Download or read book Characterization of Immortalized and Malignant Adult Human Prostate Epithelial Cell Lines written by Diana Bello-DeOcampo and published by . This book was released on 1996 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Contributions of 8P Loss and 8Q Gain to the Malignant Phenotype in Human Prostate Tumors written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prostate cancer research is hampered by the lack of suitable human in vitro models that accurately recapitulate the genetic composition or biological behavior of initiate primary prostate tumors. In order to overcome this limitation, the Nl5C6 epithelial and the Nl fibroblastic cell lines were developed through immortalization of explanted human prostate tissue with the HPV and E6 and E7 proteins. Genotypic characterization revealed that immortalization of the Nl5C6 epithelial cells was associated with a dominant genetic alteration involving the short arm of chromosome 8, a region often deleted or rearranged in primary human prostate tumors. Phenotypic characterization demonstrated that the Nl5C6 epithelial and Nl fibroblastic cell lines expressed cell-type appropriate proteins and gene transcripts. The Nl5C6 cells, but not Nl cells, expressed anchorage independence, consistent with an initially transformed phenotype. Moreover, Nl5C6 epithelial cells proliferated and formed colonies in soft agar at rates 2-3 fold higher when grown in Nl fibroblast conditioned serum free media compared to unconditioned serum free media. These results suggest that factors secreted by Nl fibroblasts augmented expression of the malignant phenotype by Nl5C6 epithelial cells, and show that paracrine interactions defined the malignant potential of transformed epithelial cells in the human prostate.
Download or read book Molecular and Cellular Biology of Prostate Cancer written by James P. Karr and published by Springer Science & Business Media. This book was released on 1991 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: I. Intracellular Communications.- Tissue Specificity and Cell Death are Associated with Specific Alterations in Nuclear Matrix Proteins.- Mechanism of Growth Regulation in Androgen Responsive Cells.- The Impact of Androgen, Extracellular Matrix, and Stroma upon Proliferation and Differentiation of Benign and Malignant Prostate Epithelial Cells.- Therapeutic Approaches to Activating Programmed Cell Death of Androgen-Independent Prostatic Cancer Cells.- Cell Motility and Structural Harmonics in Prostate Cancer.- Panel Discussion.- II. Growth Factors - 1.- Studies of the Endocrine and Paracrine Effect of Tumor Produced Factors in Human Genitourinary Cancers.- Fibroblast Growth Factor: Implications in the Etiology of Benign Prostatic Hyperplasia.- Fibroblast-Mediated Human Epithelial Tumor Growth and Hormonal Responsiveness In Vivo.- Polyamine Requirement of Prostate Cancer Cell Proliferation.- Heparin-Binding (Fibroblast) Growth Factor/Receptor Gene Expression in the Prostate.- Characterization and Partial Purification of a Non - Heparin-Binding Prostate Growth Factor From Cancerous Human Prostate.- Panel Discussion.- Growth Factors - 2.- Transforming Growth Factor a: A Potential Autocrine Growth Regulator in Prostatic Carcinoma.- Prostatic Growth Factors (PrGFs) - From the Identification of Probasin to the Role of PrGFs.- Urogenital Sinus Derived Growth Inhibitory Factor.- Growth Factor Antagonists in Prostate Cancer: Suramin and Cytotoxic Polyamines as Potential Therapy.- Transforming Growth Factors in Human Prostate Cancer.- Gene Products as the Motivating Force in the Prostate Cell's Response to Androgens.- Panel Discussion.- III. Steroid Receptors.- Molecular Biology of Prostate - Specific Antigen.- Structure and Expression of the Androgen Receptor in Normal Tissues and in Prostate Carcinoma Cell Lines.- Structural Analysis and Gene Expression of TR2 Receptor and TR3 Receptor.- cDNA Cloning, Antibody Production and Immunohistochemical Localization of the Androgen Receptor.- New Approaches to Studies on the Androgen Receptor.- Specific Receptors for Vitamin D3 in Human Prostatic Carcinoma Cells.- Panel Discussion.- IV. Poster Presentations.- Role of Androgens and Extracellular Matrix in the Growth and Differentiation of Benign and Malignant Prostatic Epithelial Cells.- Tissue Specificity and Cell Death Are Associated with Specific Alterations in Nuclear Matrix Proteins.- ElTect of Transformation on Rat Prostatic Fibroblasts: Alterations In Extracellular Matrix and Cytoskeleton Gene Expression with Retention of Androgen Responsiveness and Androgen Receptor Expression.- A Potential Role for the MDR-1 Gene in the Development of Androgen-Independent Tumors.- Relevance of Low Androgen Levels and Adrenal Androgens in the Growth of Transplantable Human Prostatic Carcinomas.- Growth-Stimulating Effect of Growth Factor(s) from Androgen Independent Tumor Cells (CS 2-Cell) on Androgen Responsive Tumor Cells.- The Cellular Form of Human Prostatic Acid Phosphatase May Function as a Phosphotyrosyl Protein Phosphatase in Cells.- Expression of Prostate Antigen in LNCaP Cells in Culture.- Allelic Expression of the Mouse Ren-1 Genes in the Anterior Prostate (Coagulating Gland).- V. Dna Structure and Gene Expression.- Genomic Alterations in Prostatic Cancer.- Regulation of Gene Expression in the Prostate.- Androgen Regulation of HBGF I-(aFGF) and Characterization of the Androgen-Receptor mRNA in the Human Prostate Carcinoma Ceil Une - LNCaP/A-dep.- DNA Methylation, Differentiation and Cancer.- Evidence for tbe Involement of Genetic Differences and Mesenchymal Factors in the Progression of Oncogene - Induced Prostate Cancer in Reconstituted Mouse Prostate.- Differential Hybridization Analysis as a Tool to Study Prostatic Cancer Metastasis.- Molecular Biology of Androgen Acceptors in Prostatic Cancer Cells.- Panel Discussion.- Panel Discussion.- Panel Discussion.- Panel Discussion.- Panel Discussion.- Contributors.
Download or read book Identification of Tumor associated Proteins in Human Prostatic Epithelial Cell Lines Squamous Cell Carcinoma of Head and Neck by Proteomic Technology written by and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: (Uncorrected OCR) Abstract of thesis entitled Identification of Tumor-Associated Proteins in Human Prostatic Epithelial Cell Lines & Squamous Cell Carcinoma of Head and Neck by Proteomic Technology Submitted by CHEN JIA for the degree of Master of Philosophy at The University of Hong Kong in October 2004 Proteomics defines a research field aiming to characterize molecular and cellular dynamics in protein expression and function on a global level. It has been a powerful tool widely used in recent years in protein expression mapping by two-dimentioanal electrophoresis and mass spectrometry. Prostate cancer is the most commonly diagnosed cancer in Western men. The incidence and mortality rates of prostate cancer in Asian countries, such as China and Japan, appear to increase much in the last two decades. We use proteomics to examine four HPE cell models: 267B1, Ki-ros/267Bl, Ki-ros/267Bl/m and XR/267B1, representing immortalized and ras/XR transformed/tumorigenic HPE cells. Not too many proteins were identified as differently expressed in the transformed/tumorigenic HPE cell lines and the immortalized HPE cells except for the fragments of 7 proteins including GRP78 (up-regulated in ras transforrned/tumorigenic HPE cell lines only) and HSP70 (up-regulated in both ras transforrned/tumorigenic and XR transformed HPE cell line) from HSP70 family, nuclear splicing related hnRNPs (hnRNP Al and A2) and snRNP (SF3a60) and energy metabolism related DLST and ATP synthase P chain (all these 5 were up-regualted in both ras and XR transformed not in ras tumorigenic HPE cell lines). Further investigation is needed to characterize the proteome changes in these HPE cell models to fully understand their relationship, the impact of ras oncogene transformation and the X-ray irradiation transformation, and also the tumorigenic process occurring on the tissue cultured re-established HPE tumor cells. Squamous cell carcinoma (SCC) of the head and neck is the fifth most common cancer worldwid.
Download or read book Molecular Biology of Prostate Cancer written by Manfred Wirth and published by Walter de Gruyter. This book was released on 2013-05-22 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Prostate Cancer Methods and Protocols written by Pamela J. Russell and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prostate cancer is the second leading cancer in men in Western society. A major concern, and an area of intensive research, involves understanding why certain prostate cancers remain localized or indolent, whereas others become aggressive and metastasize. The differences between these cancer types have profound implications for patients and physicians. Indolent d- ease, which grows very slowly, generally does not cause any problems to the patient, whereas aggressive disease requires immediate treatment, the earlier the better. At present, there are no markers that discriminate between these two entities, thus causing a dilemma for the management of patients who have recently been diagnosed. The aim of Prostate Cancer Methods and Protocols is to explore cutting-edge molecular methods that may have the potential to reveal markers of disease for use in more accurate diagnoses of prostate c- cer and, consequently, to lead to new treatment strategies. This book provides a comprehensive collection of both in vitro and in vivo step-by-step protocols currently used by leaders in prostate cancer research, advice on approaches that can be used in the study of prostate cancer, as well as reviews covering areas less amenable to laboratory research, such as environmental factors in prostate cancer, to provide the reader with an overview of the prostate cancer research field as it currently stands.
Download or read book International Journal of Oncology written by and published by . This book was released on 2000 with total page 660 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book International Review of Cytology written by and published by Elsevier. This book was released on 2000-06-22 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: International Review of Cytology presents current advances and comprehensive reviews in cell biology-both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.Key Features* Cellular and Molecular Biology of Capacitation and Acrosome Reaction in Spermatozoa* Growth Factors and Epithelial-Stromal Interactions in Prostate Cancer Development* PCR-Detected Genome Polymorphism in Malignant Cell Growth* Cellulose Microfibrils in Plants: Biosynthesis, Deposition, and Integration into the Cell Wall* Cell Biology of Peroxisomes and Their Characteristics in Aquatic Organisms* Cellular Responses to Vasectomy
Download or read book The Molecular Basis of Human Cancer written by William B. Coleman and published by Humana Press. This book was released on 2016-11-11 with total page 868 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the concepts of molecular medicine and personalized medicine. Subsequent chapters cover the topics of genomics, transcriptomics, epigenomics, and proteomics, as the tools of molecular pathology and foundations of molecular medicine. These chapters are followed by a series of chapters that provide overviews of molecular medicine as applied broadly to neoplastic, genetic, and infectious diseases, as well as a chapter on molecular diagnostics. The volume concludes with a chapter that delves into the promise of molecular medicine in the personalized treatment of patients with complex diseases, along with a discussion of the challenges and obstacles to personalized patient care. The Molecular Basis of Human Cancer, Second Edition, is a valuable resource for oncologists, researchers, and all medical professionals who work with cancer.
Download or read book Cancer Cell Culture written by Simon P. Langdon and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Methods to modify cancer cells are described in Part V, including protocols for transfection, development of drug-resistance, immortalization, and transfer in vivo. In Part VI methods of coculture of different cell types and contamination of cell lines are covered.
Download or read book Xenotransplantation of Human Prostate Cell Lines written by Amanda Sue Rivette and published by . This book was released on 2005 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book International Review of Cytology written by Kwang W. Jeon and published by Elsevier. This book was released on 2003-10-02 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: The acclaimed International Review of Cytology series presents current advances and reviews in cell biology, both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research. Contributors to this volume areViatcheslav M. Mikhailov, Y.C. Wong, XH Wang, PMT Ling, Armin Hallmann, and Carlos G. Dotti.The acclaimed International Review of Cytology series presents current advances and reviews in cell biology, both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.
Download or read book Advanced Therapy of Prostate Disease written by Martin I. Resnick and published by PMPH-USA. This book was released on 2000 with total page 692 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Therapy of Prostate Disease, from the inital to post-surgical psychological concerns, this book is a complete guide to every step of prostate disease treatment. First, it describes the physical exam in detail, as well as laboratory and imaging techniques that can confirm a diagnosis. Then, the pros and cons of treatment methods for every type and variation of prostate cancer and benign conditions are discussed. Post-surgical treatment (including behavioral issues) is also outlined.
Download or read book Anticancer Research written by and published by . This book was released on 1999 with total page 1012 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Culture of Epithelial Cells written by R. Ian Freshney and published by John Wiley & Sons. This book was released on 2004-03-24 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: "...a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years." --Pharmaceutical Research This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.
Download or read book Some Drinking water Disinfectants and Contaminants Including Arsenic written by IARC Working Group on the Evaluation of Carcinogenic Risks to Humans and published by IARC. This book was released on 2004 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: A working group of 23 experts from 13 countries met in Lyon to evaluate the evidence for carcinogenicity of arsenic (mostly naturally occurring) as a contaminant of drinking-water, and of the water-disinfectant chloramine. The working group also evaluated or re-evaluated four chlorination by-products found in drinking-water, namely chloral hydrate, di- and trichloroacetic acids, and 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (also known as MX). High-level exposure to arsenic in drinking-water occurs in some regions such as China, Latin America, Bangladesh and West Bengal. The Working Group reviewed epidemiological studies of human cancer (mainly ecological studies in Taiwan and Chile, and several case-control and cohort studies) in relation to arsenic in drinking-water. Arsenic in drinking-water (primarily inorganic, as arsenate and to a lesser extent arsenite) was evaluated as carcinogenic to humans (Group 1) on the basis of sufficient evidence for an increased risk for cancer of the urinary bladder, lung and skin. Studies on inorganic arsenic in experimental animals provided limited evidence for its carcinogenicity, but sufficient evidence was found in experimental animals for the carcinogenicity of dimethylarsinic acid (an organic form of arsenic), which produced urinary bladder tumours in rats and lung tumours in mice after oral administration.
Download or read book Cancer Cell Lines Part 1 written by John Masters and published by Springer Science & Business Media. This book was released on 2006-04-11 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: Continuous cell lines derived from human cancers are the most widely used resource in laboratory-based cancer research. The first 3 volumes of this series on Human Cell Culture are devoted to these cancer cell lines. The chapters in these first 3 volumes have a common aim. Their purpose is to address 3 questions of fundamental importance to the relevance of human cancer cell lines as model systems of each type of cancer: 1. Do the cell lines available accurately represent the clinical presentation? 2. Do the cell lines accurately represent the histopathology of the original tumors? 3. Do the cell lines accurately represent the molecular genetics of this type of cancer? The cancer cell lines available are derived, in most cases, from the more aggressive and advanced cancers. There are few cell lines derived from low grade organ-confined cancers. This gap can be filled with conditionally immortalized human cancer cell lines. We do not know why the success rate for establishing cell lines is so low for some types of cancer and so high for others. The histopathology of the tumor of origin and the extent to which the derived cell line retains the differentiated features of that tumor are critical. The concept that a single cell line derived from a tumor at a particular site is representative of tumors at that site is naïve and misleading.
