Download or read book Developmental Biology Protocols written by Rocky S. Tuan and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences. Though modern developmental biology has its roots in “experimental embry- ogy” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.
Download or read book Developmental Biology Protocols written by Rocky S. Tuan and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in “experimental emb- ology” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.
Download or read book Developmental Biology Protocols written by Rocky S. Tuan and published by Humana Press. This book was released on 2000-01-21 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences. Though modern developmental biology has its roots in “experimental embry- ogy” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.
Download or read book Current Protocols in Molecular Biology written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Developmental Biology Protocols Volumes I II and III written by Rocky S. Tuan and published by Humana Press. This book was released on 1999-12-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fast-growing field of developmental biology provides a unifying framework that integrates anatomy, physiology, genetics, biochemistry, cellular and molecular biology, as well as evolutionary biology. The number of "reference" publications that deal specifically with the practical aspects of experimental developmental biology are relatively scarce. Developmental Biology Protocols grows out of this need for a comprehensive laboratory manual that provides the readers with the principles, background, rationale, as well as practical protocols, for studying and analyzing the events of embryonic development. Its highly practical format and wide range of model systems and multidisciplinary experimental techniques attest to the authors' determination to provide a balanced presentation of both background information and actual laboratory details. This three-volume compilation of relevant and useful information will be a well-utilized resource for both the students and teachers of developmental biology at all levels. Developmental Biology Protocols offers the most comprehensive, cutting-edge collection of contemporary experimental methods available for the study of embryogenesis and development. Written by an interdisciplinary team of leading scientific investigators, this authoritative collection provides step-by-step instructions for successful laboratory execution. Topics range from animal model system acquisition to molecular genetics, and include high resolution imaging, transgenesis, teratology, comparative anatomy and embryology, the characterization of embryonic structure and function, the analysis of cell function, and the regulation of gene activity. In addition to many unique, new experimental protocols, the authors have also utilized a host of experimental approaches used in other disciplines applicable to the study of development. Comprehensive and richly annotated, Developmental Biology Protocols constitutes the gold-standard reference for today's developmental biologists.
Download or read book Developmental Biology Protocols written by Rocky S. Tuan and published by Springer Science & Business Media. This book was released on 2000 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: Annotation This book combines expert reviews of the best contemporary methods for studying the disease process in ovarian cancer with detailed technical protocols for its analysis. The book focuses on the major problems still facing researchers on reproducible experimental methods to illuminate those problems and on gene therapeutic approaches to its treatment.
Download or read book Molecular Methods in Developmental Biology written by Matt Guille and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: The process whereby a single cell, the fertilized egg, develops into an adult has fascinated for centuries. Great progress in understanding that process, h- ever, has been made in the last two decades, when the techniques of molecular biology have become available to developmental biologists. By applying these techniques, the exact nature of many of the interactions responsible for forming the body pattern are now being revealed in detail. Such studies are a large, and it seems ever-expanding, part of most life-science groups. It is at newcomers to this field that this book is primarily aimed. A number of different plants and animals serve as common model org- isms for developmental studies. In Molecular Methods in Developmental Bi- ogy: Xenopus and Zebrafish, a range of the molecular methods applicable to two of these organisms are described, these are the South African clawed frog, Xenopus laevis, and the zebrafish, Brachydanio rerio. The embryos of both of these species develop rapidly and externally, making them particularly suited to investigations of early vertebrate development. However, both Xenopus and zebrafish have their own advantages and disadvantages. Xenopus have large, robust embryos that can be manipulated surgically with ease, but their pseudotetraploidy and long generation time make them unsuitable candidates for genetics. This disadvantage may soon be overcome by using the diploid Xenopus tropicalis, and early experiments are already underway. The transp- ent embryos of zebrafish render them well-suited for in situ hybridization and immunohistochemistry, and good for observing mutations in genetic screens.
Download or read book Plant Developmental Biology written by Lars Hennig and published by Humana. This book was released on 2016-08-23 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants come in myriads of shapes and colors, and the beauty of plants has fascinated mankind for thousands of years. Long before Mendel discovered the laws of heritab- ity and Darwin developed his theory on evolution, the affection for ornamental plants led people to select alleles that establish novel plant forms. Today, plant developmental biology tries to discover the mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant’s life. Although the underlying processes of cell proliferation and differentiation are similar in plants and a- mals, plants are different because their development is usually open, and its outcome is not the faithful repetition of a general plan but is strongly in?uenced by environm- tal conditions. In the last few decades, plant developmental biology has pinpointed a large number of developmental regulators and their interactions and the mechanisms that govern plant development start to emerge. In part, this progress was enabled by the advance of powerful molecular tools for a few model species, most importantly Arabidopsis. This volume of the Methods in Molecular Biology series provides a collection of protocols for many of the common experimental approaches in plant developmental bi- ogy. All chapters are written in the same format as that used in the Methods in Molecular TM Biology series. Each chapter opens with a description of the basic theory behind the method being described.
Download or read book Molecular Embryology written by Paul T. Sharpe and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 752 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most people have some interest in embryos; this probably results, in part, from their interest in understanding the biological origins of themselves and their offspring and, increasingly, concerns about how environmental change such as pollution might affect human development. Obviously, et- cal considerations preclude experimental studies of human embryos and, c- sequently, the developmental biologist has turned to other species to examine this process. Fortunately, the most significant conclusion to be drawn from the experimental embryology of the last two decades is the manner in which orthologous or closely related molecules are deployed to mediate similar - velopmental processes in both vertebrates and invertebrates. The molecular mechanisms regulating processes fundamental to most animals, such as axial patterning or axon guidance, are frequently conserved during evolution. (It is now widely believed that the differences between phyla and classes are the result of new genes, arising mostly by duplication and divergence of extant sequences, regulating the appearance of derived characters. ) Other vertebrates are obviously most likely to use the same devel- mental mechanisms as humans and, within the vertebrate subphylum, the - parent degree of conservation of developmental mechanism is considerable. It has long been recognized that particular vertebrate species offer either d- tinct advantages in investigating particular stages of development or are - pecially amenable to particular manipulations. No single animal can provide all the answers because not all types of experiments can be carried out on a single species.
Download or read book Cell Biology Protocols written by J. Robin Harris and published by Wiley. This book was released on 2012-01-18 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a modern composite scientific discipline, Cell Biology has expanded and moved forward rapidly in recent years. Cell Biologists now require a wide range of techniques, including those of analytical biochemistry and microscopy in all its diverse forms. These are often used alongside the techniques of molecular biology and molecular genetics. This book contains numerous useful protocols, covering light and electron microscopy, cell culture, cell separation, subcellular fractionation, organelle and membrane isolation, and the use of in vitro reassembly systems in Cell Biology. Many of these protocols feature helpful notes and safety information for practical application. The format favours easy use at the bench with space for notes and important safety information. An appendix contains essential analytical information that will prove invaluable to those working on all aspects of cell biology. This book will be of interest to students and more experienced cell biologists, as well as molecular biologists and those working in genomics and proteomics who are looking for cellular techniques to validate their findings within intact cells.
Download or read book Methods in Developmental Biology written by Fred H. Wilt and published by . This book was released on 1967 with total page 872 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cellular and Molecular Procedures in Developmental Biology written by and published by Academic Press. This book was released on 1997-11-17 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures. Provides straightforward, current protocols and critical appraisals Includes diverse analysis of cellular and molecular techniques Presents everything from whole-organ cultures to electrophysiological approaches Details a variety of methods for interfering with gene function in various species Offers multiple illusions of in situ hybridization, immunostaining, and apoptosis
Download or read book Chemical Biology written by Jonathan E. Hempel and published by Humana. This book was released on 2016-09-24 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential components of the chemical biology tool discovery process, the book is organizes into four parts that focus on platforms for molecular discovery in in vitro cellular systems, in vivo chemical genetic screening protocols, and methods used to discover functional protein targets. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and informative, Chemical Biology: Methods and Protocols seeks to improve the success rate of the chemical biology field through the dissemination of detailed and experiential knowledge.
Download or read book Brain Development written by Simon G. Sprecher and published by . This book was released on 2019 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain.
Download or read book Craniofacial Development written by Sebastian Dworkin and published by . This book was released on 2022 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and in vivo cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using in-situ hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Craniofacial Development: Methods and Protocols aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques. .
Download or read book Essential Zebrafish Methods Cell and Developmental Biology written by Monte Westerfield and published by Academic Press. This book was released on 2009-08-27 with total page 581 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world’s leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of the cellular and developmental biology of zebrafish. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and “tricks of the trade to both experienced researchers and more junior members in the lab. Provides busy researchers a quick reference for time-tested methods and protocols that really work, updated where possible by the original authors Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error
Download or read book Experimental Developmental Biology written by Laura R. Keller and published by Academic Press. This book was released on 1999 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work is designed for use as a lab manual in college-level courses in developmental biology or animal development. In each exercise, students examine gametes and developing embryos of a single species, and also perform several experiments to probe its developmental process.
