Download or read book Designing Cell responsive Hydrogels for Bioactive Tissue Engineering Constructs written by Derek R Jones and published by . This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Long-term viability of three-dimensional tissue engineered constructs remains a major hurdle due to challenges associated with mass transfer of oxygen, nutrients, and waste products. To address this shortcoming, this work focuses on designing cell-responsive materials to be utilized in a bioactive tissue engineered construct, with a particular emphasis on vasculogenesis. We hypothesized that highly tunable synthetic materials tailored with the appropriate bioactivity could allow for: cell attachment, cell mediated degradation, and growth factor release in vitro. To accomplish this, bio-inert PEG hydrogels were used as a scaffold substrate. To mimic the properties of the extracellular matrix (ECM), cell-adhesive peptide (CGRGDSP), enzyme-sensitive peptide (CGPQGIAGQC), and bi-functional peptide (CGPQGIAGQCGRGDSP) were incorporated into the hydrogel scaffold. This, in conjunction with multi-arm PEG acrylate and Michael Addition chemistry, allowed for the synthesis of bio-active hydrogels that could be synthesized free of cytotoxic photoinitiator, and had decoupled hydrogel properties for more appropriate comparison between formulations. The resulting hydrogels could bind cells as a function of cell adhesive peptide concentration and degrade as a function of collagenase. Separately, enzymatically degradable growth factor loaded nanoparticles were synthesized using a liposome template technique in conjunction with dehydration-rehydration loading. This allowed for a high concentration of growth factor to be loaded into nanoparticles, while simultaneously preventing the deactiviation of growth factor through extreme processing conditions. Moreover, through the incorporation of enzymatically degradable peptide (VPMSMRGG) into the backbone of these hydrogel nanoparticles, we alter the release profile of the growth factor both as a function of collagenase concentration as well as cell concentration. These bioactive constructs offer the increased ability for cells to manipulate their microenvironment, and show promise as materials to be used in vasculogenic tissue engineered constructs.
Download or read book Tunable Hydrogels written by Antonina Lavrentieva and published by Springer Nature. This book was released on 2021-05-31 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the current knowledge on tunable hydrogels, including the range of different materials and applications, as well as the existing challenges and limitations in the field. It covers various aspects of the material design, particularly highlighting biological responsiveness, degradability and responsiveness to external stimuli. In this book, readers will discover original research data and state-of-the-art reviews in the area of hydrogel technology, with a specific focus on biotechnology and medicine. Written by leading experts, the contributions outline strategies for designing tunable hydrogels and offer a detailed evaluation of the physical and synthetic methods currently employed to achieve specific hydrogel properties and responsiveness. This highly informative book provides important theoretical and practical insights for scholars and researchers working with hydrogels for biomedical and biotechnological applications.
Download or read book Hydrogels written by Thakur Raghu Raj Singh and published by CRC Press. This book was released on 2018-02-28 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hydrogels are crosslinked, macromolecular polymeric materials arranged in a three-dimensional network, which can absorb and retain large amounts of water. Hydrogels are commonly used in clinical practice and experimental medicine for a wide range of applications, including drug delivery, tissue engineering and regenerative medicine, diagnostics, cellular immobilization, separation of biomolecules or cells, and barrier materials to regulate biological adhesions. This book elucidates the underlying concepts and emerging applications of hydrogels and will provide key case studies and critical analysis of the existing research.
Download or read book Hydrogels in Tissue Engineering written by Esmaiel Jabbari and published by MDPI. This book was released on 2018-08-24 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue " Hydrogels in Tissue Engineering" that was published in Gels
Download or read book Multifunctional Hydrogels for Biomedical Applications written by Ricardo A. Pires and published by John Wiley & Sons. This book was released on 2022-05-31 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multifunctional Hydrogels for Biomedical Applications Comprehensive resource presenting a thorough overview of the biomedical applications of hydrogels This book provides an overview of the development and applications of the clinically relevant hydrogels that are used particularly in tissue engineering, regenerative medicine, and drug delivery. Taking a multidisciplinary approach, it goes through the material from chemistry, materials science, biology, medicine, nanotechnology, and bioengineering points of view. Sample topics covered by the three well-qualified editors include: The design, functions, and developments of hydrogels Proteins and polysaccharides that mimic extracellular matrix Generation and applications of supramolecular hydrogels Design and functions of cell encapsulation systems Multifunctional Hydrogels for Biomedical Applications is a useful all-in-one reference work for materials scientists, polymer chemists, and bioengineers which provides a comprehensive, contemporary understanding of hydrogels and their applications targeting a wide variety of pathologies.
Download or read book Bone Tissue Engineering written by Jeffrey O. Hollinger and published by CRC Press. This book was released on 2004-10-14 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focusing on bone biology, Bone Tissue Engineering integrates basic sciences with tissue engineering. It includes contributions from world-renowned researchers and clinicians who discuss key topics such as different models and approaches to bone tissue engineering, as well as exciting clinical applications for patients. Divided into four sections, t
Download or read book Injectable Hydrogels for 3D Bioprinting written by Insup Noh and published by Royal Society of Chemistry. This book was released on 2021-07-30 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hydrogels represent one of the cornerstones in tissue engineering and regenerative medicine, due to their biocompatibility and physiologically relevant properties. These inherent characteristics mean that they can be widely exploited as bioinks in 3D bioprinting for tissue engineering applications as well as injectable gels for cell therapy and drug delivery purposes. The research in these fields is booming and this book provides the reader with a terrific introduction to the burgeoning field of injectable hydrogel design, bioprinting and tissue engineering. Edited by three leaders in the field, users of this book will learn about different classes of hydrogels, properties and synthesis strategies to produce bioinks. A section devoted to the key processing and design challenges at the hydrogel/3D bioprinting/tissue interface is also covered. The final section of the book closes with pertinent clinical applications. Tightly edited, the reader will find this book to be a coherent resource to learn from. It will appeal to those working across biomaterials science, chemical and biomedical engineering, tissue engineering and regenerative medicine.
Download or read book Polymeric Biomaterials for Tissue Regeneration written by Changyou Gao and published by Springer Nature. This book was released on 2024-02-01 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews state of the art of polymeric biomaterials for regenerative medicine and highlights advances in both fundamental science and clinical practice. It summarizes the latest techniques in polymeric scaffold fabrication, delivery carriers, physiochemical property modulation, as well as their influence on the adhesion and performance of biomolecules, cells, and tissues. It also describes methods for creating biofunctional surfaces/interfaces and subsequently modulating the host response to implantable materials. Lastly, it discusses the applications of biomaterials and constructs in soft-tissue regenerative medicine. It is a valuable resource for materials scientists and engineers wishing to identify research priorities to fulfill clinical needs and provides physicians with insights into emerging novel biomaterials. This integrated approach also offers engineering students a sense of the relevance of materials science in the development of novel therapeutic strategies. In the second edition, most of the chapters are updated according to the latest progress of this research field. A new chapter on nerve regeneration is also included.
Download or read book Design and Fabrication of Hydrogels for Tissue Engineering Applications written by Hossein Ravanbakhsh Habibabadi and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "Many single-phase hydrogels offer limited cell-material interaction due to their lack of focal adhesion sites. Single-phase hydrogels generally cannot mimic the heterogeneous structure and composition of the extracellular matrix (ECM), resulting in an entirely different cell mechanobiological behavior in three-dimensional (3D) cell culture. Furthermore, single-phase hydrogels may lack sufficient mechanical properties to resemble the stiffness of the target organs. Composite hydrogels, which are made of a mixture of single-phase hydrogel and various additives, can address the above shortcomings. With this aim, the composition of single-phase glycol chitosan (GC) hydrogel was modified by introducing carbon nanotube (CNT) as an additive. The incorporation of CNTs results in a fibrous structure, which mimics many human tissues, such as vocal fold lamina propria. It was hypothesized that CNTs may affect the properties of the hydrogel scaffold and its interaction with the encapsulated cells. Specifically, as CNTs can provide focal adhesion sites for the cells, CNT-loaded hydrogels were postulated to promote cell recruitment and cell migration.Porous composite hydrogels were prepared using GC as the matrix, glyoxal as the chemical crosslinker, and CNTs as the additive. Both carboxylic acid-functionalized carbon nanotubes (COOH-CNTs) and hydroxyl-functionalized carbon nanotubes (OH-CNTs) were used for fabricating composite hydrogels. The homogeneity of CNT dispersion was evaluated using scanning electron microscopy (SEM). Human vocal fold fibroblast (HVFF) cells were cultured and encapsulated in the composite hydrogels with different CNT concentrations to quantify cell viability. Rheological tests were performed to determine the gelation time and the storage modulus as a function of CNT concentration.Ideally, an injectable hydrogel implant should promote the recruitment of cells from the surrounding native tissue and allow cells to migrate freely as they generate a new ECM network. Carbon nanotube glycol chitosan (CNT-GC) hydrogels were tested to investigate this phenomenon. Chemoattractant-induced cell migration was studied using a modified Boyden Chamber experiment. Migrated cells were counted using flow cytometry. Cell adhesion was inferred from the morphology of the cells via an image segmentation method. The results confirmed that CNTs significantly impact cell-material interaction in hydrogels by enhancing cell adhesion, cell migration, and cell recruitment.The cryobioprinting performance was investigated by designing, fabricating, and storing cell-laden constructs made of cryoprotective bioinks using a freezing plate with precisely controllable temperature. The in situ freezing process further promoted the printability of cell-laden hydrogel bioinks to achieve freeform structures otherwise difficult to obtain with direct extrusion bioprinting. The effects of bioink components on printability and cell viability were evaluated. The functionality of the method was finally investigated using cell differentiation and chick ex ovo assays. The results demonstrated the feasibility and efficacy of cryobioprinting as a single-step method for concurrent hydrogel-based tissue biofabrication and storage.The findings in this study showed that low concentrations of CNTs did not cause significant cytotoxicity in hydrogel scaffolds. In such concentrations, CNTs promoted cell migration and cell adhesion, as verified using modified Boyden Chamber experiments. Cryobioprinting, as an approach for enhancing the performance of hydrogels, was found to be an effective technique that synergistically exploits the advantages of bioprinting and cryopreservation methods. It was shown that storable tissue constructs can be fabricated using the cryobioprinting method. Overall, the novel concept presented in this dissertation holds promise for a more practical application of hydrogels in tissue engineering (TE)"--
Download or read book Biomedical Hydrogels written by Steve Rimmer and published by Elsevier. This book was released on 2011-02-26 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hydrogels are very important for biomedical applications because they can be chemically manipulated to alter and control the hydrogel’s interaction with cells and tissues. Their flexibility and high water content is similar to that of natural tissue, making them extremely suitable for biomaterials applications. Biomedical hydrogels explores the diverse range and use of hydrogels, focusing on processing methods and novel applications in the field of implants and prostheses. Part one of this book concentrates on the processing of hydrogels, covering hydrogel swelling behaviour, superabsorbent cellulose-based hydrogels and regulation of novel hydrogel products, as well as chapters focusing on the structure and properties of hydrogels and different fabrication technologies. Part two covers existing and novel applications of hydrogels, including chapters on spinal disc and cartilage replacement implants, hydrogels for ophthalmic prostheses and hydrogels for wound healing applications. The role of hydrogels in imaging implants in situ is also discussed. With its distinguished editor and international team of contributors, Biomedical hydrogels is an excellent reference for biomedical research scientists and engineers in industry and academia, as well as others involved in research in this area, such as research clinicians. Examines the diverse range and use of hydrogels, focusing on processing methods and novel applications Comprehensive book explores the structure and properties of hydrogels and different fabrication technologies Covers important areas such as processing of hydrogels, covering hydrogel swelling behaviour, superabsorbent cellulose-based hydrogels and regulation of novel hydrogel products
Download or read book Gels Handbook Fundamentals Properties Applications In 3 Volumes written by and published by World Scientific. This book was released on 2016-01-25 with total page 1170 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hydrogels are made from a three-dimensional network of cross linked hydrophilic polymers or colloidal particles that contain a large fraction of water. In recent years, hydrogels have attracted significant attention for a variety of applications in biology and medicine. This has resulted in significant advances in the design and engineering of hydrogels to meet the needs of these applications. This handbook explores significant development of hydrogels from characterization and applications. Volume 1 covers state-of-art knowledge and techniques of fundamental aspects of hydrogel physics and chemistry with an eye on bioengineering applications. Volume 2 explores the use of hydrogels in the interdisciplinary field of tissue engineering. Lastly volume 3 focuses on two important aspects of hydrogels, that is, drug delivery and biosensing. Contains 50 colour pages.
Download or read book Gels Handbook written by Utkan Demirci and published by World Scientific Publishing Company. This book was released on 2016-01-09 with total page 1000 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hydrogels are made from a three-dimensional network of cross linked hydrophilic polymers or colloidal particles that contain a large fraction of water. In recent years, hydrogels have attracted significant attention for a variety of applications in biology and medicine. This has resulted in significant advances in the design and engineering of hydrogels to meet the needs of these applications. This handbook explores significant development of hydrogels from characterization and applications. Volume 1 covers state-of-art knowledge and techniques of fundamental aspects of hydrogel physics and chemistry with an eye on bioengineering applications. Volume 2 explores the use of hydrogels in the interdisciplinary field of tissue engineering. Lastly volume 3 focuses on two important aspects of hydrogels, that is, drug delivery and biosensing. Contains 50 colour pages.
Download or read book Biomedical Applications of Hydrogels Handbook written by Raphael M. Ottenbrite and published by Springer Science & Business Media. This book was released on 2010-09-05 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hydrogels are networks of polymer chains which can produce a colloidal gel containing over 99 per cent water. The superabsorbency and permeability of naturally occurring and synthetic hydrogels give this class of materials an amazing array of uses. These uses range from wound dressings and skin grafts to oxygen-permeable contact lenses to biodegradable delivery systems for drugs or pesticides and scaffolds for tissue engineering and regenerative medicine. Biomedical Applications of Hydrogels Handbook provides a comprehensive description of this diverse class of materials, covering both synthesis and properties and a broad range of research and commercial applications. The Handbook is divided into four sections: Stimuli-Sensitive Hydrogels, Hydrogels for Drug Delivery, Hydrogels for Tissue Engineering, and Hydrogels with Unique Properties. Key Features: Provides comprehensive coverage of the basic science and applications of a diverse class of materials Includes both naturally occurring and synthetic hydrogels Edited and written by world leaders in the field.
Download or read book Engineered Tissues Using Bioactive Hydrogels written by Di Huang and published by Frontiers Media SA. This book was released on 2022-08-22 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nano Hydrogels written by Jiya Jose and published by Springer Nature. This book was released on 2021-01-04 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces the reader to important aspects of the nano-hydrogels. It covers the development of hydrogels and their biology, chemistry and properties. Focus is also given to innovative characterization techniques and advances in structural design, with special emphasis on molecular structure, dynamic behavior and structural modifications of hydrogels. This book serves as a consolidated reference work for the diverse aspects of hydrogels, creating a valuable resource for students and researchers in academia and industry.
Download or read book Scaffolding In Tissue Engineering written by Peter X. Ma and published by CRC Press. This book was released on 2005-08-19 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt: The growing interest in scaffolding design and increasing research programs dedicated to regenerative medicine corroborate the need for Scaffolding in Tissue Engineering. While certain books and journal articles address various aspects in the field, this is the first current, comprehensive text focusing on scaffolding for tissue engineering.
Download or read book Development of Environment Responsive Hydrogels for the Delivery of Therapeutic Agents written by Junbin Shi and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
