Download or read book Engineering Self assembling Living Structures with Mammalian Synthetic Biology written by Jessica Jane Tordoff and published by . This book was released on 2020 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: The shapes and structures created by living organisms have properties that any engineer would desire: they are self-healing, growing, adaptive, and range in complexity and material property from the strength of bone to the lightweight flexibility of an insect wing. The goal of this thesis is to use synthetic biology to design, control, and understand the range of structures that can be constructed using a ubiquitous tool for self-organization in animal development: cell sorting. We first use experiments and computational modeling to demonstrate how incompletely sorted structures can be systematically designed by quantitative control of cell composition. By varying the number of highly adhesive and less adhesive cells in multicellular aggregates, we find that cell type ratio and total number of cells are controllers of pattern formation, and the resulting structures are maintained over the course of days. Next, we establish a set of design rules for cell sorting-driven shape assembly using cell lines with engineered genetic circuits that can induce expression of different cadherins. We show that, even when well mixed, populations of cells with different cadherin expression profiles sort themselves in predictable ways. The resulting shapes vary significantly, including planes of semi-regular polka dots, a sphere engulfed by an outer shell, maze-like intertwined populations, and radial protrusions from a core. We can reliably select between these shapes and control their properties by changing induction of cadherin expression, population ratio, cadherin identity, and total aggregate size. Finally, we have designed and constructed a recombinase-based tool to break symmetry in a population of cells, with the ultimate goal of controlling the autonomous creation of different subpopulations from a single cell. By creating a platform to programmably generate multicellular forms, this thesis aims to establish design principles for synthetic morphogenesis and provide a framework to control living shapes.
Download or read book Engineered Living Materials written by Wil V. Srubar III and published by Springer Nature. This book was released on 2022-02-16 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will serve as a primer for readers to understand recent advances, applications, and current challenges in the field of Engineered Living Materials. The chapters cover core science and engineering research areas, including (1) advances in synthetic biology and genetic programmability for Engineered Living Materials, (2) functional Engineered Living Material for application in energy, electronics, and construction, and (3) novel manufacturing approaches for Engineered Living Materials at multiple scales. The emerging field of Engineered Living Materials represents a significant paradigm shift in materials design and synthesis, in which living cells are used to impart biologically active functionalities to manmade materials. The result is a genetically programmable augmentation of non-living matter to exhibit unprecedented life-like (i.e., living) capabilities. At the intersection of synthetic biology and materials science, the field of Engineered Living Materials exhibits unprecedented promise and potential to alter the way we synthesize new materials and design medical devices, fabrics, robotics, commodity polymers, and construction materials. Materials with attributes of living systems can be engineered with an ability to respond to their environment and designed to self-repair in response to physical or other stresses or detect the presence of specific stimuli, such as light, heat, pressure, or hazardous chemical compounds. Although nascent, scientists and researchers in the field of Engineered Living Materials have made marked advances in demonstrating a potential to revolutionize a multitude of science and engineering disciplines. This volume will define the current state of the art of Engineered Living Materials, and highlight grand opportunities and challenges that abound at the nexus of synthetic biology and materials science and engineering.
Download or read book Lessons on Synthetic Bioarchitectures written by Eva-Kathrin Ehmoser-Sinner and published by Springer. This book was released on 2018-03-09 with total page 70 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook discusses the new relationship between artificial, synthetic material and living matter, and presents defined examples of approaches aiming for the creation of artificial cells. It also offers insights into the world of synthetic biology from its origins to the present day, showing what is currently possible in this discipline. Furthermore, it examines the ethical concerns and potential threats posed by this new field. The textbook is based on a lecture of the same title, held for master’s students at the University of Natural Resources and Life Sciences (BOKU), Vienna, and is primarily intended for students of synthetic biology, biotechnology and bioengineering. It is also of interest to research scientists from other disciplines wishing to learn more about the state of the art of synthetic biology and its future.
Download or read book Self assembling Biomaterials written by Helena S. Azevedo and published by Woodhead Publishing. This book was released on 2018-04-17 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Self-assembling biomaterials: molecular design, characterization and application in biology and medicine provides a comprehensive coverage on an emerging area of biomaterials science, spanning from conceptual designs to advanced characterization tools and applications of self-assembling biomaterials, and compiling the recent developments in the field. Molecular self-assembly, the autonomous organization of molecules, is ubiquitous in living organisms and intrinsic to biological structures and function. Not surprisingly, the exciting field of engineering artificial self-assembling biomaterials often finds inspiration in Biology. More important, materials that self-assemble speak the language of life and can be designed to seamlessly integrate with the biological environment, offering unique engineering opportunities in bionanotechnology. The book is divided in five parts, comprising design of molecular building blocks for self-assembly; exclusive features of self-assembling biomaterials; specific methods and techniques to predict, investigate and characterize self-assembly and formed assemblies; different approaches for controlling self-assembly across multiple length scales and the nano/micro/macroscopic properties of biomaterials; diverse range of applications in biomedicine, including drug delivery, theranostics, cell culture and tissue regeneration. Written by researchers working in self-assembling biomaterials, it addresses a specific need within the Biomaterials scientific community. Explores both theoretical and practical aspects of self-assembly in biomaterials Includes a dedicated section on characterization techniques, specific for self-assembling biomaterials Examines the use of dynamic self-assembling biomaterials
Download or read book Synthetic Biology written by Luc Brunsveld and published by Royal Society of Chemistry. This book was released on 2017-11-29 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading researchers draw on the recent literature in Synthetic Biology, from both dedicated journals and broader sources, making this an essential reference to any library supporting this research in this emerging field.
Download or read book New Frontiers and Applications of Synthetic Biology written by Vijai Singh and published by Academic Press. This book was released on 2022-01-12 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: New Frontiers and Applications of Synthetic Biology presents a collection of chapters from eminent synthetic biologists across the globe who have established experience and expertise working with synthetic biology. This book offers several important areas of synthetic biology which allow us to read and understand easily. It covers the introduction of synthetic biology and design of promoter, new DNA synthesis and sequencing technology, genome assembly, minimal cells, small synthetic RNA, directed evolution, protein engineering, computational tools, de novo synthesis, phage engineering, a sensor for microorganisms, next-generation diagnostic tools, CRISPR-Cas systems, and more. This book is a good source for not only researchers in designing synthetic biology, but also for researchers, students, synthetic biologists, metabolic engineers, genome engineers, clinicians, industrialists, stakeholders and policymakers interested in harnessing the potential of synthetic biology in many areas. Offers basic understanding and knowledge in several aspects of synthetic biology Covers state-of-the-art tools and technologies of synthetic biology, including promoter design, DNA synthesis, DNA sequencing, genome design, directed evolution, protein engineering, computational tools, phage design, CRISPR-Cas systems, and more Discusses the applications of synthetic biology for smart drugs, vaccines, therapeutics, drug discovery, self-assembled materials, cell free systems, microfluidics, and more
Download or read book Protein based Engineered Nanostructures written by Aitziber L. Cortajarena and published by Springer. This book was released on 2016-09-27 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is devoted to the engineering of protein-based nanostructures and nanomaterials. One key challenge in nanobiotechnology is to be able to exploit the natural repertoire of protein structures and functions to build materials with defined properties at the nanoscale using “bottom-up” strategies. This book addresses in an integrated manner all the critical aspects that need to be understood and considered to design the next generation of nano-bio assemblies. The book covers first the fundamentals of the design and features of the protein building blocks and their self-assembly illustrating some of the most relevant examples of nanostructural design. Finally, the book contains a section dedicated to demonstrated applications of these novel bioinspired nanostructures in different fields from hybrid nanomaterials to regenerative medicine. This book provides a comprehensive updated review of this rapidly evolving field.
Download or read book Synthetic Biology A Very Short Introduction written by Jamie A. Davies and published by Oxford University Press. This book was released on 2018-07-19 with total page 145 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthetic biology is one of the 21st century's fastest growing fields of research, as important for technology as for basic science. Building on traditional genetic engineering, which was restricted to changing one or two genes, synthetic biology uses multi-gene modules and pathways to make very significant changes to what cells can do. Synthetic biologists aim to have an impact in fields as diverse as drug manufacture, biofuel production, tackling pollution, and medical diagnostics. Further ahead, synthetic biology may even make possible the long-standing goal of creating new life from non-living starting materials. This Very Short Introduction provides a concise explanation of what synthetic biology is, and how it is beginning to affect many fields of technology. Jamie Davies also discusses the considerable controversies surrounding synthetic biology, from questions over the assumption that engineering concepts can be applied to living systems easily, to scepticism over the claims for commercial promise, fears that the dangers of engineering life are worse than its benefits, and concerns over whether humans should be designing living systems at all. ABOUT THE SERIES: The Very Short Introductions series from Oxford University Press contains hundreds of titles in almost every subject area. These pocket-sized books are the perfect way to get ahead in a new subject quickly. Our expert authors combine facts, analysis, perspective, new ideas, and enthusiasm to make interesting and challenging topics highly readable.
Download or read book Mammalian Synthetic Biology written by Jamie Davies and published by . This book was released on 2019-12-12 with total page 137 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written primarily for mid-to-upper level undergraduates, this primer will introduce students to topics at the forefront of the subject that are being applied to probe biological problems, or to address the most pressing issues facing society. These topics will include those that form thecornerstone of contemporary research, helping students to make the transition to active researcher.This primer introduces the challenges and opportunities of applying synthetic biological techniques to mammalian cells, tissues, and organisms. It covers the special features that make engineering mammalian systems different from engineering bacteria, fungi, and plants, and provides an overview ofcurrent techniques. A variety of cutting-edge examples illustrate the different purposes of mammalian synthetic biology, including pure biomedical research, drug production, tissue engineering, and regenerative medicine.
Download or read book The Science and Applications of Synthetic and Systems Biology written by Institute of Medicine and published by National Academies Press. This book was released on 2011-12-30 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many potential applications of synthetic and systems biology are relevant to the challenges associated with the detection, surveillance, and responses to emerging and re-emerging infectious diseases. On March 14 and 15, 2011, the Institute of Medicine's (IOM's) Forum on Microbial Threats convened a public workshop in Washington, DC, to explore the current state of the science of synthetic biology, including its dependency on systems biology; discussed the different approaches that scientists are taking to engineer, or reengineer, biological systems; and discussed how the tools and approaches of synthetic and systems biology were being applied to mitigate the risks associated with emerging infectious diseases. The Science and Applications of Synthetic and Systems Biology is organized into sections as a topic-by-topic distillation of the presentations and discussions that took place at the workshop. Its purpose is to present information from relevant experience, to delineate a range of pivotal issues and their respective challenges, and to offer differing perspectives on the topic as discussed and described by the workshop participants. This report also includes a collection of individually authored papers and commentary.
Download or read book Cell Free Synthetic Biology written by Jian Li and published by Frontiers Media SA. This book was released on 2022-01-13 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Foundational Platform for Mammalian Synthetic Biology written by Noah Justin Davidsohn and published by . This book was released on 2013 with total page 129 pages. Available in PDF, EPUB and Kindle. Book excerpt: The emergent field of synthetic biology is different from many other biological engineering efforts, in that its roots, design principles, and forward engineering perspective have been adopted from electrical engineering and computer science. Synthetic biology is uniquely poised to make great contributions to numerous fields such as bio-fuel, energy production, agriculture and eco-remediation, national defense, and biomedical and tissue engineering. Considerable progress has been made in engineering novel genetic circuits in many different organisms. However, not much progress has been made toward developing a formal methodology to engineer complex genetic systems in mammalian cells. One of the most promising areas of research is the study of embryonic and adult stem cells. Synthetic biology has the potential to greatly impact the progression and development of research in this area of study. A critical impediment to the development of stem cell engineering is the innate complexity, little to no characterization of parts, and limited compositional predictive capabilities. In this thesis, I discuss the strategies used for constructing and optimizing the performance of signaling pathways, the development of a large mammalian genetic part and circuit library, and the characterization and implementation of novel genetic parts and components aimed at developing a foundation for mammalian synthetic biology. I have designed and tested several orthogonal strategies aimed at cell-cell communication in mammalian cells. I have designed a characterization framework for the complete and proper characterization of genetic parts that allows for modular predictive composition of genetic circuits. With this characterization framework I have generated a small library of characterized parts and composite circuits that have well defined input-output relationships that can be used in novel genetic architectures. I also aided in the development of novel analysis and computational tools necessary for accurate predictive composition of these novel circuits. This work collectively provides a foundation for engineering complex intracellular transcriptional networks and intercellular signaling systems in mammalian cells.
Download or read book Morphogenetic Engineering written by René Doursat and published by Springer. This book was released on 2012-12-13 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Generally, spontaneous pattern formation phenomena are random and repetitive, whereas elaborate devices are the deterministic product of human design. Yet, biological organisms and collective insect constructions are exceptional examples of complex systems that are both self-organized and architectural. This book is the first initiative of its kind toward establishing a new field of research, Morphogenetic Engineering, to explore the modeling and implementation of “self-architecturing” systems. Particular emphasis is placed on the programmability and computational abilities of self-organization, properties that are often underappreciated in complex systems science—while, conversely, the benefits of self-organization are often underappreciated in engineering methodologies. Altogether, the aim of this work is to provide a framework for and examples of a larger class of “self-architecturing” systems, while addressing fundamental questions such as br” How do biological organisms carry out morphogenetic tasks so reliably? br” Can we extrapolate their self-formation capabilities to engineered systems?br” Can physical systems be endowed with information (or informational systems be embedded in physics) so as to create autonomous morphologies and functions?br” What are the core principles and best practices for the design and engineering of such morphogenetic systems?
Download or read book Structural DNA Nanotechnology written by Nadrian C. Seeman and published by Cambridge University Press. This book was released on 2015 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the founder of the field, this is a comprehensive and accessible introduction to structural DNA nanotechnology.
Download or read book Biodefense in the Age of Synthetic Biology written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2019-01-05 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientific advances over the past several decades have accelerated the ability to engineer existing organisms and to potentially create novel ones not found in nature. Synthetic biology, which collectively refers to concepts, approaches, and tools that enable the modification or creation of biological organisms, is being pursued overwhelmingly for beneficial purposes ranging from reducing the burden of disease to improving agricultural yields to remediating pollution. Although the contributions synthetic biology can make in these and other areas hold great promise, it is also possible to imagine malicious uses that could threaten U.S. citizens and military personnel. Making informed decisions about how to address such concerns requires a realistic assessment of the capabilities that could be misused. Biodefense in the Age of Synthetic Biology explores and envisions potential misuses of synthetic biology. This report develops a framework to guide an assessment of the security concerns related to advances in synthetic biology, assesses the levels of concern warranted for such advances, and identifies options that could help mitigate those concerns.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Materials Nanoarchitectonics written by Katsuhiko Ariga and published by Elsevier. This book was released on 2023-12-15 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: Materials Nanoarchitectonics: From Integrated Molecular Systems to Advanced Devices provides the latest information on the design and molecular manipulation of self-organized hierarchically structured systems using tailor-made nanoscale materials as structural and functional units. The book is organized into three main sections that focus on molecular design of building blocks and hybrid materials, formation of nanostructures, and applications and devices. Bringing together emerging materials, synthetic aspects, nanostructure strategies, and applications, the book aims to support further progress, by offering different perspectives and a strong interdisciplinary approach to this rapidly growing area of innovation. This is an extremely valuable resource for researchers, advanced students, and scientists in industry, with an interest in nanoarchitectonics, nanostructures, and nanomaterials, or across the areas of nanotechnology, chemistry, surface science, polymer science, electrical engineering, physics, chemical engineering, and materials science. Offers a nanoarchitectonic perspective on emerging fields, such as metal-organic frameworks, porous polymer materials, or biomimetic nanostructures Discusses different approaches to utilizing "soft chemistry" as a source for hierarchically organized materials Offers an interdisciplinary approach to the design and construction of integrated chemical nano systems Discusses novel approaches towards the creation of complex multiscale architectures
