Download or read book Methods of Cell Separation written by P.T. Sharpe and published by Elsevier. This book was released on 1988-05-01 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments.A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for each method.Methods of Cell Separation is distinguished by three powerful assets: descriptions of the majority of cell separation methods currently being used; details of the experimental procedures involved in each method; and comparisons of the different methods for separating cell populations with particular reference to blood lymphocytes.An excellent addition to a distinguished series, and extremely useful as a laboratory manual.
Download or read book Dissection of T Cell Subsets and T Cell Functions with a Ficoll Velocity Sedimentation Gradient written by Harley Yau-Shuin Tse and published by . This book was released on 1977 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dendritic Cells in Fundamental and Clinical Immunology written by Eduard W. A. Kamperdijk and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 631 pages. Available in PDF, EPUB and Kindle. Book excerpt: These Proceedings contain the contributions of the participants of the Second International Symposium on Dendritic Cells that was held from the 1st to 25th of June 1992 in Amsterdam, the Netherlands. The First International Symposium on Dendritic Cells was organized as a Satellite symposium at the occasion of the 30th anniversary of the Japanese Reticuloendothelial Society by Dr. Y. Imai in Yamagata (Japan), in 1990. It was entitled "Dendritic Cells in Lymphoid Tissues," and focused primarily on the Interdigitating Cells (IDC), Epidermal Langerhans cells (LC) and Follicular Dendritic Cells (FDC) , from the point of view of human pathology. However, the concept of Dendritic Cell System, comprising the bone marrow derived IDC and LC but not the FDC, was based on animal experiments and mainly on in vitro experiments on isolated cells. In a report from the Reticuloendothelial Society Committee on Nomenclature in 1982, Tew, Thorbecke and Steinman had already characterized these different types of DC, but the gap between in vivo and in vitro function remained. In Amsterdam, the Symposium focused on the Role of Dendritic Cells in Fundamental and Clinical Immunology. First, recent developments in molecular biology of antigen presentation and cell biological aspects of signal transduction were discussed, in relation to the potential of DC to stimulate lymphocytes and to trigger their in vitro differentiation.
Download or read book Handbook of HLA Typing Techniques written by Kam M. Hui and published by CRC Press. This book was released on 1993-07-15 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of HLA Typing Techniques is an authoritative collection of HLA phenotyping and genotyping techniques to be used at the bench level and as a reference. The information presented, much of it previously unpublished, was written by leading authorities in the field of transplantation. Each chapter provides detailed methodologies, notes on the interpretation of tests, reference material, and appendices. Phenotyping topics covered include microlymphocytotoxicity assay, monoclonal antibodies, complement testing, serum screening for HLA antibodies, lymphocytotoxic crossmatching, and mixed and primed lymphocyte testing. Genotyping topics discussed include well-established RFLP analysis and polymerase chain reaction applications of PCR-SSO, PCR-RFLP, PCR-SSP, and PCR fingerprinting and related DNA heteroduplex technologies. Handbook of HLA Typing Techniques is an indispensable sourcebook for all researchers, practitioners, and students in the international tissue typing and human immunogenetics community.
Download or read book Cell Separation written by Thomas G. Pretlow and published by Academic Press. This book was released on 2014-05-10 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Separation: Methods and Selected Applications, Volume 4 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including liver cells, epidermal Langerhans cells, isolation of oval cells, clonal analysis, and the purification of polymorphonuclear leukocytes. Organized into 17 chapters, this volume begins with an overview of the central role of the liver in the metabolism of the body. This text then provides the analysis of Langerhans cells that allow modulation of their function and provide approaches to the treatment of skin disease. Other chapters consider the biological significance of oval cells. This book discusses as well the elucidation of the mechanisms of cellular proliferation, function, and differentiation in living tissues. The final chapter deals with the important applications of cell culture that involve continuous cell lines. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.
Download or read book Methods of Cell Separation written by Nicholas Catsimpoolas and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presently, the need for methods involving separation, identification, and characterization of different kinds of cells is amply realized among immu nologists, hematologists, cell biologists, clinical pathologists, and cancer researchers. Unless cells exhibiting different functions and stages of differ entiation are separated from one another, it will be exceedingly difficult to study some of the molecular mechanisms involved in cell recognition, specialization, interactions, cytotoxicity, and transformation. Clinical diag nosis of diseased states and use of isolated cells for therapeutic (e. g. , immunotherapy) or survival (e. g. , transfusion) purposes are some of the pressing areas where immediate practical benefits can be obtained by applying cell separation techniques. However. the development of such useful methods is still in its infancy. A number of good techniques exist based either on the physical or biological properties of the cells, and these have produced some valuable results. Still others are to be discovered. Therefore, the purpose of this open-end treatise is to acquaint the reader with some of the basic principles, instrumentation, and procedures pres ently in practice at various laboratories around the world and to present some typical applications of each technique to particular biological prob lems. To this end, I was fortunate to obtain the contribution of certain leading scientists in the field of cell separation, people who in their pioneer ing work have struggled with the particular problems involved in separating living cells and in some way have won.
Download or read book Immunology written by Werner Luttmann and published by Academic Press. This book was released on 2006-06-05 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunology was written for technical assistants, laboratory workers, students, doctoral candidates and for everyone interested in modern immunological methods.
Download or read book Applications of Chimeric Genes and Hybrid Proteins Part B Cell Biology and Physiology written by and published by Elsevier. This book was released on 2000-10-17 with total page 710 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Download or read book National Cancer Institute Monograph written by and published by . This book was released on 1973 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cytometry Part A written by and published by Elsevier. This book was released on 2000-10-31 with total page 683 pages. Available in PDF, EPUB and Kindle. Book excerpt: Each chapter presents a detailed background of the described method, its theoretical foundations, and its applicability to different biomedical material. Updated chapters describe either the most popular methods or those processes that have evolved the most since the past edition. Additionally, a large portion of the volume is devoted to clinical cytometry. Particular attention is paid to applications of cytometry in oncology, the most rapidly growing area. - Contains 56 extensive chapters authored by world authorities on cytometry - Covers a wide range of topics, including principles of cytometry and general methods, cell preparation, tandardization and quality assurance, cell proliferation, apoptosis, cell-cell/cell-environmental interactions, cytogenetics and molecular genetics, cell function and differentiation, experimental and clinical oncology, microorganisms, and infectious diseases - Describes in-depth the essential methods and scientific principles of flow and laser scanning cytometry and illustrates how they can be applied to the fields of biology and medicine - Complements the first and second editions on flow cytometry in the Methods in Cell Biology series and includes new sections on technology principles
Download or read book Mitochondria written by Liza A. Pon and published by Elsevier. This book was released on 2011-08-10 with total page 945 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an update on the step-by-step "how to" methods for the study mitochondrial structure, function, and biogenesis contained in the successful first edition. As in the previous edition, the biochemical, cell biological, and genetic approaches are presented along with sample results, interpretations, and pitfalls from each method.
Download or read book Aqueous Two Phase Systems written by Harry Walter and published by Elsevier. This book was released on 1994-04-18 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: General methodology and apparatus: phase diagrams, preparation and analysis of two-phase systems, partioning and affinity partitioning of macromolecules: Proteins, nucleic acids, studies on protein interactionsmolecular structure, charge, hydrophobicity, and conformational chan ges, partitioning and affinity partitioning of particulates,organellesseparation and subfractionation, menbrane: separation and subfractionation, membrane domain analysis, aqueous phase separation in biologicalsystems, aqueous two-phase systems in large-scale process biotechnolo gy, proteins; downstream processing, design of proteins for enhanced extraction, other applications of aqueous phases in biotechnology. Enzymology.
Download or read book The Impact of Food Bioactives on Health written by Kitty Verhoeckx and published by Springer. This book was released on 2015-04-29 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: “Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists...). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify the beneficial food components released in the gut during digestion; Support the effect of beneficial food components on human health; Promote harmonization of currently used digestion models Infogest meetings highlighted the need for a publication that would provide researchers with an insight into the advantages and disadvantages associated with the use of respective in vitro and ex vivo assays to evaluate the effects of foods and food bioactives on health. Such assays are particularly important in situations where a large number of foods/bioactives need to be screened rapidly and in a cost effective manner in order to ultimately identify lead foods/bioactives that can be the subject of in vivo assays. The book is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest and highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated and, as noted above, highlight the strengths and weaknesses of the various assays. It is also an important resource for undergraduate students in the ‘food and health’ arena.
Download or read book Plant Protoplasts and Genetic Engineering V written by Professor Dr. Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 1994-11-25 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: In continuation of Volumes 8, 9, 22, and 23, this new volume deals with the regeneration of plants from isolated protoplasts and genetic transformation in various species of Actinidia, Allocasuarina, Anthurium, Antirrhinum, Asparagus, Beta, Brassica, Carica, Casuarina, Cyphomandra, Eucalyptus, Ipomoea, Larix, Limonium, Liriodendron, Malus, Musa, Physcomitrella, Physalis, Picea, Rosa, Tagetes, Triticum, and Ulmus. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. The book contains a wealth of useful information for advanced students, teachers, and researchers in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology.
Download or read book Vertebrae Phototransduction and the Visual Cycle Part B written by and published by Elsevier. This book was released on 2000-04-19 with total page 887 pages. Available in PDF, EPUB and Kindle. Book excerpt: Major topics covered include photoreceptor proteins, phototransduction calcium-binding proteins and calcium measurement in photoreceptor cells, enzymes of the visual cycle, posttranslational and chemical modifications, analysis of animal models of retinal diseases. Inherited retinal disease; from the defective gene to its function and repair. This volume and its companion Volume 315 include newly developed methods to study vertebrate phototransduction and the visual cycle.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Download or read book Immunological Methods written by Ivan Lefkovits and published by Academic Press. This book was released on 2014-06-28 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunological Methods a compendium of basic research techniques being used in one of the largest immunology research institutes, the Basel Institute for Immunology, with particular emphasis given to new methodology. The procedures have been described by individuals judged to be highly expert in their specialties. In many instances the methods developed or adapted to unique uses by the contributors have not previously been described in detail. The book contains 34 chapters covering techniques for detection, isolation, and purification of antibodies (including dansylation, two-dimensional chromatography, isoelectric focusing, polyacrylamide gel electrophoresis, and isotachophoresis); measurement of equilibrium constants (equilibrium dialysis, filtration, and sedimentation); and isotope and fluorescent labeling and detection of cell-surface components. Techniques such as isotope laboratory maintenance; chemical modification of proteins, haptens, and solid supports, and haptenation of viable biological carriers; production of antisera against allotypes and histocompatibility antigens and production of antibody with clonai dominance; histocompatibility and MLR testing; and cell separation by haptenated gels and by velocity sedimentation of rosette-forming cells are also discussed. Other chapters cover detection of antibody-secreting and alloantigen-binding cells; immune responses in vitro and their analysis by limiting dilution; production of T-cell factors; hybridoma production by cell fusion; maintenance of cell lines and cloning in semisolid media; and the mathematical analysis of immunological data.
Download or read book Diagnostic Molecular Biology written by Chang-Hui Shen and published by Elsevier. This book was released on 2023-06-29 with total page 590 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner with each technique explained within its conceptual framework and current applications of clinical laboratory techniques comprehensively covered. This targeted approach covers the principles of molecular biology, including basic knowledge of nucleic acids, proteins and chromosomes; the basic techniques and instrumentations commonly used in the field of molecular biology, including detailed procedures and explanations; and the applications of the principles and techniques currently employed in the clinical laboratory. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq round out the discussion. Fully updated, this new edition adds recent advances in the detection of respiratory virus infections in humans, like influenza, RSV, hAdV, hRV but also corona. This book expands the discussion on NGS application and its role in future precision medicine. - Provides explanations on how techniques are used to diagnosis at the molecular level - Explains how to use information technology to communicate and assess results in the lab - Enhances our understanding of fundamental molecular biology and places techniques in context - Places protocols into context with practical applications - Includes extra chapters on respiratory viruses (Corona)
