Download or read book Centrifugal Separation of Plant Cells written by Robert Lighthart and published by . This book was released on 1955 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Centrifugal Separations in Molecular and Cell Biology written by G.D. Birnie and published by Butterworth-Heinemann. This book was released on 2014-06-28 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Centrifugal Separations in Molecular and Cell Biology focuses on the application of modern centrifugation technology in molecular and cell biology, including the separation and fractionation of biological particles by centrifugation on the preparative and analytical scales. The selection first covers the principles and practices of centrifugation and the bases of centrifugal separations. Discussions focus on the basic concepts of sedimentation theory, centrifugation methods, designing centrifugation experiments, care of centrifuges and rotors, and statistical estimation of molecular parameters. The book also ponders on the practical aspects of rate-zonal centrifugation, including gradient materials, density and viscosity of glycerol solutions, and resolution and gradient shape. The publication examines fractionations in zonal rotors and the quantitative aspects of rate-zonal centrifugation. The text then reviews isopycnic centrifugation in ionic media and analytical centrifugation. Topics include separation by isopycnic banding, large-scale preparative procedures, and density-gradient solutes. The selection is a valuable reference for readers interested in centrifugation technology.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation of Membranes and Organelles from Plant Cells written by John Lloyd Hall and published by . This book was released on 1983 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: General principles of cell fractionation. Cell walls. Plasma membranes. Higher plant vacuoles and tonoplasts. Endoplasmic reticulum and ribosomes. The golgi apparatus. Mitochondria and sub-mitochondrial particles. Chloroplasts. Microbodies. Nuclei. Partition in aqueous polymer two-phase systems - a rapid method for separation of membranes particles according to their surface properties.
Download or read book Separating Cells written by Dipak Patel and published by Garland Science. This book was released on 2000-06-15 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Separating Cells: The basics provides user-friendly and practical guidance to the techniques most commonly used to separate cells. The book offers a concise overview of the fundamental principles and explains the 'what, how and why'. This title will be of considerable interest to newcomers to these techniques.
Download or read book Cell Separation written by Derek Fisher and published by OUP Oxford. This book was released on 1998-11-26 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques for separating cells are needed in many areas of cell biology. This book presents modern methods from the laboratories of experts in the field, and includes tested, reproducible protocols, hints and tips for success, and troubleshooting suggestions. It will be invaluable to a wide range of cell biologists.
Download or read book Separation of Plant Cells Using Ficoll Gradients written by Johanna Galindo and published by . This book was released on 2019 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants are an important source for different pharmaceutical molecules. This often results in the destruction of certain plants. Plant cell culture is a technique that allows the production of molecules from a small explant rather than the destruction of naturally occurring habitats and ecosystems. Plan callus cell populations have the potential for viable production of secondary metabolites if we are able to select highly productive cell lines which produce the specific molecule of interest. Fractionation by density and sixe of the individual cell populations through Ficoll density gradient centrifugation and separation can be the solution for maintaining cell lines that produce a specific secondary metabolite. ... This study compares diffferent Ficoll density gradients to successfully separate plant cell lines or plant cell aggregates from four different types of plants including Beta vulgarius, Daucus carota, Nicotiana tabacum, and Taxus spp. These plant cell suspension cultures could potentially be used to produce more secondary metabolites.
Download or read book Centrifugation written by David Rickwood and published by . This book was released on 1984 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edition provides extensive experimental details of protocols for all types of centrifugal separations from macrmolecules to whole cells. It also describes the applications of centrifuges ranging from simle bench machines to analytical centrifuges.
Download or read book The Effect of Centrifugal Force and Temperature on Plant Cells written by Henry Northen and published by . This book was released on 1934 with total page 46 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Centrifugal Separations in Biotechnology written by Wallace Woon-Fong Leung and published by Butterworth-Heinemann. This book was released on 2020-03-13 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: Centrifugal Separations in Biotechnology, Second Edition, is the only book on the market devoted to centrifugal separation in biotechnology. Key topics covered include a full introduction to centrifugation, sedimentation and separation; detailed coverage of centrifuge types, including batch and semi-batch centrifuges, disk-stack and tubular decanter centrifuges; methods for increasing solids concentration; laboratory and pilot testing of centrifuges; selection and sizing centrifuges; scale-up of equipment, performance prediction and analysis of test results using numerical simulation. Centrifugal Separations in Biotechnology, Second Edition, provides guidance on troubleshooting and optimizing centrifuges, and then goes on to explore the commercial applications of centrifuges in biotechnology. It gives detailed process information and data to assist in the development of particular processes from existing systems. It is of value to professionals in the chemical, bioprocess, and biotech sectors, and all those concerned with bioseparation, bioprocessing, unit-operations and process engineering. - Provides a comprehensive guide to centrifuges, their optimal development, and their operation in the biotechnology industry - Updated throughout based on developments in industrial applications and advances in our understanding of centrifugal separations in biotechnology - Discusses applications for the separation of proteins, DNA, mitochondria, ribosomes, lysosomes and other cellular elements - Includes new sections on use of optimal polymer dosage in waste treatment, new centrifuge designs for applications in algae processing, biopharma, and more
Download or read book Cell Separation in Plants written by Daphne J. Osborne and published by Springer. This book was released on 2011-11-22 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: This NATO Advanced Research Workshop held 25-30 September, 1988 at the Villa Gualino, Turin, Italy, was the first international meeting of its kind to be devoted solely to cell separation in plants. The partial or complete dissociation of one cell from another is an integral process of differentiation. Partial cell separations are basic physiological components of the overall programme of plant development. Complete cell separations are major events in the ripening of fruits, and the shedding of plant parts. Unscheduled cell separations commonly occur when tissues are subjected to pathogenic invasion. Environmental stresses too, evoke their own separation responses. Over the past five years much new knowledge has been acquired on the regulation of gene expression in specific stages of cell differentiation. Specific molecular markers have been identified that designate the competence of cells for achieving separation. Certain of the chemical signals (hormones, elicitors) that must be emitted or perceived by cells to initiate and sustain separation, are now known to us, and the resulting cell wall changes have come under close chemical scrutiny. The Turin meeting was a focus for those currently involved in such investigations. It assessed factors controlling cell separation in a wide spectrum of different cell types under a variety of conditions.
Download or read book Cell Separation written by Thomas G. Pretlow and published by . This book was released on 1982 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Subcellular Fractionation written by Paul R. Pryor and published by . This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods.
Download or read book The Molecular Biology of Plant Cells written by H. Smith and published by Univ of California Press. This book was released on 1977-01-01 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell structure and function; Gene expression and its regulation in plant cells; The manipulation of plant cells.
Download or read book Pathology of the Plant Cell written by Ernst Küster and published by . This book was released on 1996 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Comprehensive Sampling and Sample Preparation written by Josep M. Bayona and published by Academic Press. This book was released on 2012-12-31 with total page 2998 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comprehensive Sampling and Sample Preparation is a complete treatment of the theory and methodology of sampling in all physical phases and the theory of sample preparation for all major extraction techniques. It is the perfect starting point for researchers and students to design and implement their experiments and support those experiments with quality-reviewed background information. In its four volumes, fundamentals of sampling and sample preparation are reinforced through broad and detailed sections dealing with Biological and Medical, Environmental and Forensic, and Food and Beverage applications. The contributions are organized to reflect the way in which analytical chemists approach a problem. It is intended for a broad audience of analytical chemists, both educators and practitioners of the art and can assist in the preparation of courses as well in the selection of sampling and sample preparation techniques to address the challenges at hand. Above all, it is designed to be helpful in learning more about these topics, as well as to encourage an interest in sampling and sample preparation by outlining the present practice of the technology and by indicating research opportunities. Sampling and Sample preparation is a large and well-defined field in Analytical Chemistry, relevant for many application areas such as medicine, environmental science, biochemistry, pharmacology, geology, and food science. This work covers all these aspects and will be extremely useful to researchers and students, who can use it as a starting point to design and implement their experiments and for quality-reviewed background information There are limited resources that Educators can use to effectively teach the fundamental aspects of modern sample preparation technology. Comprehensive Sampling and Sample Preparation addresses this need, but focuses on the common principles of new developments in extraction technologies rather than the differences between techniques thus facilitating a more thorough understanding Provides a complete overview of the field. Not only will help to save time, it will also help to make correct assessments and avoid costly mistakes in sampling in the process Sample and sample preparation are integral parts of the analytical process but are often less considered and sometimes even completely disregarded in the available literature. To fill this gap, leading scientists have contributed 130 chapters, organized in 4 volumes, covering all modern aspects of sampling and liquid, solid phase and membrane extractions, as well as the challenges associated with different types of matrices in relevant application areas
Download or read book Density Gradient Centrifugation written by and published by Elsevier. This book was released on 1978-01-01 with total page 543 pages. Available in PDF, EPUB and Kindle. Book excerpt: Density Gradient Centrifugation
