Download or read book Foundations of Nonlinear Optical Microscopy written by Eric Olaf Potma and published by John Wiley & Sons. This book was released on 2024-03-13 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: Foundations of Nonlinear Optical Microscopy Concise yet comprehensive resource presenting the foundations of nonlinear optical microscopy Foundations of Nonlinear Optical Microscopy brings together all relevant principles of nonlinear optical (NLO) microscopy, presenting NLO microscopy within a consistent framework to allow for the origin of the signals and the interrelation between different NLO techniques to be understood. The text provides rigorous yet practical derivations, which amount to expressions that can be directly related to measured values of resolution, sensitivity, and imaging contrast. The book also addresses typical questions students ask, and answers them with clear explanations and examples. Readers of this book will develop a solid physical understanding of NLO microscopy, appreciate the advantages and limitations of each technique, and recognize the exciting possibilities that lie ahead. Foundations of Nonlinear Optical Microscopy covers sample topics such as: Light propagation, focusing of light, pulses of light, classical description of light-matter interactions, and quantum mechanical description of light-matter interactions Molecular transitions, selection rules, signal radiation, and detection of light Multi-photon fluorescence and pump-probe microscopy Harmonic generation, sum-frequency generation, and coherent Raman scattering Senior undergraduate and graduate students in chemistry, physics, and biomedical engineering, along with students of electrical engineering and instructors in both of these fields, can use the information within Foundations of Nonlinear Optical Microscopy and the included learning resources to gain a concise yet comprehensive overview of the subject.

Download or read book Handbook of Biomedical Nonlinear Optical Microscopy written by Barry R. Masters and published by Oxford University Press. This book was released on 2008-05-19 with total page 895 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ideal for cell biologists, life scientists, biomedical engineers, and clinicians, this handbook provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy.

Download or read book Nonlinear Optical Microscopy written by Stefan W. Hell and published by . This book was released on 1996 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Nonlinear Optical Microscopy written by Romedi Selm and published by . This book was released on 2012 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Optical Fluorescence Microscopy written by Alberto Diaspro and published by Springer Science & Business Media. This book was released on 2010-11-25 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Download or read book Nonlinear Optical Microscopy of Highly Pigmented Tissues written by Ilyas Saytashev and published by . This book was released on 2016 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Fundamentals of Light Microscopy and Electronic Imaging written by Douglas B. Murphy and published by John Wiley & Sons. This book was released on 2012-08-22 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy

Download or read book Fundamentals of Light Microscopy written by Michael Spencer and published by CUP Archive. This book was released on 1982-07 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dr Spencer's account gives a concise but rigorous explanation of the principles underlying the various forms of light microscopy.

Download or read book A Practical Guide to Optical Microscopy written by John Girkin and published by CRC Press. This book was released on 2019-06-14 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.

Download or read book Nano optics and Near field Optical Microscopy written by Anatoly V. Zayats and published by Artech House. This book was released on 2009 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This groundbreaking book focuses on near-field microscopy which has opened up optical processes at the nanoscale for direct inspection. Further, it explores the emerging area of nano-optics which promises to make possible optical microscopy with true nanometer resolution. This frontline resource helps you achieve high resolution optical imaging of biological species and functional materials. You also find guidance in the imaging of optical device operation and new nanophotonics functionalities"--EBL.

Download or read book Exploring the Potential of Nonlinear Optical Microscopy with Tailored Femtosecond Pulses written by Lukas Brückner and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Functional Imaging by Controlled Nonlinear Optical Phenomena written by Kazuyoshi Itoh and published by John Wiley & Sons. This book was released on 2013-09-03 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ultrafast lasers allow high-precision imaging and manipulation for biological and medical applications. Nonlinear optical microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues. Nonlinear optical imaging technique is a rapidly emerging research area with widespread fundamental research and clinical applications. Nonlinear optical imaging allows both structural and functional imaging with cellular level resolution imaging in biological systems. The introduction of endogenous or exogenous probes can selectively enhance contrast for molecular targets in a living cell as well as supply functional information on processes. With the aim to control nonlinear optical processes and to obtain functional images, nonlinear optical processes can be controlled by photo-controlled probes and/or parameters of ultrafast laser pulses, such as time, space, polarization, and phase. This book gives an overview of the nonlinear optical process by ultrafast laser pulses and explains how the basics of nonlinear optical microscopy led to the most advanced techniques of photo-controlled nonlinear optical microscopy.

Download or read book Optical Imaging and Microscopy written by Peter Török and published by Springer. This book was released on 2007-08-17 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text on contemporary optical systems is intended for optical researchers and engineers, graduate students and optical microscopists in the biological and biomedical sciences. In three sections, the book discusses high-aperture optical systems, nonlinear optical techniques, and various techniques that are finding new applications. The new second edition has been thoroughly revised and expanded to account for new advances in fluorescence imaging and diffractive optical lenses.

Download or read book Non Linear Optical Materials written by R. Saravanan and published by Materials Research Forum LLC. This book was released on 2018-04-01 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chapter I provides an introduction to linear optics and the physical origin of non-linear optical phenomena. The principle characterization techniques for analyzing the microstructural, optical and morphological properties of non-linear optical materials are discussed: Powder X-ray diffraction (PXRD), UV-Visible spectroscopy, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS). Also presented are methods for the structural refinement of these materials, as well as the analysis of electron density distribution by means of novel techniques and the corresponding computational procedures. Chapter II describes sample preparation and PXRD analysis of a number of non-linear optical materials, such as PbMoO4, LiNbO3, Ce:Gd3Ga5O12, CaCO3, Yb:CaF2, and Al2O3, Cr:Al2O3,V:Al2O3. Chapter III deals with the optical properties and micro-structural characterization of non-linear optical materials, such as PbMoO4, LiNbO3, Ce:Gd3Ga5O12, CaCO3, Yb:CaF2, and Al2O3, Cr:Al2O3,V:Al2O3. The band gap, crystallite size and particle size of these materials are determined by means of UV-visible spectroscopy, powder X-ray profile analysis and scanning electron microscopy. Also discussed is the elemental compositional analysis for PbMoO4, LiNbO3, Ce:Gd3Ga5O12, CaCO3, Yb:CaF2, and Al2O3, Cr:Al2O3,V:Al2O3. Chapter IV focusses on the electron density distribution analysis of non-linear optical materials, such as PbMoO4, LiNbO3, Ce:Gd3Ga5O12, CaCO3, Yb:CaF2, and Al2O3, Cr:Al2O3,V:Al2O3. The results are presented in the form of electron density maps and profiles. The bonding behavior of these materials is studied using both quantitative and qualitative analysis. Chapter V centers on the inter-atomic ordering in non-linear optical materials, and presents computations of the pair distribution function (atomic correlation function) for selected materials.

Download or read book An Introduction to Microscopy written by Suzanne Bell and published by CRC Press. This book was released on 2009-10-21 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microscopy, which has served as a fundamental scientific technique for centuries, remains an invaluable tool in chemistry, biology, healthcare, and forensics. Increasingly, it is being integrated into modern chemical instrumentation and is of value as a powerful analytical tool across many scientific disciplines. Designed to serve as a primary reso

Download or read book Superresolution Optical Microscopy written by Barry R. Masters and published by Springer Nature. This book was released on 2020-03-21 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.

Download or read book Nonlinear Optical Microscopy for Particle Tracking and Materials Characterization written by Angela Christina Aguilar and published by . This book was released on 2019 with total page 44 pages. Available in PDF, EPUB and Kindle. Book excerpt: