Download or read book Appearance of Cellular and Humoral Immunity to Guinea Pigs Following Infection with Coxiella Burnetii Administered in Small Particle Aerosols written by Richard A. Kishimoto and published by . This book was released on 1976 with total page 19 pages. Available in PDF, EPUB and Kindle. Book excerpt: Development of humoral and cell-mediated immune responses was studied in guinea pigs infected with Coxiella burnetii administered in small-particle aerosols. Direct macrophage migration inhibition was observed in cultured peritoneal exudate cells as early as 3 days after exposure. Maximum inhibition of macrophages cultured with phase I or II antigen occurred 14 to 21 days post-exposure and persisted through 35 days. This inhibitory action was no longer detectable at 42 days. Serum antibody to the phase II antigen of C. burnetii was detected at 14 days and to phase I antigen at 21 days, 18 days after the cell-mediated immune response. (Author).
Download or read book Effect of Lysozyme on the Immune Response of Guinea Pigs to the Soluble Phase I Antigen of COXIELLA BURNETII written by Ralph F. Wachter and published by . This book was released on 1980 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pretreatment of guinea pigs with lysozyme prior to vaccination with the phase I antigen of Coxiella Burnetti enhanced antibody response and protection against challenge. An observed effect on macrophage migration suggests that the role of lysozyme includes stimulation of cell-mediated immunity. (Author).
Download or read book Comparative Analysis of Humoral and Cellular Immunity Induced by C Burnetii Whole Cell Vaccines written by Chen Chen and published by . This book was released on 2011 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: ABSTRACT: Coxiella burnetii is an obligate Gram-negative intracellular bacterium that causes acute Q fever and chronic infections in humans. An effective formalin killed whole cell vaccine (Q-Vax®), in use in Australia, is administered to individuals that are skin test and serologically negative. Vaccination can result in severe local or systemic adverse reaction, especially when administered to previously infected populations, and repeat vaccination can induce severe persistent reactions. Several other vaccines including inactivated Nine Mile I reference whole cell vaccines (Phase I-WCV) its derivative Phase II-W have been developed and tested in animal models. Previous study suggested that PI-WCV was more protective than PII-WCV against virulent bacterium challenge in a guinea pig and both humoral and cellular immunity are critical for vaccine derived C. burnetii protective immunity. In order to gain a better understanding of vaccine mediated protective immunity, we designed a series of experiments to dissect protective immune components through comparative analysis of PI-WCV and PII-WCV induced immune responses. First, by using C. burnetii protein microarray, we were able to decipher detailed IgG antibody profiles and make side-by-side comparison of humoral immune responses induced by PI-WCV and PII-WCV in mice. The overall IgG responding profiles were similar in these two vaccine induced sera and only identified differential immunodominant antigen was bacterial lipopolysaccharide (LPS). Although there were no major immunodominant protein antigen difference between PI-WCV and PII-WCV, we did see a higher overall IgG responses in PI-WCV immunization derived sera. Next, we analyzed the magnitude of CD4+ T cell responses induced by these two vaccines. By using newly identified C. burnetii H-2 I-A(superscript ♭) restricted T cell epitopes and several functional assays, we found that, comparing to PII-WCV, PI-WCV induced more cytokine producing CD4+ T cells (IFN-y, IL-2, and TNF-(alpha)) and these cells also had superior ability for secreting effector cytokines. Last, we compared APC responses upon stimulation with different vaccine antigens. Surprisingly, PI-WCV stimulated mBMDCs had more surface maturation marker CCR7 expression and better migratory ability than those induced by PII-WCV. In vivo functional assays further confirmed that better migration of PI-WCV treated mBMDCs correlated with more antigen presentation and T cell activation. These results may at least partially explain our observation, that higher humoral and cellular immunity are induced by PI-WCV immunization. Based on these results, we postulate that the PI-LPS or other undefined surface structure from phase I organism may trigger some unique host signaling pathway and serve as critical adjuvant for efficacious PI-WCV.
Download or read book Coxiella burnetii Recent Advances and New Perspectives in Research of the Q Fever Bacterium written by Rudolf Toman and published by Springer Science & Business Media. This book was released on 2012-06-19 with total page 413 pages. Available in PDF, EPUB and Kindle. Book excerpt: Coxiella burnetii is the etiological agent of Q fever, a zoonotic disease found worldwide. The bacterium is a fascinating example of intracellular parasitism that has uniquely evolved to thrive in the most inhospitable of cellular compartments-the phagolysosome. Understanding how C. burnetii resists the degradative functions of this vacuole, and the host cell functions coopted for successful parasitism, are central to understanding Q fever pathogenesis. Recent achievements in glycomics and proteomics are guiding development of enhanced detection schemes for the bacterium in addition to shedding light on the host immune response to the pathogen. Several chapters survey immune functions that control or potentially exacerbate Coxiella infection and delve into correlates of protective immunity elicited by vaccination. Comparative genomics is also the foundation of chapters discussing diagnostic antigen discovery and molecular typing of the bacterium, with significance for development of new clinical, epidemiologic, and forensic tools.
Download or read book Intracellular Parasitism written by James W. Moulder and published by CRC Press. This book was released on 2020-10-28 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: This publication is a collection of essays on the biology of intracellular parasitisms where both bacterial and protozoan parasites are discussed. The juxtaposition of authors representing fields of research emphasizes the many common problems facing intracellular parasites and the hosts that harbor them. In addition, numerous illustrations of how different parasites and host attempt to solve these problems in different ways are provided. The book includes one or more chapters on Bdellovibrio, Chlamydia, Rickettsia, Coxiella, Legionella, Shigellae, Mycobacterium, Microsporidium, Plasmodium, and Toxoplasma. The authors frequently speculate and generalize on the subject matter discussed.
Download or read book Microorganisms and Bioterrorism written by Burt Anderson and published by Springer Science & Business Media. This book was released on 2007-05-26 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this book is to bring together, in a single volume, the most up-to-date information concerning microbes with potential as bioterrorist weapons. The primary audience includes microbiologists, including bacteriologists, virologists and mycologists, in academia, government laboratories and research institutes at the forefront of studies concerning microbes which have potential as bioterrorist weapons, public health physicians and researchers and scientists who must be trained to deal with bioterrorist attacks as well as laboratory investigators who must identify and characterize these microorganisms from the environment and from possibly infected patients.
Download or read book Annual Progress Report written by and published by . This book was released on 1978 with total page 538 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Annual Progress Report written by U.S. Army Medical Research Institute of Infectious Diseases and published by . This book was released on 1978 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1989 with total page 1422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Annual Progress Report U S Army Medical Research Institute of Infectious Diseases written by U.S. Army Medical Research Institute of Infectious Diseases and published by . This book was released on 1976 with total page 644 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Journal of Immunology written by and published by . This book was released on 2007 with total page 684 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The BALB c Mouse written by Michael Potter and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: The mouse was first used in immunological research by Paul Ehrlich in 1891 in an extraordinary series of experiments on the maternal transfer of antitoxic immunity. A short 22 years later in 1913 Halsey Bagg acquired a stock of albino mice from a commercial dealer and used them in a series of experiments on learning. Because he was interested in the genetics of intelligence, Halsey Bagg began breeding a pedigreed line of these mice that were subsequently named for him - Bagg Albino. Though Halsey Bagg is not credited with initiating the inbred strains of mice, his stock curiously has played an indisputably important role. Bagg Albinos were progenitors of the present day BALB/c family of sublines - the subject of this book. They were also used as one of the parents in the development of inbred strains A, CBA and C3H, three other very famous strains. Today the BALB/c mouse is among the five most widely used inbred strains in biomedical research and a particular favorite in immunology and infectious disease research. The hallmark of the BALB/c response to so many kinds of infections is susceptibility and sometimes an exaggerated susceptibility, but this paradoxically is not associated with immunodeficiency as BALB/c is an excellent responder to immuni zation. These characteristics have made the BALB/c mouse a model for identifying genes that determine susceptibility to infectious and neoplastic diseases. In 1985 the laboratory BALB/c mouse became 72 years old. The current filial generations are somewhere around 350 generations [MURPHY].
Download or read book Q Fever written by Thomas J. Marrie and published by CRC Press. This book was released on 1990-07-03 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fascinating book describes the history, and epidemiology in both man and animals, and clinical features of this zoonosis. It reveals detailed information pertaining to the basic science aspects of the interaction of Coxiella burnetii, the etiologic agent of Q (Query) fever, with the host and the immune response of the host to this unique pathogen. This one-of-a-kind work uses the rules of epidemiological evidence to determine whether or not Coxiella burnetii is a perinatal pathogen for man. This interesting, easy-to-understand text concludes with chapters dealing with the antibiotic susceptibility of Coxiella burnetii and the studies that resulted in a vaccine that now can be used to prevent this infection. This volume is an absolute must for all microbiologists, respirologists, infectious disease physicians and veterinarians.
Download or read book Canadian Journal of Microbiology written by and published by . This book was released on 1979 with total page 796 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Journal of General Microbiology written by and published by . This book was released on 1987 with total page 658 pages. Available in PDF, EPUB and Kindle. Book excerpt: Contains abstracts of papers presented at meeting of the Society for General Microbiology.
Download or read book Abstracts of microbiology and hygiene written by and published by . This book was released on 1979 with total page 722 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Q Fever written by Jim C. Williams and published by CRC Press. This book was released on 1991-10-23 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: Q fever is a zoonotic infectious disease caused by Coxiella burnetii, which infects a broad range of hosts. This volume presents critical reviews of the biological aspects of C. burnetii. Topics covered include C. burnetii's place in the microbial world through a comparison with other microorganisms that are phylogenetically related or share phenotypic traits; intrinsic properties of Coxiella with respect to infectivity, virulence, and pathogenecity; past and current methods for the isolation, amplification, and purification of C. Burnetii; obligate acidophily; metabolic capabilities, with emphasis on intermediary metabolism, macromolecular synthesis, and protein export; and human immune responses to bacterial components during acute and chronic infection by C. Burnetii. Other fascinating topics about C. Burnetii include antigens, virulence factors, and biological response modifiers; ultrastructure; genetics; cloning and expression; and C. burnetii in the U.S.S.R. and China. Epidemiologists, molecular biologists, microbiologists, and other researchers interested in Q fever and C. burnetii should consider this book an essential reference resource.
