Download or read book Eosinophil Ultrastructure written by Rossana C.N. Melo and published by Academic Press. This book was released on 2023-01-05 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eosinophil Ultrastructure: Atlas of Eosinophil Cell Biology and Pathology entirely focuses on eosinophils and their functional roles in inflammation, host defense, and normal homeostatic activities. The book explores the ultrastructure of human eosinophils, highlighting biological processes observed under normal, experimental, and pathological conditions. Created to fill a void in the eosinophil literature, the book includes an extensive array of electron microscopic images that illustrate the diversity of eosinophil morphology. While the atlas is a learning and teaching tool, it is mainly a helpful resource for researchers to identify distinguishing features and structural changes that arise during studies of human eosinophils. The book also covers the ultrastructure of mouse eosinophils under normal and activation conditions and in the context of representative diseases. Gives guidelines to understand the human eosinophils in studies focused on structural biology, cellular immunology, innate and adaptive immunity, immune responses to pathogens, immunopathology, and inflammatory responses Provides a core of essential knowledge to identify both immature and mature eosinophils Comprises a representative compilation of the eosinophil ultrastructure during biological processes, such as activation and degranulation, mostly under experimental conditions Highlights eosinophil biological processes found in vivo during human diseases, thus providing a link between basic science and clinical aspects Helps identify distinguishing features and structural changes that arise during studies of human eosinophils after isolation from body fluids, while in cultures, or biopsies Explains the ultrastructural organization of mature and immature mouse eosinophils, highlighting the similarities/differences between them and human eosinophils
Download or read book Eosinophils in Health and Disease written by James J. Lee and published by Academic Press. This book was released on 2012-11-02 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eosinophils in Health and Disease provides immunology researchers and students with a comprehensive overview of current thought and cutting-edge eosinophil research, providing chapters on basic science, disease-specific issues, therapeutics, models for study and areas of emerging importance.
Download or read book Eosinophils written by Garry M. Walsh and published by Nova Science Publishers. This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The eosinophil is an enigmatic cell with a continuing ability to fascinate. In this book, experts in the field of eosinophil biology comprehensively update our knowledge on the human eosinophil in health and disease. Topics discussed include a synopsis of eosinophil characteristics, properties and role in disease. Important information on how eosinophils release their potent and toxic granule proteins will be covered and how these basic proteins give rise to pathologies including issues such as the function of the nerves.
Download or read book Functional Ultrastructure written by Margit Pavelka and published by Springer Science & Business Media. This book was released on 2005-12-05 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: The period between 1950 and 1980 were the golden It also provides unique insights into how pathological years of transmission electron microscopy and pro- processes affect cell organization. duced a plethora of new information on the structure of This information is vital to current work in which cells that was coupled to and followed by biochemical the emphasis is on integrating approaches from p- and functional studies. TEM was king and each micro- teomics, molecular biology, molecular imaging and graph of a new object produced new information that physiology, and pathology to understand cell functions led to new insights on cell and tissue organization and and derangements in disease. In this current era, there is their functions. The quality of data represented by the a growing tendency to substitute modern light mic- images of cells and tissues had been perfected to a very scopic techniques for electron microscopy because it is high level by the great microscopists of the era including less technically demanding and is more readily available Palade, Porter, Fawcett, Sjostrand, Rhodin and many to researchers. This atlas reminds us that the infor- others. At present, the images that we see in leading tion obtained by electron microscopy is invaluable and journals for the most part do not reach the same techni- has no substitute.
Download or read book Eosinophils in Health and Disease written by James J. Lee and published by Elsevier. This book was released on 2012-12-31 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written and edited by leaders in the field, Eosinophils in Health and Disease provides immunology researchers and students with a comprehensive overview of current thought and cutting-edge eosinophil research, providing chapters on basic science, disease-specific issues, therapeutics, models for study and areas of emerging importance. Despite an explosion of discovery in this area over the last decade, this is the only up-to-date resource on eosinophils and eosinophilia exists. This full-color volume provides an essential collection of world class information that is a must-have for any immunologist’s workbench or bookshelf. The only updated, comprehensive source of information on eosinophils on the market Written by over 80 leaders in the field Holistic view of the subject, including basic science, disease-specific chapters, therapeutic options and emerging areas of research
Download or read book Pathogenic Advances and Therapeutic Perspectives for Eosinophilic Inflammation written by Florence E. Roufosse and published by Frontiers Media SA. This book was released on 2018-12-06 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the recent approval of the first eosinophil-depleting therapeutic agents targeting the IL-5 pathway for treatment of severe eosinophilic asthma, eosinophils and eosinophilic disorders are in the limelight. Indeed, setbacks during clinical development of these compounds have revealed how much remains to be known about eosinophil biology in vivo, and have nurtured profuse research both on basic eosinophil biology and on pathogenic disease mechanisms, in order to better delineate the most meaningful targets for innovative therapeutic strategies. On one hand, variable degrees of eosinophil depletion observed in some compartments during IL-5-targeted treatment indicate that certain eosinophil subsets may not rely on this cytokine and/or that other important pro-eosinophilic mediators and signaling pathways are operative in vivo. On the other hand, it is increasingly clear that disorders involving eosinophils such as asthma are the final outcome of complex interactions between diverse cell types and mediators, beyond eosinophils and IL-5. These include type 2 helper T (Th2) cells and innate lymphoid cells, mast cells, and a variety of factors that either activate eosinophils or are released by them. Although a considerable amount of research has focused on asthma because it is a common condition and because management of severe asthma remains a major challenge, several rare eosinophilic disorders with more homogenous features have proven to be extremely useful models to reach a better understanding of the involvement of eosinophils in tissue damage and dysfunction, and of the micro-environmental interactions operating within the complex network of eosinophilic inflammation. Unraveling this interplay has resulted in advances in the development of molecular tools to detect disease subsets and to monitor therapeutic responses, and in identification of promising new therapeutic targets. This Research Topic dedicated to eosinophilic conditions covers aspects of the biology of eosinophils and closely related cells of particular relevance for drug development, reports on translational research investigating pathogenic mechanisms of specific eosinophilic disorders in humans that will likely result in significant changes in the way patients are managed, and presents an overview of the current advancement of targeted drug development for these conditions, with a special focus on asthma.
Download or read book Advances in Immunology written by and published by Academic Press. This book was released on 1986-10-16 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Immunology
Download or read book Ultrastructure of Mast Cells and Basophils written by Ann M. Dvorak and published by Karger Medical and Scientific Publishers. This book was released on 2005-01-01 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, the author reviews the ultrastructural studies of basophils and mast cells performed since the publication of an earlier monograph in 1991. These studies include the use of ultrastructural rules that allow identification of basophils and mast cells in new circumstances. Including more than 180 excellent micrographs, this book will be of interest to basic scientists in immunology, cell biology, secretion and angiogenesis research, and histochemistry. As mast cells and basophils play a crucial role in various diseases, it will be essential reading for allergists, immunologists, dermatologists, hematologists, gastroenterologists, rheumatologists, neurologists, pathologists, pulmonologists, and cardiologists.
Download or read book Megakaryocytes Platelets Macrophages and Eosinophils written by J. Robin Harris and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 363 pages. Available in PDF, EPUB and Kindle. Book excerpt: Blood Cell Biochemistry was initially conceived as part of the Plenum series Subcellular Biochemistry, from which it has developed into a separate series. The present volume is devoted primarily to contributions on megakaryocytes and platelets and, to a lesser extent, to macrophages and eosinophils. The book does not attempt a rigorous or total coverage of the particular topics; it represents the areas of current scientific activity and interest that were selected by the editor at the commencement of this project. In general, the approach has been similar to that adopted for Volume 1 of the series (Erythroid Cells); the same approach will be followed subsequently in Volume 3 (Lymphocytes and Granulocytes). This book opens with a developmentally oriented chapter by Janine Breton-Gorius on megakaryocyte maturation and platelet release in normal conditions, which serves to set the scene ultrastructurally for much of the data that follow. The biosynthesis and process ing of platelet glycoproteins in megakaryocytes is dealt with by Alain Duperray and his colleagues, and thereby provides an in-depth biochemical survey of the megakaryocyte. The applications and strengths of crossed immunoelectrophoresis for the study of platelet membrane proteins is then covered by Simon Karpatkin, and a detailed account of the heredity disorders of platelet function is provided by Francine Rendu and Evelyne Dupuy.
Download or read book A Tale of Two Interleukins how Il5 and Il33 Shapes Eosinophil Morphology and Inflammatory Potential written by Joshua Mitchell (Ph.D.) and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Activation of eosinophils by cytokines and chemokines is key step in the transition of eosinophils from the peripheral blood to tissue residency. While required for normal cellular function, increased abundance of activated eosinophils in tissues is a hallmark of eosinophilic diseases and disorders. We sought to elucidate further how two cytokines implicated in eosinophil function, IL5 and IL33, lead to eosinophil activation and how these activated states compare during initial cellular commitment, cellular polarization and migration, and downstream events like degranulation. We utilized proteomics, light microscopy, and cryo-electron microscopy tomography (cryoET) to illuminate mechanisms underlying cytokine activation of eosinophils. To investigate how eosinophils commit to activation when exposed to the cytokines IL5 and IL33 for 10 minutes, we used ten-plex tandem mass tagging to generate proteomic and phosphoproteomic datasets with which to compare effects of the cytokines on eosinophils purified from human peripheral blood. Three different datasets were generated: IL5-activated versus unactivated, IL33-activated versus unactivated, and IL5-activated versus IL33-activated. We found that in the first 10 minutes of exposure to cytokines, no significant proteomic changes are found, but the phosphoproteome changes pervasively. Phosphosites in proteins involved in basic cellular functions like cytoskeletal arrangement, vesicular trafficking, and splicing changed in the same direction comparing either cytokine to no treatment. However, different activation pathways were revealed comparing the two cytokines to each another, with IL5-activated eosinophils processing down a JAK-STAT cascade while IL33-activated eosinophils process through IL1-family signaling through a NFÎðB cascade. The result is positioning of RELA or phosphorylated STAT3 on nuclear speckles to initiate transcription and splicing of eosinophil cytokines/chemokines with the potential to change the phenotype of tissue-resident eosinophils in a cytokine-specific manner. Once activated, eosinophils undergo dramatic morphological changes. The naiÌ8ve unactivated sphere-like eosinophil rapidly morphs into adhesive acorn-like cells when exposed to IL5 and thin pancake-like cells when exposed to IL33. IL5-activated cells are more motile, whereas IL33-activated cells are more stationary. Live imaging shows that a dynamic microtubule network directs migration of the cells, with a stable population of microtubules maintain a stable network spanning across the nucleopod in the rear of the cell to the microtubule organizing center. Activated eosinophils are also substrate sensitive and show greater cellular death when activated on periostin versus fibrinogen. Granules of eosinophils contain high contents of toxic proteins, one of which, major basic protein-1, forms a crystalline core. Prior electron microscopy of eosinophils in tissues indicates that granule content can be released through fusion of the granule with the membrane and wholesale release (exocytosis), in parts (piecemeal degranulation), or deposition of granules after cell death (cytolysis). Lack of a method to address facilely how degranulation occurs within activated eosinophil populations on adhesive substrates has hindered how these mechanisms of release are related and coordinated. We established conditions in which we were able to plunge-frozen IL33-activated eosinophils for cryoET imaging without additional milling or sectioning. We observed numerous double-membrane vesicles that we presume are the same as so-called eosinophil sombrero vesicles noted in previous electron microscopic studies and were surprised to see naked crystalline cores of granules present intracellularly. These initial findings indicate that cryoET will yield important new insights into how activated eosinophils handle their granular content.
Download or read book Ultrastructural Pathology written by Norman F. Cheville and published by John Wiley & Sons. This book was released on 2009-06-30 with total page 997 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ultrastructural Pathology
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1989 with total page 1422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Human Eosinophils written by Gianni Marone and published by Karger Medical Scientific. This book was released on 2000 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eosinophils represent approximately 1% of peripheral blood leukocytes and play a central proinflammatory and immunoregulatory role in various immune disorders. This volume provides the reader with a comprehensive survey of the major aspects of human eosinophilic biology and immunology as well as the most clinically relevant aspects of eosinophil-related disorders. Expert contributions cover the ultrastructural and phenotypic characteristics of human eosinophils, and their fundamental biochemical features including receptors for IgE and chemokines. The production of cytokines and their role in the activation of eosinophils are reviewed. Furthermore, the classic eosinophil-related disorders such as hypereosinophilia, drug reactions, allergic disorders and some cardiovascular diseases are described. Each of the chapters is written by an investigator actively engaged in research on the topic under consideration. The well-edited and stimulating material compiled in this volume will be of interest to all those working in allergology, immunology, biochemistry and pulmonary medicine.
Download or read book Eosinophils written by Christopher J. F. Spry and published by Oxford University Press, USA. This book was released on 1988 with total page 524 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell biology and biochemical studies have shown how eosinophils respond to external stimuli, take part in inflammation, damage parasites, and often harm normal cells, especially in the heart. This book reviews exciting developments in eosinophil research, showing how new knowledge could be applied to current research and clinical practice. Over 2000 references are included, from a database of over 5000 used, forming the most thorough review of the literature currently available.
Download or read book Eosinophil Structure and Function written by Ruben Sher and published by . This book was released on 1979 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Severe Eosinophilic Disorders Mechanisms and Clinical Management written by Shigeharu Ueki and published by Frontiers Media SA. This book was released on 2019-12-04 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Eosinophils in Allergy and Inflammation written by Gerald J. Gleich and published by CRC Press. This book was released on 2019-08-16 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work presents the proceedings of a conference held at Adare Manor, County Limerick, Ireland. It provides an updated, in-depth review of the biological role of eosinophils in allergic diseases, summarizing basic knowledge of these unique cationic proteins. The book features an annotated discussion of the conference's post-presentation question-and-answer session.
