Download or read book Fourier Ptychographic Microscopy for High resolution Large Field of View Imaging written by Eugene Egbert Fouche and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Fourier ptychographic microscopy (FPM) is an imaging technique which overcomes the limitationsof conventional microscopy to construct high-resolution, large field of view (FOV) images of a sample. Usually, there is a trade-off between resolution and field of view, but FPM allows samples to be viewed at a high resolution, while maintaining a large FOV. FPM is a computational imaging technique, where multiple low-resolution images of a sample are used to reconstruct the sample at a much higher resolution. The sample is illuminated from various angles, and a low-resolution image is captured for each illumination angle using a lens with a low numerical aperture (NA). The low NA lens has a large FOV, and the various illumination angles allows one to obtain information about the smaller sample features. This allows one to reconstruct a high-resolution, large FOV image of the sample using an iterative reconstruction algorithm. An LED array is typically used to provide the angularly varying illumination. Many real-world samples alter both the amplitude and the phase of the light that is transmitted through them. However, only the intensity can be measured on a camera, and the phase information is lost. In FPM, the various sample images allows one to recover the phase of the sample, as well as the amplitude. This can be used to correct for errors in the imaging setup, and also enhances the contrast when viewing biological samples.In this thesis, the theoretical framework behind FPM is explained, and simulations are performed to investigate the effect of the LED array size and the number of iterations of the reconstruction algorithm on the quality of the reconstructed sample. The error correction (defocus aberration) is also investigated. Two setups are constructed to investigate FPM experimentally. The first setup uses an LED array, and is used to image known calibration targets and real-world biological samples. This setup is also adapted to perform polarization-sensitive FPM (pFPM) on birefringent mineral samples to image the different crystal domains in the samples. The second setup uses a continuous wave laser as the light source and a 2-dimensional spatial light modulator (2D-SLM) to provide the angularly varying illumination. This setup is used to image a known calibration target. Both setups are characterised, and their performance is compared to illustrate their suitability for different imaging scenarios."--ENGLISH ABSTRACT.
Download or read book Fourier Ptychographic Imaging written by Guoan Zheng and published by Morgan & Claypool Publishers. This book was released on 2016-06-30 with total page 113 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book demonstrates the concept of Fourier ptychography, a new imaging technique that bypasses the resolution limit of the employed optics. In particular, it transforms the general challenge of high-throughput, high-resolution imaging from one that is coupled to the physical limitations of the optics to one that is solvable through computation. Demonstrated in a tutorial form and providing many MATLAB® simulation examples for the reader, it also discusses the experimental implementation and recent developments of Fourier ptychography. This book will be of interest to researchers and engineers learning simulation techniques for Fourier optics and the Fourier ptychography concept.
Download or read book Springer Handbook of Microscopy written by Peter W. Hawkes and published by Springer Nature. This book was released on 2019-11-02 with total page 1561 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book features reviews by leading experts on the methods and applications of modern forms of microscopy. The recent awards of Nobel Prizes awarded for super-resolution optical microscopy and cryo-electron microscopy have demonstrated the rich scientific opportunities for research in novel microscopies. Earlier Nobel Prizes for electron microscopy (the instrument itself and applications to biology), scanning probe microscopy and holography are a reminder of the central role of microscopy in modern science, from the study of nanostructures in materials science, physics and chemistry to structural biology. Separate chapters are devoted to confocal, fluorescent and related novel optical microscopies, coherent diffractive imaging, scanning probe microscopy, transmission electron microscopy in all its modes from aberration corrected and analytical to in-situ and time-resolved, low energy electron microscopy, photoelectron microscopy, cryo-electron microscopy in biology, and also ion microscopy. In addition to serving as an essential reference for researchers and teachers in the fields such as materials science, condensed matter physics, solid-state chemistry, structural biology and the molecular sciences generally, the Springer Handbook of Microscopy is a unified, coherent and pedagogically attractive text for advanced students who need an authoritative yet accessible guide to the science and practice of microscopy.
Download or read book Confocal and Two Photon Microscopy written by Alberto Diaspro and published by John Wiley & Sons. This book was released on 2001-11-22 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Confocal and Two-Photon Microscopy Foundations, Applications, and Advances Edited by Alberto Diaspro Confocal and two-photon fluorescence microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues and of other structures such as semiconductor integrated circuits. Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances provides clear, comprehensive coverage of basic foundations, modern applications, and groundbreaking new research developments made in this important area of microscopy. Opening with a foreword by G. J. Brakenhoff, this reference gathers the work of an international group of renowned experts in chapters that are logically divided into balanced sections covering theory, techniques, applications, and advances, featuring: * In-depth discussion of applications for biology, medicine, physics, engineering, and chemistry, including industrial applications * Guidance on new and emerging imaging technology, developmental trends, and fluorescent molecules * Uniform organization and review-style presentation of chapters, with an introduction, historical overview, methodology, practical tips, applications, future directions, chapter summary, and bibliographical references * Companion FTP site with full-color photographs * The significant experience of pioneers, leaders, and emerging scientists in the field of confocal and two-photon excitation microscopy Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances is invaluable to researchers in the biological sciences, tissue and cellular engineering, biophysics, bioengineering, physics of matter, and medicine, who use these techniques or are involved in developing new commercial instruments.
Download or read book Nanoscale Photonic Imaging written by Tim Salditt and published by Springer Nature. This book was released on 2020-06-09 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualization, as well as describing a range of fascinating in-depth studies. Introductory chapters cover the most relevant physics and basic methods that young researchers need to master in order to work effectively in the field of nanoscale photonic imaging, from physical first principles, to instrumentation, to mathematical foundations of imaging and data analysis. Subsequent chapters demonstrate how these cutting edge methods are applied to a variety of systems, including complex fluids and biomolecular systems, for visualizing their structure and dynamics, in space and on timescales extending over many orders of magnitude down to the femtosecond range. Progress in nanoscale photonic imaging in Göttingen has been the sum total of more than a decade of work by a wide range of scientists and mathematicians across disciplines, working together in a vibrant collaboration of a kind rarely matched. This volume presents the highlights of their research achievements and serves as a record of the unique and remarkable constellation of contributors, as well as looking ahead at the future prospects in this field. It will serve not only as a useful reference for experienced researchers but also as a valuable point of entry for newcomers.
Download or read book Chemical Imaging Analysis written by Freddy Adams and published by Elsevier. This book was released on 2015-06-06 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical Imaging Analysis covers the advancements made over the last 50 years in chemical imaging analysis, including different analytical techniques and the ways they were developed and refined to link the composition and structure of manmade and natural materials at the nano/micro scale to the functional behavior at the macroscopic scale. In a development process that started in the early 1960s, a variety of specialized analytical techniques was developed – or adapted from existing techniques – and these techniques have matured into versatile and powerful tools for visualizing structural and compositional heterogeneity. This text explores that journey, providing a general overview of imaging techniques in diverse fields, including mass spectrometry, optical spectrometry including X-rays, electron microscopy, and beam techniques. Provides comprehensive coverage of analytical techniques used in chemical imaging analysis Explores a variety of specialized techniques Provides a general overview of imaging techniques in diverse fields
Download or read book Computational Optical Phase Imaging written by Cheng Liu and published by Springer Nature. This book was released on 2022-04-11 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, computational optical phase imaging techniques are presented along with Matlab codes that allow the reader to run their own simulations and gain a thorough understanding of the current state-of-the-art. The book focuses on modern applications of computational optical phase imaging in engineering measurements and biomedical imaging. Additionally, it discusses the future of computational optical phase imaging, especially in terms of system miniaturization and deep learning-based phase retrieval.
Download or read book Coded Optical Imaging written by Jinyang Liang and published by Springer Nature. This book was released on with total page 697 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fourier Ptychographic Imaging written by Guoan Zheng and published by Morgan & Claypool Publishers. This book was released on 2016-06-30 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book demonstrates the concept of Fourier ptychography, a new imaging technique that bypasses the resolution limit of the employed optics. In particular, it transforms the general challenge of high-throughput, high-resolution imaging from one that is coupled to the physical limitations of the optics to one that is solvable through computation. Demonstrated in a tutorial form and providing many MATLAB® simulation examples for the reader, it also discusses the experimental implementation and recent developments of Fourier ptychography. This book will be of interest to researchers and engineers learning simulation techniques for Fourier optics and the Fourier ptychography concept.
Download or read book State of the Art Laser Spectroscopy and its Applications Volume II written by Yufei Ma and published by Frontiers Media SA. This book was released on 2023-02-09 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Computational Optical Imaging written by Zhengjun Liu and published by Springer Nature. This book was released on with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Communications Signal Processing and Systems written by Qilian Liang and published by Springer Nature. This book was released on 2021-06-07 with total page 2070 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book brings together papers presented at the 2020 International Conference on Communications, Signal Processing, and Systems, which provides a venue to disseminate the latest developments and to discuss the interactions and links between these multidisciplinary fields. Spanning topics ranging from communications, signal processing and systems, this book is aimed at undergraduate and graduate students in Electrical Engineering, Computer Science and Mathematics, researchers and engineers from academia and industry as well as government employees (such as NSF, DOD and DOE).
Download or read book Transmission Electron Microscopy written by C. Barry Carter and published by Springer. This book was released on 2016-08-24 with total page 518 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text is a companion volume to Transmission Electron Microscopy: A Textbook for Materials Science by Williams and Carter. The aim is to extend the discussion of certain topics that are either rapidly changing at this time or that would benefit from more detailed discussion than space allowed in the primary text. World-renowned researchers have contributed chapters in their area of expertise, and the editors have carefully prepared these chapters to provide a uniform tone and treatment for this exciting material. The book features an unparalleled collection of color figures showcasing the quality and variety of chemical data that can be obtained from today’s instruments, as well as key pitfalls to avoid. As with the previous TEM text, each chapter contains two sets of questions, one for self assessment and a second more suitable for homework assignments. Throughout the book, the style follows that of Williams & Carter even when the subject matter becomes challenging—the aim is always to make the topic understandable by first-year graduate students and others who are working in the field of Materials Science Topics covered include sources, in-situ experiments, electron diffraction, Digital Micrograph, waves and holography, focal-series reconstruction and direct methods, STEM and tomography, energy-filtered TEM (EFTEM) imaging, and spectrum imaging. The range and depth of material makes this companion volume essential reading for the budding microscopist and a key reference for practicing researchers using these and related techniques.
Download or read book Circulating Tumor Cells written by Mark Jesus M. Magbanua and published by Humana Press. This book was released on 2017-09-15 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Circula ting Tumor Cells: Methods and Protocols is a valuable resource for laboratory researchers and clinicians who are interested in furthering their studies on CTCs.
Download or read book Coherent Domain Optical Methods written by Valery V. Tuchin and published by Springer Science & Business Media. This book was released on 2004-10-15 with total page 963 pages. Available in PDF, EPUB and Kindle. Book excerpt: For the first time in one set of books, coherent-domain optical methods are discussed in the framework of various applications, which are characterized by a strong light scattering. A few chapters describe basic research containing the updated results on coherent and polarized light non-destructive interactions with a scattering medium, in particular, diffraction, interference, and speckle formation at multiple scattering. These chapters allow for understanding coherent-domain diagnostic techniques presented in later chapters. A large portion of Volume I is dedicated to analysis of various aspects of optical coherence tomography (OCT) - a very new and growing field of coherent optics. Two chapters on laser scanning confocal microscopy give insight to recent extraordinary results on in vivo imaging and compare the possibilities and achievements of confocol, excitation multiphoton, and OCT microscopy. This two volume reference contains descriptions of holography, interferometry and optical heterodyning techniques in their application for diagnostics of turbid materials. The most prospective methods of coherent and polarization optical imaging and spectroscopy, including polarization-sensitive optical coherent tomography, polarization diffusion wave spectroscopy, and elastic and quasi-elastic light scattering spectroscopies and image techniques, are presented.
Download or read book Optics Experiments and Demonstrations for Student Laboratories written by Stephen G. Lipson and published by . This book was released on 2020-08-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book on the laboratory teaching of optics is based on the author's experience during many years in several universities and colleges. It describes basic experiments in optics that are suitable for student laboratories at undergraduate and graduate levels and do not require specialized equipment or measurement techniques.
Download or read book Statistical Optics written by Joseph W. Goodman and published by John Wiley & Sons. This book was released on 2015-04-20 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses statistical methods that are useful for treating problems in modern optics, and the application of these methods to solving a variety of such problems This book covers a variety of statistical problems in optics, including both theory and applications. The text covers the necessary background in statistics, statistical properties of light waves of various types, the theory of partial coherence and its applications, imaging with partially coherent light, atmospheric degradations of images, and noise limitations in the detection of light. New topics have been introduced in the second edition, including: Analysis of the Vander Pol oscillator model of laser light Coverage on coherence tomography and coherence multiplexing of fiber sensors An expansion of the chapter on imaging with partially coherent light, including several new examples An expanded section on speckle and its properties New sections on the cross-spectrum and bispectrum techniques for obtaining images free from atmospheric distortions A new section on imaging through atmospheric turbulence using coherent light The addition of the effects of “read noise” to the discussions of limitations encountered in detecting very weak optical signals A number of new problems and many new references have been added Statistical Optics, Second Edition is written for researchers and engineering students interested in optics, physicists and chemists, as well as graduate level courses in a University Engineering or Physics Department.
