualize gene expression. This new edition also investigates expression profiling and somatic alterations in prostate cancer progression and linkage studies of prostate cancer families to identify susceptibility genes. The issues of racial differences in prostate cancer mortality, radiotherapy for the treatment of locally advanced prostate cancer, recombinant antibody candidates for treatment, taxane-based chemotherapy, lethal phenotypes, and novel and efficient translation clinical trials are also presented in great depth. Prostate Cancer: Biology, Genetics, and the New Therapeutics, Second Edition, provides readers with a general reference for prostate cancer from prevention to therapy and will be of value to clinicians, scientists, and administrators who strive to solve the cancer problem.
Download or read book Genetics of Bone Biology and Skeletal Disease written by Rajesh V. Thakker and published by Academic Press. This book was released on 2017-10-31 with total page 880 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetics of Bone Biology and Skeletal Disease, Second Edition, is aimed at students of bone biology and genetics and includes general introductory chapters on bone biology and genetics. More specific disease orientated chapters comprehensively summarize the clinical, genetic, molecular, animal model, molecular pathology, diagnostic, counseling, and treatment aspects of each disorder. The book is organized into five sections that each emphasize a particular theme, general background to bone biology, general background to genetics and epigenetics, disorders of bone and joint, parathyroid and related disorders, and vitamin D and renal disorders. The first section is specifically devoted to providing an overview of bone biology and structure, joint and cartilage biology, principles of endocrine regulation of bone, and the role of neuronal regulation and energy homeostasis. The second section reviews the principles and progress of medical genetics and epigenetics related to bone disease, including genome-wide association studies (GWAS), genomic profiling, copy number variation, prospects of gene therapy, pharmacogenomics, genetic testing and counseling, as well as the generation and utilizing of mouse models. The third section details advances in the genetics and molecular biology of bone and joint diseases, both monogenic and polygenic, as well as skeletal dysplasias, and rarer bone disorders. The fourth section highlights the central role of the parathyroids in calcium and skeletal homeostasis by reviewing the molecular genetics of: hyperparathyroidism, hypoparathyrodism, endocrine neoplasias, and disorders of the PTH and calcium-sensing receptors. The fifth section details molecular and cellular advances across associated renal disorders such as vitamin D and rickets. Identifies and analyzes the genetic basis of bone disorders in humans and demonstrates the utility of mouse models in furthering the knowledge of mechanisms and evaluation of treatments Demonstrates how the interactions between bone and joint biology, physiology, and genetics have greatly enhanced the understanding of normal bone function as well as the molecular pathogenesis of metabolic bone disorders Summarizes the clinical, genetic, molecular, animal model, molecular pathology, diagnostic, counseling, and treatment aspects of each disorder
Download or read book Advances in Molecular and Pharmacological Mechanisms of Novel Targeted Therapies for Melanoma written by Luana Mota Ferreira and published by Frontiers Media SA. This book was released on 2024-04-15 with total page 129 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malignant melanoma is the most aggressive type of skin cancer with invasive growth patterns. Recent advances in melanoma management have focused on cell growth, proliferation, migration, and survival biomarkers. Numerous FDA-approved molecular-targeted therapies, such as tyrosine kinase inhibitors (TKIs), have been developed targeting genetic biomarkers that play a critical role in tumorigenesis when overexpressed. The use of targeted therapies as an alternative or adjuvant to immunotherapy has revolutionized the management of metastatic melanoma. Although this treatment strategy is more efficacious and less toxic than traditional therapies, targeted therapies are less effective after prolonged treatment due to acquired resistance given the mutations and activation of resistant mechanisms in melanoma tumors. In this sense, identifying novel target therapies and repositioned drugs that could modulate the disease progression, the potential molecular and pharmacological mechanisms of therapies are of utmost interest.
Download or read book Altered Expression of Proteins in Cancer Function and Potential Therapeutic Targets Volume II written by João Pessoa and published by Frontiers Media SA. This book was released on 2023-08-02 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Principles of Bone Biology written by John P. Bilezikian and published by Academic Press. This book was released on 2019-08-22 with total page 2024 pages. Available in PDF, EPUB and Kindle. Book excerpt: Preface from the first edition (1996): "The world of modern science is undergoing a number of spectacular events that are redefining our understanding of ourselves. As with any revolution, we should take stock of where we have been, where we are, and where we are going. Our special world of bone biology is participating in and taking advantage of the larger global revolution in modern science... we assembled experts from all over the world and asked them to focus on the current state of knowledge and the prospects for new knowledge in their area of expertise. To this end, Principles of Bone Biology was conceived." - John P. Bilezikian, Lawrence G. Raisz, Gideon A. Rodan Praise for the previous edition: "Students, teachers, and practitioners will benefit from reading it, and investigators will use it as a reference work; it will certainly be consulted frequently." --The New England Journal of Medicine For over two decades, "Big Gray" has been the go-to repository of knowledge in the disciplines related to bone and mineral metabolism. The fourth edition is a must-have for students new to the field; young investigators at the graduate or postgraduate level beginning their research careers; established scientists who need to keep up with the changing nature of the field, looking to enrich their own research programs, or who are changing their career direction; clinicians who want ready access to up-to-date relevant basic science. This new edition builds on the successful formula from previous editions, taking the reader from the basic elements of fundamental research to the most sophisticated concepts in therapeutics. Principles of Bone Biology, Fourth Edition provides the most comprehensive, authoritative reference on the study of bone biology and related diseases. Bone research continues to generate enormous attention, due to the broad public health implications of osteoporosis and related bone disorders. This classic, fully updated, two volume reference is designed for anyone involved in the study of bone biology. Provides a "one-stop" shopping paradise. Anything you want to find about bone biology is here and written by the world’s experts THE essential resource for anyone involved in the study of the skeleton and metabolic bone diseases Covers everything from the basic scientific concepts to the underlying principles of therapeutics and management Allows readers to easily search and locate information quickly in the online format Volumes include: Basic Principles; Molecular Mechanisms of Metabolic Bone Disease; Pharmacological Mechanisms of Therapeutics; Methods in Bone Research
Download or read book Roles of Non coding RNAs in Infectious Diseases written by Guofeng Cheng and published by Frontiers Media SA. This book was released on 2022-11-29 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Diseases of the Kidney written by Richard P. Lifton and published by Academic Press. This book was released on 2009-02-25 with total page 895 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic approaches have revolutionized our understanding of the fundamental causes of human disease by permitting the identification of specific genes in which variation causes or contributes to susceptibility to, or protection from, disease. More than 2,000 disease genes have been identified in the last 20 years, providing important new insight into the pathophysiology of diseases in every field of medicine. Genetic Diseases of the Kidney offers expert insight into the role of genetic abnormalities in the pathogenesis of abnormal kidney function and kidney disease. Genetic abnormalities are carefully presented within the appropriate physiologic context so that readers will understand not only which genes are linked to which diseases but also which pathways lead from a genetic “disturbance to the systemic appearance of disease. Lays the essential foundation of mammalian genetics principles for medical professionals with little or no background in genetics Analyzes specific renal diseases – both monogenic disorders confined to the kidney and systemic diseases with renal involvement – and explains their genetic causes World-renowned editors and authors offer expert frameworks for understanding the links between genes and complex clinical disorders (i.e., lupus, diabetes, HIV, and hypertension)
