Download or read book Development of a Real time PCR Assay for the Detection of Campylobacter Jejuni and Campylobacter Coli written by and published by . This book was released on 2009 with total page 105 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Development and Optimization of a RT PCR Assay for Rapid Campylobacter Spp Detection and Quantification in Poultry Rinsates written by Aaron Bodie and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Campylobacter is a foodborne pathogen mainly associated with poultry products. Currently, prevalence-based data is used to determine process control during poultry processing. However, this method is not sensitive enough, labor intensive and does not provide information on the level and subsequent risk of Campylobacter contamination. As a result, reliable alternative methods are imperative to detect and quantify Campylobacter contamination throughout the harvest process. Therefore, the goal of this dissertation was to develop and optimize a real-time polymerase chain reaction (RT-PCR) assay for rapid detection and quantification of Campylobacter spp. in poultry rinsates using the United States Department of Agriculture - Food Safety Inspection Services protocol and sampling techniques. The first project was focused on identifying the compositional diversity of pooled colonies enumerated from post-chill poultry carcass samples inoculated with Campylobacter jejuni, coli, and lari on various selective and non-selective media, Campy-Cefex, mCCDA, and TSA; (Chapter 2). The objective of the second study was to generate growth curves for Campylobacter jejuni, coli, and lari under the same growth conditions recommended by the International standard organization (Chapter 3). The last chapter aimed to develop and verify a rapid quantification method for Campylobacter species (CampyQuant[TM]) in post-chill poultry rinsates using the BAX® System Real-Time PCR Assay for Campylobacter (Chapter 4). The results of this dissertation were that BAX® System CampyQuant[TM] assay was able to detect Campylobacter jejuni, coli, and lari after 20 h enrichment and quantify the pre-enrichment concentration in a range from 1.00 to 4.00 Log10 CFU/mL. The study suggests that the CampyQuant[TM] BAX® RT-PCR assay can provide the food industry with a rapid, accurate, and efficient alternative method for Campylobacter spp. enumeration to ensure that process controls are working adequately to provide safe products to consumers.

Download or read book Specific Detection of Campylobacter Jejuni and Campylobacter Coli by Using Polymerase Chain Reaction written by and published by . This book was released on 1992 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt: Development of a routine detection assay for Campylobacter jejuni and Campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction(PCR). An oligonucleotide primer pair from a conserved 5' region of the flaA gene of C. coli VC167 was used to amplify a 450-bp region by PCR. The primer pair specifically detected 4 strains of C.coli and 47 strains C. jejuni; but it did not detect strains of Campylobacter fetus, Campylobacter lari, Campylobacter upsaliensis, Campylobacter cryaerophila, Campylobacter butzleri, Campylobacter hyointestinalis, Wolinella recta, Helicobacter pylori, Escherichia coli, Shigella spp., Salmonella spp., Bibrio cholerae, Citrobacter freundii, or Aeromonas spp. By using a non radioactively labeled probe internal to the PCR product, the assay could detect as little as 0.0062 pg of purified C. coli in DNA, or the equivalent of four bacteria. In stools steeded with C. coli cells, the probe could detect between 30 and 60 bacteria per PCR assay. the assay was also successfully used to detect C. coli in rectal swab specimens form experimentally infected rabbits and C.jejuni in human stool samples.

Download or read book The Development of Real time Polymerase Chain Reaction for the Detection of Campylobacter Jejuni written by and published by . This book was released on 2008 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Real time PCR Assay for the Rapid Detection and Quantification of Campylobacter Jejuni in Broilers written by Aradom Debretsion and published by . This book was released on 2006 with total page 55 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Rapid and Reliable Detection of Foodborne Pathogens written by and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Campylobacter Jejuni written by Irving Nachamkin and published by Amer Society for Microbiology. This book was released on 1992 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book the first major text on Camplyobacter infections in over 8 years, summarizes the major advances in understanding the clinical disease and epidemiology of infection. Probably the most common cause of sporadic bacterial diarrheal illness, this pathogen accounts for an estimated 2.4 million cases annually in the United States.

Download or read book Molecular Detection of Foodborne Pathogens written by Dongyou Liu and published by CRC Press. This book was released on 2009-07-28 with total page 905 pages. Available in PDF, EPUB and Kindle. Book excerpt: While the vast majority of our food supplies are nutritious and safe, foodborne pathogen-related illness still affects millions of people each year. Large outbreaks of foodborne diseases- such as the recent salmonella outbreak linked to various peanut butter products- continue to be reported with alarming frequency.All-Encompassing Guide to Detecti

Download or read book Developments in Campylobacter Helicobacter Related Organisms Research CHRO 2019 written by Ozan Gundogdu and published by Frontiers Media SA. This book was released on 2021-02-11 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Antimicrobial Resistance in Bacteria of Animal Origin written by Frank M. Aarestrup and published by Amer Society for Microbiology. This book was released on 2006-01 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antimicrobial Resistance in Bacteria of Animal Origin comprehensively examines the current research on antimicrobial resistance in the main veterinary and zoonotic pathogens, including resistance to disinfectants and metals used in agriculture.

Download or read book Helicobacter Pylori in the 21st Century written by Philip Sutton and published by CABI. This book was released on 2010 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a broad overview of the current understanding of Helicobacter pylori emphasizing its world health impacts. It includes 13 chapters organized into 3 parts: "Pathologies and Treatment", "Host Response" and "Bacterial Defence". Part I (5 chapters) reviews the epidemiology of H. pylori infection, H. pylori infection in Asia, gastric adenocarcinoma, antimicrobial resistance and approaches to treatment, and extragastric manifestations of H. pylori infection. Part II (4 chapters) describes H. pylori-induced acquired immunity and immunoregulation, host genetic factors and susceptibility to H. pylori pathogenesis, innate immune initiators and effectors in H. pylori infection, and H. pylori vaccines. Part III (4 chapters) discusses the importance of H. pylori lipopolysaccharides in gastric adaptation and pathogenesis, H. pylori virulence factors, H. pylori adhesion to the gastric surface, and helicobacteromics. This book is an essential resource for researchers, students and medics in infectious and Helicobacter-associated diseases.

Download or read book Microbiology Laboratory Guidebook written by United States. Food Safety and Inspection Service. Microbiology Division and published by . This book was released on 1998 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Campylobacter in Poultry Physiology Genetics and Detection Methods written by Steven C. Ricke and published by Frontiers Media SA. This book was released on 2022-11-29 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Food Borne Pathogens written by Catherine Adley and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in BiotechnologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Download or read book Advances in Campylobacter Research and Treatment 2011 Edition written by and published by ScholarlyEditions. This book was released on 2012-01-09 with total page 86 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Campylobacter Research and Treatment: 2011 Edition is a ScholarlyBrief™ that delivers timely, authoritative, comprehensive, and specialized information about Campylobacter in a concise format. The editors have built Advances in Campylobacter Research and Treatment: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Campylobacter in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Advances in Campylobacter Research and Treatment: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Download or read book Microbial Source Tracking written by Jorge W. Santo Domingo and published by Emerging Issues in Food Safety. This book was released on 2007 with total page 285 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a state-of-the-art review of the current technology and applications being utilized to identify sources of fecal contamination in waterways. - Serves as a useful reference for researchers in the food industry, especially scientists investigating etiological agents responsible for food contamination. - Provides background information on MST methods and the assumptions and limitations associated with their use. - Covers a broad range of topics related to MST, including environmental monitoring, public health and national security, population biology, and microbial ecology. - Offers valuable insights into future research directions and technology developments.

Download or read book Practical Handbook of Microbiology written by Lorrence H Green and published by CRC Press. This book was released on 2021-05-04 with total page 2032 pages. Available in PDF, EPUB and Kindle. Book excerpt: Practical Handbook of Microbiology, 4th edition provides basic, clear and concise knowledge and practical information about working with microorganisms. Useful to anyone interested in microbes, the book is intended to especially benefit four groups: trained microbiologists working within one specific area of microbiology; people with training in other disciplines, and use microorganisms as a tool or "chemical reagent"; business people evaluating investments in microbiology focused companies; and an emerging group, people in occupations and trades that might have limited training in microbiology, but who require specific practical information. Key Features Provides a comprehensive compendium of basic information on microorganisms—from classical microbiology to genomics. Includes coverage of disease-causing bacteria, bacterial viruses (phage), and the use of phage for treating diseases, and added coverage of extremophiles. Features comprehensive coverage of antimicrobial agents, including chapters on anti-fungals and anti-virals. Covers the Microbiome, gene editing with CRISPR, Parasites, Fungi, and Animal Viruses. Adds numerous chapters especially intended for professionals such as healthcare and industrial professionals, environmental scientists and ecologists, teachers, and businesspeople. Includes comprehensive survey table of Clinical, Commercial, and Research-Model bacteria. The Open Access version of this book, available at http://www.taylorfrancis.com, has been made available under a Creative Commons Attribution-Non Commercial-No Derivatives 4.0 license. Chapter 21, "Archaea," of this book is freely available as a downloadable Open Access PDF under a Creative Commons Attribution-Non Commercial-No Derivatives 4.0 license available at http://www.taylorfrancis.com See Emanuel Goldman's Open Access article: "Lamarck redux and other false arguments against SARS-CoV-2 vaccination," https://www.embopress.org/doi/full/10.15252/embr.202254675