Download or read book Constructions of 2D 3D Bio mimetic DNA Nanostructures written by Sibai Xie and published by . This book was released on 2021 with total page 83 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA nanotechnology as a versatile tool has been growing rapidly in the past three decades, with applications across different disciplines such as drug delivery, imaging, and even computing. Its versatility stems from the programmable nature of DNA and the ability to self-assemble through the A-T and C-G base pairing by hydrogen bonds. These features make DNA nanotechnology the ideal tool for mimicking and studying biological processes that are hard to otherwise model. In this work, the process we focus on is Clathrin-Mediated Endocytosis (CME). CME is one of the major mechanisms for cell entry. This pathway internalizes Influenza A, vesicular stomatitis virus, and many others. Mimicking this process will help us further understand it, and also find ways to utilize it in the field of drug delivery. The process of CME can be described in 3 major steps. The first step is array formation. Clathrin can cluster on cell surface in the form of triskelion, to form small patches of an array. The second step involves the recruitment of adaptor proteins, that transforms the array into three-dimensional lattices to encapsulate the foreign agent. The third step is the transportation of the vehicle inside the cell, along with the disassembly of the vehicle to release the agent. Specifically for each stage, we designed mimicking mechanisms using DNA nano-structures. For the array stage, we designed a three-point-star motif to mimic the triskelion structure, and functionalized it with cholesterol to integrate the array to the cell membrane. Characterization through liquid atomic force microscopy (AFM) showed clear hexagonal pattern, and in vitro cell experiment also showed the integration of the arrays to cell membranes. For the transition stage, we designed a reversible 2D-3D transition mechanism that allowed the 2D arrays to transform into 3D particles with the addition of a particular stimuli, that is a DNA single strand. The transformability and reversibility were confirmed through polyacrylamide gel electrophoresis (PAGE), dynamic light scattering (DLS) and AFM. For the delivery stage, we designed several 3D DNA structures for better drug delivery efficiency, and the designs were also characterized by PAGE and AFM. The successful designs for all three stages led us closer to understanding the structural transformation of clathrin triskelion during CME. The 2D-3D transition mechanism also has the potential to be used in other systems, such as stimuli-controlled drug release and DNA computing using single strand DNA.
Download or read book Structural DNA Nanotechnology written by Nadrian C. Seeman and published by Cambridge University Press. This book was released on 2015 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the founder of the field, this is a comprehensive and accessible introduction to structural DNA nanotechnology.
Download or read book Fundamentals of Bionanomaterials written by Ahmed Barhoum and published by Elsevier. This book was released on 2022-01-26 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bionanomaterials are molecular materials composed partially or completely of biological molecules, key biological structures, such as proteins, enzymes, viruses, DNA, biopolymers as well as metal, metal oxides, and carbon nanomaterials with characteristic bioactivity. Bionanomaterials have drawn much attention for their use in a wide range of industrial applications from scaffolds, dental implants, drug delivery, dialysis, biobatteries, biofuel cells, air purification, and water treatment. Therefore, the intensive current research in this area is driven towards the designing and functionalization of bionanomaterials for industrial applications. Fundamentals of Bionanomaterials covers the fundamental aspects, experimental setup, synthesis, properties, and characterization of the different types of bionanomaterial. It discusses the different structure and unique properties of bionanomaterials that can be obtained by modifying their morphology and composition, highlighting a wide range of fabrication techniques of bionanomaterials and critical processing parameters. This is an important reference source for all those seeking to gain a solid understanding of the characterization, properties and processing a variety of bionanomaterial classes. Explains the major properties and characterization techniques for a range of bionanomaterial classes Discusses the commercialization of different types of bionanomaterials for a variety of industry sectors Highlights the challenges and interdisciplinary perspective of bionanomaterials in science, biology, engineering, medicine, and technology, incorporating both fundamentals and applications
Download or read book DNA and RNA Origami written by Julián Valero and published by Springer Nature. This book was released on 2023-05-11 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume detials diverse methodological approaches on the assembly and applications of DNA origami assemblies. Chapters guide readers through different synthetic and computational methods, isolation and structural characterization of 2D and 3D DNA origami nanoarchitectures, nanophotonics, drug delivery, biophysics, and synthetic biology.Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA and RNA Origami: Methods and Protocols aims to serve as a guideline describing the current state-of-the-art assembly methodologies and applications of DNA origami nanostructures.
Download or read book Nanopharmaceuticals Principles and Applications Vol 1 written by Vinod Kumar Yata and published by Springer Nature. This book was released on 2020-07-14 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the biological, technical and study-design challenges of Nanopharmaceuticals. Chapters of this book are dedicated to supermagentic iron oxide nanoparticles for the diagnosis of brain, breast, gastric, ovarian, liver, colorectal, lung and pancreatic cancers. It also includes a brief introduction to magnetic resonance imaging and ends with the future prospective of iron oxide nanoparticles in cancer detection. The book also provides a critical discussion on ‘Computational sequence design for DNA nanostructures’ and gives a brief introduction about the skin delivery. A detailed discussion has been included about the different types of nanocarriers such as micells, microemulsions, nanoemulsions, polymeric and lipid based nanoparticles. Focussing on the safety concerns of nanomedicine it also covers the safety issues, clinical benefits, ecotoxicity and regulatory frame work of nanopharmaceuticals.
Download or read book DNA Nanotechnology written by Chunhai Fan and published by Springer Nature. This book was released on 2020-09-07 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: The series Topics in Current Chemistry Collections presents critical reviews from the journal Topics in Current Chemistry organized in topical volumes. The scope of coverage is all areas of chemical science including the interfaces with related disciplines such as biology, medicine and materials science. The goal of each thematic volume is to give the non-specialist reader, whether in academia or industry, a comprehensive insight into an area where new research is emerging which is of interest to a larger scientific audience. Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field. The chapter "DNA-Programmed Chemical Synthesis of Polymers and Inorganic Nanomaterials" is available open access under a CC BY 4.0 License via link.springer.com.
Download or read book Protein Self Assembly written by Jennifer J. McManus and published by Humana. This book was released on 2020-08-08 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
Download or read book DNA Origami written by Masayuki Endo and published by John Wiley & Sons. This book was released on 2022-05-10 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA ORIGAMI Discover the impact and multidisciplinary applications of this subfield of DNA nanotechnology DNA origami refers to the technique of assembling single-stranded DNA template molecules into target two- and three-dimensional shapes at the nanoscale. This is accomplished by annealing templates with hundreds of DNA strands and then binding them through the specific base-pairing of complementary bases. The inherent properties of these DNA molecules—molecular recognition, self-assembly, programmability, and structural predictability—has given rise to intriguing applications from drug delivery systems to uses in circuitry in plasmonic devices. The first book to examine this important subfield, DNA Origami brings together leading experts from all fields to explain the current state and future directions of this cutting-edge avenue of study. The book begins by providing a detailed examination of structural design and assembly systems and their applications. As DNA origami technology is growing in popularity in the disciplines of chemistry, materials science, physics, biophysics, biology, and medicine, interdisciplinary studies are classified and discussed in detail. In particular, the book focuses on DNA origami used for creating new functional materials (combining chemistry and materials science; DNA origami for single-molecule analysis and measurements (as applied in physics and biophysics); and DNA origami for biological detection, diagnosis and therapeutics (medical and biological applications). DNA Origami readers will also find: A complete guide for newcomers that brings together fundamental and developmental aspects of DNA origami technology Contributions by a leading team of experts that bring expert views from different angles of the structural developments and applications of DNA origami An emerging and impactful research topic that will be of interest in numerous multidisciplinary areas A helpful list of references provided at the end of each chapter to give avenues for further study Given the wide scope found in this groundbreaking work, DNA Origami is a perfect resource for nanotechnologists, biologists, biophysicists, chemists, materials scientists, medical scientists, and pharmaceutical researchers.
Download or read book Biomimetic Microengineering written by Hyun Jung Kim and published by CRC Press. This book was released on 2020-01-16 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will examine the relevant biological subjects involved in biomimetic microengineering as well as the design and implementation methods of such engineered microdevices. Physiological topics covered include regeneration of complex responses of our body on a cellular, tissue, organ, and inter-organ level. Technological concepts in cell and tissue engineering, stem cell biology, microbiology, biomechanics, materials science, micro- and nanotechnology, and synthetic biology are highlighted to increase understanding of the transdisciplinary methods used to create the more complex, robust biomimetic engineered models. The effectiveness of the new bioinspired microphysiological systems as replacements for existing in vitro or in vivo models is explained through sections that include the protocols to reconstitute three-dimensional (3D) structures, recapitulate physiological functions, and emulate the pathophysiology of human diseases. This book will also discuss how researchers can discover bridge technologies for disease modeling and personalized precision medicine. Features Focuses on cutting edge technologies that enable manipulation of living systems in a spatiotemporal manner. Incorporates research on reverse engineering of comples microenvironmental factors in human diseases. Highlights technologies related to patient-specific personalized medicine and their potential uses. Written by chapter authors who are highly respected researchers in science and engineering. Includes extensive references at the end of each chapter to enhance further study. Hyun Jung Kim is an Assistant Professor in the Department of Biomedical Engineering at The University of Texas at Austin. After receiving hois Ph.D. degree at Yonsei University in the Republic of Korea, he did extensive postdctoral research at both the University of Chicago and the Wyss Institute at Harvard University. These efforts resulted in cutting-edge breakthroughs in synthetic microbial community research and organomimetic human Gut-on-a-Chip microsystem. His research on Gut-on-a-Chip technology leads to the creation of a microfluidic device that mimics the physiology and pathology of the living human intestine. Since 2015, he has explored novel human host-microbiome ecosystems to discover the disease mechanism and new therapeutics in inflammatory bowel disease and colorectal cancers at UT Austin. In collaboration with clinicians, his lab is currently developing disease-oriented, patient-specific models for the advancement in pharmaceutical and clinical fields.
Download or read book Magnetic Structures of 2D and 3D Nanoparticles written by Jean-Claude Serge Levy and published by CRC Press. This book was released on 2018-09-03 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt: Magnetic nanoparticles appear naturally in rock magnetism together with a large distribution of sizes and shapes. They have numerous applications from nano-size magnetic memories to metamaterials for electromagnetic waves as well as biological applications such as nanosurgery with minimal traumatism. Their long-ranged size- and shape-dependent dipolar interactions provide numerous useful properties. This book describes the preparation as well as the magnetic properties of nanoparticles and also considers 2D dots, nearly spherical samples, elongated samples, and various assemblies of nanoparticles. The authors report the static magnetic structures and dynamic properties of these nanoparticles and the topological defects in 2D and 3D nanoparticles with new examples of S-shaped vortex or antivortex and of bent vortex or antivortex in 3D nanoparticles. The spectrum of magnetic excitations is shown to exhibit the occurrence of gaps, a key for magnonic metamaterial devices. Magnetic excited states are also considered with their coupling to nanoparticle elastic properties.
Download or read book Nucleic Acid Biology and its Application in Human Diseases written by Subhrangsu Chatterjee and published by Springer Nature. This book was released on 2023-05-30 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the structure-function relationship of nucleic acids, their role in the pathophysiology of the diseases, and as therapeutic targets for human diseases. The chapters discuss the role of nucleic acids in inflammatory diseases, neurodegenerative diseases, and cancer. The book also describes recent advancements in nucleic acid-based therapy, the application of nucleic acids in diagnostics, in the development of nano-carriers, logic gates, and sensors. It explores the use of nucleic acids (RNA and DNA) as a unique and multifunctional platform for numerous applications, including therapeutics, diagnostics, nanodevices, and materials. It further examines the role of DNA methylation, histone modifiers and readers, chromatin remodelers, microRNAs, and other components of chromatin in the progression of cancer. The book also discusses the applications of nucleic acid as a vaccine and as a gene-editing tool. It also provides an overview of the clinical trials using genome editing platforms for disease treatment and the challenges in implementing the editing technology. Finally, the book elucidates a representative description of challenges associated with nucleic acid-mediated therapy.
Download or read book 3D DNA Nanostructure written by Yonggang Ke and published by . This book was released on 2016 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This detailed volume presents a comprehensive technical overview of DNA nanotechnology with an emphasis on 3D DNA nanostructure design and applications. Coverage spans from basic design principles for DNA and RNA nanostructures to their cutting-edge applications in a variety of fields, with the book divided into basic DNA and RNA nanostructure design strategies as well as applications utilizing DNA nanostructures, including but not limited to nanomedicine, bioimaging, biosensing, nanoplasmonics, nanoelectronics, nanofabrication, crystallography, biophysics, and analytical chemistry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, 3D DNA Nanostructure: Methods and Protocols provides the most up-to-date tutorial style overviews and technical style protocols to benefit researchers in a wide variety of areas."--OCLC.
Download or read book Visions of DNA Nanotechnology at 40 for the Next 40 written by Nataša Jonoska and published by Springer Nature. This book was released on 2023-07-04 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book provides a unique and state-of-the-art view on DNA nanotechnology with an eye toward future developments. Intended as a tribute to Nadrian C. Seeman, who founded the field of DNA nanotechnology, the content is an exciting mixture of technical and non-technical material, reviews, tutorials, perspectives, new findings, and open questions. The book aims to inspire current researchers to sit back and think about the big picture, while also enticing new researchers to enter the field. Most of all, the book captures voices from a unique moment in time: 40 years after the publication of the first paper that envisioned DNA nanotechnology. From this vantage point, what are the untold stories, the unspoken concerns, the underlying fundamental issues, the overlooked opportunities, and the unifying grand challenges? What will help us see more clearly, see more creatively, or see farther? What is transpiring right now that could pave the way for the future? To address these questions, leading researchers have contributed 22 chapters, grouped into five sections: perspectives, chemistry and physics, structures, biochemical circuits, and spatial systems. This book will be an important reference point in the field of DNA nanotechnology, both for established researchers looking to take stock of the field and its future, and for newcomers such as graduate students and researchers in other fields who are beginning to appreciate the power and applicability of its methods.
Download or read book Encyclopedia of Supramolecular Chemistry Two Volume Set Print written by Jerry L. Atwood and published by CRC Press. This book was released on 2013-10-09 with total page 1745 pages. Available in PDF, EPUB and Kindle. Book excerpt: The two-volume Encyclopedia of Supramolecular Chemistry offers authoritative, centralized information on a rapidly expanding interdisciplinary field. User-friendly and high-quality articles parse the latest supramolecular advancements and methods in the areas of chemistry, biochemistry, biology, environmental and materials science and engineering, physics, computer science, and applied mathematics. Designed for specialists and students alike, the set covers the fundamentals of supramolecular chemistry and sets the standard for relevant future research.
Download or read book Nanobiotechnology written by Christof M. Niemeyer and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanotechnology is the key technology of the 21st century. The possibility to exploit the structures and processes of biomolecules for novel functional materials, biosensors, bioelectronics and medical applications has created the rapidly growing field of nanobiotechnology. Designed as a broad survey of the field, this book combines contributions from bioorganic and bioinorganic chemistry, molecular biology, materials science and bioanalytics to fathom the full scope of current and future developments. It is divided into four main sections: * Interphase Systems * Protein-based Nanostructures * DNA-based Nanostructures * Nanoanalytics Each chapter describes in detail currently available methods and contains numerous references to the primary literature, making this the perfect "field guide" for chemists, biologists and materials scientists who want to explore the fascinating world of nanobiotechnology.
Download or read book 3D Bioprinting and Nanotechnology in Tissue Engineering and Regenerative Medicine written by Lijie Grace Zhang and published by Academic Press. This book was released on 2022-02-18 with total page 563 pages. Available in PDF, EPUB and Kindle. Book excerpt: 3D Bioprinting and Nanotechnology in Tissue Engineering and Regenerative Medicine, Second Edition provides an in-depth introduction to bioprinting and nanotechnology and their industrial applications. Sections cover 4D Printing Smart Multi-responsive Structure, Cells for Bioprinting, 4D Printing Biomaterials, 3D/4D printing functional biomedical devices, 3D Printing for Cardiac and Heart Regeneration, Integrating 3D printing with Ultrasound for Musculoskeletal Regeneration, 3D Printing for Liver Regeneration, 3D Printing for Cancer Studies, 4D Printing Soft Bio-robots, Clinical Translation and Future Directions. The book's team of expert contributors have pooled their expertise in order to provide a summary of the suitability, sustainability and limitations of each technique for each specific application. The increasing availability and decreasing costs of nanotechnologies and 3D printing technologies are driving their use to meet medical needs. This book provides an overview of these technologies and their integration. Includes clinical applications, regulatory hurdles, and a risk-benefit analysis of each technology Assists readers in selecting the best materials and how to identify the right parameters for printing Includes the advantages of integrating 3D printing and nanotechnology in order to improve the safety of nano-scale materials for biomedical applications
