Download or read book Engineering an Improved Bioconjugation Enzyme written by Anne Zijing Ye and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme engineering is a valuable tool for generating powerful and robust biocatalysts for a broad range of applications. Directed evolution using cell surface display has been previously applied for enzyme engineering, and involves iterative optimization of a protein sequence to yield a desired phenotype. However, previous applications have been carefully tailored for specific enzymes, and this process of developing a good screen is a significant bottleneck in engineering. Co-display of an enzyme and its substrate on the yeast surface is a promising general strategy for evolving bond-forming enzymes, but has only been used to engineer one enzyme. We extended yeast surface co-display to engineer Sfp, a bioconjugation enzyme, for improved activity with a non-native substrate. Sfp library screening identified mutational hotspots in the enzyme and led to the design of Sfp variants with orders of magnitude enhancements in activity. However, these activity improvements were only apparent in contexts similar to yeast surface co-display, reflecting artifacts in the use of this approach for enzyme engineering. Based on our results, we offer suggestions for improving the success of engineering strategies based on yeast surface display.
Download or read book Enzyme Engineering written by Manfred T. Reetz and published by John Wiley & Sons. This book was released on 2023-01-09 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme Engineering An authoritative and up-to-date discussion of enzyme engineering and its applications In Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science, a team of distinguished researchers deliver a robust treatment of enzyme engineering and its applications in various fields such as biotechnology, life science, and synthesis. The book begins with an introduction to different protein engineering techniques, covers topics like gene mutagenesis methods for directed evolution and rational enzyme design. It includes industrial case studies of enzyme engineering with a focus on selectivity and activity. The authors also discuss new and innovative areas in the field, involving machine learning and artificial intelligence. It offers several insightful perspectives on the future of this work. Readers will also find: A thorough introduction to directed evolution and rational design as protein engineering techniques Comprehensive explorations of screening and selection techniques, gene mutagenesis methods in directed evolution, and guidelines for applying gene mutagenesis in organic chemistry, pharmaceutical applications, and biotechnology Practical discussions of protein engineering of enzyme robustness relevant to organic and pharmaceutical chemistry Treatments of artificial enzymes as promiscuous catalysts Various lessons learned from semi-rational and rational directed evolution A transdisciplinary treatise, Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science is perfect for protein engineers, theoreticians, organic, and pharmaceutical chemists as well as transition metal researchers in catalysis and biotechnologists.
Download or read book Biocatalysis Biochemical Fundamentals And Applications Second Edition written by Peter Grunwald and published by World Scientific Publishing Company. This book was released on 2017-08-04 with total page 1314 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this Completely Revised and Extended Edition with a significantly enhanced content, all Chapters have been updated considering relevant literature and recent developments until 2016 together with application oriented examples with a focus on Industrial Biocatalysis. Newly treated topics comprise among others systems metabolic engineering approaches, metagenome screening, new tools for pathway engineering, and de-novo computational design as actual research areas in biocatalysis. Information about different aspects of RNA technologies, and completely new Chapters on 'Fluorescent Proteins' and 'Biocatalysis and Nanotechnology' are also included.
Download or read book Bioconjugate Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 2010-07-26 with total page 1233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions
Download or read book New Enzymes for Organic Synthesis written by Thomas Scheper and published by Springer. This book was released on 2014-01-15 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isolated enzymes are known as useful for synthesising of complex molec ules with more and more large scale applications in recent years. Thes e six reviews show different steps in the development of enzymatic syn theses for useful compounds. Screening of novel enzymes, their biochem ical characterisation as well as reaction engineering approaches in or der to increase their performance are treated in three papers. Then th e use of protein design as a tool for the directed improvement of reac tivity and selectivity of biocatalysts is described in two reviews. Th e supply of sufficient quantities of biocatalysts must not be neglecte d: fluidized bed adsorption as an emerging technology for the simple a nd fast purification of biomolecules is presented in the light of its potential in large scale supply of enzyme catalysts.
Download or read book New Enzymes for Organic Synthesis written by Thomas Scheper and published by Springer Science & Business Media. This book was released on 2007-02-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isolated enzymes are known as useful for synthesising of complex molecules with more and more large scale applications in recent years. These six reviews show different steps in the development of enzymatic syntheses for useful compounds. Screening of novel enzymes, their biochemical characterisation as well as reaction engineering approaches in order to increase their performance are treated in three papers. Then the use of protein design as a tool for the directed improvement of reactivity and selectivity of biocatalysts is described in two reviews. The supply of sufficient quantities of biocatalysts must not be neglected: fluidized bed adsorption as an emerging technology for the simple and fast purification of biomolecules is presented in the light of its potential in large scale supply of enzyme catalysts.
Download or read book Bioconjugation written by Mohammed Aslam and published by . This book was released on 1998 with total page 856 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent years have seen an enormous increase in the use of protein reagents in the biological and medical sciences. Enzyme conjugates and immobilised proteins are fundamental to many diagnostic applications, and existing therapeutic advances are being made using a range of approaches based on antibody conjugates. Protein conjugation techniques are central to the development of these reagents.
Download or read book Emerging Areas in Bioengineering written by Ho Nam Chang and published by John Wiley & Sons. This book was released on 2018-03-12 with total page 898 pages. Available in PDF, EPUB and Kindle. Book excerpt: With more than 40 contributions from expert authors, this is an extensive overview of all important research topics in the field of bioengineering, including metabolic engineering, biotransformations and biomedical applications. Alongside several chapters dealing with biotransformations and biocatalysis, a whole section is devoted to biofuels and the utilization of biomass. Current perspectives on synthetic biology and metabolic engineering approaches are presented, involving such example organisms as Escherichia coli and Corynebacterium glutamicum, while a further section covers topics in biomedical engineering including drug delivery systems and biopharmaceuticals. The book concludes with chapters on computer-aided bioprocess engineering and systems biology. This is a part of the Advanced Biotechnology book series, covering all pertinent aspects of the field with each volume prepared by eminent scientists who are experts on the topic in question. Invaluable reading for biotechnologists and bioengineers, as well as those working in the chemical and pharmaceutical industries. Advanced Biotechnology Biotechnology is a broad, interdisciplinary field of science, combining biological sciences and relevant engineering disciplines, that is becoming increasingly important as it benefits the environment and society as a whole. Recent years have seen substantial advances in all areas of biotechnology, resulting in the emergence of brand new fields. To reflect this progress, Sang-Yup Lee (KAIST, South Korea), Jens Nielsen (Chalmers University, Sweden), and Gregory Stephanopoulos (MIT, USA) have joined forces as the editors of a new Wiley-VCH book series. Advanced Biotechnology will cover all pertinent aspects of the field and each volume will be prepared by eminent scientists who are experts on the topic in question.
Download or read book Enzyme Engineering written by and published by . This book was released on 1998 with total page 696 pages. Available in PDF, EPUB and Kindle. Book excerpt: Records of meetings 1808-1916 in v. 11-27.
Download or read book Membrane Engineering written by Enrico Drioli and published by Walter de Gruyter GmbH & Co KG. This book was released on 2018-12-17 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern membrane science and technology aids engineers in developing and designing more efficient and environmentally-friendly processes. The optimal material and membrane selection as well as applications in the many involved industries are provided. This work is the ideal introduction for engineers working in membrane science and applications (wastewater, desalination, adsorption, and catalysis), process engineers in separation science, biologists and biochemists, environmental scientists, and most of all students. Its multidisciplinary approach also stimulates thinking of hybrid technologies for current and future life-saving applications (artificial organs, drug delivery).
Download or read book Protein Engineering for Improved Enzyme Stability and Selectivity in Asymmetric Transformations Catalyzed by G type Halohydrin Dehalogenases written by Jennifer Solarczek and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzyme Engineering and Evolution Specific Enzyme Applications written by and published by Academic Press. This book was released on 2020-09-15 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Enzymology, Volume 644, the latest release in this ongoing serial, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Site-directed recombination (SDR) in vivo: a fast and reliable tool to unveil beneficial epistasis, Creation and application of amine oxidase with expanded substrate specificities from porcine kidney D-amino acid oxidase, Methods to assess correlation networks for engineering transketolase, Exploration of Enzyme Diversity by Integrating Bioinformatics with Microfluidics, Engineering lytic polysaccharide monooxygenases (LPMOs), Emulsion-based directed evolution of enzymes in yeast, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series
Download or read book Enzyme Engineering XIV written by Allen I. Laskin and published by . This book was released on 1998 with total page 665 pages. Available in PDF, EPUB and Kindle. Book excerpt: Combines fundamental and applied aspects, including preparing novel enzyme systems isolated from nature and using enzymes in a wide variety of industrial processes. A major theme is enzyme technology, which has had a broad impact in biotechnology, synthetic chemistry, pharmaceuticals, and agriculture. Some 120 papers include studies of biosensors for environmental monitoring, fighting nerve-agent chemical weapons with enzyme technology, the kinetics of inactivating enzymes by inert gas bubbling, applying thick-film technology in the mass production of biosensors, developing recombinant bacteria for degrading dibenzothiophene, sorbitol oxidase from microorganisms, improving the stability of soybean and horseradish peroxidases by covalent chemical modification, analyzing glycolysis-relevant compounds in saliva by microbiosensors, and designing and constructing a new nucleozyme. No subject index. Annotation copyrighted by Book News, Inc., Portland, OR
Download or read book Enzyme Engineering written by Manfred T. Reetz and published by John Wiley & Sons. This book was released on 2023-01-10 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme Engineering An authoritative and up-to-date discussion of enzyme engineering and its applications In Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science, a team of distinguished researchers deliver a robust treatment of enzyme engineering and its applications in various fields such as biotechnology, life science, and synthesis. The book begins with an introduction to different protein engineering techniques, covers topics like gene mutagenesis methods for directed evolution and rational enzyme design. It includes industrial case studies of enzyme engineering with a focus on selectivity and activity. The authors also discuss new and innovative areas in the field, involving machine learning and artificial intelligence. It offers several insightful perspectives on the future of this work. Readers will also find: A thorough introduction to directed evolution and rational design as protein engineering techniques Comprehensive explorations of screening and selection techniques, gene mutagenesis methods in directed evolution, and guidelines for applying gene mutagenesis in organic chemistry, pharmaceutical applications, and biotechnology Practical discussions of protein engineering of enzyme robustness relevant to organic and pharmaceutical chemistry Treatments of artificial enzymes as promiscuous catalysts Various lessons learned from semi-rational and rational directed evolution A transdisciplinary treatise, Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science is perfect for protein engineers, theoreticians, organic, and pharmaceutical chemists as well as transition metal researchers in catalysis and biotechnologists.
Download or read book Nanobiomaterial Engineering written by Pranjal Chandra and published by Springer Nature. This book was released on 2020-01-25 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book comprehensively documents the application of Nanobiomaterials in the field of bio-medicine and diagnostics technologies by involving classical concepts/examples. Nanobiotechnology is an emerging area which encompasses all the facets of research of nano and biomaterials with their interaction with biological systems. The book briefly summarizes the various types of Nanomaterial’s, and highlights the recent developments in the synthesis of the nanomaterials for the diagnostic and therapeutic biomedical applications. It skilfully reviews the utilization of the nanomaterials alone or in combination with other bio-molecules as a contrast enhancer in in-vivo imaging, Nano-Theranostics, drug delivery, and sensing transducer matrix. It also discusses the current research on designing of the new Nanobiomaterials and their implementation in numerous fields including bio-medicine and diagnostics. Finally, it summarizes the future prospects and the commercial viability of Nanobiomaterials in the human health care.
Download or read book Enzymes and Coenzymes Advances in Research and Application 2012 Edition written by and published by ScholarlyEditions. This book was released on 2012-12-26 with total page 2604 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes and Coenzymes—Advances in Research and Application: 2012 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Enzymes and Coenzymes. The editors have built Enzymes and Coenzymes—Advances in Research and Application: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Enzymes and Coenzymes in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Enzymes and Coenzymes—Advances in Research and Application: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Download or read book Enzymes in Food Technology written by Mohammed Kuddus and published by Springer. This book was released on 2018-11-19 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: The integration of enzymes in food processing is well known, and dedicated research is continually being pursued to address the global food crisis. This book provides a broad, up-to-date overview of the enzymes used in food technology. It discusses microbial, plant and animal enzymes in the context of their applications in the food sector; process of immobilization; thermal and operational stability; increased product specificity and specific activity; enzyme engineering; implementation of high-throughput techniques; screening of relatively unexplored environments; and development of more efficient enzymes. Offering a comprehensive reference resource on the most progressive field of food technology, this book is of interest to professionals, scientists and academics in the food and biotech industries.
