Download or read book DNA Microarrays Part A Array Platforms and Wet Bench Protocols written by and published by Elsevier. This book was released on 2011-08-19 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations. Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician. Provides an overview of platforms Includes experimental design and wet bench protocols Presents statistical and data analysis methods, array databases, data visualization and meta-analysis
Download or read book DNA Microarrays Part B Databases and Statistics written by and published by Academic Press. This book was released on 2006-09-11 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations. Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician. Provides an overview of platforms Includes experimental design and wet bench protocols Presents statistical and data analysis methods, array databases, data visualization and meta analysis
Download or read book DNA Microarrays written by Brian Oliver and published by . This book was released on 2006 with total page 469 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book DNA Microarrays Part B Databases and Statistics written by and published by Elsevier. This book was released on 2006-08-28 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations. Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician. Provides an overview of platforms Includes experimental design and wet bench protocols Presents statistical and data analysis methods, array databases, data visualization and meta analysis
Download or read book DNA Microarrays Part B Databases and Statistics written by and published by Academic Press. This book was released on 2006-09-11 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations. Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician. Provides an overview of platforms Includes experimental design and wet bench protocols Presents statistical and data analysis methods, array databases, data visualization and meta analysis
Download or read book Stem Cell Tools and Other Experimental Protocols written by and published by Elsevier. This book was released on 2006-12-12 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the third of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories. Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering Presents the latest most innovative technologies Addresses therapeutic relevance including FDA compliance and tissue engineering
Download or read book Microarray Technology in Practice written by Steve Russell and published by Academic Press. This book was released on 2008-11-21 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using chips composed of thousands of spots, each with the capability of holding DNA molecules corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome. As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain daunting to many researchers. This guide is designed to demystify the technology and inform more biologists about this critically important experimental technique. Cohesive overview of the technology and available platforms, followed by detailed discussion of experimental design and analysis of microarray experiments Up-to-date description of normalization methods and current methods for sample amplification and labeling Deep focus on oligonucleotide design, printing, labeling and hybridization, data acquisition, normalization, and meta-analysis Additional uses of microarray technology such as ChIP (chromatin immunoprecipitation) with hybridization to DNA arrays, microarray-based comparative genomic hybridization (CGH), and cell and tissue arrays
Download or read book Amyloid Prions and Other Protein Aggregates written by and published by Elsevier. This book was released on 2006-10-06 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability of polypeptides to form alternatively folded, polymeric structures such as amyloids and related aggregates is being increasingly recognized as a major new frontier in protein research. This new volume of Methods in Enzymology along with Part C (volume 413) on Amyloid, Prions and other Protein Aggregates continue in the tradition of the first volume (309) in containing detailed protocols and methodological insights, provided by leaders in the field, into the latest methods for investigating the structures, mechanisms of formation, and biological activities of this important class of protein assemblies. Presents detailed protocols Includes troubleshooting tips Provides coverage on structural biology, computational methods, and biology
Download or read book Advanced Bacterial Genetics Use of Transposons and Phage for Genomic Engineering written by and published by Elsevier. This book was released on 2007-02-27 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard for more than fifty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. This new volume presents methods related to the use of bacterial genetics for genomic engineering. The book includes sections on strain collections and genetic nomenclature; transposons; and phage.
Download or read book Embryonic Stem Cells written by and published by Elsevier. This book was released on 2006-12-08 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories. * Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering * Presents the latest most innovative technologies * Addresses therapeutic relevance including FDA compliance and tissue engineering
Download or read book Adult Stem Cells written by and published by Elsevier. This book was released on 2006-12-11 with total page 575 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the second of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques focusing on adult stem cells, and written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories. Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering Presents the latest most innovative technologies Addresses therapeutic relevance including FDA compliance and tissue engineering
Download or read book Microfluidics Based Microsystems written by S. Kakaç and published by Springer Science & Business Media. This book was released on 2010-09-10 with total page 622 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains an archival record of the NATO Advanced Study Institute on Microfluidics Based Microsystems – Fundamentals and App- cations held in Çe ?me-Izmir, Turkey, August 23–September 4, 2009. ASIs are intended to be high-level teaching activity in scientific and technical areas of current concern. In this volume, the reader may find interesting chapters and various microsystems fundamentals and applications. As the world becomes increasingly concerned with terrorism, early - spot detection of terrorist’s weapons, particularly bio-weapons agents such as bacteria and viruses are extremely important. NATO Public Diplomacy division, Science for Peace and Security section support research, Advanced Study Institutes and workshops related to security. Keeping this policy of NATO in mind, we made such a proposal on Microsystems for security. We are very happy that leading experts agreed to come and lecture in this important NATO ASI. We will see many examples that will show us Microfluidics usefulness for rapid diagnostics following a bioterrorism attack. For the applications in national security and anti-terrorism, microfluidic system technology must meet the challenges. To develop microsystems for security and to provide a comprehensive state-of-the-art assessment of the existing research and applications by treating the subject in considerable depth through lectures from eminent professionals in the field, through discussions and panel sessions are very beneficial for young scientists in the field.
Download or read book DNA Arrays written by Jang B. Rampal and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide instruction in designing and constructing DNA arrays, as well as hybridizing them with biological samples for analysis. An additional purpose is to p- vide the reader with a broad description of DNA-based array technology and its potential applications. This volume also covers the history of DNA arrays—from their conception to their ready off-the-shelf availability—for readers who are new to array technology as well as those who are well versed in this field. Stepwise, detailed experimental procedures are described for constructing DNA arrays, including the choice of solid support, attachment methods, and the general conditions for hybridization. With microarray technology, ordered arrays of oligonucleotides or other DNA sequences are attached or printed to the solid support using au- mated methods for array synthesis. Probe sequences are selected in such a way that they have the appropriate sequence length, site of mutation, and T .
Download or read book Microbial Cell Factories Engineering for Production of Biomolecules written by Vijai Singh and published by Academic Press. This book was released on 2021-02-13 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial Cell Factories Engineering for Production of Biomolecules presents a compilation of chapters written by eminent scientists worldwide. Sections cover major tools and technologies for DNA synthesis, design of biosynthetic pathways, synthetic biology tools, biosensors, cell-free systems, computer-aided design, OMICS tools, CRISPR/Cas systems, and many more. Although it is not easy to find relevant information collated in a single volume, the book covers the production of a wide range of biomolecules from several MCFs, including Escherichia coli, Bacillus subtilis, Pseudomonas putida, Streptomyces, Corynebacterium, Cyanobacteria, Saccharomyces cerevisiae, Pichia pastoris and Yarrowia lipolytica, and algae, among many others. This will be an excellent platform from which scientific knowledge can grow and widen in MCF engineering research for the production of biomolecules. Needless to say, the book is a valuable source of information not only for researchers designing cell factories, but also for students, metabolic engineers, synthetic biologists, genome engineers, industrialists, stakeholders and policymakers interested in harnessing the potential of MCFs in several fields. Offers basic understanding and a clear picture of various MCFs Explains several tools and technologies, including DNA synthesis, synthetic biology tools, genome editing, biosensors, computer-aided design, and OMICS tools, among others Harnesses the potential of engineered MCFs to produce a wide range of biomolecules for industrial, therapeutic, pharmaceutical, nutraceutical and biotechnological applications Highlights the advances, challenges, and future opportunities in designing MCFs
Download or read book Molecular Plant Breeding written by Yunbi Xu and published by CABI. This book was released on 2010 with total page 754 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in plant genomics and molecular biology have revolutionized our understanding of plant genetics, providing new opportunities for more efficient and controllable plant breeding. Successful techniques require a solid understanding of the underlying molecular biology as well as experience in applied plant breeding. Bridging the gap between developments in biotechnology and its applications in plant improvement, Molecular Plant Breeding provides an integrative overview of issues from basic theories to their applications to crop improvement including molecular marker technology, gene mapping, genetic transformation, quantitative genetics, and breeding methodology.
Download or read book Microarrays written by Jang B. Rampal and published by Springer Science & Business Media. This book was released on 2007-07-27 with total page 923 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments. Among the topics discussed in Volume 1: Synthesis Methods, are matrices in the synthesis of microarrays, array optimization processes, array-based comparative genomic hybridization, 60-mer oligonucleotide probes, bifunctional reagents NTMTA and NTPAC, and high density arrays using digital microarray synthesis platforms. Other topics include multiplex ligation-dependent probe amplification (MLPA), hybridization conditions in situ-synthesized oligo arrays, peptide arrays, high density replication tools (HDRT), protocols for the quantification of oligo hybridization, glyco-bead arrays, and an investigation into the emerging nano technology. Microarray Technology, Volumes 1 and 2, provide ample information to all levels of scientists from novice to those intimately familiar with array technology.
Download or read book Microarray Technology Through Applications written by Francesco Falciani and published by Garland Science. This book was released on 2007-06-30 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microarray Technology Through Applications provides the reader with an understanding, from an applications perspective, of the diverse range of concepts required to master the experimental and data analysis aspects of microarray technology. The first chapter is a concise introduction to the technology and provides the theoretical background required to understand the subsequent sections. The following chapters are a series of case studies representative of the most general and important applications of microarray technology, including CGH, analysis of gene expression, SNP arrays and protein arrays. The case studies are written by experts in the field and describe prototypic projects, indicating how to generalize the approach to similar studies. There are detailed step-by-step protocols describing the specific experimental and data analysis protocols mentioned in the case study section. There is also information on printing glass DNA microarray slides and data interpretation. Colour figures and data sets are provided on the website at http://www.garlandscience.com/9780415378536
