Download or read book Electrophoresis of Enzymes written by Gunter M. Rothe and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: The electrophoresis of enzymes and isoenzymes is a well established technique in biochemical, clinical, environmental, microbiological, botanical and forensic laboratories and classical electrophoresis is presently undergoing a remarkable revival. This book compiles facts and methods on enzyme electrophoresis widely dispersed in hundreds of publications. The author summarizes them in clearly readable tables, in many carefully worked out electrophoresis and more than 140 staining protocols. The exhaustive practical experience of the author and the wealth of material summarized and reviewed makes this book a "must" for every enzyme laboratory. It will supply the practitioner with profound information on state-of-the-art enzyme electrophoresis.
Download or read book Handbook of Detection of Enzymes on Electrophoretic Gels written by Gennady P. Manchenko and published by CRC Press. This book was released on 2002-12-26 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first publish
Download or read book Gel Electrophoresis of Proteins written by Michael J Dunn and published by Elsevier. This book was released on 2014-05-12 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gel Electrophoresis of Proteins focuses on the techniques, methodologies, reactions, and approaches involved in gel electrophoresis of proteins. The selection first covers steady-state gel electrophoresis systems and one-dimensional PAA-gel electrophoretic techniques to separate functional and denatured proteins. Discussions focus on affinity electrophoresis, structure and physico-chemical properties of polyacrylamide gels, moving boundary electrophoresis, isotachophoresis, fundamental steady-state electrophoresis systems, and fundamental properties of steady-state electrophoresis systems. The text then reviews conventional isoelectric focusing and immobilized pH gradients and high resolution two-dimensional polyacrylamide-gel electrophoresis. Topics include production of narrow pH gradients, extended pH gradients, polymerization kinetics, IEF in agarose matrices, titration curves, two-dimensional electrophoresis under non-denaturing conditions, and IEF in polyacrylamide matrices. The book tackles quantifying patterns from two-dimensional PAGE, protein staining and detection methods, and immunoelectrophoretic methods. Concerns include post-electrophoretic organic protein stains, silver and enzyme stains, detection of radioactive proteins, and programming languages. The selection is highly recommended for researchers wanting to conduct studies on gel electrophoresis of proteins.
Download or read book Food Microbiology Protocols written by John F. T. Spencer and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Two of the recent books in the Methods in Molecular Biology series, Yeast Protocols and Pichia Protocols, have been narrowly focused on yeasts and, in the latter case, particular species of yeasts. Food Microbiology Pro- cols, of necessity, covers a very wide range of microorganisms. Our book treats four categories of microorganisms affecting foods: (1) Spoilage organisms; (2) pathogens; (3) microorganisms in fermented foods; and (4) microorganisms p- ducing metabolites that affect the flavor or nutritive value of foods. Detailed information is given on each of these categories. There are several chapters devoted to the microorganisms associated with fermented foods: these are of increasing importance in food microbiology, and include one bacteriophage that kills the lactic acid bacteria involved in the manufacture of different foods—cottage cheese, yogurt, sauerkraut, and many others. The other nine chapters give procedures for the maintenance of lactic acid bacteria, the isolation of plasmid and genomic DNA from species of Lac- bacillus, determination of the proteolytic activity of lactic acid bacteria, det- mination of bacteriocins, and other important topics.
Download or read book Principles of Bacterial Pathogenesis written by Eduardo A. Groisman and published by Academic Press. This book was released on 2001-01-09 with total page 863 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles of Bacterial Pathogenesis presents a molecular perspective on a select group of bacterial pathogens by having the leaders of the field present their perspective in a clear and authoritative manner. Each chapter contains a comprehensive review devoted to a single pathogen. Several chapters include work from authors outside the pathogenesis field, providing general perspectives on the evolution, regulation, and secretion of virulence and determinants. Explains the basic principles of bacterial pathogenesis Covers diverse aspects integrating regulation, cellular microbiology and evolution of microbial disease of humans Discusses current strategies for the identification of virulence determinants and the methods used by microbes to deliver virulence factors Presents authoritative treatises of the major disease microorganisms
Download or read book Electrophoresis written by Milan Bier and published by Elsevier. This book was released on 2013-10-22 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrophoresis: Theory, Methods, and Applications, Volume II focuses on the contributions of electrophoresis in the advancement of knowledge on proteins, as well as in the fields of biochemistry, physiology, and medicine. The selection first offers information on the interpretation of electrophoretic mobilities, including theories for other models, electrophoresis of polyelectrolytes, and theory for a rigid spherical particle. The text then takes a look at primary protein structures and nomenclature and identification of the normal human serum proteins. Discussions focus on principles of nomenclature of the serum constituents; methods of identification of an isolated antigen; principal methods used to study serum proteins; separation of mixtures of peptides and amino acids by high-voltage electrophoresis; and methods for determining the primary structure of proteins. The publication elaborates on inheritance of protein variation in human serum and antibodies and myeloma proteins. Topics include products of enzymic digestion, products of reduction, naturally occurring fragments, genetic variation, and variations detected in human serum proteins. The manuscript then examines lymph and cerebrospinal fluid, electrophoresis of gastrointestinal secretions, and high resolution techniques. The selection is a dependable source of data for readers interested in electrophoresis.
Download or read book Gel Electrophoresis and Isoelectric Focusing of Proteins written by Robert C. Allen and published by Walter de Gruyter GmbH & Co KG. This book was released on 2019-05-20 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: No detailed description available for "Gel Electrophoresis and Isoelectric Focusing of Proteins".
Download or read book Electrophoretic Separation of Proteins written by Biji T. Kurien and published by . This book was released on with total page 523 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Gel Electrophoresis of Proteins written by B. D. Hames and published by . This book was released on 1981 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Capillary Electrophoresis of Proteins written by Tim Wehr and published by CRC Press. This book was released on 1998-10-20 with total page 301 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."
Download or read book Practical Protein Electrophoresis for Genetic Research written by George Acquaah and published by . This book was released on 1992 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book enables the novice to understand the "whys" and "hows" of electrophoresis and to initiate and complete an electrophoretic investigation from beginning laboratory organization to publishing results.
Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Download or read book Techniques for Starch Gel Electrophoresis of Enzymes from Forest Tree Species written by W. M. Cheliak and published by [Chalk River, Ont.] : Petawawa National Forestry Institute. This book was released on 1984 with total page 49 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of quantitative characters for crop improvement is essential for phenotypic selection in a breeding program. Relative to polygenic quantitative traits, the electrophoretic study of allozyme variants (alleles) of isozymes associated with specific gene loci allows direct estimates of genetic variation to be made within and among populations. This report describes the process of protein starch gel electrophoresis in the study of forest tree populations. Sections deal with procedures for haploid tissues (megagametophytes from conifers) and diploid tissues. Appendices outline extraction, electrode and gel buffers, and enzyme staining recipes.
Download or read book Electrophoresis in Practice written by Reiner Westermeier and published by John Wiley & Sons. This book was released on 2016-03-16 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fifth edition of the successful, long-selling classic has been completely revised and expanded, omitting some topics on obsolete DNA electrophoresis, but now with a completely new section on electrophoretic micro-methods and on-the-chip electrophoresis. The text is geared towards advanced students and professionals and contains extended background sections, protocols and a trouble-shooting section. It is now also backed by a supplementary website providing all the figures for teaching purposes, as well as a selection of animated figures tested in many workshops to explain the underlying principles of the different electrophoretic methods.
Download or read book Electrophoresis written by Budin Michov and published by Walter de Gruyter GmbH & Co KG. This book was released on 2020-09-07 with total page 1122 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrophoresis is a straightforward but informative analytical method used in biochemistry, biology and medicine. This book combines a detailed discussion of theory and technical application with an elaborate section on troubleshooting and problem solving in electrophoresis. Therefore the book is an important guide for both students and scientists.
Download or read book HPLC in Enzymatic Analysis written by Edward F. Rossomando and published by John Wiley & Sons. This book was released on 2009-09-25 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the first edition of this highly successful book: the role of enzymes in biological research has expanded; the application of HPLC and enzymes has extended to more disciplines; advances in separation techniques and instrumentation have increased the capability of HPLC; and the discovery of new enzymes has spawned new methods of analysis. High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition addresses these developments in its coverage of the refinements of HPLC methods and their use in a wide range of laboratory applications. It offers the same practical approach found in the first edition, incorporates a wealth of new information into existing chapters, and adds new chapters to deal with new applications, including capillary electrophoresis, forensic chemistry, microdialysis, and the polymerase chain reaction. Topics include: * Application of HPLC to the assay of enzymatic activities * Concepts and principles of HPLC, including the latest technological advances * Concepts and principles of capillary electrophoresis (CE) * Strategy for design of an HPLC/CE system for assay of enzyme activity * Preparation of enzymatic activities from tissues and single cells * Analysis of enzymatic activities in body fluids, including chromatobiosis * HPLC for the identification of new enzymatic activities * Fundamentals of the polymerase chain reaction * HPLC in forensics * Survey of enzymatic activities assayed by the HPLC method, including many new categories * Multienzyme systems, including many new examples * HPLC in the analysis of contaminated food "It is the ability of HPLC to accomplish separations completely and rapidly that led to its original application to problems in the life sciences, particularly those related to purification. An analysis of the literature revealed that this technique was used primarily for the purification of small molecules, macromolecules such as peptides and proteins, and more recently, antibodies. This application to purification has all but dominated the use of the method, and there has been a plethora of books, symposia, and conferences on the use of HPLC for these purposes. However, it was only a matter of time before others began to look beyond and to explore the possibilities that result from the capacity to make separations quickly and efficiently." --from the preface to the First Edition Easy to read and full of practical advice and hundreds of diagrams and examples, High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition is an invaluable resource for students, researchers, and laboratory workers in analytical chemistry and biochemistry, molecular biology and cell biology, and for anyone interested in keeping up with this fast-growing field.
Download or read book Neuroproteomics written by Oscar Alzate and published by CRC Press. This book was released on 2009-10-26 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg
