Download or read book Fluorescence Fluctuation Spectroscopy FFS Part A written by and published by Academic Press. This book was released on 2012-12-28 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation SpectroscopyContains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers fluorescence fluctuation spectroscopy - Contains chapters on such topics as time-integrated fluorescence cumulant analysis, pulsed interleaved excitation, and raster image correlation spectroscopy and number and brightness analysis.
Download or read book Fluorescence Fluctuation Spectroscopy and Microscopy written by Syuan-Ming Guo and published by . This book was released on 2016 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence fluctuation spectroscopy and microscopy have been powerful tools for studying molecular organization and dynamics in cells. Fluorescence Correlation Spectroscopy (FCS) has been widely applied to probing molecular dynamics in live cells with single molecule sensitivity and the ability to resolve local molecular concentrations, aggregation states, and transport mechanisms. Despite the broad utility of FCS, interpretation of cellular FCS data is often confounded by the heterogeneity in the underlying biological process and the low signal-to-noise ratio. Systematic data evaluation and interpretation become even more challenging for imaging FCS, where hundreds to thousands of FCS curves are generated in a single measurement. This thesis presents an objective Bayesian inference procedure for testing multiple competing FCS models. Bayesian inference determines model probabilities by considering the probability distributions over the full range of parameter values, thereby naturally penalizes model complexity and prevents over-fitting. We applied this procedure to imaging FCS data in order to resolve hIAPP-induced microdomain spatial organization and temporal dynamics in the cell membrane. Our analysis resolved the temporal evolution of multiple diffusing species in the spatially heterogeneous cell membrane, lending support to the "carpet model" for the association mode of hIAPP aggregates with the plasma membrane. Finally, we presented a fluctuation-based microscopy approach, Points Accumulation for Imaging in Nanoscale Topography (PAINT), that enables highly multiplexed super-resolution imaging of synaptic proteins. We employed DNA-PAINT to resolve nano-scale organization of seven targets simultaneously including synaptic proteins and cytoskeletal markers. These approaches demonstrated the broad applicability of fluorescence fluctuation spectroscopy and microscopy in resolving molecular dynamics and organization in cells.
Download or read book Fluorescence Fluctuation Spectroscopy FFS Part B written by and published by Academic Press. This book was released on 2012-12-29 with total page 395 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers fluorescence fluctuation spectroscopy - Contains chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells
Download or read book Fluorescence Spectroscopy and Microscopy written by Yves Engelborghs and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results. Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.
Download or read book Fluorescence Correlation Spectroscopy written by R. Rigler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book-length treatment of both the theoretical background to fluorescence correlation spectroscopy (FCS) and a variety of applications in various fields of science. The high spatial and temporal resolution of FCS has made it a powerful tool for the analysis of molecular interactions and kinetics, transport properties due to thermal motion, and flow. It contains an essential contribution from Nobel Prize winner M. Eigen, who is credited with inventing FCS.
Download or read book Optical Fluorescence Microscopy written by Alberto Diaspro and published by Springer Science & Business Media. This book was released on 2010-11-25 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.
Download or read book Handbook of Fluorescence Spectroscopy and Imaging written by Markus Sauer and published by John Wiley & Sons. This book was released on 2010-12-23 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.
Download or read book Fluorescence Spectroscopy and Microscopy in Biology written by Radek Šachl and published by Springer Nature. This book was released on 2023-04-27 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book is intended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.
Download or read book Fluorescence Correlation Spectroscopy written by R. Rigler and published by Springer. This book was released on 2011-10-04 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book-length treatment of both the theoretical background to fluorescence correlation spectroscopy (FCS) and a variety of applications in various fields of science. The high spatial and temporal resolution of FCS has made it a powerful tool for the analysis of molecular interactions and kinetics, transport properties due to thermal motion, and flow. It contains an essential contribution from Nobel Prize winner M. Eigen, who is credited with inventing FCS.
Download or read book Fluorescence Fluctuation Spectroscopy FFS written by Sergey Y. Tetin and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence Fluctuation Spectroscopy (FFS) is a growing family of methods for studying molecular motions and interactions. Introduced as Fluorescence Correlation Spectroscopy (FCS) 40 years ago, this elegant technique has evolved into a large group of powerful tools of modern fluorescence spectroscopy. FFS has found numerous applications in biophysical and biochemical studies of proteins, nucleic acids, and membranes. In combination with fluorescence imaging microscopy, FFS provides unique opportunities for characterizing dynamic processes in living cells and has been increasingly used by cell and systems biologists. Significant progress in the technology was achieved in the past decade, and the number of new FFS methods is still growing. The attraction of FFS relates to fact that it is based on statistical analysis of the fluctuations occurring at the molecular level. Therefore, these methods can resolve molecular populations and provide additional information about biological systems. The chapters in this two-volume publication are written by the authors and first-hand users of the methods under discussion. Part A is dedicated predominately to FFS methods. It contains well-established and also recently introduced experimental techniques and data analysis algorithms. Part B continues with FFS methods and also includes various FFS applications in vivo and in vitro. Here the reader will find specific examples and detailed experimental protocols developed for several biological systems. The purpose of these chapters is to show the power and potentials of the technology in biological research
Download or read book Fluorescence Fluctuation Spectroscopy Extended Beyound Diffusion written by Joseph Paul Skinner and published by . This book was released on 2007 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fluorescence Microscopy written by Ulrich Kubitscheck and published by John Wiley & Sons. This book was released on 2017-06-19 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.
Download or read book Fluorescence Microscopy written by Anda Cornea and published by Elsevier. This book was released on 2014-02-24 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. - Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research - Offers insight into common challenges in implementing techniques, as well as effective solutions
Download or read book Analysis and Applications of Fluorescence Fluctuation Spectroscopy written by Yan Chen and published by . This book was released on 1999 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nano scale Spatial and Temporal Fluorescence Fluctuations in Near field Microscopy Photobleaching Recovery and Non classical Elementary Reaction Kinetics written by Eric E. Monson and published by . This book was released on 1999 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book New Trends in Fluorescence Spectroscopy written by Bernard Valeur and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.
Download or read book Single Molecule Spectroscopy in Chemistry Physics and Biology written by Astrid Graslund and published by Springer Science & Business Media. This book was released on 2010-04-17 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the leading experts in the field, this book describes the development and current state of the art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.
