Download or read book Cryopreservation of Plant Germplasm I written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt: The germ plasm of numerous plant species, especially those of forest trees, some agricultural crops, and medicinal plants, is endangered and threatened with extinction. This depletion of germplasm pools and the shrinkage of naturally occurring genetic resources have caused international concern. Conventionally, the germplasm of plants is conserved through seeds, tubers, roots, corms, rhizomes, bulbs, cuttings, etc. However, the germ plasm of a number of trees and plantation crops (such as coconut, cocao, coffee, oil palm, rubber, mango, horse chestnut, etc. ) cannot be preserved since their seed are short-lived (recalcitrant). Likewise, germplasm of vegetatively propagated crops (such as potato and cassava) cannot be stored on a long term basis and has to be grown and multiplied periodically in nurseries and fields. The plants are thus exposed to unpredictable weather conditions and diseases, with the result that instances are known where entire genetic stocks are lost. Therefore, unconventional methods are being developed for the storage and international exchange of germplasm. For this purpose in vitro cultures have been employed, but they can only enable short-to medium term preservation; moreover, cell cultures upon repeated subculture undergo genetic erosion. In view of the recent developments in the in vitro induction of genetic variability through somaclonal variation, somatic hybridization, recombinant DNA technology, etc. , new methods need to be employed for the storage of desirable cultures. In this regard freeze preservation of cells in liquid nitrogen (-196 0q, like that of semen, enables long-term storage, theoretically, for an indefinite period of time.
Download or read book Cryopreservation of Plant Germplasm I written by Y. P. S. Bajaj and published by Springer. This book was released on 1995-09-29 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt: The germ plasm of numerous plant species, especially those of forest trees, some agricultural crops, and medicinal plants, is endangered and threatened with extinction. This depletion of germplasm pools and the shrinkage of naturally occurring genetic resources have caused international concern. Conventionally, the germplasm of plants is conserved through seeds, tubers, roots, corms, rhizomes, bulbs, cuttings, etc. However, the germ plasm of a number of trees and plantation crops (such as coconut, cocao, coffee, oil palm, rubber, mango, horse chestnut, etc. ) cannot be preserved since their seed are short-lived (recalcitrant). Likewise, germplasm of vegetatively propagated crops (such as potato and cassava) cannot be stored on a long term basis and has to be grown and multiplied periodically in nurseries and fields. The plants are thus exposed to unpredictable weather conditions and diseases, with the result that instances are known where entire genetic stocks are lost. Therefore, unconventional methods are being developed for the storage and international exchange of germplasm. For this purpose in vitro cultures have been employed, but they can only enable short-to medium term preservation; moreover, cell cultures upon repeated subculture undergo genetic erosion. In view of the recent developments in the in vitro induction of genetic variability through somaclonal variation, somatic hybridization, recombinant DNA technology, etc. , new methods need to be employed for the storage of desirable cultures. In this regard freeze preservation of cells in liquid nitrogen (-196 0q, like that of semen, enables long-term storage, theoretically, for an indefinite period of time.
Download or read book Cryopreservation of Plant Germplasm II written by L.E. Towill and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ex situ preservation of germplasm for higher plant species has been accom plished using either seeds or clones, but storage of these under typical condi tions does not provide the extreme longevities that are needed to minimize risk of loss. Costs of maintenance and regeneration of stocks are also high. Systems that provide virtually indefinite storage should supplement existing methods and it is within this context that cryopreservation is presented. The use of low temperature preservation was initially more a concern of medicine and animal breeding, and was expanded to plants in the 1970s. Sur vival after cryogenic exposure has now been demonstrated for diverse plant groups including algae, bryophytes, fungi and higher plants. If survival is com monplace, then the eventual application is a cryopreservation system, whereby cells, tissues and organs are held indefinitely for use, often in the unforeseen future. The increasing interest and capabilities for application could not have occurred at a more opportune time since expanding human populations have placed unprecedented pressures on plant diversity. This book emphasizes cry opreservation of higher plants and was initially driven by the concern for loss of diversity in crops and the recognized need that this diversity would be essential for continued improvement of the many plants used by society for food, health and shelter. The interest in cryopreservation has been expanded by conservationists and their concerns for retaining, as much as possible, the diversity of natural populations. The need for cryopreservation, thus, is well established.
Download or read book Plant Cryopreservation A Practical Guide written by Barbara B.M. Reed and published by Springer Science & Business Media. This book was released on 2007-10-31 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryopreservation has proven to be an important tool for the storage and conservation of plant genetic resources. This book is a unique resource for plant scientists, providing more than 100 ready-to-use cryopreservation protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems and for cell types such as suspension and callus cultures.
Download or read book Cryopreservation of Plant Germplasm written by and published by . This book was released on 1995 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Managing Global Genetic Resources written by National Research Council and published by National Academies Press. This book was released on 1993-02-01 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: This anchor volume to the series Managing Global Genetic Resources examines the structure that underlies efforts to preserve genetic material, including the worldwide network of genetic collections; the role of biotechnology; and a host of issues that surround management and use. Among the topics explored are in situ versus ex situ conservation, management of very large collections of genetic material, problems of quarantine, the controversy over ownership or copyright of genetic material, and more.
Download or read book Plant Cryopreservation written by Carla Benelli and published by Mdpi AG. This book was released on 2022-01-05 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryopreservation is the storage of cells, tissues and organs at ultra-low temperatures (-196 °C) and can be an important tool for long-term conservation of plant genetic resources. A wide range of cryogenic procedures have been developed for in vitro explants, dormant buds and non-orthodox seeds in many plant species; however, research that provides novelty, insight and advances for developing and improving new protocols in plant species is still needed. This book includes the development of new protocols, physical and chemical studies related to preservation in liquid nitrogen, and some reviews on several aspects of cryopreservation. This publication will be a useful document for the community of cryopreservation scientists.
Download or read book Cryopreservation of Tropical Plant Germplasm written by Florent Engelmann and published by . This book was released on 2000 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Development of cryopreservation techniques. Importance of cryopreservation for the conservation of plant genetic resources. Fundamental aspects of cryopreservation. Cryopreservation techniques. Ongoing cryopreservation projects -- Research and its application. Current status of cryopreservation research and future perspectives of its application in national programmes.
Download or read book Cryopreservation of Plant Germplasm I written by Y. P. S. Bajaj and published by Springer Verlag. This book was released on 1995 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Whole plants have been regenerated from isolated protoplasts, cells, tissues, and organs freeze-preserved in liquid nitrogen. Thus cryopreservation has proved to be an important tool for the storage and conservation of plant genetic resources. Numerous experts have contributed to this volume, which is divided into the following sections: General introduction into the cryopreservation of plant cells, tissues, organs and seeds; trees; cereals and grasses; legumes and oilseed crops; fruits, vegetables, tuber and root crops; medicinal, aromatic and ornamental plants.
Download or read book Cryopreservation of Musa Germplasm written by Bart Panis and published by Bioversity International. This book was released on 2001 with total page 45 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until 10 years ago, cryopreservation protocols for plant tissues were mainly based on slow freezing in the presence of cryoprotective mixtures containing DMSO (dimthyl slphoxide), sugars, glycerol and/ or proline. Slow freezing results in a freeze-dehydration, leaving less water in the cells to form lethal ice crystals upon exposure to extreme low temperatures.
Download or read book Cryopreservation of Musa Germplasm written by Bart Panis and published by Bioversity International. This book was released on 2001-12-01 with total page 45 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Applied and Fundamental Aspects of Plant Cell Tissue and Organ Culture written by Jakob Reinert and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 810 pages. Available in PDF, EPUB and Kindle. Book excerpt: Progress in the field of plant cell and tissue culture has made this area of research one of the most dynamic and promising not only in plant physiology, cell biology and genetics but also in agriculture, forestry, horticulture and industry. Studies with plant cell cultures clearly have bearing upon a variety of problems as yet unsolved in basic and applied research. This was the compelling reason for assembling such a comprehensive source of information to stimulate students, teachers, and research workers. This book comprises 34 articles on regeneration of plants, vegetative propagation and cloning; haploids; cytology, cytogenetics and plant breeding; protoplasts, somatic hybridization and genetic engineering; plant pathology; secondary products and a chapter on isoenzymes, radiobiology, and cryobiology of plant cells. Particular attention has been paid to modern , fast-growing and fascinating disciplines - e.g. the induction of haploids, somatic hybridization and genetic manipulation by protoplast culture, which possess an enormous potential for plant improvement.
Download or read book Conservation of Plant Genetic Resources in Vitro General aspects written by M. K. Razdan and published by Bib. Orton IICA / CATIE. This book was released on 1997 with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text presents the research findings of international research institutes and organizations who have co-ordinated efforts to use tissue culture methods for the conservation of certain crops. The articles highlight the direction of efforts in germplasm conservation.
Download or read book Cryopreservation of Tropical Plant Germplasm written by F. Engelmann and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Development of cryopreservation techniques. Importance of cryopreservation for the conservation of plant genetic resources. Fundamental aspects of cryopreservation. Cryopreservation techniques. Ongoing cryopreservation projects -- Research and its application. Current status of cryopreservation research and future perspectives of its application in national programmes.
Download or read book Plant Cell Biotechnology written by M. Salome S. Pais and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 493 pages. Available in PDF, EPUB and Kindle. Book excerpt: A number of interdisciplinary fields related to Plant Cell Biotechnology are discussed. The two main directions are: Plant cell culture in agricultural applications for the improvement of crops and industrial applications in the production of secondary metabolites. A number of areas such as physiological and biochemical aspects of autotrophic cells, gene characterization in higher plants, transformation of plant cells, genetic stability in plant cell cultures, somatic hybridization and somatic embryogenesis are treated. Recent knowledge on somaclonal and gametoclonal variation as well as on the obtainment of protoplasts and their use for the isolation and culture of heterocaryons as tools for plant breeding are considered. Furthermore, the knowledge on biomass production in fermentor conditions and the role of immobilization for increased production and scale-up of plant cells are discussed.
Download or read book Technical Guidelines for the Management of Field and in Vitro Germplasm Collections written by B. M. Reed and published by Bioversity International. This book was released on 2004 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Preservation of Plant Cells in Vitro written by Brian Grout and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: The long-term storage and maintenance of viable plant cells and organs is an area of active concern across the range of pure and applied plant sciences. In academic, government and commercial laboratories, the extended storage of propagules of one sort or another, with maximum protection of the genome from mutation and altered expression, is often a very necessary activity that can draw heavily on resources and effort. However, preservation per se is typically not an activity in its own right, but a facilitating technology that is part of a larger programme of work. Consequently, there are many laboratories that do not have the benefit of a specialist in storage technology, and have to delegate the responsibility to individuals, or teams, who are faced with a daunting learning curve. To maximise the chances of success, in the shortest possible time and with minimum losses, these researchers need sources of reference that are au thoritative and soundly based in practical experience.
