Download or read book Development of Biosensors Based on DNA Aptamers for Direct Mycotoxins Detection written by Nawel Mejri and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This aim of this work is to develop ultrasensitive electrochemical biosensors with high affinity toward ochratoxine (OTA) and aflatoxine M1 (AFM1). In order to obtain the best analytical performances, we associated nano-materials in the transducer construction: conducting polypyrrole polymer and poly(amido-amine) dendrimères. Thanks to this association, we benefited from the conducting material's electrical properties, and the large active detection surface dendrimers. For the bimolecular sensing part, we used specific DNA aptamers which allowed us to quantify mycotoxines at nM concentrations. In addition, the different aptamer based biosensors present a very large dynamic ranges. We also demonstrated through the use of different sizes of dendrimers, that the sensitivity depend not only in the affinity between bioreceptors and their target molecules, but also in the physico-chemical properties of the biosensor.

Download or read book Biosensors Based on Aptamers and Enzymes written by Man Bock Gu and published by Springer. This book was released on 2014-07-08 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

Download or read book Nanomycotoxicology written by Mahendra Rai and published by Academic Press. This book was released on 2019-08-24 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanomycotoxicology: Treating Mycotoxins in Nanoway discusses the role of nanotechnology in the detection, toxicity and management of different types of mycotoxins. Sections cover the topic of nanomycotoxicology, the application of nanotechnology for quicker, more cost-effective and precise diagnostic procedures of mycotoxins and toxicogenic fungi, and the application of nanotechnology for the management of mycotoxigenic fungi. New topics, such as the application of nanotechnology in disease management, disease forecasting, and disease resistance, mycotoxin detection, and nanodiagnostic and molecular techniques are also presented. With chapter contributions from an international group of experts, this book presents an interdisciplinary reference for scientists and researchers working in mycotoxicology, nanotechnology, mycology, plant science, and food safety. In addition, it will be a useful tool for industrial scientists investigating technologies to update their nanotoxicology and nanosafety knowledge. Discusses the role of nanotechnology in mycotoxicology Explores the application of nanomaterials for detection of mycotoxins Covers the role of nanotechnology in the management of mycotoxins and mycotoxigenic fungi

Download or read book Development of Nucleic Acid Aptamer Based Sensors for the Direct Detection and Identification of Biological Agents written by and published by . This book was released on 2005 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this work is to develop a novel sensing platform for the direct and rapid detection of biological agents. To achieve this goal, we are building on our portable DNA Biological Detection System work by incorporating a new class of molecular recognition elements, known as aptamers. In this paper, the nucleic acid-based sensor work conducted in our laboratory is discussed including initial work on the design and optimization of aptamer biorecognition sensor elements targeting a relevant biological agent surrogate (i.e., Bacillus globigii) and the development of a portable nucleic acid detection system.

Download or read book Biosensors for Security and Bioterrorism Applications written by Dimitrios P. Nikolelis and published by Springer. This book was released on 2016-03-12 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive coverage of biomarker/biosensor interactions for the rapid detection of weapons of bioterrorism, as well as current research trends and future developments and applications. It will be useful to researchers in this field who are interested in new developments in the early detection of such. The authors have collected very valuable and, in some aspects indispensable experience in the area i.e. in the development and application of portable biosensors for the detection of potential hazards. Most efforts are centered on the development of immunochemical assays including flow-lateral systems and engineered antibodies and their fragments. In addition, new approaches to the detection of enzyme inhibitors, direct enzymatic and microbial detection of metabolites and nutrients are elaborated. Some realized prototypes and concept devices applicable for the further use as a basis for the cooperation programs are also discussed.There is a particular focus on electrochemical and optical detection systems,including those employing carbon nanotubes, quantum dots and metalnanoparticles. The authors are well-known scientists and most of them are editors of respected international scientific journals. Although recently developed biosensors utilize known principles, the biosensing devices described can significantly shorten the time required for successful detection and enhance efforts in more time-consuming directions, e.g. remote sensing systems and validation in real-sample analysis.The authors describe advances in all stages of biosensor development: theselection of biochemical components, their use in biosensor assembly, detection principles and improvements and applications for real sample assays.

Download or read book Aptamer based Biosensors for Protein Biomarkers written by Alan Ka Ho Cheng and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The technique of "systematic evolution of ligands by exponential enrichment" has transformed the premise of biorecognition to encompass nucleic acids in addition to antibodies.These synthetic counterparts - aptamers - are DNA or RNA strands that have been selected through iterative rounds of in vitro selection to bind with affinity to analytes (from small molecules to proteins) of choice. In this thesis, the voltammetric aptamer-based detection of lysozyme was first explored based on charge density changes on aptamer-modified electrodes upon analyte binding. Different optical and electrochemical methods were then tested for the detection of ubiquitous glycanhydrolaselysozyme was first explored based on charge density changes on aptamer-modified electrodes upon analyte binding. Different detection methodologies (optical and electrochemical methods) were then tested for the detection of MUC1(mucin 1),a glycoprotein overexpressed in elevated concentrations in the serum of patients with epithelial adenocarcinomas; this augments the importance for MUC1 detection as an alternative for ealry cancer diagnostics. Optical biosensors developed for the MUC1 detection was through a DNA hybridization scheme with quantum-dot based flouorescence readout. Electrochemical methods were based on either direct attachment of a redox center, methylene blue(MB), to the aptamer strands,or adding free MB to the electrolyte solution(intercalative binding). In both cases, quantitative detection of MUC1 was possible, although direct labeling showed a better sensitivity. To further improve the sensitivity, an intricate biochemical assay was explored featuring the fusion of anti-thrombin and anti-MUC1 aptamers, in the hope of generating a pico-Molar assay for MUC1 via the MUC1- dependent allosteric upregulation of the proteolytic activity of human a-thrombin.

Download or read book Aptamers written by Gulab Singh Yadav and published by Springer Nature. This book was released on 2019-11-11 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book discusses the basics of aptamers and the advent of aptamer-based technology in recent times. The book covers the diverse applications of aptamers, such as in detection of animal and plant pathogens, disease diagnosis and therapeutics, environmental contamination detection etc. Besides these applications, the book also describes the use of these synthetic or modified DNA, as drug delivery vehicles. The different chapters describe how the binding capacity and specificity of aptamers can be exploited in various ways. The book also discusses how these attributes of aptamers can outdo the antibody technology in biomedical and diagnostic solutions. This crisp and concise book gives the readers an insight into the most recent biotechnological applications of aptamers. ​

Download or read book Advanced Biosensors for Health Care Applications written by Dr. Inamuddin and published by Elsevier. This book was released on 2019-06-15 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Biosensors for Health Care Applications highlights the different types of prognostic and diagnostic biomarkers associated with cancer, diabetes, Alzheimer's disease, brain and retinal diseases, cardiovascular diseases, bacterial infections, as well as various types of electrochemical biosensor techniques used for early detection of the potential biomarkers of these diseases. Many advanced nanomaterials have attracted intense interests with their unique optical and electrical properties, high stability, and good biocompatibility. Based on these properties, advanced nanoparticles have been used as biomolecular carriers, signal producers, and signal amplifiers in biosensor design. Recent studies reported that there are several diagnostic methods available, but the major issue is the sensitivity and selectivity of these approaches. This book outlines the need of novel strategies for developing new systems to retrieve health information of patients in real time. It explores the potential of nano-multidisciplinary science in the design and development of smart sensing technology using micro-nanoelectrodes, novel sensing materials, integration with MEMS, miniaturized transduction systems, novel sensing strategy, that is, FET, CMOS, System-on-a-Chip (SoC), Diagnostic-on-a-Chip (DoC), and Lab-on-a-Chip (LOC), for diagnostics and personalized health-care monitoring. It is a useful handbook for specialists in biotechnology and biochemical engineering. Describes advanced nanomaterials for biosensor applications Relates the properties of available nanomaterials to specific biomarkers applications Includes diagnosis and electrochemical studies based on biosensors Explores the potential of nano-multidisciplinary science to design and develop smart sensing technologies Describes novel strategies for developing a new class of assay systems to retrieve the desired health information

Download or read book Aflatoxins written by Irineo Torres-Pacheco and published by BoD – Books on Demand. This book was released on 2011-10-21 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is divided into three sections. The section called Aflatoxin Contamination discusses the importance that this subject has for a country like the case of China and mentions examples that illustrate the ubiquity of aflatoxins in various commodities The section Measurement and Analysis, describes the concept of measurement and analysis of aflatoxins from a historical prespective, the legal, and the state of the art in methodologies and techniques. Finally the section entitled Approaches for Prevention and Control of Aflatoxins on Crops and on Different Foods, describes actions to prevent and mitigate the genotoxic effect of one of the most conspicuous aflatoxins, AFB1. In turn, it points out interventions to reduce identified aflatoxin-induced illness at agricultural, dietary and strategies that can control aflatoxin. Besides the preventive management, several approaches have been employed, including physical, chemical biological treatments and solvent extraction to detoxify AF in contaminated feeds and feedstuffs.

Download or read book Environmental Biosensors written by Vernon Somerset and published by BoD – Books on Demand. This book was released on 2011-07-18 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a collection of contributions from leading specialists on the topic of biosensors for health, environment and biosecurity. It is divided into three sections with headings of current trends and developments; materials design and developments; and detection and monitoring. In the section on current trends and developments, topics such as biosensor applications for environmental and water monitoring, agro-industry applications, and trends in the detection of nerve agents and pesticides are discussed. The section on materials design and developments deals with topics on new materials for biosensor construction, polymer-based microsystems, silicon and silicon-related surfaces for biosensor applications, including hybrid film biosensor systems. Finally, in the detection and monitoring section, the specific topics covered deal with enzyme-based biosensors for phenol detection, ultra-sensitive fluorescence sensors, the determination of biochemical oxygen demand, and sensors for pharmaceutical and environmental analysis.

Download or read book Biosensors for Virus Detection written by Adil Denizli and published by IOP Publishing Limited. This book was released on 2021-12-13 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developments and applications of biosensor platforms for analysis of viral infections including Coronavirus, HIV, Hepatitis, Ebola, Zika, Norovirus, Influenza, SARS etc. Embraces properties, fabrication, and recent research regarding optical, electrochemical, piezoelectric, fluorescence, thermal, magnetic and micromechanical sensor families.

Download or read book Myconanotechnology Green Chemistry for Sustainable Development written by Savita and published by Bentham Science Publishers. This book was released on 2022-07-21 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: Myconanotechnology is the interface between mycology andnanotechnology. In other words, myconanotechnology represents the greensynthesis of nanoparticles using fungi. The field is recently gaining attentiondue to the simple, resource efficient, and ecofriendly nature of fungal biotechnology.Therefore, Myconanotechnology is at the core of cost-effective and sustainablesolutions for many industrial processes. This volume provides readers at all academic levels with a broadbackground on some of the fastest developing areas in myconanotechnology. It isorganised into two sections, A and B. Section A updates readers on severalcutting-edge aspects of the synthesis and characterization of nanoparticlesthrough the use of fungi. Section B describes applications of myconanotechnologyincluding: the management of bacterial and fungal diseases, pest control, amongother applications in medicine and agriculture. The breadth of topics covered inthe contents make this volume an informative resource on the field. Contributionsare written by experts in industrial biotechnology, and include extensivereferences to published studies. This book is a timely reference for researchers, teachers and students,and all readers who are interested in new developments in industrial mycologyand nanotechnology.

Download or read book Aptamers for Analytical Applications written by Yiyang Dong and published by John Wiley & Sons. This book was released on 2019-02-11 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential guide that puts the focus on method developments and applications in aptamers In recent years, aptamer-based systems have been developed for a wide-range of analytical and medical applications. Aptamers for Analytical Applications offers an introduction to the topic, outlines the common protocols for aptamer synthesis, as well as providing information on the different optimization strategies that can obtain higher affinities to target molecules. The contributors?noted experts on the topic?provide an in-depth review of the characterization of aptamer-target molecule interaction and immobilization strategies and discuss the developments of methods for all the relevant applications. The book outlines different schemes to efficiently immobilize aptamers on substrates as well as summarizing the characterization methods for aptamer-ligand complexes. In addition, aptamer-based colorimetric, enzyme-linked, fluorescent, electrochemical, lateral flow and non-labeling analytical methods are presented. The book also reflects state-of-the-art and emerging applications of aptamer-based methods. This important resource: -Provides a guide to aptamers which provide highly specific and sensitive molecular recognition, with affinities in the range of antibodies and are much cheaper to produce -Offers a discussion of the analytical method developments and improvements with established systems and beyond -Offers a comprehensive guide to all the relevant application areas -Presents an authoritative book from contributors who are noted experts in the field Written for analytical chemists, biochemists, analytical researchers, Aptamers for Analytical Applications is a comprehensive book that adopts a methodological point of view to the important aspects of aptamer generation and modification with a strong emphasis on method developments for relevant applications.

Download or read book Nanotechnology Applications for Tissue Engineering written by Sabu Thomas and published by William Andrew. This book was released on 2015-01-03 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue engineering involves seeding of cells on bio-mimicked scaffolds providing adhesive surfaces. Researchers though face a range of problems in generating tissue which can be circumvented by employing nanotechnology. It provides substrates for cell adhesion and proliferation and agents for cell growth and can be used to create nanostructures and nanoparticles to aid the engineering of different types of tissue. Written by renowned scientists from academia and industry, this book covers the recent developments, trends and innovations in the application of nanotechnologies in tissue engineering and regenerative medicine. It provides information on methodologies for designing and using biomaterials to regenerate tissue, on novel nano-textured surface features of materials (nano-structured polymers and metals e.g.) as well as on theranostics, immunology and nano-toxicology aspects. In the book also explained are fabrication techniques for production of scaffolds to a series of tissue-specific applications of scaffolds in tissue engineering for specific biomaterials and several types of tissue (such as skin bone, cartilage, vascular, cardiac, bladder and brain tissue). Furthermore, developments in nano drug delivery, gene therapy and cancer nanotechonology are described. The book helps readers to gain a working knowledge about the nanotechnology aspects of tissue engineering and will be of great use to those involved in building specific tissue substitutes in reaching their objective in a more efficient way. It is aimed for R&D and academic scientists, lab engineers, lecturers and PhD students engaged in the fields of tissue engineering or more generally regenerative medicine, nanomedicine, medical devices, nanofabrication, biofabrication, nano- and biomaterials and biomedical engineering. Provides state-of-the-art knowledge on how nanotechnology can help tackling known problems in tissue engineering Covers materials design, fabrication techniques for tissue-specific applications as well as immunology and toxicology aspects Helps scientists and lab engineers building tissue substitutes in a more efficient way

Download or read book Mycotoxins and Food Safety written by Suna Sabuncuoglu and published by BoD – Books on Demand. This book was released on 2020-10-07 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt: Foodborne illnesses are a global public health concern with implications worldwide. Mycotoxins are naturally occurring toxins produced by microfungi that are capable of causing disease and death in. living organisms. They are recognized as a major economic problem due to their impact on human health, animal productivity, and.domestic and.international.trade. This book provides updated information about foodborne mycotoxins, their toxicities, new determination methods, prevention strategies, and regulations around the world.

Download or read book Electrochemical Biosensors written by Serge Cosnier and published by CRC Press. This book was released on 2015-01-26 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since four decades, rapid detection and monitoring in clinical and food diagnostics and in environmental and biodefense have paved the way for the elaboration of electrochemical biosensors. Thanks to their adaptability, ease of use in relatively complex samples, and their portability, electrochemical biosensors now are one of the mainstays of analy

Download or read book Past Present and Future Challenges of Biosensors and Bioanalytical Tools in Analytical Chemistry A Tribute to Professor Marco Mascini written by and published by Elsevier. This book was released on 2017-08-19 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: Past, Present and Future Challenges of Biosensors and Bioanalytical Tools in Analytical Chemistry: A Tribute to Professor Marco Mascini, Volume 77, the newest release in the Comprehensive Analytical Chemistry series, provides an update to topics of interest, with this volume containing chapters on Biosensors and Bioanalytical tools, Enzymes: from bioreceptors in biosensing to power generation for biosensing, Whole-cell biosensors and bioassays, New trends in antibody-based biosensors, Aptamer-based biosensors and bioassays, Biomimetic sensors based on molecularly imprinted interfaces, Nucleic acid in genosensors and genoassays, Nanomaterials-based platforms for environmental monitoring, and Mobile analytics: Smart-phone based biosensors. Contains contributions from the best authors in the field Provides an essential resource for analytical chemists