Download or read book Characterization of the Phytophthora Infestans Secretome and Identification of a Protein that Suppresses Necrosis in Tomato written by Brendan Stallard Kelley and published by . This book was released on 2006 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Functional Characterization of Extracellular Protease Inhibitors of Phytophthora Infestans written by Miaoying Tian and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: The oomycetes form one of several lineages within the eukaryotes that independently evolved a parasitic lifestyle and are thought to have developed unique mechanisms of pathogenicity. The devastating oomycete plant pathogen Phytophthora infestans causes late blight, a ravaging disease of potato and tomato. Little is known about processes associated with P. infestans pathogenesis, particularly the suppression of host defense responses. We used data mining of P. infestans sequence databases to identify 18 extracellular protease inhibitors belonging to two major structural classes: (i) Kazal-like serine protease inhibitors (EPI1 to EPI14) and (ii) cystatin-like cysteine protease inhibitors (EPIC1 to EPIC4). A variety of molecular, biochemical and bioinformatic approaches were employed to functionally characterize these genes and investigate their roles in pathogen virulence. The 14 EPI proteins form a diverse family and appear to have evolved by domain shuffling, gene duplication, and diversifying selection to target a diverse array of serine proteases. Recombinant EPI1 and EPI10 proteins inhibited subtilisin A among major serine proteases, and inhibited and interacted with tomato P69B subtilase, a pathogenesis-related protein belonging to PR7 class. The recombinant cystatin-like cysteine protease inhibitor EPIC2B interacted with a novel tomato papain-like extracellular cysteine protease PIP1 with an implicated role in plant defense. PIP1 is closely related to Rcr3, an apoplastic cysteine protease required for tomato Cf-2 and Cladosporium fulvm Avr2-dependent defense response. Both EPIC1 and EPIC2B interacted with Rcr3. Interactions with plant defense-related proteases suggest a counterdefense role of these extracellular protease inhibitors. Interestingly, EPIC1 and EPIC2B were degraded by tomato pathogenesis-related P69B subtilase and EPI1 protected both proteins from degradation, indicating that EPI1 contributes to virulence by protecting pathogen proteins from degradation by defense-related proteases. In addition, our overall results suggest that complex cascades of inhibition of host proteases by diverse extracellular protease inhibitors of P. infestans might occur in the plant apoplast during infection, thus leading to multifaceted suppression of plant defense responses. Both Kazal-like and cystatin-like inhibitors are widespread in the oomycetes, but have not been reported in other microbial plant pathogens. Inhibition of host proteases by P. infestans protease inhibitors is proposed to be a novel mechanism of pathogen suppression of plant defense.
Download or read book Identification and Characterization of Host Targets of Phytophthora Infestans Rxlr Effectors written by Kamaldeep Kaur Bansal and published by . This book was released on 2014 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: The late blight of potato and tomato caused by Phytophthora infestans is a threat for global food production. The late blight of tomato is an emerging disease in Florida and it causes economic losses worth millions. Phytophthora infestans is an oomycete and it causes infection by haustoria which penetrate inside the host cell and colonize the cells. The pathogen secretes diverse protein molecules known as effectors. The effectors take over the host defense machinery and suppress the immune response. The host target proteins for majority of RxLR effectors are unknown. Two RXLR effectors PITG_16294 and PITG_18221 were used in Yeast Two-Hybrid Screening for identification of possible host targets. PITG_16294 interacted with a number of host proteins but none was identified as true host target. The screening of PITG_18221 revealed that it interacts with CSN5b, which is a subunit of COP9 signalosome and is involved in protein degradation. CSN5b is a disputed host target as it interacts with GAL4-DBD of the yeast in yeast two hybrid assays. However, the truncated CSN5b also showed interaction thus verifying the interaction between CSN5b and PITG_18221. Thus, CSN5b could be a possible host target of RXLR effector PITG_18221.
Download or read book Oomycete Genetics and Genomics written by Kurt Lamour and published by John Wiley & Sons. This book was released on 2009-06-17 with total page 602 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book brings together the knowledge from and tools for genetic and genomic research into oomycetes to help solve the problems this pathogen poses to crops and animals. Armed with the information presented here, researchers can use oomycete data to solve practical problems and gain insight into future areas of interest. Key Features: Offers an up-to-date coverage of research into oomycetes – which has advanced with biochemical and molecular analyses in recent years Helps researchers use oomycete data to solve practical problems, like damage to crop and animal resources Includes a section on interactions with animal hosts Offers perspective on future areas of research Assembles an international author base
Download or read book Genes Involved in Plant Defense written by Thomas Boller and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many fungi and bacteria that associate with plants are potentially harmful and can cause disease, while others enter into mutually beneficial sym bioses. Co-evolution of plants with pathogenic and symbiotic microbes has lead to refined mechanisms of reciprocal recognition, defense and counter defense. Genes in both partners determine and regulate these mechanisms. A detailed understanding of these genes provides basic biological insights as well as a starting point for developing novel methods of crop protection against pathogens. This volume deals with defense-related genes of plants and their regulation as well as with the genes of microbes involved in their interaction with plants. Our discussion begins at the level of populations and addresses the complex interaction of plant and microbial genes in multigenic disease resistance and its significance for crop protection as compared to mono genic resistance (Chap. 1). Although monogenic disease resistance may have its problems in the practice of crop protection, it is appealing to the experimentalist: in the so-called gene-for-gene systems, single genes in the plant and in the pathogen specify the compatibility or incompatibility of an interaction providing an ideal experimental system for studying events at the molecular level (Chaps. 2 and 4). Good progress has been made in identifying viral, bacterial, and fungal genes important in virulence and host range (Chaps. 3-6). An important aspect of plant-microbe interactions is the exchange of chemical signals. Microbes can respond to chemical signals of plant origin.
Download or read book Phytophthora written by Salomon Bartnicki-Garcia and published by . This book was released on 1983 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biology of Phythophthora; Taxonomy of Phytophthora; Ecology of Phytophthora; Pathology of Phytophthora.
Download or read book Endophytes for a Growing World written by Trevor R. Hodkinson and published by Cambridge University Press. This book was released on 2019-03-21 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discusses the role of endophytes in food security, forestry and health. It outlines their general biology, spanning theory to practice.
Download or read book Plant pathogen Interactions written by Nicholas J. Talbot and published by CRC Press. This book was released on 2004 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant diseases are destructive and threaten virtually any crop grown on a commercial scale. They are kept in check by plant breeding strategies that have introgressed disease resistance genes into many important crops, and by the deployment of costly control measures, such as antibiotics and fungicides. However, the capacity for the agents of plant disease - viruses, bacteria, fungi, and oomycetes - to adapt to new conditions, overcoming disease resistance and becoming resistant to pesticides, is very great. For these reasons, understanding the biology of plant diseases is essential for the development of durable control strategies. Plant-Pathogen Interactions provides and overview of our current knowledge of plant-pathogen interactions and the establishment of plant disease, drawing together fundamental new information on plant infection mechanisms and host responses. The role of molecular signals, gene regulation, and the physiology of pathogenic organisms are emphasized, but the role of the prevailing environment in the conditioning of disease is also discussed. Emphasizing the broader understanding that has emerged from the use of molecular genetics and genomics, Plant-Pathogen Interactions highlights those interactions that have been most widely studied and those in which genome information has provided a new level of understanding.
Download or read book Environmental Microbiology Fundamentals and Applications written by Jean-Claude Bertrand and published by Springer. This book was released on 2015-01-26 with total page 933 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a treatise on microbial ecology that covers traditional and cutting-edge issues in the ecology of microbes in the biosphere. It emphasizes on study tools, microbial taxonomy and the fundamentals of microbial activities and interactions within their communities and environment as well as on the related food web dynamics and biogeochemical cycling. The work exceeds the traditional domain of microbial ecology by revisiting the evolution of cellular prokaryotes and eukaryotes and stressing the general principles of ecology. The overview of the topics, authored by more than 80 specialists, is one of the broadest in the field of environmental microbiology. The overview of the topics, authored by more than 80 specialists, is one of the broadest in the field of environmental microbiology.
Download or read book Cacao Diseases written by Bryan A. Bailey and published by Springer. This book was released on 2016-02-22 with total page 630 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the current state of knowledge concerning cacao pathogens and methods for their management. Topics discussed include the history, biology and genetic diversity of Moniliophthora species (which cause witches’ broom and frosty pod rot) and Phytophthora species (which cause black pod rot) that cause diseases resulting in major losses to cacao production. Emerging pathogens such as Cacao swollen shoot virus and Ceratobasidium theobromae (which causes vascular streak dieback) are also discussed in detail, along with many pathogens of significant local concern. Most of these pathogens represent major risks to global cacao production should they expand into new areas, breaking out of their current limited distributions. By considering cacao diseases as a group, similarities in the available tools and techniques used in their management become apparent, as do their limitations. Gaps in our current knowledge of cacao pathogens and the management of the diseases they cause are detailed, and suggestions for future research directions are provided. This insight allows readers to consider cacao disease threats from a more comprehensive, global perspective and paves the way for an improved synergy of efforts between the various research programs, agencies, and industries, both private and public, with vested interests in cacao production, and cacao farmers.
Download or read book Plant Diseases Caused by Dickeya and Pectobacterium Species written by Frédérique Van Gijsegem and published by Springer Nature. This book was released on 2021-01-04 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a detailed review of many different aspects of pathogens, from the effects of single base pair mutations to large-scale control options, bringing into a single volume over 100 years of findings from thousands of researchers worldwide. Diseases caused by soft rot Pectobacteriaceae (SRP) are a major cause of loss to crop, vegetables and ornamental plants worldwide, and have been found on all continents except Antarctica. While different aspects of the SRP have appeared in other books on plant disease, no book, until now, has been dedicated solely to them.
Download or read book Plant Relationships written by Holger B. Deising and published by Springer. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the first edition of "The Mycota Vol. V – Plant Relationships" in 1997, tremendous advances in fungal molecular biology and biochemistry have taken place; and both light and electron microscopical techniques have improved considerably. These new insights led to a better understanding of the relationships between fungi and plants; and a completely revised new edition of Plant Relationships could be produced, providing an up-to-date overview on mutualistic and pathogenic interactions. In 18 chapters internationally acknowledged authors present reviews on fungal lifestyles, mechanisms of their interactions with their host plants, signal perception and transduction, and plant defense responses directed against attack by fungal pathogens. Highlighting the recent developments in fungus-plant interactions, this volume is indispensable for researchers, lecturers and students in microbiology, mycology and plant sciences, including plant pathology.
Download or read book Current Trends in Plant Disease Diagnostics and Management Practices written by Pradeep Kumar and published by Springer. This book was released on 2016-04-28 with total page 471 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant diseases play an important role on our daily lives. Most of plant diseases are visible and are caused by biotic and/or abiotic factors. Symptoms are usually the results of a morphological change, alteration or damage to plant tissue and/or cells due to an interference of the plant’s metabolism. All basic structures of vascular plants are subject to attack by pathogens. The failure in accurate disease diagnosis and management may lead to huge losses in plant production and related commodities, which causes nutritional food scarcity. Typically, the appearance of a biotic symptom will indicate the relatively late stage of an infection and/or colonization of a pathogen. Expert detection, accurate diagnosis, and timely management play a significant role in keeping plants free from pathogens. In this book expert scholars share their research knowledge and key literature which are vital toward the diagnosis of plant diseases across the globe, addressing traditional plant pathology techniques, as well as advanced molecular diagnostic approach.
Download or read book Molecular Aspects of Plant Pathogen Interaction written by Archana Singh and published by Springer. This book was released on 2018-02-15 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book offers an integrated overview of plant–pathogen interactions. It discusses all the steps in the pathway, from the microbe–host-cell interface and the plant’s recognition of the microbe to the plant’s defense response and biochemical alterations to achieve tolerance / resistance. It also sheds light on the classes of pathogens (bacteria, fungus and viruses); effector molecules, such as PAMPs; receptor molecules like PRRs and NBS-LRR proteins; signaling components like MAPKs; regulatory molecules, such as phytohormones and miRNA; transcription factors, such as WRKY; defense-related proteins such as PR-proteins; and defensive metabolites like secondary metabolites. In addition, it examines the role of post-genomics, high-throughput technology (transcriptomics and proteomics) in studying pathogen outbreaks causing crop losses in a number of plants. Providing a comprehensive picture of plant-pathogen interaction, the updated information included in this book is valuable for all those involved in crop improvement.
Download or read book Bacterial Wilt Disease written by Philippe Prior and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: Jointly published with INRA, Paris. Bacterial wilt, caused by Ralstonia solanacearum, is a very destructive plant disease that attacks over 450 different species, including many of the most important economic crop plants. Often endemic, the bacterium transmits through the soil, penetrates the plant root system and eventually causes irreversible wilting and death. This book summarizes the current information on bacterial wilt for both the basic research community and for concerned professionals who are faced with the disease in the field, offering the latest approaches to diagnosis and control of the disease. Emphasis is placed on integrated and biologically sustainable control methods. Also presented is the most recent genetic/biochemical research exploring the interaction between the bacterium and its plant host at the molecular level.
Download or read book Chemistry Biochemistry and Biology of 1 3 Beta Glucans and Related Polysaccharides written by Antony Bacic and published by Academic Press. This book was released on 2009-07-07 with total page 713 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemistry, Biochemistry, and Biology of 1-3 Beta Glucans and Related Polysaccharides presents a comprehensive, systematic and authoritative survey of information about a family of chemically related, but functionally diverse, naturally occurring polysaccharides--the (1-3)-glucans. International contributors describe the chemical and physicochemical properties of these glucans and their derivatives and the molecular biological and structural aspects of the enzymes involved in their formation and breakdown. A detailed analysis of their physiological roles in the various biological situations in which they are found will be provided. Additionally, evolutionary relationships among the family of these glucans will be described. Topics of medical relevance include detailing the glucans' interactions with the immune system and research for cancer therapy applications Web resource links allow scientists to explore additional beta glucan research Separate indexes divided into Species and Subject for enhanced searchability
Download or read book Natural Antimicrobial Agents written by Jean-Michel Mérillon and published by Springer. This book was released on 2018-02-08 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Documenting the latest research in the field of different pathogenic organisms, this book presents the current scenario about promising antimicrobials in the following areas: Part I. Plants as source of antibacterials, Part II. Naturally occurring antifungal natural products, Part III. Antiparasitic natural products, Part IV. Antiviral natural products. Renowned scientists from the globe have been selected as authors to contribute chapters. Use of plants for various ailments is as old as human civilization and continuous efforts are being made to improve medicinal plants or to product their bioactive secondary metabolites in high amounts through various technologies. About 200,000 natural products of plant origin are known and many more are being identified from higher plants and micro-organisms. Some plants based drugs are used since centuries and there is no alternative medicine for many such drugs as cardiac glycosides. Drug discovery from medicinal plants or marine micro-organisms continues to provide an important source of new drug leads. Research on new antibacterials represents a real and timely challenge of this century, particularly for the treatment of infections caused by clinical isolates that show multidrug resistance. The main microorganisms involved in the resistance process have been identified and given the acronym ESKAPE for Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa and Enterobacteriaceae. Multidrug resistant Mycobacterium tuberculosis including highly drug-resistant strains (XDR-TB) has also emerged as one of the most important clinical challenges of this century. Plants of diverse taxa and marine micro-organisms are rich source of these antimicrobials. An attempt has been made to compile the recent information about natural sources of antibacterials and their sustainable utilization. Increased panic of these pathogens warrants a growing demand for research to undertake the threat of multidrug resistance. The search for new antifungal, antiparasitic and antiviral natural products is far from devoid of interest. According to the WHO report in 2013, malaria still represents some 207 million cases worldwide and more than 3 billion of people are still exposed to this risk. Similarly, about 350 million people are considered at risk of contracting leishmaniasis. The fight against some viruses also requires that the research on natural products continue. For example, even if an antiretroviral with direct action was recently approved in Europe in 2013, its high cost does not allow to offer it to an exposed population in countries where the cost of drugs remains a problem for a large part of the population. These books are useful to researchers and students in microbiology, biotechnology, pharmacology, chemistry and biology as well as medical professionals.
