Download or read book Evaluation of Gas Chromatography mass Spectroscopy and Immunoassay Techniques for Assessing Polycyclic Aromatic Hydrocarbons in Creosote Contaminated Groundwater written by Claudia Isabel Rush and published by . This book was released on 2000 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods of Analysis by the U S Geological Survey National Water Quality Laboratory written by Mary C. Olson and published by . This book was released on 2004 with total page 62 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Determination of Polycyclic Aromatic Hydrocarbons by Gas Chromatography mass Spectrometry and Method Development in Supercritical Fluid Chromatography written by Jari Tuominen and published by . This book was released on 1990 with total page 77 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Polycyclic Aromatic Hydrocarbons in Lake Waters and Associated Sediments written by GW. Hodgson and published by . This book was released on 1975 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt: Marine and fresh water sediments exhibit a wide variety of polycyclic aromatic hydrocarbons. These range from the two-ringed biphenyl and naphthalene species to the seven-ringed coronene member, and include a variety of intermediate aromatics exhibiting isomeric differences as well as alkylated derivatives. Analytical methods were developed for this spectrum of aromatics involving extraction and purification techniques followed by a gas chromatographic separation combined with mass spectrometry for identification. In this manner, a total of 27 polycyclic aromatics were detected in Great Lakes sediments along with three aromatics and three dicarboxylic acid esters in waters of the Great Lakes. Limits of detection for aromatics were: 1 ng/liter in water, based on 30-liter water samples and 2 ng/g of dry sediment when extracted from 100 g of wet sediment.
Download or read book Using Gas Chromatography Mass Spectrometry for the Detection of Polycyclic Aromatic Hydrocarbons and Nitropolycyclic Aromatic Hydrocarbons in Soil and Diesel Particulate Matter written by Mario J. Mena (Jr) and published by . This book was released on 2018 with total page 65 pages. Available in PDF, EPUB and Kindle. Book excerpt: Particular matter (PM, e.g. soot, dust etc.) of a certain size is known to be hazardous to human health. These particles are small enough to travel through our respiratory system and induce respiratory illness by introducing foreign bodies and toxic chemicals to our systems. For instance, benzo(a)pyrene (BaP), is an example of a polycyclic aromatic hydrocarbon (PAH) frequently found in or on PM known to have carcinogenic and mutagenic effects. Polycyclic aromatic hydrocarbons are by-products of incomplete combustion such as vehicle emissions. Thus, when PM are airborne, they can interact with semi-volatile molecules such as PAH. In turn, the newly coupled system may penetrate our respiratory system. In addition to PAH, nitro polycyclic aromatic hydrocarbons (NPAHs, i.e. 1-nitro-pyrene) are being recognized by the International Agency for Research on Cancer as a Group 2a compound (probably carcinogenic to humans). This study aims to identify chemical signatures of forest fire and automobile emissions using ratios of PAH and its oxidants, including nitro-PAH, in a variety of media (e.g. soil, soot, PM). Nitro-PAH (NPAHs) are usually found in/on course/fine particular matter at concentrations on the order of 2ng/mL to 200ng/mL (Heidelore et al. 1991, Eiguren-Fernandez et al 2004). Therefore, a very sensitive technique is needed to detect NPAHs in various matrices representative of those found in the environment. This study will use the following PAH ratios to differentiate sources of incomplete combustion: B[a]P to (B[a]P+chrysene; pyrene to B[a]P; fluoranthrene to pyrene; and fluoranthrene to (fluoranthrene+pyrene). As for the NPAH:PAH ratios, the following will be used: 1-nitro-pyrene to pyrene; 6-nitro-chyresene to chrysene; and 6-nitro-benzo[a]pyrene to benzo[a]pyrene. Extractions of PAH will be performed using the accelerated solvent extraction technique. Gas chromatography-mass spectroscopy will be used to identify and quantify analytes. The results of this study may be used to inform citizens, city planners and lawmakers about the impacts of high-traffic events on human health.
Download or read book Investigations Into the Extraction and the Determination of Polycyclic Aromatic Hydrocarbons PAHs from Water by Solid phase Extraction and Capillary Gas Chromatography Mass Spectrometry written by Mohamed Elrutb and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Identification and Quantitation of Complex Polycyclic Aromatic Hydrocarbon Mixtures in Environmental Samples Using Comprehensive Two dimensional Gas Chromatography with Time of flight Mass Spectrometry written by Carlos Andres Manzano and published by . This book was released on 2012 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants and are mostly products of the incomplete combustion of organic material. PAHs are often found in environmental samples as a complex mixture of isomers. In addition, the same sources that produce complex PAH mixtures also produce other poorly characterized mixtures of organic compounds, commonly referred to as an unresolved complex mixture (UCM), that act as matrix interferences in the chromatographic analysis of samples. Conventional one-dimensional chromatographic techniques, such as gas chromatography coupled to mass spectrometry (GC/MS), are not sufficient for the analysis and quantitation of complex PAH mixtures present in environmental samples due to the high degree of overlap in compound vapor pressures, boiling points, and mass spectral fragmentation patterns. Therefore, the separation and quantitation of complex mixtures of individual PAH compounds in environmental samples requires high chromatographic resolution. Comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GCxGC/ToF-MS) was used for this study. GCxGC/ToF-MS uses two different gas chromatographic columns, with different separation mechanisms, for the analysis of complex environmental samples. In theory, the peak capacity in GCxGC/ToF-MS is equivalent to the product of the individual peak capacities of each column used. However, in practice, this is rarely obtained because of the existing correlation between the two GC columns used. This dissertation is a compilation of three studies related to analytical method development for the identification and quantitation of complex PAH mixtures (including parent-PAHs, alkyl-PAHs, oxy-PAHs, nitro-PAHs, thio-PAHs, chloro-PAHs, bromo-PAHs and PAHs with molecular weight higher than 300 Da) that may be present in environmental samples using novel column combinations in GCxGC/ToF-MS. The use of a liquid crystal column (LC-50) in the first dimension, followed by a nano-stationary phase column (NSP-35) in the second dimension, was evaluated for the separation of a standard PAH mixture containing 97 different PAHs. Two standard reference materials purchased from NIST (NIST SRM1650b - Diesel Particulate Matter and NIST SRM1975 - Diesel Extract) were used, after extraction and cleanup, for method validation and comparison between the commonly used non-polar x polar column combination and the LC-50 x NSP-35 column combination with high orthogonality. As part of the method validation, an aliquot of NIST SRM1975 (Diesel extract), without sample cleanup was also analyzed for PAHs, showing that the LC-50 x NSP-35 column combination was accurate (with an average absolute percent difference of approximately 30%) for the identification and quantitation of complex PAH mixtures in environmental samples, with reduced sample preparation prior to analysis. In addition, the LC-50 x NSP-35 column combination was used for the analysis of PAHs sorbed to polystyrene pellets deployed in an urban bay area as passive water samplers because one-dimensional GC/MS was ineffective due to the presence of a strong unresolved complex mixture (UCM) and matrix interferences.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1997 with total page 1860 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Advanced Techniques in Gas Chromatography Mass Spectrometry GC MS MS and GC TOF MS for Environmental Chemistry written by and published by Newnes. This book was released on 2013-09-26 with total page 525 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gas chromatography mass spectrometry (GC-MS) has been the technique of choice of analytical scientists for many years. The latest developments in instrumentation, including tandem mass spectrometry (MS-MS) and time-of-flight (TOF) detectors, have opened up and broadened the scope of environmental analytical chemistry. This book summarizes the major advances and relevant applications of GC-MS techniques over the last 10 years, with chapters by leading authors in the field of environmental chemistry. The authors are drawn from academia, industry and government. The book is organized in three main parts. Part I covers applications of basic GC-MS to solve environmental-related problems. Part II focuses on GC-MS-MS instrumentation for the analyses of a broad range of analysis in environmental samples (pesticides, persistent organic pollutants, endocrine disruptors, etc.). Part III covers the use of more advanced GC-MS techniques using low- and high-resolution mass spectrometry for many applications related to the environment, food and industry. Summarizes the major advances of GC-MS techniques in the last decade Presents relevant applications of GC-MS techniques Covers academic, industrial and governmental sectors
Download or read book Characterization of Waste Determination of Polycyclic Aromatic Hydrocarbons PAH in Waste Using Gas Chromatography Mass Spectrometry GC MS written by British Standards Institute Staff and published by . This book was released on 2009-03-31 with total page 40 pages. Available in PDF, EPUB and Kindle. Book excerpt: Wastes, Sludge, Soils, Soil pollution, Bitumens, Chemical analysis and testing, Determination of content, Aromatic hydrocarbons, Polynuclear aromatic hydrocarbons, Gas chromatography, Mass spectrometry, Extraction methods of analysis
Download or read book Novel Improvements on the Analytical Chemistry of Polycyclic Aromatic Hydrocarbons and Their Metabolites written by Wang Huiyong and published by . This book was released on 2010 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: Polycyclic aromatic hydrocarbons (PAH) are important environmental pollutants originating from a wide variety of natural and anthropogenic sources. Because many of them are highly suspect as etiological agents in human cancer, chemical analysis of PAH is of great environmental and toxicological importance. Current methodology for PAH follows the classical pattern of sample preparation and chromatographic analysis. Sample preparation pre-concentrates PAH, simplifies matrix composition, and facilitates analytical resolution in the chromatographic column. Among the several approaches that exist to pre-concentrate PAH from water samples, the Environmental Protection Agency (EPA) recommends the use of solid-phase extraction (SPE). High-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) are the basis for standard PAH identification and determination. Ultraviolet (UV) absorption and room temperature fluorescence detection are both widely used in HPLC, but the specificity of these detectors is modest. Since PAH identification is solely based on retention times, unambiguous PAH identification requires complete chromatographic resolution of sample components. When HPLC is applied to "unfamiliar" samples, the EPA recommends that a supporting analytical technique such as GC-MS be applied to verify compound identification and to check peak-purity HPLC fractions. Independent of the volume of extracted water, the approximate time required to separate and determine the sixteen "priority pollutants" (EPA-PAH) via HPLC is approximately 60min. If additional GC-MS analysis is required for unambiguous PAH determination, the total analysis time will reach 2-3 hours per sample. If the concentrations of target species are found to lie outside the detector's response range, the sample must be diluted and the process repeated. These are important considerations when routine analysis of numerous samples is contemplated. Parent PAH are relatively inert and need metabolic activation to express their carcinogenicity. By virtue of the rich heterogeneous distribution of metabolic products they produce, PAH provide a full spectrum of the complexity associated with understanding the initial phase of carcinogenesis. PAH metabolites include a variety of products such as expoxides, hydroxyl aromatics, quinines, dihydrodiols, dioepoxides, tetrols and water soluble conjugates. During the past decades tremendous efforts have been made to develop bio-analytical techniques that possess the selectivity and sensitivity for the problem at hand. Depending on the complexity of the sample and the relative concentrations of the targeted metabolites, a combination of sample preparation techniques is often necessary to reach the limits of detection of the instrumental method of analysis. The numerous preparation steps open ample opportunity to metabolite loss and collection of inaccurate data. Separation of metabolites has been accomplished via HPLC, capillary electrophoresis (CE) and GC-MS. Unfortunately, the existence of chemically related metabolic products with virtually identical fragmentation patterns often challenges the specificity of these techniques. This dissertation presents significant improvements in various fronts. Its first original component--which we have named solid-phase nano-extraction (SPNE)--deals with the use of gold nanoparticles (Au NPs) as extracting material for PAH. The advantages of SPNE are demonstrated for the analysis of PAH in water samples via both HPLC and Laser-Excited Time-Resolved Shpol'skii Spectroscopy (LETRSS). The same concept is then extended to the analysis of monohydroxy-PAH in urine samples via SPE- HPLC and In-Capillary SPNE-CE. The second original component of this dissertation describes the application of Shpol'skii Spectroscopy to the analysis of polar PAH metabolites. The outstanding selectivity and sensitivity for the direct analysis of PAH at trace concentration levels has made Shpol'skii spectroscopy a leading technique in environmental analysis. Unfortunately, the requirement of a specific guest-host combination--typically a non-polar PAH dissolved in an n-alkane-- has hindered its widespread application to the field of analytical chemistry. This dissertation takes the first steps in removing this limitation demonstrating its feasibility for the analysis of polar benzopyrene metabolites in alcohol matrixes.
Download or read book Characterization of waste Determination of polycyclic aromatic hydrocarbons PAH in waste using gas chromatography mass spectrometry GC MS written by and published by . This book was released on 2008 with total page 37 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Toxicological Profile for Polycyclic Aromatic Hydrocarbons written by and published by . This book was released on 1995 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Environmental Applications of Chromatography mass Spectrometry written by Wisam Abdulabbas Flayyih Al-Isawi and published by . This book was released on 2016 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS) are powerful analytical techniques that combine the separation power in a stationary phase with the detection power of mass spectrometry. GCMS and LC-MS have gained dominant positions in identifying and quantifying different analyte species due to their high selectivity, high sensitivity, and high reproducibility. My research utilizes the analytical power of LC-MS and GC-MS to address two environmental issues: 1) Identification of biodegradation products of the ionic liquid 1- butyl-3-methylimidazolium chloride (BMIMCl) by an activated sludge microbial community using LC-MS and 2) identification and quantification of polycyclic aromatic hydrocarbons (PAHs) in the Kalamazoo River oil spill using GC-MS. Three major metabolites from BMIMCl biodegradation were identified, and their structures were confirmed using MS/MS and 1H-NMR techniques. Many PAHs were identified, and their concentrations were quantified in the Kalamazoo River oil spill area using GC-MS.
Download or read book Development of Gas Chromatographic and Mass Spectrometric Techniques for the Analysis of Polycyclic Aromatic Compounds PACs in Environmental Samples written by IFEOLUWA GRACE. IDOWU and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concerns emanating from the presence of polycyclic aromatic compounds (PACs) in the environment especially as a yardstick for overall environmental health have been a challenge for several decades. Polycyclic aromatic compounds are a complex and structurally diverse class of compounds many of which can exist as isomers. Approaches to measure these compounds in environmental samples have relied on techniques based on gas chromatography coupled to mass spectrometry (GC/MS) at unit mass resolution. For example, the quantitation of the 16 US Environmental Protection Agency priority polycyclic aromatic hydrocarbons (PAHs) uses GC/MS and selected ion monitoring (SIM) of diagnostic m/z values of individual PAHs. Although this approach works well for the 16 priority PAHs, for more complex PACs, GC/MS in SIM mode is largely unreliable. Reasons for this include low selectivity of the SIM mode, insufficient chromatographic and mass resolution and the lack of commercially available authentic analytical standards. The overarching hypothesis of my thesis, therefore, is that more sophisticated chromatographic and MS techniques will provide more accurate measurements of PACs in the environment. The first advancement I made to the field of PAC research was the development of a one-dimensional GC coupled to MS method in the multiple reaction monitoring (MRM) to measure a suite of PACs. The method I developed was fully validated according to the EURACHEM guide - The Fitness for Purpose of Analytical methods and represents a significant improvement in the current SIM approach to quantitation of PACs in environmental samples. The second major advancement I made to the field of PAC research was the validation of a comprehensive 2D GC (GC×GC) high resolution time-of-flight (HR-TOF) MS method for the separation and quantitation of PACs. In this study I was able to exploit the increased peak capacity of the GC×GC system (relative to 1D) and the specificity of the HR-TOF/MS to quantify individual isomers of PAC compounds that were not attempted before. The methods I developed were paramount to the success of 2 other studies: (i) the GC/MS MRM method was used to understand the kinetics of PAH absorption to a highly sorptive medium that is used by industry to remediate the aquatic environment during/after an oil-spill and, (ii) the GC×GC-HR-TOF/MS was used in the detection of novel halogenated PACs in biological samples from the Alberta Oil Sands Region. The results of my work will have an impact and influence on future studies on source apportionment and chemical fingerprinting of crude-oil and will support much needed toxicological and monitoring studies of individual PAC isomers in the environment.
Download or read book Assessment of Dosimetry Requirements and Techniques for Measuring Polycyclic Aromatic Hydrocarbons 13 Refs written by and published by . This book was released on 1976 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: A prediction by ERDA is that, within a decade, 106 barrels/day of synthetic fuel will come from liquefaction of coal. The coproduction of highly carcinogenic polycyclic aromatic hydrocarbons (PAH) will necessitate much better dosimetry and means of personnel protection than exist today. Traditional techniques for measuring PAH are gas chromatography with mass spectroscopy, and fluorescence spectroscopy. Several newly developed, or developing techniques, may also lend themselves to PAH dosimetry. These include low-temperature Fourier transform infrared spectrometry, time-resolved fluorescence, room-temperature phosphorescence, portable mass spectrometry, and second derivative spectrometry. Special emphasis is given to potential use of the second derivative spectrometer for dosimetry purposes. Some of the advantages and limitations of these techniques for characterizing and measuring PAH under various conditions (vapor, liquid, solid, or aerosol) are discussed.
Download or read book Studies of Methods of Quantitation in Gas Chromatography mass Spectrometry and Its Applications to Quantitative Determination of Polycyclic Aromatic Hydrocarbons written by Aprajita Arora and published by . This book was released on 1994 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt:
