Download or read book Electrotransformation of Bacteria written by Natalie Eynard and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this manual, protocols for the transformation of about 40 strains of bacteria are described, with the emphasis placed on the individual critical procedural steps, since the practical details mainly depend on the bacterial strain under investigation. This presentation together with the theoretical introductionary chapters, allows users to modify and adapt each protocol to their own experiments. Bacterial strains with relevance in the food industry, biotechnology, medical and veterinary fields, agroindustry and environmental sciences are covered.
Download or read book Handbook of Electroporation written by Damijan Miklavčič and published by Springer. This book was released on 2017-09-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This major reference work is a one-shot knowledge base on electroporation and the use of pulsed electric fields of high intensity and their use in biology, medicine, biotechnology, and food and environmental technologies. The Handbook offers a widespread and well-structured compilation of 156 chapters ranging from the foundations to applications in industry and hospital. It is edited and written by most prominent researchers in the field. With regular updates and growing in its volume it is suitable for academic readers and researchers regardless of their disciplinary expertise, and will also be accessible to students and serious general readers. The Handbook's 276 authors have established scholarly credentials and come from a wide range of disciplines. This is crucially important in a highly interdisciplinary field of electroporation and the use of pulsed electric fields of high intensity and its applications in different fields from medicine, biology, food processing, agriculture, process engineering, energy and environment. An Editorial Board of distinguished scholars from across the world has selected and reviewed the various chapters to ensure the highest quality of this Handbook. The book was edited by an international team of Section Editors: P. Thomas Vernier, Boris Rubinsky, Juergen Kolb, Damijan Miklavcic, Marie-Pierre Rols, Javier Raso, Richard Heller, Gregor Serša, Dietrich Knorr, and Eugene Vorobiev.
Download or read book Streptococcal Genetics written by Joseph J. Ferretti and published by . This book was released on 1987 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electroporation and Electrofusion in Cell Biology written by C.A. Jordan and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells can be funny. Try to grow them with a slightly wrong recipe, and they turn over and die. But hit them with an electric field strong enough to knock over a horse, and they do enough things to justify international meetings, to fill a sizable book, and to lead one to speak of an entirely new technology for cell manipulation. The very improbability of these events not only raises questions about why things happen but also leads to a long list of practical systems in which the application of strong electric fields might enable the merger of cell contents or the introduction of alien but vital material. Inevitably, the basic questions and the practical applications will not keep in step. The questions are intrinsically tough. It is hard enough to analyze the action of the relatively weak fields that rotate or align cells, but it is nearly impossible to predict responses to the cell-shredding bursts of electricity that cause them to fuse or to open up to very large molecular assemblies. Even so, theoretical studies and systematic examination of model systems have produced some creditable results, ideas which should ultimately provide hints of what to try next.
Download or read book Guide to Electroporation and Electrofusion written by Donald C. Chang and published by Academic Press. This book was released on 2012-12-02 with total page 592 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells.Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques. - Covers fundamentals of electroporation and electrofusion in detail: Molecular events, Mechanisms, Kinetics, Gives extensive practical information, The latest applications, Controlling parameters to maximize efficiency, Available instrumentation - Presents applications of electroporation and electrofusion in current research situations - State-of-the-art modifications to electrical pulses and generators - Application of electroporation and electrofusion to unique, alternative cell and tissue types - Gives straightforward, detailed, easy-to-follow protocols for Formation of human hybridomas - Introduction of genetic material into plant cells and pollen - Transfection of mammalian cells - Transformation of bacteria, plants, and yeast - Production of altered embryos - Optimization of electroporation by using reporter genes - Comprehensive and up-to-date - Convenient bench-top format - Approximately 125 illustrations complement the text - Complete references with article titles - Written by leading authorities in electroporation and electrofusion
Download or read book Pseudomonas Methods and Protocols written by Alain Filloux and published by Humana. This book was released on 2016-09-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings.
Download or read book Laboratory Methods in Enzymology DNA written by and published by Elsevier. This book was released on 2013-09-02 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on DNA
Download or read book E coli Plasmid Vectors written by Nicola Casali and published by Humana Press. This book was released on 2010-11-10 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.
Download or read book Bacterial Chemosensing written by Michael D. Manson and published by Humana Press. This book was released on 2018-02-11 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume covers a wide range of up-to-date technologies that have been successfully applied to study the chemosensing behavior of the traditional model species, such as Escherichia coli and Salmonella typhimurium, while being also applicable to a wide spectrum of other species. Beginning with an introduction, the sections of the book explore methods for studying bacterial chemotaxis at the population and whole-cell levels, in vivo analysis of receptor function, cryo-EM methods for studying chemoreceptor structure, as well as intracellular movement of chemosensory proteins, high-throughput methods to screen for novel chemoeffectors, and chemical tools and computer simulations for analyzing chemotaxis. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Chemosensing: Methods and Protocols provides an extensive repertoire of approaches that can be extended to understanding chemotaxis, in particular, and chemosensing, in general, in the context of the enormously varied lifestyles adopted in the larger bacterial world.
Download or read book Laboratory Methods in Enzymology Cell Lipid and Carbohydrate written by and published by Academic Press. This book was released on 2013-10-30 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate
Download or read book Industrial Biotechnology written by Christoph Wittmann and published by John Wiley & Sons. This book was released on 2017-04-10 with total page 789 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest volume in the Advanced Biotechnology series provides an overview of the main production hosts and platform organisms used today as well as promising future cell factories in a two volume book. Alongside describing tools for genetic and metabolic engineering for strain improvement, the authors also impart topical information on computational tools, safety aspects and industrial-scale production. Following an introduction to general concepts, historical developments and future technologies, the text goes on to cover multi-purpose bacterial cell factories, including those organisms that exploit anaerobic biosynthetic power. Further chapters deal with microbes used for the production of high-value natural compounds and those obtained from alternative raw material sources, concluding with eukaryotic workhorses.
Download or read book Irreversible Electroporation in Clinical Practice written by Martijn R. Meijerink and published by Springer. This book was released on 2017-12-27 with total page 281 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive overview of the clinical use of irreversible electroporation (IRE) – better known by its commercial name, NanoKnife – which is one of the most exciting new needle-guided cancer treatments. The coverage includes the history of IRE, general technique, preclinical research, applications in clinical practice and early clinical results, and future perspectives. Contraindications, treatment planning, potential complications, follow-up imaging, and other practical aspects are fully discussed, with highlighting of useful tips and tricks. Through the delivery of short but highly intense electrical pulses, IRE results in tumor cell membrane permeabilization, causing cells to go into apoptosis. The minimally invasive nature of IRE, combined with the prospect of completely eradicating tumors while preserving delicate structures in the ablation zone, makes IRE the object of worldwide clinical research. This book will be of value for practitioners and trainees in interventional and diagnostic radiology, surgery, medical oncology, HPB and gastroenterology, urology, and radiation oncology.
Download or read book Molecular Biological Methods for Bacillus written by Colin R. Harwood and published by Wiley. This book was released on 1991-02-18 with total page 618 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biological Methods for Bacillus Edited by C. R. Harwood, Department of Microbiology, University of Newcastle upon Tyne, UK and S. M. Cutting, The Biological Laboratories, Harvard University, USA This volume represents the first major attempt to produce a compendium of the experimental methods used for the analysis of Bacillus, one of the most important procaryotic genera. Since the pioneering work on the transformation and genetic analysis of Bacillus subtilis by John Spizizen and his colleagues in the late 1950s this microorganism has been extensively studied and is now one of the best understood. More than forty of the world’s leading researchers in the field have generously contributed experimental procedures—devised and used in their laboratories—to this book. The aim throughout has been to present methods as simple step-by-step protocols which have been thoroughly tried and tested. The context in which the methods are used is discussed in detail and relevant information provided on the physiology and genetics of Bacillus. In addition valuable support is provided in the form of troubleshooting tips and advice on safety, the preparation of reagents, and the use of equipment. The book will be invaluable to those working with the genus Bacillus and related genera—both established researchers and those wishing to use this important microorganism for the first time.
Download or read book Fundamental Food Microbiology written by Bibek Ray and published by CRC Press. This book was released on 2007-10-08 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maintaining the high standard set by the previous bestselling editions, Fundamental Food Microbiology, Fourth Edition presents the most up-to-date information in this rapidly growing and highly dynamic field. Revised and expanded to reflect recent advances, this edition broadens coverage of foodborne diseases to include many new and emerging
Download or read book Functional Metagenomics Tools and Applications written by Trevor C. Charles and published by Springer. This book was released on 2017-10-09 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, the latest tools available for functional metagenomics research are described.This research enables scientists to directly access the genomes from diverse microbial genomes at one time and study these “metagenomes”. Using the modern tools of genome sequencing and cloning, researchers have now been able to harness this astounding metagenomic diversity to understand and exploit the diverse functions of microorganisms. Leading scientists from around the world demonstrate how these approaches have been applied in many different settings, including aquatic and terrestrial habitats, microbiomes, and many more environments. This is a highly informative and carefully presented book, providing microbiologists with a summary of the latest functional metagenomics literature on all specific habitats.
Download or read book Plant Microbe Symbiosis written by Ajit Varma and published by Springer Nature. This book was released on 2020-04-01 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an overview of the latest advances concerning symbiotic relationships between plants and microbes, and their applications in plant productivity and agricultural sustainability. Symbiosis is a living phenomenon including dynamic variations in the genome, metabolism and signaling network, and adopting a multidirectional perspective on their interactions is required when studying symbiotic organisms. Although various plant-microbe symbiotic systems are covered in this book, it especially focuses on arbuscular mycorrhiza (AM) symbiosis and root nodule symbiosis, the two most prevalent systems. AM symbiosis involves the most extensive interaction between plants and microbes, in the context of phylogeny and ecology. As more than 90% of all known species of plants have the potential to form mycorrhizal associations, the productivity and species composition, as well as the diversity of natural ecosystems, are frequently dependent upon the presence and activity of mycorrhizas. In turn, root nodule symbiosis includes morphogenesis and is formed by communication between plants and nitrogen-fixing bacteria. The biotechnological application of plant–microbe symbiosis is expected to foster the production of agricultural and horticultural products while maintaining ecologically and economically sustainable production systems. Designed as a hands-on guide, this book offers an essential resource for researchers and students in the areas of agri-biotechnology, soil biology and fungal biology.
Download or read book Desk Encyclopedia of Microbiology written by Moselio Schaechter and published by Academic Press. This book was released on 2010-04-19 with total page 1277 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Desk Encyclopedia of Microbiology, Second Edition is a single-volume comprehensive guide to microbiology for the advanced reader. Derived from the six volume e-only Encyclopedia of Microbiology, Third Edition, it bridges the gap between introductory texts and specialized reviews. Covering topics ranging from the basic science of microbiology to the current "hot" topics in the field, it will be invaluable for obtaining background information on a broad range of microbiological topics, preparing lectures and preparing grant applications and reports. - The most comprehensive single-volume source providing an overview of microbiology to non-specialists - Bridges the gap between introductory texts and specialized reviews - Provides concise and general overviews of important topics within the field making it a helpful resource when preparing for lectures, writing reports, or drafting grant applications
