Download or read book Electrophoretic Studies of Biologically Important Ed Complexes written by Pramod Kumar and published by LAP Lambert Academic Publishing. This book was released on 2012-04 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book entitled with "Determination of Stability Constants of Binary and Mixed Complexes" deals with the stability constants of metal complexes can be very easily calculated by this technique, therefore the present method is advantageous over other methods ( viz, polarographic, potentiometric, solubility etc) which usually suffers from a number of vitiating factors (e.g. diffusion, ionic strength and temperature ). The Electrophoretic technique is almost free from these vitiating factors and results are fairly reliable. Its further extension that these studies can be used to reduced the level of these metal ions in biological system. These studies may also be used for the resolution of racemic mixture of penicillamine and other amino acids.
Download or read book Speciation of Biologically Important Cu II and Ni II Complexes by Capillary and Microchip Electrophoresis written by Karine Faure and published by . This book was released on 2005 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chapter 1 introduces the theory and principles behind capillary electrophoresis separations and the instrumental requirements. An overview of the speciation of metal complexes by capillary electrophoresis is given, detailing the various situations reported in the literature. A summary of the recent advances in the area of microchip electrophoresis is also included, focusing in particular on the challenge of the metal complexes detection in miniaturised systems. Finally, the concept of equilibrium in electrophoretic systems is highlighted, discussing the determination of liabile complexes during electrophoretic processes. Chapter 2 reports one of the first examples of separation by microchip electrophoresis of individual species in equilibrium within a single metal-ligand system. Initial work showed that the effective separation of the three stoichiometries of nickel-bathophenanthroline disulfonic acid (NiBPS) complexes, i.e. [Ni(BPS)1, Ni(BPS)2 and Ni(BPS)3] could be achieved by capillary electrophoresis in less than 5 min. In chapter 3, the research focused on the study of labile copper-histidine equilibrium. A CE method was developed for the separation of copper-histidine complexes and free histidine. Preliminary work on solid phase extraction and molecular imprinted polymer of the metal complex is also presented. Chapter 4 outlines the use of microchip electrophoresis as an efficient tool for the analysis of labile complexes. A 2.5 cm channel was used for the separation of copper-histidine complex and free histidine, where no decomplexation was observed and therefore the exact distribution could be assessed. However, the labile character of copper comples prevented the separation of the two stoichiometries of copper histidine systems. In chapter 5, capillary electrophoresis was explored as a man for the determination of pKa values of drugs, outlining its advantages over traditional techniques. A linear model for the analysis of monoprotic molecules was applied to two acidic (nicotinic acid, naproxen), two basic (adenine and probranolol) and two amphotheric drugs (labetalol, pyridoxine) and compared to values reported previously in literature in order to confirm its validity. This model was then successfully applied to labile drugs, pyrilamine and procaine. Since capillary electrophoresis allows the use of minimal amounts of analytes, the utility of this method was extended to the analysis of synthetically limited non-natural amino acids and novel Sulindac analogues.
Download or read book Clinical Applications of Capillary Electrophoresis written by Terry M. Phillips and published by Humana Press. This book was released on 2012-09-13 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its consumption of minute samples (less than a microlitre), low reagent costs, and extreme sensitivity, depending upon the source of detection used. Clinical Applications of Capillary Electrophoresis aims to give an in-depth manual of CE applications in several important areas of clinical science. Divided into seven sections, this volume provides a brief overview of how CE has been applied in clinical settings, followed by several chapters on CE analysis of important diagnostic molecules and biofluids, as well as descriptions of applications in clinical chemistry, hematology, bacteriology, virology, disease-associated biomarker discovery, immunology and genetic analysis. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Clinical Applications of Capillary Electrophoresis seeks to serve as a valuable source of information not only for clinical pathologists, but also clinical scientists who wish to apply the technique to diagnosis and research.
Download or read book Research Grants Index written by National Institutes of Health (U.S.). Division of Research Grants and published by . This book was released on 1969 with total page 858 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Difference Gel Electrophoresis written by Kay Ohlendieck and published by Springer Nature. This book was released on 2022-11-15 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of this volume provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Download or read book Methods in Membrane Biology written by Edward D. Korn and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although not the only volume in this series in which lipids are discussed, the present volume is devoted entirely to methods for the study of membrane lipids. Even now, when membrane proteins are properly receiving so much attention, this emphasis on membrane lipids is appropriate. Essentially all of the phospholipids and sterols of cells are in membranes. Moreover, although membrane proteins are certainly of utmost importance, the more we learn about the functional properties of membrane proteins, the more we appreciate the unique features of phospholipids, without which biological membranes would be impossible. The hydrophobic-hydrophilic duality of phospholipids allows, indeed requires, their association, in an aqueous environment, into an essentially two-dimensional membrane-only molec ularly thick in one dimension but relatively infinite in the other two; a structure composed of small molecules, not covalently linked, and therefore, infinitely mobile and variable, but yet a structure with great stability and one largely impermeable to most biomolecules. These membrane-forming properties are shared by many amphipathic polar lipids-phospholipids, glycolipids, and sphingolipids-that differ significantly from each other in the nature of their polar head groups and their fatty acids. These variations in structure allow a range of specific interactions among membrane lipids and between lipids and proteins and also provide for membranes of variable, but controlled, fluidity. In this way, phospholipids provide an appropriate milieu for functional membrane proteins and also significantly modulate their catalytic activities.
Download or read book FUNDAMENTALS OF CHEMISTRY Volume I written by Sergio Carrà and published by EOLSS Publications. This book was released on 2009-05-05 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamentals of Chemistry theme in two volumes, is a component of Encyclopedia of Chemical Sciences, Engineering and Technology Resources in the global Encyclopedia of Life Support Systems (EOLSS), which is an integrated compendium of twenty one Encyclopedias. The Theme is organized into six different topics which represent the main scientific areas : History and Fundamentals of Chemistry; Chemical Experimentation and Instrumentation; Theoretical Approach to Chemistry; Chemical Thermodynamics; Rates of Chemical Reactions; Chemical Synthesis of Substances. These two volumes are aimed at the following five major target audiences: University and College students Educators, Professional practitioners, Research personnel and Policy analysts, managers, and decision makers and NGOs
Download or read book High Resolution Separation and Analysis of Biological Macromolecules written by Barry L. Karger and published by Elsevier. This book was released on 1996 with total page 664 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Liquid chromatography * Electrophoresis * Mass spectrometry.
Download or read book Handbook of Capillary Electrophoresis Second Edition written by James P. Landers and published by CRC Press. This book was released on 1996-12-23 with total page 918 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.
Download or read book Agricultural and Biological Chemistry written by and published by . This book was released on 1976 with total page 670 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immuno electrophoresis A New Method for the Analysis of Complex Antigen and Antibody Mixtures Application to Human Serum Antigens and Hyper immune Horse Serum written by Curtis A Williams and published by . This book was released on 1954 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Determination of Complex Reaction Mechanisms written by John Ross and published by Oxford University Press. This book was released on 2006 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt: In a chemical system with many chemical species several questions can be asked: what species react with other species: in what temporal order: and with what results? These questions have been asked for over one hundred years about simple and complex chemical systems, and the answers constitute the macroscopic reaction mechanism. In Determination of Complex Reaction Mechanisms authors John Ross, Igor Schreiber, and Marcel Vlad present several systematic approaches for obtaining information on the causal connectivity of chemical species, on correlations of chemical species, on the reaction pathway, and on the reaction mechanism.Basic pulse theory is demonstrated and tested in an experiment on glycolysis. In a second approach, measurements on time series of concentrations are used to construct correlation functions and a theory is developed which shows that from these functions information may be inferred on the reaction pathway, the reaction mechanism, and the centers of control in that mechanism. A third approach is based on application of genetic algorithm methods to the study of the evolutionary development of a reaction mechanism, to the attainment given goals in a mechanism, and to the determination of a reaction mechanism and rate coefficients by comparison with experiment. Responses of non-linear systems to pulses or other perturbations are analyzed, and mechanisms of oscillatory reactions are presented in detail. The concluding chapters give an introduction to bioinformatics and statistical methods for determining reaction mechanisms.
Download or read book Principles of Molecular Biology written by Burton E. Tropp and published by Jones & Bartlett Publishers. This book was released on 2012-12-14 with total page 781 pages. Available in PDF, EPUB and Kindle. Book excerpt: Includes access to the Student Companion Website with every print copy of the text.Written for the more concise course, Principles of Molecular Biology is modeled after Burton Tropp's successful Molecular Biology: Genes to Proteins and is appropriate for the sophomore level course. The author begins with an introduction to molecular biology, discussing what it is and how it relates to applications in "real life" with examples pulled from medicine and industry. An overview of protein structure and function follows, and from there the text covers the various roles of technology in elucidating the central concepts of molecular biology, from both a historical and contemporary perspective. Tropp then delves into the heart of the book with chapters focused on chromosomes, genetics, replication, DNA damage and repair, recombination, transposition, transcription, and wraps up with translation.Key Features:- Presents molecular biology from a biochemical perspective, utilizing model systems, as they best describe the processes being discussed-Special Topic boxes throughout focus on applications in medicine and technology-Presents "real world" applications of molecular biology that are necessary for students continuing on to medical school or the biotech industry-An end-of-chapter study guide includes questions for review and discussion-Difficult or complicated concepts are called-out in boxes to further explain and simplify
Download or read book Applications of Genetics to Arthropods of Biological Control Significance written by Sudhir Karl Narang and published by CRC Press. This book was released on 2018-01-10 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by experts in the fields of insect pest genetics, the genetics of biological control organisms, and the application of biological control, this book provides the first up-to-date summary of the genetic literature on the genetics of arthropod biological control agents. It identifies successful programs and also gaps and needs in research, research constraints, and possible research approaches in this important field of pest control. The power and applicability of new genetic and molecular biology methods have created new and exciting possibilities to greatly improve the effectiveness of traditional biological control programs. This book provides essential information about the state-of-the-art application of these new methods. It explains how biological control procedures can be improved, covers methods for selecting pesticide-resistant strains of natural enemies, and looks at methods for maintaining genetic diversity and quality control during the rearing of biological control agents in the laboratory. The book also provides information regarding the application of powerful PCR methods for taxonomic identification of strains and species of biocontrol agents.
Download or read book Capillary Electrophoresis of Carbohydrates written by Nicola Volpi and published by Springer Science & Business Media. This book was released on 2010-10-26 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Simple carbohydrates, complex oligosaccharides and polysaccharides all belong to a class of ubiquitous (macro)molecules that exhibit a wide range of biological functions, and the recent advent of enhanced enzymatic, chemical and analytical tools used to study these sugars has inaugurated a genuine explosion in the field of glycomics. Specifically, it has led to a deeper understanding of how specific sugar structures modulate cellular phenotypes, and that breakthrough has led to the discovery of new pharmaceuticals for the treatment of many serious diseases, such as cancer. The subsequent rapid expansion of this research holds high promise for future therapeutic regimens, and capillary electrophoresis (CE) refers to the range of related separation techniques that are integral to this vital research. CE uses narrow-bore fused-silica capillaries to separate a complex array of large and small molecules, and Capillary Electrophoresis of Carbohydrates offers a comprehensive look at the latest breakthroughs and improvements in CE and CE techniques applied to monosaccharides up to complex oligosaccharides and polysaccharides. It begins with an overview of the application of CE and CE- mass spectrometric in the analysis of simple carbohydrates without any previous derivatization step before discussing various detection techniques such as spectrophotometric detection, electrochemical detection and other less common techniques. It then covers in detail an array of related topics and numerous applications. It is an essential text for anyone exploring the myriad possibilities of this rapidly expanding field.
Download or read book Protides of the Biological Fluids written by H. Peeters and published by Elsevier. This book was released on 2014-11-26 with total page 1239 pages. Available in PDF, EPUB and Kindle. Book excerpt: An International Review Series Devoted to Proteins and Related Studies, Volume 32: Protides of the Biological Fluids documents the proceedings of the 32nd Colloquium held in 1984. This compilation is organized into three parts. Part I begins with an introduction to the defense mechanism of immunoglobulins, followed by a description of enzymes and proteins, such as lactoperoxidase and lactoferrin. The secretions that include CSF, colostrum, milk, female and male genital secretions, saliva, gastrointestinal juice, nasal and bronchoalveolar secretions, tears and synovial fluid are also elaborated. The tumor markers, which include ferritin, CEA, and TPA are dealt with in Part II. The immunotoxins, tumor imaging, and lymphoid malignancies are also covered. Part III is devoted to the advances in the separation of protides, including the staining procedure for protein detection, two-dimensional electrophoresis, and HPLC and affinity chromatography. This book is valuable to biology students and clinicians of disciplines related to advances in the protein field.
