Download or read book Dissecting and Disrupting the Interactions Between Host Cells and the Porcine Reproductive and Respiratory Syndrome Virus written by Elle Love McLuskey and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Studies on the Interaction Between Porcine Reproductive and Respiratory Syndrome Virus and Its Natural Host Cell written by Wei Yu Chen and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mechanisms of Porcine Reproductive and Respiratory Syndrome Virus PRRSV Control of the Host Innate Immune Response written by Christopher Overend and published by . This book was released on 2011 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Host virus Interactions in Porcine Reproductive and Respiratory Syndrome Virus Infection written by George Edwin Peter Sorensen and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Host virus Interaction During Porcine Reproductive and Respiratory Syndrome Virus PRRSV Infection written by Hanmo Zhang and published by . This book was released on 2014 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Decoding Protein Networks During Porcine Reproductive and Respiratory Syndrome Virus Infection Through Proteomics written by Laura Sanchez Mendoza and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Porcine reproductive and respiratory virus (PRRSV) is a pathogen of high importance in the porcine industry because it can lead to significant economic losses. One of the cell lines routinely used for research and vaccine production is MARC-145 (African green monkey kidney cells). Molecular interactions between the host cells and the virus are essential to understand how the virus uses the cell machinery to replicate and infect neighbouring cells. Our goal was to analyze the proteomic changes involved during the PRRSV infection in MARC-145 cells, including the composition of the infected cells' virions and exosomes. The infected and non-infected cells' supernatants were purified to obtain the host cells' virions and exosomes. Extracted proteins were further analyzed by High-Resolution-Quadrupole-Hybrid-Orbitrap mass spectrometry and classified according to molecular function and subcellular localization. The need for obtaining reliable proteomics data led to the next goal of optimizing the infection of MARC-145 cells with PRRSV. To assess the efficiency of the infection, we synchronized the infection, used a virus tagged with an enhanced green fluorescent protein (EGFP) and added polycations at different concentrations to stimulate the binding of the viral particles to the cell. To verify the percentage of infected cells, we used fluorescence microscopy and flow cytometry. Our findings suggest the cellular proteins affected during the PRRSV infection could play important roles in host immune response and/or the viral life cycle. The efficiency of the use of polycations was demonstrated to be effective in increasing PRRSV infection.

Download or read book Characterization of Interactions Between Porcine Reproductive and Respiratory Syndrome Virus and Its Intracellular Environment written by Julie Ann Hicks and published by . This book was released on 2012 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Interactions of the Porcine Reproductive and Respiratory Syndrome Virus with Cellular Proteins written by Cheng Song and published by . This book was released on 2010 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Dissection of Virus host Cell Interactions in the Early Response to Infection written by and published by . This book was released on 2014 with total page 51 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book CEO Compensation Information Asymmetry and Other Characteristics written by and published by . This book was released on 2015 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Interactions of Porcine Circovirus Type 2 and Porcine Reproductive and Respiratory Syndrome Virus written by Avanti Sinha and published by . This book was released on 2011 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Novel Pathogenic Mechanisms of Porcine Reproductive and Respiratory Syndrome Virus written by Rui Guo and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Porcine reproductive and respiratory syndrome virus (PRRSV) causes a tremendous economic loss in swine industry worldwide. The capabilities to evade host immune responses and to establish persistent infection are the two hallmark features of PRRSV infection. In this dissertation, the research was mainly focused on investigating the novel mechanisms underlying PRRSV transmission and persistence. In chapter 2, the research was focused on an alternative pathway of PRRSV intercellular transmission. Our data showed that intercellular nanotube connections can be utilized for cell-to-cell spreading the core infectious viral machinery (viral RNA, certain replicases and structural proteins) of PRRSV. Live-cell movies tracked the intercellular transport of a recombinant PRRSV that expressed green fluorescent protein (GFP)-tagged nsp2 in a receptor-independent manner. The cytoskeleton proteins F-actin and myosin-IIA were identified as co-precipitates with PRRSV nanotube associated proteins. Drugs inhibiting actin polymerization or myosin-IIA activation prevented nanotube formations and viral clusters in virus-infected cells. These data lead us to propose that PRRSV utilizes the host cell cytoskeletal machinery inside nanotubes for efficient cell-to-cell spread. This form of virus transport represents an alternative pathway for virus spread, which is resistant to the host humoral immune response. In chapter 3, we further showed that PRRSV infection could induce the formation of nanotubes between infected and uninfected cells following a ROS-dependent nanotube formation model. Co-culturing PRRSV-infected cells with uninfected cells rescued PRRSV-induced cell death. Mitochondrion was observed transferring from uninfected to PRRSV-infected cells. Importantly, impaired formation of nanotube or defective mitochondrion was unable to rescue infected cells from apoptosis/necrosis. Certain PRRSV proteins were detected to associate with mitochondria and transport from infected to uninfected cells through TNTs. Our results suggest that TNTs-transfer of functional mitochondria rescued PRRSV-infected cells from apoptosis/necrosis in the early stage of infection. On the other hand, mitochondria could be utilized as a cargo to transport viral materials for spreading the infection. In chapter 4, a novel mechanism s of PRRSV persistent infection has been studied. In this study, a cellular model of persistent infection was established. Strand-specific quantitative RT-PCR and RNase I treatment analysis showed that double-stranded RNA (dsRNA) conformation existed in persistently infected cells. This data has been further confirmed in vivo by performing two independent PRRSV persistence studies. Immunohistochemistry analysis showed that viral dsRNAs were detected aggregating inside the germinal centers of tonsils and lymph nodes from PRRSV persistence pigs, but RNA array analysis further showed that dsRNA in lymphoid tissues had limited ability to stimulate host antiviral responses during persistent infection stage. These results suggest that the PRRSV dsRNA functions as a mediator for viral persistence. The viral dsRNA persistence in germinal centers of lymphoid tissues may reveal a novel mechanism for PRRSV to escape antiviral immune responses. In summary, this study investigated two novel pathogenic mechanisms of PRRSV infection, which could provide insights on the development of effective control strategies.

Download or read book Investigation of the Interaction of European Porcine Reproductive and Respiratory Syndrome Virus with Porcine Antigen presenting Cells written by Luke P. M. Johnson and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Bases for Modulation of Host Cellular Responses by Porcine Reproductive and Respiratory Syndrome Virus Non structural Protein 11 written by and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Host cellular responses to viruses written by Jue Liu and published by Frontiers Media SA. This book was released on 2023-02-10 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Polymicrobial Diseases written by Kim A. Brogden and published by . This book was released on 2002 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Polymicrobial diseases, those involving more than one etiologic agent, are more common than is generally realized and include respiratory diseases, gastroenteritis, conjunctivitis, keratitis, hepatitis, periodontal diseases, multiple sclerosis, genital infections, intra -- abdominal infections, and pertussis.