Download or read book Enzyme Regulation by Reversible Phosphorylation written by Philip Cohen and published by Elsevier Publishing Company. This book was released on 1984 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Recently Discovered Systems of Enzyme Regulation by Reversible Phosphorylation written by Philip Cohen and published by Elsevier-North-Holland Biomedical Press. This book was released on 1980 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Newly Discovered Systems of Enzyme Regulation by Reversible Phosphorylation written by The Biochemical Society and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Reversible Protein Phosphorylation in Cell Regulation written by R.L. Khandelwal and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book, published in association with the journal MOLECULAR AND CELLULAR BIOCHEMISTRY, is dedicated to Ed Krebs and Eddy Fischer in celebration of their 1992 Nobel Prize in Physiology and Medicine. Reversible protein phosphorylation is a research field pioneered and developed by Krebs and Fischer. This book contains short reviews and original research papers contributed by Krebs and Fischer's coworkers, both former and current. The contents reflect the two-way interaction between protein phosphorylation and other biomedical research fields. The chapters are grouped into four sections. The first two deal with structure/function aspects of protein kinases and protein mechanisms. Unlike many other research fields, which undergo periods of intense activity and productivity followed by relative calm, the protein phosphorylation field enjoyed continued growth both in scope and intensity, and the pace of this growth has increased markedly in recent years. This volume will provide a glimpse of the dynamism and diversity of the research activity representative of the current state of the field.
Download or read book Recently discovered Systems of enzyme regulation by riversible phosphorylation written by and published by . This book was released on 1980 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Mitochondria by Reversible Protein Phosphorylation written by and published by . This book was released on 2015 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mitochondria are dynamic organelles that must rapidly adapt to changing cellular metabolic and biosynthetic demands. Until recently, mitochondria have represented an underappreciated site of regulation by reversible phosphorylation. Furthermore, the pathogenic alterations that underlie metabolic disorders have increasingly represented one of the principal challenges in cell biology. Our work has focused on using quantitative proteomics and phosphoproteomics to identify dynamic phosphorylation sites and characterize the function of phosphorylation on mitochondrial enzymes. We began by charting the remodeling of the mouse liver mitochondrial proteome and phosphoproteome during both acute and chronic physiological transformations in more than 50 mice. Our analyses reveal that reversible phosphorylation is widespread in mitochondria, and is a key mechanism for regulating ketogenesis during the onset of obesity and type 2 diabetes. Specifically, we demonstrated that phosphorylation of a conserved serine on Hmgcs2 (S456) significantly enhances its catalytic activity in response to increased ketogenic demand. Additionally, we used activity based protein profiling to identify kinases that are enriched in mitochondria, as well as kinases that are differentially enriched in the mitochondria of obese mice. We further report on the change in oxygen consumption rates in isolated mitochondria in response to treatment with calcium, and show that calcium uptake is also dependent on electron transport. We also show that phosphorylation sites in isolated mitochondria can be rapidly modulated by acute (10min) treatment with calcium as a stimulus. Finally, we performed multiplexed quantitative mass spectrometry to identify the targets of the mitochondrial phosphatase PTC7. Using yeast deletion strains, we identify over 50 putative phosphorylation sites that are unique to a deletion of PTC7. Furthermore, we demonstrate that phosphorylation of a conserved serine on Hem15, the final step in heme biosynthesis, decreases enzyme activity. Collectively, our work describes the plasticity of this organelle at high resolution and provides a framework for investigating the roles of proteome restructuring and reversible phosphorylation in mitochondrial adaptation.
Download or read book The Hormonal Control of Gene Transcription written by P. Cohen and published by Elsevier. This book was released on 2012-12-02 with total page 517 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past few years there have been considerable advances in our understanding of cellular control mechanisms, and current research is now linking areas of biology that were previously thought of as being quite separate. Molecular Aspects of Cellular Regulation is a series of occasional books on multidisciplinary topics which illustrate general principles of cellular regulation. Previous volumes described Recently Discovered Systems of Enzyme Regulation by Reversible Phosphorylation (Volumes 1 and 3), The Molecular Actions of Toxins and Viruses (Volume 2), Molecular Mechanisms of Transmembrane Signalling (Volume 4) and Calmodulin (Volume 5). This sixth volume, The Hormonal Control of Gene Transcription, has now been published to highlight recent important advances in our understanding of this topic which is linking two of the most active areas of current biochemical and molecular biological research (hormone action and gene transcription) and leading to the emergence of unifying concepts.
Download or read book Regulation of Enzyme Activity written by James Henry Ottaway and published by Oxford University Press, USA. This book was released on 1988 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook presents the latest information on a topic vital to our understanding of metabolism. It concentrates on the ways that enzymes are reversibly regulated by ligand binding, covalent modification or other means. The text will provide a ready source of up-to-date information for undergraduate students.
Download or read book Biology of the NMDA Receptor written by Antonius M. VanDongen and published by CRC Press. This book was released on 2008-10-29 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: The NMDA receptor plays a critical role in the development of the central nervous system and in adult neuroplasticity, learning, and memory. Therefore, it is not surprising that this receptor has been widely studied. However, despite the importance of rhythms for the sustenance of life, this aspect of NMDAR function remains poorly studied. Written
Download or read book Cell to Cell Signalling written by A. Goldbeter and published by Academic Press. This book was released on 2014-06-28 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell to Cell Signalling: From Experiments to Theoretical Models is a collection of papers from a NATO Workshop conducted in Belgium in September 1988. The book discusses nerve cells and neural networks involved in signal transfers. The works of Hodgkin and Huxley presents a prototypic combination between experimental and theoretical approaches. The book discusses the coupling process found between secretory cells that modify their behavior. The text also analyzes morphogenesis and development, and then emphasizes the pattern formation found in Drosophila and in the amphibian embryo. The text also cite examples of immunological modeling that is related to the dynamics of immune networks based on idiotypic regulation. One paper analyzes the immune dynamism of HIV infection. The text notes that hormone signaling can be attributed as responsible for intercellular communication. Another paper examines how the dominant follicle in the ovarian cycle is selected, as well as the effectiveness of hormone secretion responsible for encoding the frequency of occurrence of periodic signals. The book also discusses heart signal sources such as cardiac dynamics and the response of periodically excited cardiac cells. The text can prove valuable for practioners in the field of neurology and cardiovascular medicine, and for researchers in molecular biology and molecular chemistry.
Download or read book Regulation of Phosphofructokinase by Reversible Inactivation written by Zhizhuang Zhao and published by . This book was released on 1990 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: The interaction of skeletal muscle phosphofructokinase with a variety of acidic proteins including calmodulin and troponin C and with nucleic acids (RNA and DNA) is manifest in a reversible, time-dependent loss of catalytic activity. The inactivation is affected by a number of factors. Substrates (fructose-6-phosphate and ATP), positive effectors (fructose-2,6-bisphosphate, ADP, AMP, and inorganic phosphate), and a negative effector (citrate) of phosphofructokinase all diminish inactivation while a decrease in pH or an increase in temperature favor the inactivation process. Proteins which bind calmodulin or troponin C -- such as troponin I, melittin, and protamine -- reduce the inactivation, possibly by competing with the enzyme. The inactivation can be reversed by the addition of fructose-2,6-bisphosphate, ATP, or ADP. Studies with specific proteolytic fragments of troponin C indicate that the residues near calcium-binding site III are involved in the inactivation of phosphofructokinase. A general kinetic model for the inactivation has been developed mathematically and applied to the data. A novel electrophoresis procedure demonstrates directly the specific interaction of phosphofructokinase with calmodulin or troponin C. The specific calcium-dependent association of phosphofructokinase with calmodulin or troponin C is manifest in large increases in the rate of and extent of in vitro phosphorylation of the enzyme catalyzed by cAMP-dependent protein kinase. Two distinct phosphorylation sites in phosphofructokinase have been identified. The first, corresponding to the known C-terminal site -- which has the sequence His-Ile-Ser-Arg-Lys-Arg-Ser(P)-Gly-Glu -- is demonstrated both in the control and in enzyme samples which have been phosphorylated in the presence of calmodulin or troponin C. The second is a novel site found in the middle of the phosphofructokinase molecule with the sequence Lys- Leu-Arg-Gly-Arg-Ser(P)-Phe-Met. Phosphorylation at this site is induced by calmodulin or troponin C in the presence of calcium. Preliminary studies indicate that phosphorylation has little effect on the activity of phosphofructokinase as determined by standard assays on samples taken from the phosphorylation mixtures.
Download or read book Co and Post Translational Modifications of Therapeutic Antibodies and Proteins written by T. Shantha Raju and published by John Wiley & Sons. This book was released on 2019-03-15 with total page 293 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Comprehensive Guide to Crucial Attributes of Therapeutic Proteins in Biological Pharmaceuticals With this book, Dr. Raju offers a valuable resource for professionals involved in research and development of biopharmaceutical and biosimilar drugs. This is a highly relevant work, as medical practitioners have increasingly turned to biopharmaceutical medicines in their search for safe and reliable treatments for complex diseases, while pharmaceutical researchers seek to expand the availability of biopharmaceuticals and create more affordable biosimilar alternatives. Readers receive a thorough overview of the major co-translational modifications (CTMs) and post-translational modifications (PTMs) of therapeutic proteins relevant to the development of biotherapeutics. The majority of chapters detail individual CTMs and PTMs that may affect the physicochemical, biochemical, biological, pharmacokinetic, immunological, toxicological etc. properties of proteins. In addition, readers are guided on the methodology necessary to analyze and characterize these modifications. Thus, readers gain not only an understanding of CTMs/PTMs, but also the ability to design and assess their own structure-function studies for experimental molecules. Specific features and topics include: Discussion of the research behind and expansion of biopharmaceuticals Twenty chapters detailing relevant CTMs and PTMs of proteins, such as glycosylation, oxidation, phosphorylation, methylation, proteolysis, etc. Each chapter offers an introduction and guide to the mechanisms and biological significance of an individual CTM or PTM, including practical guidance for experiment design and analysis An appendix of biologic pharmaceuticals currently on the market, along with an assessment of their PTMs and overall safety and efficacy This volume will prove a key reference on the shelves of industry and academic researchers involved in the study and development of biochemistry, molecular biology, biopharmaceuticals and proteins in medicine, particularly as biopharmaceuticals and biosimilars become ever more prominent tools in the field of healthcare.
Download or read book Regulation of Hormone sensitive Lipase Through Reversible Phosphorylation written by Peter Strålfors and published by . This book was released on 1983 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dyneins written by Stephen M. King and published by Academic Press. This book was released on 2011-08-11 with total page 657 pages. Available in PDF, EPUB and Kindle. Book excerpt: Research on dyneins has a direct impact on human diseases, such as viruses and cancer. With an accompanying website showing over 100 streaming videos of cell dynamic behavior for best comprehension of material, Dynein: Structure, Biology and Disease is the only reference covering the structure, biology and application of dynein research to human disease. From bench to bedside, Dynein: Structure, Biology and Disease offers research on fundamental cellular processes to researchers and clinicians across developmental biology, cell biology, molecular biology, biophysics, biomedicine, genetics and medicine. Broad-based up-to-date resource for the dynein class of molecular motors Chapters written by world experts in their topics Numerous well-illustrated figures and tables included to complement the text, imparting comprehensive information on dynein composition, interactions, and other fundamental features
Download or read book Principles and Applications of Molecular Diagnostics written by Nader Rifai and published by Elsevier. This book was released on 2018-06-13 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles and Applications of Molecular Diagnostics serves as a comprehensive guide for clinical laboratory professionals applying molecular technology to clinical diagnosis. The first half of the book covers principles and analytical concepts in molecular diagnostics such as genomes and variants, nucleic acids isolation and amplification methods, and measurement techniques, circulating tumor cells, and plasma DNA; the second half presents clinical applications of molecular diagnostics in genetic disease, infectious disease, hematopoietic malignancies, solid tumors, prenatal diagnosis, pharmacogenetics, and identity testing. A thorough yet succinct guide to using molecular testing technology, Principles and Applications of Molecular Diagnostics is an essential resource for laboratory professionals, biologists, chemists, pharmaceutical and biotech researchers, and manufacturers of molecular diagnostics kits and instruments. Explains the principles and tools of molecular biology Describes standard and state-of-the-art molecular techniques for obtaining qualitative and quantitative results Provides a detailed description of current molecular applications used to solve diagnostics tasks
Download or read book The Enzymes written by Paul D. Boyer and published by . This book was released on 1970 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: Conteúdo: Structure and control.
Download or read book Protein Phosphatases written by P. Agostinis and published by . This book was released on 1988-07 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt:
