Download or read book Drug membrane Interactions written by Joachim K. Seydel and published by . This book was released on 2002 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Drug Membrane Interactions written by Joachim K. Seydel and published by John Wiley & Sons. This book was released on 2009-07-10 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: Barrier, reservoir, target site - those are but some of the possible functions of biological lipid membranes in the complex interplay of drugs with the organism. A detailed knowledge of lipid membranes and of the various modes of drug-membrane interaction is therefore the prerequisite for a better understanding of drug action. Many of today's pharmaceuticals are amphiphilic or catamphiphilic, enabling them to interact with biological membranes. Crucial membrane properties are surveyed and techniques to elucidate drug-membrane interactions presented, including computer-aided predictions. Effects of membrane interaction on drug action and drug distribution are discussed, and numerous examples are given. This unique reference volume builds on the authors' long experience in the study of drug-membrane interaction. Recommended reading for everyone involved in pharmaceutical research.

Download or read book Drug biomembrane interaction studies written by S. Giatrellis and published by Elsevier Inc. Chapters. This book was released on 2013-10-31 with total page 17 pages. Available in PDF, EPUB and Kindle. Book excerpt: DSC is a non-invasive experimental technique, which, besides other numerous applications, has been extensively applied in investigating the thermodynamic properties of synthetic and biological membranes. The calorimetric profiles of phase transitions of self-organized lipid membranes can provide valuable information about membrane interactions with biomolecules, pharmaceutical agents, other membranes, etc. The scope of this chapter is to review specific applications of DSC in studying membrane– nucleic acid interactions, which have attracted scientific attention for their biological relevance, as well as for their potential for biotechnological and pharmaceutical advances.

Download or read book Development of High throughput Technologies for the Study of Drug membrane Interactions written by and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Drug biomembrane interaction studies written by R. Pignatello and published by Elsevier Inc. Chapters. This book was released on 2013-10-31 with total page 39 pages. Available in PDF, EPUB and Kindle. Book excerpt: This chapter will summarize recent information on cell membranes. Their structure, functions and the role of the various components are discussed, considering both their physiological tasks, such as mechanisms of drug internalization into cells, as well as membrane changes associated with or caused by particular disease states. Later chapters will discuss the possibility of testing biomembrane models to study their interaction with drugs and biological compounds.

Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book In Silico Modelling of Membranes and Drug Membrane Interactions written by Callum Dickson and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Drug biomembrane interaction studies written by R. Pignatello and published by Elsevier Inc. Chapters. This book was released on 2013-10-31 with total page 23 pages. Available in PDF, EPUB and Kindle. Book excerpt: DSC studies of the interaction between drugs or other biologically active compounds with biomembrane models has often been associated or integrated with other analytical methodologies. The information gained from various techniques can depict the different and complex elements that compose such interactions. This chapter will summarize some recent examples of successfully combining DSC with other physico-chemical methods, such as spectroscopy, chromatography, calorimetry, the Langmuir–Blodgett film technique and microscopy.

Download or read book Probing Lipid Membrane Interactions with Drug Molecules and Cationic Proteins Using Combined Experimental and Computational Analysis written by Lorena Alvarez and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The lipid bilayer's integrity is essential for cell function as it acts as the primary barrier against external molecules like drugs and peptides, which can alter the bilayer's physical properties. This dissertation investigates how amphetamine (AMPH) and methamphetamine (METH), and the charged HIV1-TAT peptide impact the stability of lipid bilayers, using a home-built lipid bilayer apparatus that enables real-time monitoring through electrical and fluorescence measurements. Our findings indicate that AMPH and METH increase the lipid bilayer's ion permeability, with METH having a greater destabilizing effect. High concentrations of these stimulants, akin to levels in blood plasma of individuals with stimulant-related brain injuries, lead to pore formation in the bilayer. The extent of destabilization correlated with the drug concentration. We also studied the translocation dynamics of the charged HIV1-TAT peptide across the lipid bilayer. The analysis of current fluctuations showed that successful translocation of the TAT peptide is concentration-dependent, highlighting the significance of charge in inducing membrane deformation or pore formation. Additionally, molecular dynamic simulations were used to explore AMPH interactions with the lipid bilayer in greater detail. The results revealed AMPH's preferred orientation during interaction and its hydrophobic nature, as evidenced by the larger energy barrier encountered in the hydrophilic head group regions of the lipid bilayer. To complement these findings, we utilized surface-enhanced Raman spectroscopy (SERS) to estimate the concentrations of AMPH within lipid bilayers. The data showed a positive correlation between characteristic peak heights and AMPH concentrations. Moreover, whole-cell patch clamp measurements on neuronal cells were employed to examine AMPH's effects in a more intricate lipid environment. This research contributes to the understanding of how stimulants and charged peptides interact with lipid bilayers, which is vital for insights into their biological impacts and in developing therapeutic interventions.

Download or read book In Vitro Drug membrane Interactions Studied by Surface Analytic Methods written by Julius Leo Zieleniecki and published by . This book was released on 19?? with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Drug membrane Interactions written by E. K. Rooney and published by . This book was released on 1986 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biophysical Properties of Catonic phospholipid and Drug membrane Interactions Implications for Targeted Drug Delivery written by Robert B. Campbell and published by . This book was released on 1999 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Electrochemical Studies on Drug Membrane Interaction written by Subramaniam Rameshkumar and published by . This book was released on 2018-04-17 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Role of the phospholipid composition for the drug membrane and protein membrane interactions written by Armelle Varnier Agasøster and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Free Energy Calculations written by Christophe Chipot and published by Springer Science & Business Media. This book was released on 2007-01-08 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Free energy constitutes the most important thermodynamic quantity to understand how chemical species recognize each other, associate or react. Examples of problems in which knowledge of the underlying free energy behaviour is required, include conformational equilibria and molecular association, partitioning between immiscible liquids, receptor-drug interaction, protein-protein and protein-DNA association, and protein stability. This volume sets out to present a coherent and comprehensive account of the concepts that underlie different approaches devised for the determination of free energies. The reader will gain the necessary insight into the theoretical and computational foundations of the subject and will be presented with relevant applications from molecular-level modelling and simulations of chemical and biological systems. Both formally accurate and approximate methods are covered using both classical and quantum mechanical descriptions. A central theme of the book is that the wide variety of free energy calculation techniques available today can be understood as different implementations of a few basic principles. The book is aimed at a broad readership of graduate students and researchers having a background in chemistry, physics, engineering and physical biology.

Download or read book Pharmacology for Anaesthesia and Intensive Care written by Tom E. Peck and published by Cambridge University Press. This book was released on 2008-01-31 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has been thoroughly updated and expanded, with additional contributions from experts in the field, to include all new drugs available to the anaesthetist and intensive care specialist. Basic pharmacological principles are dealt with methodically and with many highly annotated diagrams and tables.

Download or read book Biophysical Interactions of Peptides and Their Analogues with Lipid Membranes written by Anja Stulz and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Many drugs, displaying a wide range of structures and diverse applications, can cross or bind to lipid membranes. Quantitative understanding of membrane interactions is thus important for several therapeutic approaches. First, membrane permeabilization represents the dominating mode of action of antimicrobial peptides (AMPs) and their synthetic mimics (SMAMPs). In terms of clinical applications, selectivity for bacterial over mammalian membranes is as important as good activity. Second, membrane interactions might influence loading, retaining, and releasing drugs from lipid-based drug delivery systems in a time controlled and targeted manner. Understanding the binding behaviour of the peptide drug exenatide to lipid membranes is not only important for characterization of its release from vesicular phospholipid gels, but might also help to understand other complex peptide-lipid interactions. The main aim of this thesis was to derive a mechanistic understanding of interactions of peptides and their analogues with model lipid membranes with a focus on the lipid composition of a membrane. Membrane permeabilization induced by AMPs and SMAMPs was quantified by a lifetime-based leakage assay using calcein-loaded vesicles. Different leakage behaviours were identified by considering active concentrations, strengths of individual leakage events, L1, and cumulative kinetics. Further experiments using isothermal titration calorimetry (ITC), monolayer adsorption measurements, and differential scanning calorimetry (DSC) helped to characterize the initial binding of AMPs and SMAMPs to lipid membranes and their propensity to induce electrostatic lipid clustering. Leakage experiments showed that the leakage behaviour changes with both, the structure of the AMP or SMAMP and the lipid composition of the membrane. The activity seems to increase if a membrane-active agent favours a permeabilization mechanism to which the particular lipid composition is especially susceptible. A closer look at kinetic profiles allowed distinguishing leakage induced by asymmetric stress from leakage events that occur stochastically. Very hydrophobic and unselective compounds seem to act mainly by hydrophobically driven asymmetry stress, especially when acting on zwitterionic phosphatidylcholine (PC) membranes. This mechanism brings about poor selectivity because all lipid membranes (bacterial and mammalian) contain a hydrophobic core. Stochastic leakage events, on the other hand, probably depend more on lipid compositions. Negatively charged lipids like phosphatidylglycerol (PG) or cardiolipin (CL) triggered the initial electrostatic attraction of polycationic AMPs or SMAMPs to bacterial membranes. High amounts of phosphatidylethanolamine (PE) seem to counteract the unselective asymmetry stress mechanism. Finally, especially strong leakage events were induced in vesicles containing CL. In this way, compounds that induce only rare leakage events might still be effective. In the second part of the thesis, an ITC fit model was introduced to study complex peptide-lipid interactions based on primary binding of peptide to the lipid layer and secondary binding to pre-bound peptide. Exenatide served as an exemplary peptide that interacts electrostatically with mixed POPC/POPG liposomes and self-associates at Kd = 46 μM. A global fit of various ITC curves revealed that exenatide binds primarily to a binding site at the outer membrane leaflet composed of 2-3 negatively charged POPG and some POPC molecules. Primary binding showed high affinity with a Kd1 of 0.2-0.6 μM, while secondary binding with a Kd2 of 10-46 μM was weaker. ITC was able to quantify primary and secondary binding separately, based on different binding enthalpies. Unlike ITC, other methods such as tryptophan fluorescence and microscale thermophoresis (MST) probably represent only a summary or average of both effects. Many similar ITC data can be found in literature that possibly involve primary and secondary binding effects. Those data could benefit from a fit model as presented in this thesis