Download or read book Chemical Reagents for Protein Modification Third Edition written by Roger L. Lundblad and published by Springer Science & Business. This book was released on 2005 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Revised and updated, Chemical Reagents for Protein Modification, Third Edition is an encyclopedic work describing the many approaches to the site-specific modification of proteins. More than 2,000 references are cited describing the development of the various reagents. This book explores how the use of site-specific protein modification relates to a wide variety of biotechnology processes and products, such as protein microarrays, hydrogels, controlled-release pharmaceuticals, and biotherapeutics, and proteomics. With chapters focusing on analyses of individual amino acids, additional chapters cover chemical cross-linking and the chemical cleavage of peptide chains. There is considerable reference to the use of site-specific chemical modification in proteomics. The author pays particular attention to the general environmental factors that influence the reactivity of individual amino acid residues. He presents techniques for the characterization of modified proteins, including mass spectrometry, and includes increased coverage of spectroscopic techniques and crystallographic analyses. This is followed by coverage of nitrosylation reactions and other non-enzymatic modification processes. Specific reference is given to the differential labeling of protein functional groups with reagents labeled with stable isotopes. Resources include a list of current journals that publish site-specific chemical modification studies, the most commonly used reagents and their properties, and a contact list of reagent suppliers. Chemical Reagents for Protein Modification, 3rd Edition presents the most frequently used methods for the site-specific chemical modification of proteins, techniques for protein characterization, precise laboratory data for factors that influence reactivity and reproducibility, and industry-specific resources.
Download or read book Chemical reagents for protein modification written by Roger L. Lundblad and published by . This book was released on 2005 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical Reagents for Protein Modification written by Roger L. Lundblad and published by CRC Press. This book was released on 2020-03-11 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1991, Chemical Reagents for Protein Modification, 2nd Edition provides a unique combination of theoretical and practical considerations for the use of chemical reagents for site-specific modification of proteins. The book is divided into three sections, with the first section describing general techniques, including information on the organic chemistry of the various modification reactions; the separation and characterization of site-specific modified proteins, including applications to proteins separated by electrophoresis followed by blotting; the specific chemical cleavage of peptide bonds in proteins; the separation of peptides by high-performance liquid chromatography and electrophoresis; and the use of chemical reagents to assess conformational change in proteins. The second section provides an encyclopedic description of reagents and reactions for the site-specific modification of individual amino acid residues in proteins. The final section presents descriptions of the use of chemical reagents to label biologically significant sites in proteins, including enzyme active sites and the use of covalent cross-linking to measure protein-protein interactions. Particular emphasis is placed on the use of photoaffinity reagents. The book will be an extremely useful research tool for all investigators interested in the solution chemistry of proteins.
Download or read book Chemical Reagents for Protein Modification Fourth Edition written by Roger L. Lundblad and published by CRC Press. This book was released on 2014-07-22 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of the chemical modification of proteins has evolved over the past 80 years, benefiting from advances in analytical, physical, and organic chemistry. Over the past 30 years, the use of chemical reagents to modify proteins has been crucial in determining the function and structure of purified proteins. This groundbreaking work is part of the foundation of emerging disciplines of proteomics, chemical biology, structure biology, and chemical proteomics. Chemical Reagents for Protein Modification, Fourth Edition provides a comprehensive review of reagents used for the chemical modification of proteins, representing a major revision of the work presented in previous editions. The completely updated Fourth Edition is substantially larger and includes five new chapters: Alkylating Agents Acylating Agents Nitration and Nitrosylation Oxidation Modification of Proteins with Reducing Agents There is greatly increased coverage of the chemical modification of cysteine, which is critical for bioconjugate synthesis. The chapter on reduction also provides information necessary for bioconjugate synthesis as well as for the processing of inclusion bodies. The book places emphasis on conditions that affect the specificity of the chemical modification of proteins, such as solvent and temperature. The format has been markedly revised, presenting information based on the chemical nature of the modifying material and on the amino acid residue modified. This new version has increased significance to biopharmaceuticals. Much of the information is in tabular form, which enables the rapid location of cited material.
Download or read book Chemical Reagents for Protein Modification written by Roger L. Lundblad and published by CRC Press. This book was released on 2018-01-18 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: The contents of this book are focused on the use of chemical modification to study the properties of proteins in solution. Particular emphasis has been placed on the practical laboratory aspects of this approach to the study of the relationship between structure and function in the complex class of biological heteropolymers.
Download or read book Chemical reagents for protein modification written by Roger L. Lundblad and published by . This book was released on 1984 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical Reagents for Protein Modification written by Roger L. Lundblad and published by CRC Press. This book was released on 2018-01-18 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: The contents of this book are focused on the use of chemical modification to study the properties of proteins in solution. Particular emphasis has been placed on the practical laboratory aspects of this approach to the study of the relationship between structure and function in the complex class of biological heteropolymers. As a result, little emphasis is given to the individual consideration of the functional consequence of chemical modification.
Download or read book Chemical Reagents for Protein Modification written by Roger L. Lundblad and published by . This book was released on 1989 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Techniques in Protein Modification written by Roger L. Lundblad and published by CRC Press. This book was released on 1994-11-17 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: This exciting laboratory support book encompasses the encyclopedic coverage of the most frequently used methods for the site-specific chemical modification of proteins. In a concise manner, it presents methods for the characterization of modified proteins, including: amino acid analysis protein sequence analysis chemical cleavage of protein chains chromatographic separation of peptides It also discusses various approaches to the determination of solution protein concentration. It includes a complete literature survey of the various reagents, a list of the most commonly used reagents with their physical and chemical properties, and a list of preferred reagent suppliers.
Download or read book CHEMICAL REAGENTS FOR PROTEIN MODIFICATION written by Roger L. Lundblad and published by Springer. This book was released on 1984-04-18 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: The chemical modification of proteins; Amino acid analysis; Peptide separation by reverse-phase high performance liquid chromatography; Methods for sequence determination; Chemical cleavage of peptide bonds; The modification of cystine; The Modification of cystine - cleavage of disulfide bonds; The modification of methionine; Modification of histidine residues; The modification of Lysine.
Download or read book The Proteins Pt 3 written by Hans Neurath and published by Elsevier. This book was released on 2012-12-02 with total page 603 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Proteins, Third Edition, Volume II is a three-chapter text that highlights the application of methods of organic chemistry to the study of protein structure. Chapter 1 reviews the chemical modification of proteins by group- and site-specific reagents. This chapter also discusses the methods for the chemical modification of proteins and their application to the study of the structure, conformation, and biologic activity of certain proteins. Chapter 2 describes the synthesis of peptides by solution methods and the procedures employed for the synthesis of biologically active peptide hormones. This chapter also deals with the many difficulties inherent in the application of the existing synthetic methods and emphasizes the stringent standards that must be maintained for the successful chemical synthesis of naturally occurring polypeptides. Chapter 3 presents the solid-phase methods for the synthesis of peptides on solid supports. Organic chemists and researchers, teachers and undergraduate students will find this book invaluable.
Download or read book Bioconjugate Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 2013-07-25 with total page 1166 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioconjugate Techniques, Third Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates Features full color illustrations Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented
Download or read book Chemistry of Protein and Nucleic Acid Cross Linking and Conjugation Second Edition written by Shan S. Wong and published by CRC Press. This book was released on 2011-10-10 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the first edition of Chemistry of Protein Conjugation and Cross-Linking in 1991, new cross-linking reagents, notably multifunctional cross-linkers, have been developed and synthesized. The completion of the human genome project has opened a new area for studying nucleic acid and protein interactions using nucleic acid cross-linking reagents, and advances have also been made in the area of biosensors and microarray biochips for the detection and analysis of genes, proteins, and carbohydrates. In addition, developments in physical techniques with unprecedented sensitivity and resolution have facilitated the analysis of cross-linked products. Updated to reflect the advances of the 21st century, this book offers: An overview of the chemical principles underlying the processes of cross-linking and conjugation A thorough list of cross-linking reagents published in the literature since the first edition, covering monofunctional, homobifunctional, heterobifunctional, multifunctional, and zero-length cross-linkers Reviews of the use of these reagents in studying protein tertiary structures, geometric arrangements of subunits within complex proteins and nucleic acids, near-neighbor analysis, protein-to-protein or ligand–receptor interactions, and conformational changes of biomolecules Discusses the application of immunoconjugation for immunoassays, immunotoxins for targeted therapy, microarray technology for analysis of various biomolecules, and solid state chemistry for immobilizations
Download or read book The Proteins written by Hans Neurath and published by . This book was released on 1975 with total page 716 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Proteins Pt 3.
Download or read book The Evolution from Protein Chemistry to Proteomics written by Roger L. Lundblad and published by CRC Press. This book was released on 2005-10-14 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Largely driven by major improvements in the analytical capability of mass spectrometry, proteomics is being applied to broader areas of experimental biology, ranging from oncology research to plant biology to environmental health. However, while it has already eclipsed solution protein chemistry as a discipline, it is still essentially an extension
Download or read book Chemical Modification of Proteins written by Gary E. Means and published by . This book was released on 1971 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Anticancer Research written by and published by . This book was released on 2005 with total page 802 pages. Available in PDF, EPUB and Kindle. Book excerpt:
