Download or read book Engineering Protein Molecular Recognition written by Melissa Geddie and published by . This book was released on 2007 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book MOLECULAR RECOGNITION BIOTECHNOLOGY CHEMICAL ENGINEERING AND MATERIALS APPLICATIONS written by JASON A. MCEVOY and published by Lulu.com. This book was released on 2011 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Engineering written by Raghupathy Sarma and published by Elsevier. This book was released on 2012-12-02 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Engineering: Applications in Science, Medicine, and Industry deals with the scientific, medical, and industrial applications of protein engineering. Topics range from protein structure and design to mutant analysis and complex systems. Applications such as production of novel antibiotics, genetic transformation of plants, and genetic engineering of bioinsecticides are described. This book is comprised of 25 chapters and begins with an overview of trends and developments in protein chemistry and their relevance to protein engineering, followed by a discussion on protein sequence data banks. Subsequent chapters explore the design and construction of biologically active peptides, including hormones; structural and functional analysis of thermophile proteins; the conformation of diphtheria toxin; and applications of surface-simulation synthesis in protein molecular recognition. The use of oligonucleotide-directed site-specific mutagenesis in functional analysis of the signal peptide for protein secretion is also considered. The results of studies on the mechanism of membrane fusion are presented. This monograph will serve as a useful guide for those who are already working on protein engineering and those who are about to start research in this field.
Download or read book Design of Functional Proteins for Molecular Recognition written by Allison Jeannette Doerr and published by . This book was released on 2005 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Combinatorial Protein Engineering Applied to Enzyme Catalysis and Molecular Recognition written by Malin Eklund and published by . This book was released on 2004 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Peptide And Protein Engineering For Biotechnological And Therapeutic Applications written by Pierre Rousselot-pailley and published by World Scientific. This book was released on 2023-01-06 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptides and proteins are crucial biomolecules in life. The manifold functions they carry out range from molecular recognition and signaling to catalysis and immune response. However, the native systems are limited to a reduced toolbox of chemical functionalities as well as tridimensional structures. Widening these toolboxes could pave the way to engineer peptides and proteins with enhanced properties compared to their native counterparts and/or with structures and functions unprecedented in Nature. Advances in the chemical and biological synthesis of peptides and proteins, in computational tools, in molecular biology and in high-throughput screening methods are making this realm possible.This book aims to give an overview of the last developments in the field of peptide and protein engineering. It comprises a collection of chapters that span from the production of simple non-proteinogenic building blocks and peptidic scaffolds of different sizes and structures to more complex systems including peptide-based nanomaterials, enzymes and artificial metalloenzymes. Different strategies are described where chemical and biological tools have been developed and combined to attain the desired properties and sought functionalities.The diverse systems described in this book highlight the progress in this important field and represent the starting points for the development of functional biomolecules, biomaterials and hybrid systems capable of addressing key societal challenges of our times in relevant areas such health, environment and energy.
Download or read book Engineering OB fold Protein Domains as Versatile Scaffolds for Molecular Recognition written by Vickery Arcus and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: OB-fold protein domains are found in all three kingdoms of life. They are small, five-stranded b-barrel protein domains with a common binding face. Over evolutionary time this binding face has been adapted to bind to a wide range of ligands including oligosaccarides, oligonucleotides, proteins and small molecules. In at least one case, enzymatic activity has also evolved at this common face. We have sought to mimic this adaptability in vitro and have developed a range of combinatorial protein libraries based on the OB-fold domain from an aspartyl-tRNA synthetase. We have characterised the protein libraries based on recombinant protein expression and solubility and have then used these libraries in phage display selection for binding back to the native ligand (asp-tRNA) as well as to a prototypic protein ligand (lysozyme). OB-fold variants obtained from selection against lysozyme have been characterised for binding and show moderate affinity (in the micromolar range). The three-dimensional structure has been determined for a single OB-fold variant in complex with lysozyme and this structure provides details of the protein-protein interface along with possible routes to tighter-binding variants. The combination of binding loops and a binding face presented by the OB-fold domain libraries (OBodies), which can be adapted for tailor-made molecular interactions, is a new avenue for exploration in seeking alternatives to antibodies in diagnostics and therapeutics.
Download or read book Molecular Engineering of Selective Recognition Elements as Coatings for Sensor Platforms written by Justyn Wayne Jaworski and published by . This book was released on 2009 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structural and Thermodynamical Basis for Molecular Recognition Between Engineered Binding Proteins written by Jakob Dogan and published by . This book was released on 2006 with total page 57 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analysis of Protein protein Molecular Recognition written by Simon Jeremy Hubbard and published by . This book was released on 1991 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Engineering for Therapeutics written by K. Dane Wittrup and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: These two volumes of Methods in Enzymology cover engineering approaches to the development of protein biopharmaceuticals, which represent a significant and rapidly growing proportion of drug sales. Particular advantages of proteins as drugs relative to small organic molecules include high affinity and specificity afforded by a larger molecular recognition surface and much lower probability of off-target toxicities due to metabolic byproducts. The primary disadvantage to date has been the pharmacokinetic inaccessibility of intracellular drug targets to proteins and peptides, although vigorous efforts at overcoming this limitation are beginning to bear fruit
Download or read book Molecular Recognition and Polymers written by Vincent Rotello and published by John Wiley & Sons. This book was released on 2008-09-15 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: State-of-the-art techniques for tapping the vast potential of polymers The use of specific non-covalent interactions to control polymer structure and properties is a rapidly emerging field with applications in diverse disciplines. Molecular Recognition and Polymers covers the fundamental aspects and applications of molecular recognition—in the creation of novel polymeric materials for use in drug delivery, sensors, tissue engineering, molecular imprinting, and other areas. This reference begins by explaining the fundamentals of supramolecular polymers; it progresses to cover polymer formation and self-assembly with a wide variety of examples, and then includes discussions of biomolecular recognition using polymers. With chapters contributed by the foremost experts in their fields, this resource: Provides an integrated resource for supramolecular chemistry, polymer science, and interfacial science Covers advanced, state-of-the-art techniques used in the design and characterization of non-covalent interactions in polymers Illustrates how to tailor the properties of polymeric materials for various applications Stand-alone chapters address specific applications independently for easy reference. This is a premier resource for graduate students and researchers in polymer chemistry, supramolecular chemistry, materials science, and physical organic chemistry.
Download or read book Protein Engineering written by Jeffrey L. Cleland and published by Wiley-Liss. This book was released on 1996-02-07 with total page 558 pages. Available in PDF, EPUB and Kindle. Book excerpt: This systematic approach to protein engineering provides a thorough introduction to protein biophysics via chapters dealing with the experimental and theoretical determination of protein structure. Separate chapters discuss each of the expression systems c
Download or read book Recognition Receptors in Biosensors written by Mohammed Zourob and published by Springer Science & Business Media. This book was released on 2010-01-08 with total page 864 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recognition receptors play a key role in the successful implementation of chemical and biosensors. Molecular recognition refers to non-covalent speci?c binding between molecules, one of which is typically a macromolecule or a molecular assembly, and the other is the target molecule (ligand or analyte). Biomolecular recognition is typically driven by many weak interactions such as hydrogen bo- ing, metal coordination, hydrophobic forces, van der Waals forces, pi-pi interactions and electrostatic interaction (due to permanent charges, dipoles, and quadrupoles) the polarization of charge distributions by the interaction partner leading to ind- tion and dispersion forces, and Pauli-exclusion-principle-derived inter-atomic repulsion, and a strong, “attractive” force arising largely from the entropy of the solvent and termed the hydrophobic effect. In recent years, there has been much progress in understanding the forces that drive the formation of such complexes, and how these forces are relate to the physical properties of the interacting molecules and their environment allows rational design of molecules and materials that interact in speci?c and desired ways. This book presents a signi?cant and up-to-date review of the various recognition elements, their immobilization, characterization techniques by a panel of dist- guished scientists. This work is a comprehensive approach to the recognition receptors area presenting a thorough knowledge of the subject and an effective integration of these receptors on sensor surfaces in order to appropriately convey the state-of the-art fundamentals and applications of the most innovative approaches.
Download or read book Protein protein Recognition written by Colin Kleanthous and published by Frontiers in Molecular Biology. This book was released on 2000 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of Protein-Protein Recognition is to bring together concepts and systems pertaining to protein-protein interactions in a single unifying volume. In the light of the information from the genome sequencing projects and the increase in structural information it is an opportune time totry to make generalizations about how and why proteins form complexes with each other. The emphasis of the book is on heteromeric complexes (complexes in which each of the components can exist in an unbound state) and will use well-studied model systems to explain the processes of formingcomplexes. After an introductory section on the kinetics, thermodynamics, analysis, and classification of protein-protein interactions, weak, intermediate, and high affinity complexes are dealt with in turn. Weak affinity complexes are represented by electron transfer proteins and integrincomplexes. Anti-lysozyme antibodies, the MHC proteins and their interactions with T-cell receptors, and the protein interactions of eukaryotic signal transduction are the systems used to explain complexes with intermediate affinities. Finally, tight binding complexes are represented by theinteraction of protein inhibitors with serine proteases and by nuclease inhibitor complexes. Throughout the chapters common themes are the technologies which have had the greatest impact, how specificity is determined, how complexes are stabilized, and medical and industrial applications.
Download or read book Molecular Recognition in Proteins written by Iain Kerr and published by . This book was released on 2003 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Engineering of Nanosystems written by Edward A. Rietman and published by Springer Science & Business Media. This book was released on 2001-06-21 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides the professional with an overview of current methodologies in the field, with emphasis on the implementation of current research.
