Download or read book Detection of Campylobacter Fetus in Bovine Preputial Scrapings Using PCR and Culture Assays written by Tracy Schmidt and published by . This book was released on 2008 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: The traditional method for the diagnosis of bovine genital campylobacteriosis is the culture and identification of the causative organism, Campylobacter fetus subsp. venerealis (Cfv) from the genital tract. This approach is considered relatively insensitive due to the fragility of the bacteria, their specific nutritional and atmospheric requirements and their being easily overgrown by commensal bacteria. The identification of isolates is also problematic due to the limited biochemical activity of the bacteria. With the rapid advances made in the molecular field, assays have become more robust and cost-effective making them feasible for the diagnostic laboratory. The potential speed, sensitivity and specificity offered by these assays provide attractive alternatives for the identification of pathogens which are notoriously difficult to identify. The first part of this investigation was concerned with the implementation and evaluation of a polymerase chain reaction (PCR) assay for the direct detection of C. fetus in bovine preputial specimens. The specificity of a published C. fetus-specific primer pair was established by testing C. fetus reference and field isolates in addition to a collection of other Campylobacter species and organisms which may encountered in the genital tract of cattle. All C. fetus isolates tested yielded a single PCR amplicon of approximately 750 bp. No amplicons were generated when any of the other non-C. fetus isolates were tested. Following minor modifications to the assay, the sensitivity of the assay was determined using spiked Weybridge medium. A detection limit of 615 Cfv/mR Weybridge medium (or 6,15 cell equivalents per PCR assay) was obtained. Preputial material collected and submitted for laboratory testing may often be contaminated with faeces, urine, semen and/or blood. All of these components are known to be potential PCR inhibitors and the influence of each, on the sensitivity of the PCR assay, was subsequently evaluated. Faeces were identified as a potent inhibitor and contamination of specimens with as little as 1% (w/v) faeces reduced the sensitivity of the assay. Concentrations of up to 50% (v/v) of blood, urine and semen had no effect on the sensitivity of the assay. Preputial specimens, collected in Weybridge medium, were subsequently pooled and spiked and used to establish the sensitivity of both the PCR and culture methods as well as determine the influence of time on the sensitivity of the assays. Testing was carried out in triplicate on samples collected from different herds which were ascertained to be free of Cfv based on the use of specific selection criteria. The detection limit of the culture method was found to be better than that achieved using PCR only immediately after the samples were spiked. The detection limit of the culture method decreased with time whilst the detection limit of the PCR assay remain unchanged up to 72 hours post-inoculation. Ensuing field evaluation involved the testing of 212 clinical samples using both the culture method and the optimized PCR assay. Of the samples tested 4,2% were found to be positive using the PCR assay, whilst only 3,8% were found to be positive by culture. Based upon this evaluation the analytical specificity of the PCR assay was calculated to be 99% and the analytical sensitivity, 85,7%. The second part of this investigation was concerned with the subspeciation of C. fetus isolates. Currently the only test recommended by the Office International des Epizooties (OIE) for the subspeciation of isolates, is tolerance to 1% glycine. Doubts over the reliability of this test have led to alternative or supplementary tests being sought. Within the context of this investigation a collection of 40 South African field isolates were subspeciated using a previously described subspecies-specific primer set as well as the traditional 1% glycine tolerance phenotyping test. Additionally, other phenotyping tests (selenite reduction, growth at 42 °C and susceptibility to metronidazole and cefoperazone) were evaluated to determine their suitability for use as an aid in the subspeciation of C. fetus isolates. None of the field isolates yielded a Cfv-specific subspecies PCR amplicon using the published primer set suggesting that all of the isolates were Campylobacter fetus subsp. fetus (Cff). Based on tolerance to 1% glycine however, only 6 isolates were identified as Cff (glycine tolerant), whilst the remainder were classified as Cfv. The results of the "sensitive" hydrogen sulphide test indicated that the Cfv isolates were specifically Cfv biovar intermedius. The lack of agreement between the PCR and the phenotyping subspeciation results concur with the findings reported by other researchers. It is consequently concluded that the published VenSF/VenSR subspecies-primer set is unsuitable for the subspeciation of South African field isolates.
Download or read book American Journal of Veterinary Research written by and published by . This book was released on 2014 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volumes for 1956- include selected papers from the proceedings of the American Veterinary Medical Association.
Download or read book Bovine Reproduction written by Richard M. Hopper and published by John Wiley & Sons. This book was released on 2021-07-21 with total page 1236 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bovine Reproduction A complete resource for practical, authoritative information on all aspects of bovine theriogenology The newly revised Second Edition of Bovine Reproduction delivers a comprehensive overview of all major issues in bovine reproduction. Written by leading experts in the subject, the book is an indispensable reference for any veterinarian dealing with bovine fertility. Bovine Reproduction is divided into sections on the bull, the cow, the neonate, and assisted reproduction techniques. New chapters cover new gene manipulation technologies, managing problem donors, lameness, and more. Outdated and redundant information from the First Edition has been removed and replaced by coverage of new diseases, technologies, procedures, techniques, and approaches to fertility problems. A new companion website provides images and tables from the book in PowerPoint format. In addition to more than 675 full-color images, readers will also benefit from: A thorough discussion of the anatomy and physiology of the bull, including the endocrine and exocrine function of bovine testes and the thermoregulation of the testes An exploration of breeding and health management of bulls, including the evaluation of breeding soundness and ultrasound examination of the reproductive tract An examination of the anatomy, physiology, and the breeding and health management of cows, including fetal programming, the reproductive tract microbiome, and a section on obstetrics and reproductive surgery A review of the management of both critical care of the neonate and effective colostrum assessment and provision An introduction to assisted and advanced reproductive technologies A practical and comprehensive reference, Bovine Reproduction is a must-have purchase for bovine practitioners, theriogenologists, animal scientists, veterinary students, and residents with an interest in cattle.
Download or read book Immunopathological Studies of Bulls Vaccinated And or Challenged with Tritrichomonas Foetus written by Eduardo Ruben Cobo and published by . This book was released on 2008 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Arthur s Veterinary Reproduction and Obstetrics E Book written by David E. Noakes and published by Elsevier Health Sciences. This book was released on 2009-04-23 with total page 960 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Veterinary Reproduction and Obstetrics has been the standard reference textbook for veterinary students for many years, as well as for students of animal science and related disciplines; in addition it has also been a useful reference source for the practicing veterinary surgeon. The new edition builds on the success of the previous edition covering normal reproduction and reproductive disorders and diseases in the common and less common domesticated species (llamas, alpacas, camels). The book has been completely revised with full colour throughout to include recent developments in reproductive biology and endocrinology, as well as the new knowledge on the causes and treatment of reproductive disease." "This is a reference text that has been refreshed well and warrants a place on practice bookshelves." - Veterinary Record, Feb 2011 Classic text reference Covering all aspects of reproduction and obstetrics in all common and less common domestic species Only book covering full range of domestic animals Practical clinical approach throughout Thorough updating throughout to reflect changes in practice since the last edition New authors and contributors to ensure contemporary and international approach (contributors from Finland, the Netherlands, USA, Denmark an New Zealand) Full colour throughout
Download or read book Blackwell s Five Minute Veterinary Consult Ruminant written by Christopher Chase and published by John Wiley & Sons. This book was released on 2017-10-23 with total page 975 pages. Available in PDF, EPUB and Kindle. Book excerpt: Blackwell's Five-Minute Veterinary Consult: Ruminant, Second Edition keeps practitioners completely current with the latest in disease management for ruminants and camelids. Updates the first all-in-one ruminant resource designed specifically for quick information retrieval Provides identically formatted topics for easy searching by alphabetical listing or by discipline, with each topic indicating the species affected Offers fast access to the accumulated wisdom of hundreds of veterinary experts Adds more than 100 new topics, with significant revisions to existing topics Includes access to a companion website with additional topics, client education handouts, and figures
Download or read book Veterinary Microbiology and Microbial Disease written by P. J. Quinn and published by John Wiley & Sons. This book was released on 2011-10-17 with total page 932 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbiology is one of the core subjects for veterinary students, and since its first publication in 2002, Veterinary Microbiology and Microbial Disease has become an essential text for students of veterinary medicine. Fully revised and expanded, this new edition updates the subject for pre-clinical and clinical veterinary students in a comprehensive manner. Individual sections deal with bacteriology, mycology and virology. Written by an academic team with many years of teaching experience, the book provides concise descriptions of groups of microorganisms and the diseases which they cause. Microbial pathogens are discussed in separate chapters which provide information on the more important features of each microorganism and its role in the pathogenesis of diseases of animals. The international and public health significance of these pathogens are reviewed comprehensively. The final section is concerned with the host and is organized according to the body system affected. Tables, boxes and flow diagrams provide information in an easily assimilated format. This edition contains new chapters on molecular diagnostics and on infectious conditions of the skin, cardiovascular system, urinary tract and musculoskeletal system. Many new colour diagrams are incorporated into this edition and each chapter has been updated. Key features of this edition: Twelve new chapters included Numerous new illustrations Each chapter has been updated Completely re-designed in full colour Fulfils the needs of veterinary students and academics in veterinary microbiology Companion website with figures from the book as Powerpoints for viewing or downloading by chapter: www.wiley.com/go/quinn/veterinarymicrobiology Veterinary Microbiology and Microbial Disease remains indispensable for all those studying and teaching this essential component of the veterinary curriculum.
Download or read book Parasitic Protozoa of Farm Animals and Pets written by Monica Florin-Christensen and published by Springer. This book was released on 2018-04-17 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an in-depth yet concise overview of the most common and emerging protozoa that cause diseases in both farm animals and companion animals. As outlined in the concise introduction, pathogenic protozoans represent an evolutionary highly diverse and little understood group of disease-causing microorganisms. For each of the featured parasitic unicellular eukaryotes, it discusses the morphology, lifecycle, epidemiology and host-pathogen interactions. In addition, the book highlights the latest developments in diagnostic methods, as well as prevention and treatment strategies. Thorough information on genomes and genetic manipulation strategies for some of the protozoa covered in this book is also included. Infections involving parasitic protozoa can cause productivity losses and/or reduce the quality of life of infected animals. Some infections are zoonotic, posing an on-going public health threat. In most cases, prevention and treatment are either non-existent or need considerable improvement. On the other hand, a great deal of research has recently been conducted on these organisms, yielding valuable new information on their global distribution and revealing the mechanisms of host-pathogen interactions at the molecular level – and essential insights that can be used for the development of new control tools. This book includes extensive information on both basic aspects and recent scientific discoveries on these protozoa and thus constitutes a unique resource for students, veterinarians, and researchers alike.
Download or read book Manual of Standards for Diagnostic Tests and Vaccines written by and published by . This book was released on 2000 with total page 996 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Contaminaci n de Los Productos de Origen Animal written by P. Sutmoller and published by OIE. This book was released on 1997 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Zoonoses E Book written by Joann Colville and published by Elsevier Health Sciences. This book was released on 2007-07-04 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: This essential, authoritative handbook provides clear, accurate coverage of zoonoses — diseases that can spread from animals to humans. The consistent format helps you quickly locate key information, such as how each disease affects the host, how it is spread, how it is treated, and necessary safety precautions. It also discusses the importance of educating animal owners about the public health implications of zoonoses and how to prevent them from spreading. Clear, concise coverage helps you respond quickly when presented with diseases that could potentially spread between patients, clients, and staff in the veterinary clinic. Each disease entry begins with a chart of its potential morbidity (the rate of incidence of a disease) and mortality (death rate), giving you at-a-glance access to the chance of contracting the disease and the severity of the disease if contracted. Clinically relevant coverage includes information on the etiology (bacterial, viral, parasitic, etc.), most common nonhuman hosts, transmission modes, course of the disease, clinical signs in animals and humans, diagnostic tests, prevention, and general advice. Essential information on preventing the spread of disease helps you educate clients about how to protect themselves and their animals from zoonoses. Coverage of diseases such as mad cow disease, West Nile virus, rabies, and anthrax, prepares you to answer client questions about diseases that are in the public eye.
Download or read book Red Book Atlas of Pediatric Infectious Diseases written by American Academy of Pediatrics and published by . This book was released on 2007 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Based on key content from Red Book: 2006 Report of the Committee on Infectious Diseases, 27th Edition, the new Red Bookr Atlas is a useful quick reference tool for the clinical diagnosis and treatment of more than 75 of the most commonly seen pediatric infectious diseases. Includes more than 500 full-color images adjacent to concise diagnostic and treatment guidelines. Essential information on each condition is presented in the precise sequence needed in the clinical setting: Clinical manifestations, Etiology, Epidemiology, Incubation period, Diagnostic tests, Treatment
Download or read book Biofilms in the Food and Beverage Industries written by P. Fratamico and published by CRC Press. This book was released on 2009-10-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biofilms in the Food and Beverage Industries reviews the formation of biofilms and the best practices for their control. When bacteria attach to and colonize the surfaces of food processing equipment and food products themselves, there is a risk that biofilms may form. Human pathogens in biofilms can be harder to remove than free microorganisms and may therefore pose a more significant food safety risk. Part one considers fundamental aspects such as molecular mechanisms of biofilm formation by food-associated bacteria and methods for biofilm imaging, quantification and monitoring. Part two then reviews biofilm formation by different microorganisms. Chapters in Part three focus on significant issues related to biofilm prevention and removal. Contributions on biofilms in particular food industry sectors, such as dairy and red meat processing and fresh produce, complete the collection. With its distinguished editors and international team of contributors, Biofilms in the Food and Beverage Industries is a beneficial reference for microbiologists and those in industry responsible for food safety.
Download or read book Canine and Feline Infectious Diseases E BOOK written by Jane E. Sykes and published by Elsevier Health Sciences. This book was released on 2013-06-06 with total page 928 pages. Available in PDF, EPUB and Kindle. Book excerpt: Canine and Feline Infectious Diseases is a practical, up-to-date resource covering the most important and cutting-edge advances in the field. Presented by a seasoned educator in a concise, highly visual format, this innovative guide keeps you current with the latest advances in this ever-changing field. 80 case studies illustrate the clinical relevance of the major infectious disease chapters. Well-organized Major Infectious Diseases chapters break down content by etiologic agent and epidemiology, clinical signs and their pathophysiology, physical examination findings, diagnosis, treatment and prognosis, immunity, prevention, and public health implications. Over 80 case studies illustrate how the information provided can be applied in everyday practice. Logical approach to laboratory diagnosis guides you through all the steps needed to accurately diagnose and treat viral, bacterial, fungal, protozoal, and algal diseases. Practical protocols provided by expert clinicians guide you in the management of canine and feline patients suspected to have infectious diseases, including handling, disinfection, isolation, and vaccination protocols. Over 500 full color images – geographic distribution maps, life cycle drawings, and hundreds of color photographs – visually illustrate and clarify complex issues. Easy-to-understand tables and boxes make content quickly accessible, eliminating the need to sort through dense text for critical information in the clinical setting.
Download or read book Skin and Arthropod Vectors written by Nathalie Boulanger and published by Academic Press. This book was released on 2018-01-20 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent research on skin immunity and the skin microbiome reveals the complexity of the skin and its importance in the development of immunity against arthropod-borne diseases. In diseases such as malaria, borreliosis, leishmaniasis, trypanosomiasis, etc., the skin interface has been shown as an essential site for pathogens to hide from the immune system, and as a potential site of persistence. Only very few vaccines have been successfully developed so far against these diseases, likely because of an insufficient understanding on the development of skin immunity against pathogens. Skin and Arthropod Vectors expands our knowledge on the role of the skin interface during the transmission of arthropod-borne diseases and particularly its immunity. This work may support researchers who strive for developing more efficient diagnostic tools and vaccines. It also gives scientists and advanced students working in related areas a better insight on how humans and animals are attractive to arthropods to develop better repellents, or to set up transgenic arthropods. Offers the only compilation of research focusing on both the skin interface and arthropod vectors, with contributions from international experts Advances research in the effort toward generating more effective diagnostic tools and vaccines focusing on the skin interface Can also serve as supplemental material for dermatology lectures or specialized lectures on medical entomology and skin immunity
Download or read book Avian Influenza Virus written by Erica Spackman and published by Springer Science & Business Media. This book was released on 2008-02-28 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the growing global fear of a major pandemic, avian influenza (AI) virus research has greatly increased in importance. In Avian Influenza Virus, an expert team of researchers and diagnosticians examine the fundamental, yet essential, virological methods for AI virus research and diagnostics as well as some of the newest molecular procedures currently used for basic and applied research. They present exciting, cutting-edge new methods that focus both on studying the virus itself and on work with avian hosts, an area greatly lacking in research.
Download or read book The Molecular Identification of Neospora Sp Parasites in Bovine and Nonhuman Primate Dams and Fetuses written by Michael Shiu-Yan Ho and published by . This book was released on 1996 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt:
