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Book Automated Aberration Correction for STED Microscopy

Download or read book Automated Aberration Correction for STED Microscopy written by Debadrita Ghosh and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stimulated emission depletion (STED) microscopy is a powerful super resolution fluorescence microscopy technique that surpasses the diffraction limit. However, the performance of a STED microscope can be adversely influenced by aberrations. In thick biological specimens, aberrations are primarily induced by wavefront distortion caused by variations in the refractive index within the specimen. These aberrations have a substantial impact on the quality of the acquired images and impose limitations on the achievable resolution. This problem can be resolved by using adaptive optics (AO) in a fe...

Book Adaptive Optics Wide field Microscopy Using Direct Wavefront Sensing

Download or read book Adaptive Optics Wide field Microscopy Using Direct Wavefront Sensing written by Oscar A. Azucena and published by . This book was released on 2011 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Adaptive Optics for Biological Imaging

Download or read book Adaptive Optics for Biological Imaging written by Joel A Kubby and published by CRC Press. This book was released on 2013-04-26 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Adaptive Optics for Biological Imaging brings together groundbreaking research on the use of adaptive optics for biological imaging. The book builds on prior work in astronomy and vision science. Featuring contributions by leaders in this emerging field, it takes an interdisciplinary approach that makes the subject accessible to nonspecialists who want to use adaptive optics techniques in their own work in biology and bioengineering. Organized into three parts, the book covers principles, methods, and applications of adaptive optics for biological imaging, providing the reader with the following benefits: Gives a general overview of applied optics, including definitions and vocabulary, to lay a foundation for clearer communication across disciplines Explains what kinds of optical aberrations arise in imaging through various biological tissues, and what technology can be used to correct for these aberrations Explores research done with a variety of biological samples and imaging instruments, including wide-field, confocal, and two-photon microscopes Discusses both indirect wavefront sensing, which uses an iterative approach, and direct wavefront sensing, which uses a parallel approach Since the sample is an integral part of the optical system in biological imaging, the field will benefit from participation by biologists and biomedical researchers with expertise in applied optics. This book helps lower the barriers to entry for these researchers. It also guides readers in selecting the approach that works best for their own applications.

Book Optical Microscopic and Spectroscopic Techniques Targeting Biological Applications

Download or read book Optical Microscopic and Spectroscopic Techniques Targeting Biological Applications written by Vicente Micó and published by Frontiers Media SA. This book was released on 2021-10-22 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Adaptive Optics for Diffraction limited Infrared Imaging with 8 M Telescopes

Download or read book Adaptive Optics for Diffraction limited Infrared Imaging with 8 M Telescopes written by D-G. Sandler and published by . This book was released on 1992 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Aberration Correction in STED Microscopy

Download or read book Aberration Correction in STED Microscopy written by Joris van Dort and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last decade, superresolution microscopy techniques became a valuable tool to study sub-cellular structures with a resolution below the diffraction limit. The use of superresolution for brain research requires to super-resolve structures deep within the tissue of the living mouse brain. The only superresolution technique applied in the living mouse brain so far is stimulated emission depletion (STED) microscopy. However, the penetration depth of STED microscopy in the cortex of a living mouse has been limited to a depth of 40æm. In this thesis, I studied the use of adaptive optics wit ...

Book High Resolution Imaging in Microscopy and Ophthalmology

Download or read book High Resolution Imaging in Microscopy and Ophthalmology written by Josef F. Bille and published by Springer. This book was released on 2019-08-13 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.

Book Measurement and Correction of Aberrations in Light and Electron Microscopy

Download or read book Measurement and Correction of Aberrations in Light and Electron Microscopy written by Jonas Rolf Hans Binding and published by . This book was released on 2012 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: Imperfections in image formation, called aberrations, often preclude microscopes from reaching diffraction-limited resolution. Aberrations can be caused either by the microscope itself or by the sample and can be compensated for by using an active element integrated into the beam path which is functioning as a corrector. The optimal settings for this corrector need to be determined without excessive damage to the sample. In particular, for sensitive biological samples, the potential gain for signal and/or resolution needs to be weighed against sample damage. Here I present the development of a special type of optical coherence microscopy (called deep-OCM), which allows the precise determination of the average rat brain refractive index in vivo. The conclusion is that two-photon microscopy is affected by optical aberrations in this sample starting at depths around 200 μm. Deep-OCM is well suited for imaging myelinated nerve fibers. Individual fibers can be visualized in the living brain in unprecedented depths beyond 300 μm. In the second part of this thesis I describe the development and testing of an auto-focuser and auto-stigmator (called MAPFoSt) for a scanning electron microscope to ensure optimal imaging quality after switching samples or during long acquisition series. MAPFoSt determines the three focus and stigmation parameters from only two test images.

Book Breaking the Diffraction Limit

Download or read book Breaking the Diffraction Limit written by Korbinian J. Kaltenecker and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Blind Deconvolution for Confocal Laser Scanning Microscopy

Download or read book Blind Deconvolution for Confocal Laser Scanning Microscopy written by Praveen Pankajakshan and published by . This book was released on 2009 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: Confocal laser scanning microscopy is a powerful technique for studying biological specimens in three dimensions (3D) by optical sectioning. Although ubiquitous, there are uncertainties in the observation process. As the system's impulse response or point-spread function (PSF) is dependent on both the specimen and imaging conditions, it should be estimated from the observed images along with the object. This problem is ill-posed, under-determined, and as the measurement process is quasi-random in nature, we treat the problem by using Bayesian inference. The state of the art déconvolution and blind déconvolution algorithms are reviewed within a Bayesian framework. In the first part, we recognize that the diffraction-limited nature of the objective lens and the intrinsic noise are the primary distortions that affect this specimen images. An alternative minimization (AM) approach restores the lost frequencies beyond the diffraction limit by using a total variation regularization on the objet, and a spatial constraint on the PSF. Additionally, some methods are proposed to ensure positivity of estimated intensities, conserve the object's flux, and to handle the regularization parameter. When imaging thick specimens, the phase of the pupil function due to spherical aberration (SA) cannot be ignored; It is shown to be dependent on the refractive index mismatch between the object and the objective immersion medium, and the depth under the cover slip. The imaging parameters and the object's original intensity distribution is recovered by modifying the AM algorithm. Due to the incoherent nature of fluorescence microscopy, phase retrieval from the observed intensities is possible by constraining the phase using geometrical optics. This method could be extended to restore specimens affected by SA. As the PSF is space varying, a quasi-convolution model is proposed, and the PSF approximated so that, apart from the object, there is only one free parameter to be estimated.

Book Cell Biological Applications of Confocal Microscopy

Download or read book Cell Biological Applications of Confocal Microscopy written by and published by Academic Press. This book was released on 2003-01-03 with total page 521 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures

Book Optical Coherence Tomography and Its Non medical Applications

Download or read book Optical Coherence Tomography and Its Non medical Applications written by Michael Wang and published by BoD – Books on Demand. This book was released on 2020-05-27 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical coherence tomography (OCT) is a promising non-invasive non-contact 3D imaging technique that can be used to evaluate and inspect material surfaces, multilayer polymer films, fiber coils, and coatings. OCT can be used for the examination of cultural heritage objects and 3D imaging of microstructures. With subsurface 3D fingerprint imaging capability, OCT could be a valuable tool for enhancing security in biometric applications. OCT can also be used for the evaluation of fastener flushness for improving aerodynamic performance of high-speed aircraft. More and more OCT non-medical applications are emerging. In this book, we present some recent advancements in OCT technology and non-medical applications.