Download or read book Characterization and Expression of Rainbow Trout Oncorhynchus Mykiss Growth Hormone Family Genes written by Bih-Ying Yang and published by . This book was released on 1997 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization and Expression of Rainbow Trout Oncorhynchus Mykiss Growth Hormone Family Genes written by Bih-Ying Yang and published by . This book was released on 1997 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Genetic and Gene Expression Analysis of Growth in Rainbow Trout Oncorhynchus Mykiss written by Brendan F. Wringe and published by . This book was released on 2009 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Rainbow Trout Oncorhynchus Mykiss and Fathead Minnow Pimephales Promelas Cell Lines as Models to Study Pulp and Paper Effluent Effects in Fish written by Caroline Sue Tracy Cheng and published by . This book was released on 2010 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The effects of aquatic toxicants on fish growth and development have been well-documented, in contrast to the changes in gene expression that necessarily precede them. Many of these toxicants are exogenous compounds that possess the ability to mimic steroid hormones or inhibit normal endocrine functions, which are known as endocrine disrupting compounds (EDCs). The Law laboratory previously obtained mRNA expression data indicating that exposure to aquatic effluents from a combined news/kraft pulp and paper mill changed the expression of genes associated with EDC exposure in the livers of fathead minnows (Pimephales promelas). Societal desire to reduce the use of animals in toxicity testing has encouraged the development and use of in vitro systems, including vertebrate cell models. In this study, the gene expression of the rainbow trout (Oncorhynchus mykiss) liver cell line RTL-W1 and the fathead minnow liver cell line FHM-L was examined following effluent exposure. In the RTL-W1 and FHM-L cell lines, 24 h exposure to effluents was sufficient for assessing changes in gene expression, with maximum changes observed between 4 and 6 h after exposure.

Download or read book Expression Vectors for Gene Transfer in Fish written by Yunhan Hong and published by . This book was released on 1993 with total page 131 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Developmental Expression and Evolution of Growth Hormone releasing Hormone and Pituitary Adenylate Cyclase activating Polypeptide in Teleost Fishes Rainbow Trout Oncorhynchus Mykiss and Zebrafish Danio Rerio written by Sandra Lea Krueckl and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Growth hormone-releasing hormone (GRF) and pituitary adenylate cyclase-activating polypeptide (PACAP) are members of the PACAP/Glucagon superfamily. The family is proposed to have developed from an ancestral PACAP-like molecule in invertebrates. Through successive exon, gene and genome duplications the family has grown to include seven other members. In mammals GRF and PACAP are located on different genes, but in fish, amphibians and birds they are located on the same gene. The main function of GRF is the release of growth hormone (GH) from the pituitary. Also, during development GRF influences the fetal pituitary and stimulates GH release during late gestation. In contrast, the functions of PACAP are extremely varied. PACAP is the newest member of the superfamily and there is still much work to be done before its actions are well understood. Like GRF, PACAP is a releasing hormone acting on the pituitary and in addition, the adrenal gland, pancreas and heart, as well as other organs. Also, PACAP regulates smooth muscle in the vascular system, gut, respiratory tract and reproductive tract During development PACAP affects proliferation, differentiation and apoptosis. GRF and PACAP are expressed throughout development in fish, beginning during the blastula period in rainbow trout and at the end of gastrulation in zebrafish (earliest stage examined). In rainbow trout the grf/pacap gene is expressed as two transcripts, a short and a long transcript. The short transcript is produced by alternative splicing of the gene and does not include the fourth exon which codes for GRF. The long transcript includes the coding regions for both GRF and PACAP. By this means PACAP can be regulated separately from GRF. With the extensive role PACAP appears to play in development, separate regulation of the hormone may be necessary. Expression of the grf/pacap gene in zebrafish is widespread early in development and gradually becomes localized. Of particular interest is the expression of the grf/pacap transcript in regions associated with the prechordal plate, an important organizing center in development. Although it is not yet confirmed, there is evidence to suggest GRF and PACAP are expressed in the prechordal plate and its derivatives in the gut and hatching gland. In addition, expression of the grf/pacap transcript is observed in the neuroectoderm (eye, brain and spinal cord) and the developing heart. Considering the expression pattern of GRF and PACAP, I propose that one of both of these hormones may be involved in patterning during vertebrate embryogenesis. The evolution of gene families is thought to occur through successive exon, gene and genome duplications. Duplicate exons or genes become differentiated and eventually gain new functions or become functionless. During evolution of the grf/pacap lineage, several duplication events have occurred. Analysis of rainbow trout leads me to think that this fish and other salmonids possess two copies of the grf/pacap gene. This is not unexpected considering the tetraploid nature of salmonids. Present day mammals encode GRF and PACAP on separate genes. At some point during the evolution of this lineage a duplication event has occurred, possibly in early mammals or prior to the divergence of birds. The study of multigene families is a useful way to understand evolutionary processes. To this end I examined three members of multigene families from sockeye salmon. Therefore, in addition to the evolutionary mechanisms and pathways that directed grf/pacap gene evolution, I examined the ferritin-H subunit, the alpha-tubulin subunit and the beta-globin subunit. These cDNA sequences are similar to their counterparts in other teleost. The evolution of the ferritin gene family is particularly interesting because it involves the addition or deletion of DNA sequences that affect regulation and cytosolic location.

Download or read book Characterization of a Cypia Gene Expression in Rainbow Trout written by Julie Ann Hong and published by . This book was released on 1999 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Characterization of the beta adrenoceptor Gene family of Rainbow Trout Oncorhynchus Mykiss written by James G. Nickerson and published by . This book was released on 2003 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Differential Expression of Growth Genes in the Liver and White Muscle of Small and Large Rainbow Trout Oncorhynchus Mykiss Produced in Two Seasons and Co localization with Quantitative Trait Loci written by Andrea Kocmarek and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Functional Characterization of Rainbow Trout Oncorhynchus Mykiss Chemokine 2 CK 2 electronic Resource written by Shathi Eshaque and published by University of Waterloo. This book was released on 2006 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cumulated Index Medicus written by and published by . This book was released on 2000 with total page 1828 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Characterization of the MX Genes of Rainbow Trout Oncorhynchus Mykiss written by Grant David Trobridge and published by . This book was released on 1996 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Hybridization Between Yellowstone Cutthroat Trout and Rainbow Trout Alters the Expression of Muscle Growth Related Genes and Their Relationships with Growth Patterns written by Carl O. Ostberg and published by . This book was released on 2015 with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hybridization creates novel gene combinations that may generate important evolutionary novelty, but may also reduce existing adaptation by interrupting inherent biological processes, such as genotype-environment interactions. Hybridization often causes substantial change in patterns of gene expression, which, in turn, may cause phenotypic change. Rainbow trout (Oncorhynchus mykiss) and cutthroat trout (O. clarkii) produce viable hybrids in the wild, and introgressive hybridization with introduced rainbow trout is a major conservation concern for native cutthroat trout. The two species differ in body shape, which is likely an evolutionary adaptation to their native environments, and their hybrids tend to show intermediate morphology. The characterization of gene expression patterns may provide insights on the genetic basis of hybrid and parental morphologies, as well as on the ecological performance of hybrids in the wild. Here, we evaluated the expression of eight growth-related genes (MSTN-1a, MSTN-1b, MyoD1a, MyoD1b, MRF-4, IGF-1, IGF-2, and CAST-L) and the relationship of these genes with growth traits (length, weight, and condition factor) in six line crosses: both parental species, both reciprocal F1 hybrids, and both first-generation backcrosses (F1 x rainbow trout and F1 x cutthroat trout). Four of these genes were differentially expressed among rainbow, cutthroat, and their hybrids. Transcript abundance was significantly correlated with growth traits across the parent species, but not across hybrids. Our findings suggest that rainbow and cutthroat trout exhibit differences in muscle growth regulation, that transcriptional networks may be modified by hybridization, and that hybridization disrupts intrinsic relationships between gene expression and growth patterns that may be functionally important for phenotypic.

Download or read book International Review of Cytology written by and published by Academic Press. This book was released on 1998-08-12 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: International Review of Cytology presents current advances and comprehensive reviews in cell biology-both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research. Phylogeny of the Insulin-Like Growth Factors Secretion of Mucous Granules and Other Membrane-Bound Structures Immune-Endocrine Interactions in the Mammalian Adrenal Gland Ryanodine-Sensitive Ca2+-Release Channels

Download or read book Analysis of Immune Gene Expression in Infected and Vaccinated Rainbow Trout Oncorhynchus Mykiss with a Focus on Cytokines of Adaptive Immunity written by and published by . This book was released on 2012 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Neuroendocrine immune Interactions written by Rolf C. Gaillard and published by Karger Medical and Scientific Publishers. This book was released on 2002 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: Interactions between the immune, endocrine and nervous systems seldom appear as main issues in the neurosciences and in immunology. So far this was most likely due to the need to focus on the molecular and cellular bases of single neural, endocrine and immune processes. But hormones, neurotransmitters and neuropeptides can also influence more subtle mechanisms underlying immune cell activity. The contents of this volume aim at listing some aspects which show that not only the bases for neuroendocrine control of more refined mechanisms related to the organization and functioning of the immune systems to exist, but also that the immune system can actively communicate with neuroendocrine structures. The evidence is divided into three categories: - Anatomical, cellular and molecular bases for the exchange of information between immune, endocrine and neural cells, - reciprocal effects between immune and neuroendocrine mechanisms, and - immune-neuroendocrine regulatory circuits. Immunologically triggered neuroendocrine responses can be either beneficial or deleterious for the host. A systematic approach would imply the simultaneous evaluation of neuroendocrine and immune parameters and thus provide the basis for therapeutic interventions based on antagonizing or blocking undesirable effects.

Download or read book Characterization of a Ras Gene in Rainbow Trout written by Kathy A. Mangold and published by . This book was released on 1989 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rainbow trout (Oncorhynchus mykiss) model of chemical carcinogenesis is becoming increasingly important as a supplement to rodent studies. However, much of the molecular biology of the carcinogenic response is still unknown in the trout model. The ras gene family has been implicated in the tumorigenesis of both spontaneous and chemically-induced tumors in mammals. This study is the first to characterize a ras proto-oncogene in rainbow trout. To accomplish this, the ras gene sequence was amplified in vitro by using polymerase chain reaction (PCR). Two synthetic and degenerative oligonucleotide sequences based on a consensus mammal/goldfish ras sequence were used as primers in the PCR procedure. An 800 base pair (bp) sequence was amplified from trout genomic DNA and hybridized with a human c-Ha-ras sequence. The initial amplifications of trout liver cDNA using the PCR procedure with the synthetic ras primers resulted in a single product of approximately 216 bps. However, this amplified "trout" 216 by product was subsequently shown to be an artifact of carryover from a human Ki-ras plasmid. Carryover is a common problem found in many laboratories involved with the PCR procedure, and extensive precautions were used to eliminate the problem in our laboratories. The 800 by PCR product was cloned and sequenced using Taq polymerase. RT-8, a clone containing the 800 bp insert, was shown to have 91% homology to the first two exons of mammalian c-Ha-ras gene and lesser homology to other ras genes. Amplification of trout liver cDNA using specific primers based on the RT-8 sequence resulted in the amplification of sequences identical to the sequence of the RT-8 insert without an intron, as well as unique sequences, which may represent additional trout ras genes. The PCR procedure was modified to identify sequence information immediately 3' of the known trout ras sequence. Partial sequences of at least two different trout ras genes are presented. With this new information, analysis of DNA sequence information from chemically initiated tumors may elucidate the role activation of ras genes plays in the trout model of carcinogenesis.