Download or read book Expanded Bed Chromatography written by B. Mattiasson and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expanded bed adsorption chromatography is a novel processing technique for the purification of biomolecules, combining clarification, concentration and initial purification in one step. By such an integration it is possible to reduce the number of steps in the purification process, to shorten the processing time and to improve the yields. The technology is new, and interesting developments have taken place concerning the adsorbents, the processing technology and potential applications. Both small-scale laboratory processes and larger industrial processes are being developed. Expanded bed chromatography is one of the most exciting new developments in downstream processing in recent years. The technology will be a standard procedure when new biotechnological processes are being developed.

Download or read book Expanded Bed Chromatography written by B. Mattiasson and published by . This book was released on 2014-01-15 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Optimisation and Applications of Expanded Bed Adsorption Chromatography written by Peter Kumpalume and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Expanded Bed Adsorption Chromatography written by Norhafizah Abdullah and published by LAP Lambert Academic Publishing. This book was released on 2009 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Purifying protein is often a complicated task. This is due to protein s heterogeneity, complexity and instability when not in their native environment. It is the goal of this book to assemble the most generic approach for protein purification. The use of an expanded bed adsorption (EBA) chromatography as an integrative product capture and concentration technique allows single step purification of biomolecules from complex feedstock a possible alternative. There are a number of different approaches that can be taken in the choice and design of an appropriate adsorbent for use in EBA procedure. In this book, experimental investigation was focused on the choice of the ligand to be used on the solid phase adsorbent to ensure selective isolation of the target protein from a complex feedstock. True biospecific affinity, immobilized metal affinity chelating and ion exchange strategies were investigated for EBA purification. Recombinant glutathione-S-transferase (GST) expressed intracellularly in E.coli was chosen as a model protein. The book is aimed at researchers working in the area of protein purification.

Download or read book Expanded Bed Adsorption Chromatography for Protein Purification written by Janus Tingleff Søndergaard and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Improved Expanded Bed Adsorption Chromatography Systems written by Ayyoob Arpanael and published by . This book was released on 2008 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Protein Purification Using Expanded Bed Chromatography written by and published by . This book was released on 2003 with total page 86 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Radical Optimization of Expanded Bed Chromatography by the Implementation of an Interaction Energy Predictive Framework written by Prasad Babu Kakarla and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Scale Up and Optimization in Preparative Chromatography written by Anurag Rathore and published by CRC Press. This book was released on 2002-09-26 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text offers fundamental analysis of the underlying physicochemical principles to select optimal modes of interaction, design efficient separations, and increase productivity in the manufacture of novel therapeutics and pharmaceuticals.

Download or read book Advances in Expanded Bed Absorption Chromatography written by and published by . This book was released on 2009 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Comparison of Expanded bed and Packed bed Adsorption Systems written by Charles B. Hopkins and published by . This book was released on 1968 with total page 80 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Stem Cell Bioprocessing written by Tiago G. Fernandes and published by Elsevier. This book was released on 2013-11-15 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem cell bioprocessing describes the main large-scale bioprocessing strategies for both stem cell culture and purification, envisaging the application of these cells for regenerative medicine and drug screening. Bioreactor configurations are described, including their applications for stem cell expansion, and stem cell separation techniques such as isolation and purification are discussed. Basic definitions are provided concerning the different types of stem cells, from adult stem cells to the more recent induced pluripotent stem cells. The main characteristics of these different stem cell types are described, alongside the molecular mechanisms underlying their self-renewal and differentiation. The book also focuses on methodologies currently used for in vitro stem cell culture under static conditions, including the challenge of xeno-free culture conditions, as well as culture parameters that influence stem cell culture. Approaches for both stem cell culture and separation in micro-scale conditions are presented, including the use of cellular microarrays for high-throughput screening of the effect of both soluble and extracellular matrix molecules. A further section is dedicated to application of stem cells for regenerative medicine. - Maintains a unique focus on both the basic stem cell biology concepts, and their translation to large-scale bioprocessing approaches - Envisages the use of stem cells in regenerative medicine and drug screening applications - Discusses the application of microscale techniques as a tool to perform basic stem cell biology studies

Download or read book Molecular Biology and Biotechnology written by John M. Walker and published by Royal Society of Chemistry. This book was released on 2009 with total page 626 pages. Available in PDF, EPUB and Kindle. Book excerpt: This popular textbook has been completely revised and updated to provide a comprehensive overview and to reflect all the latest developments in this rapidly expanding area.

Download or read book Handbook of Bioseparations written by Satinder Ahuja and published by Elsevier. This book was released on 2000-06-23 with total page 740 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods, capillary electrophoresis, isoelectric focusing, and mass spectrometry. Among separation and purification methods, liquid-liquid distribution, displacement chromatography, expanded bed adsorption, membrane chromatography, and simulated moving bed chromatography are covered at length. Regulatory and economic considerations are addressed, as are plant and process equipment and engineering process control. A chapter on future developments highlights the application of DNA chip arrays as well as evolving methodologies for a large number of drugs that are under development for treatment of cancer, AIDS, rheumatoid arthritis, and Alzheimer's disease. Handbook of Bioseparations serves as an essential reference and guidebook for separation scientists working in the pharmaceutical and biotechnology industries, academia, and government laboratories.Key Features* Covers bioseparations of proteins, nucleic acids, and monoclonal antibodies* Encompasses both analytical and process-scale methods* Elucidates the importance of engineering process control* Details selection of plant and process equipment* Addresses economic considerations* Discusses future developments

Download or read book Handbook of Affinity Chromatography written by David S. Hage and published by CRC Press. This book was released on 2005-07-19 with total page 878 pages. Available in PDF, EPUB and Kindle. Book excerpt: This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry. The Handbook of Affinity Chromatograph

Download or read book Thin Layer Chromatography Revised And Expanded written by Bernard Fried and published by CRC Press. This book was released on 1999-01-04 with total page 475 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fourth edition of this work emphasizes the general practices and instrumentation involving TLC and HPTLC, as well as their applications based on compound types, while providing an understanding of the underlying theory necessary for optimizing these techniques. The book details up-to-date qualitative and quantitative densitometric experiments on organic dyes, lipids, antibiotics, pharmaceuticals, organic acids, insecticides, and more.

Download or read book Protein Purification written by Jan-Christer Janson and published by John Wiley & Sons. This book was released on 1998 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.