Download or read book Cell Free Nucleic Acids written by Bálint Nagy and published by MDPI. This book was released on 2020-01-21 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: The deficits of mammography and the potential of noninvasive diagnostic testing using circulating miRNA profiles are presented in our first review article. Exosomes are important in the transfer of genetic information. The current knowledge on exosome-associated DNAs and on vesicle-associated DNAs and their role in pregnancy-related complications is presented in the next article. The major obstacle is the lack of a standardized technique for the isolation and measurement of exosomes. One review has summarized the latest results on cell-free nucleic acids in inflammatory bowel disease (IBD). Despite the extensive research, the etiology and exact pathogenesis are still unclear, although similarity to the cell-free ribonucleic acids (cfRNAs) observed in other autoimmune diseases seems to be relevant in IBD. Liquid biopsy is a useful tool for the differentiation of leiomyomas and sarcomas in the corpus uteri. One manuscript has collected the most important knowledge of mesenchymal uterine tumors and shows the benefits of noninvasive sampling. Microchimerism has also recently become a hot topic. It is discussed in the context of various forms of transplantation and transplantation-related advanced therapies, the available cell-free nucleic acid (cfNA) markers, and the detection platforms that have been introduced. Ovarian cancer is one of the leading serious malignancies among women, with a high incidence of mortality; the introduction of new noninvasive diagnostic markers could help in its early detection and treatment monitoring. Epigenetic regulation is very important during the development of diseases and drug resistance. Methylation changes are important signs during ovarian cancer development, and it seems that the CDH1 gene is a potential candidate for being a noninvasive biomarker in the diagnosis of ovarian cancer. Preeclampsia is a mysterious disease—despite intensive research, the exact details of its development are unknown. It seems that cell-free nucleic acids could serve as biomarkers for the early detection of this disease. Three research papers deal with the prenatal application of cfDNA. Copy number variants (CNVs) are important subjects for the study of human genome variations, as CNVs can contribute to population diversity and human genetic diseases. These are useful in NIPT as a source of population specific data. The reliability of NIPT depends on the accurate estimation of fetal fraction. Improvement in the success rate of in vitro fertilization (IVF) and embryo transfer (ET) is an important goal. The measurement of embryo-specific small noncoding RNAs in culture media could improve the efficiency of ET.
Download or read book Cell Free Nucleic Acids written by Bálint Nagy and published by . This book was released on 2020 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: The deficits of mammograp ...
Download or read book Cell free DNA as Diagnostic Markers written by Valentina Casadio and published by Humana Press. This book was released on 2019-02-04 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the most important techniques used for studying cfDNA in the different samples; serum, plasma, urine. Chapters detail methods on liquid biopsy for cancer disease, methods in cancer, epigenetic modifications, fetal and pediatric diseases, physical activity, and urinary cell free DNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Free DNA as Diagnostic Markers: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Download or read book Circulating Nucleic Acids in Serum and Plasma CNAPS IX written by Peter B. Gahan and published by Springer. This book was released on 2016-10-17 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids. There are a modest number of laboratories involved in this field, nevertheless the number of papers published by researchers is extensive. The articles which will be published in this meeting report will be a valuable contribution for researchers and research students alike and will help them to stay on top of the developments in different research areas and to „cross borders“ between them.
Download or read book Genomic Medicine written by Laura J. Tafe and published by Springer Nature. This book was released on 2019-09-26 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of Molecular Diagnostics is rapidly evolving and molecular characterization of neoplasms is becoming an increasingly important part of the pathologic work up and diagnosis of many tumor types. This work provides a high-yield reference book that compiles critical information related to molecular biomarkers for various solid tumor and hematologic malignancy subtypes. It is succinct yet comprehensive enough to be suitable for fellows in training and medical professionals with an interest in molecular pathology and biomarkers. The book covers many aspects of molecular diagnostics, from techniques to applications and comprehensive summaries of the current molecular biomarkers of critical importance in solid and liquid tumors. Attention is also specifi cally devoted to bioinformatics and next generation sequencing, as well as pre-analytical issues that must be considered for accurate interpretation of molecular results in the context of overall patient care. This text focuses on clinical utility and validity and serves as an “owner’s manual” in Genomic Diagnostics for the practicing pathologist, pathology fellows and residents and other health care providers. Physicians will find this book invaluable as a quick reference for current molecular testing modalities and guidelines, tumor board preparation, deciding which test to order and interpreting genomic laboratory results. In addition, it is an accessible for trainees as a board review preparation reference.
Download or read book Circulating Nucleic Acids in Early Diagnosis Prognosis and Treatment Monitoring written by Peter B. Gahan and published by Springer. This book was released on 2014-11-18 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA and RNA fractions can be isolated from a variety of body fluids including whole blood, serum, plasma, urine, saliva and cerebrospinal fluid from both patients and healthy individuals. Such isolates can be exploited in the early detection of clinical disorders, stratification of patients for treatment, treatment monitoring and clinical follow-up. In addition, the use in fetal medicine allows the early detection of fetal sex, Rh factor and aneuploid disorders as well as following both fetal and premature born infant development. This volume is intended as a primer for those who are interested in entering the field of circulating nucleic acids. The areas covered in this volume include: · Background and general biology of circulating nucleic acids · Methodology · Applications of circulating nucleic acids · Quality Assurance · Ethics
Download or read book Understanding DNA written by Chris R. Calladine and published by Elsevier. This book was released on 2004-03-13 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: The functional properties of any molecule are directly related to, and affected by, its structure. This is especially true for DNA, the molecular that carries the code for all life on earth. The third edition of Understanding DNA has been entirely revised and updated, and expanded to cover new advances in our understanding. It explains, step by step, how DNA forms specific structures, the nature of these structures and how they fundamentally affect the biological processes of transcription and replication. Written in a clear, concise and lively fashion, Understanding DNA is essential reading for all molecular biology, biochemistry and genetics students, to newcomers to the field from other areas such as chemistry or physics, and even for seasoned researchers, who really want to understand DNA. Describes the basic units of DNA and how these form the double helix, and the various types of DNA double helix Outlines the methods used to study DNA structure Contains over 130 illustrations, some in full color, as well as exercises and further readings to stimulate student comprehension
Download or read book Precision Medicine in Oncology written by Bulent Aydogan and published by John Wiley & Sons. This book was released on 2020-11-02 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: A FRESH EXAMINATION OF PRECISION MEDICINE'S INCREASINGLY PROMINENT ROLE IN THE FIELD OF ONCOLOGY Precision medicine takes into account each patient's specific characteristics and requirements to arrive at treatment plans that are optimized towards the best possible outcome. As the field of oncology continues to advance, this tailored approach is becoming more and more prevalent, channelling data on genomics, proteomics, metabolomics and other areas into new and innovative methods of practice. Precision Medicine in Oncology draws together the essential research driving the field forward, providing oncology clinicians and trainees alike with an illuminating overview of the technology and thinking behind the breakthroughs currently being made. Topics covered include: Biologically-guided radiation therapy Informatics for precision medicine Molecular imaging Biomarkers for treatment assessment Big data Nanoplatforms Casting a spotlight on this emerging knowledge base and its impact upon the management of tumors, Precision Medicine in Oncology opens up new possibilities and ways of working – not only for oncologists, but also for molecular biologists, radiologists, medical geneticists, and others.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Epigenetic Cancer Therapy written by Steven Gray and published by Academic Press. This book was released on 2015-07-01 with total page 748 pages. Available in PDF, EPUB and Kindle. Book excerpt: Epigenetic Cancer Therapy unites issues central to a translational audience actively seeking to understand the topic. It is ideal for cancer specialists, including oncologists and clinicians, but also provides valuable information for researchers, academics, students, governments, and decision-makers in the healthcare sector. The text covers the basic background of the epigenome, aberrant epigenetics, and its potential as a target for cancer therapy, and includes individual chapters on the state of epigenome knowledge in specific cancers (including lung, breast, prostate, liver). The book encompasses both large-scale intergovernmental initiatives as well as recent findings across cancer stem cells, rational drug design, clinical trials, and chemopreventative strategies. As a whole, the work articulates and raises the profile of epigenetics as a therapeutic option in the future management of cancer. Concisely summarizes the therapeutic implications of recent, large-scale epigenome studies, including the cancer epigenome atlas Discusses targeted isoform specific versus pan-specific inhibitors, a rational drug design approach to epigenetics relevant to pharmacoepigenetic clinical applications Covers new findings in the interplay between cancer stem cells (CSCs) and drug resistance, demonstrating that epigenetic machinery is a candidate target for the eradication of these CSCs
Download or read book Biological Sequence Analysis written by Richard Durbin and published by Cambridge University Press. This book was released on 1998-04-23 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: Probabilistic models are becoming increasingly important in analysing the huge amount of data being produced by large-scale DNA-sequencing efforts such as the Human Genome Project. For example, hidden Markov models are used for analysing biological sequences, linguistic-grammar-based probabilistic models for identifying RNA secondary structure, and probabilistic evolutionary models for inferring phylogenies of sequences from different organisms. This book gives a unified, up-to-date and self-contained account, with a Bayesian slant, of such methods, and more generally to probabilistic methods of sequence analysis. Written by an interdisciplinary team of authors, it aims to be accessible to molecular biologists, computer scientists, and mathematicians with no formal knowledge of the other fields, and at the same time present the state-of-the-art in this new and highly important field.
Download or read book Metal Enhanced Fluorescence written by Chris D. Geddes and published by John Wiley & Sons. This book was released on 2010-06-22 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.
Download or read book Precision Medicine for Investigators Practitioners and Providers written by Joel Faintuch and published by Academic Press. This book was released on 2019-11-16 with total page 640 pages. Available in PDF, EPUB and Kindle. Book excerpt: Precision Medicine for Investigators, Practitioners and Providers addresses the needs of investigators by covering the topic as an umbrella concept, from new drug trials to wearable diagnostic devices, and from pediatrics to psychiatry in a manner that is up-to-date and authoritative. Sections include broad coverage of concerning disease groups and ancillary information about techniques, resources and consequences. Moreover, each chapter follows a structured blueprint, so that multiple, essential items are not overlooked. Instead of simply concentrating on a limited number of extensive and pedantic coverages, scholarly diagrams are also included. Provides a three-pronged approach to precision medicine that is focused on investigators, practitioners and healthcare providers Covers disease groups and ancillary information about techniques, resources and consequences Follows a structured blueprint, ensuring essential chapters items are not overlooked
Download or read book Cell Free Synthetic Biology written by Jian Li and published by Frontiers Media SA. This book was released on 2022-01-13 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Exosomes Stem Cells and MicroRNA written by Karl L. Mettinger and published by Springer. This book was released on 2018-05-12 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides insight into the pivotal roles of stem cells, exosomes and other microvesicles in biofunction and molecular mechanisms and their therapeutic potential in translational nanomedicine. It further highlights evidence from recent studies as to how stem cell derived exosomes and microRNAs may restore and maintain tissue homeostasis, enable cells to recover critical cellular functions and begin repair regeneration. These early studies in animal models of aging also show evidence of improved immune, cardiovascular and cognitive functions as well as improved health span and life span. The use of exosomes from body fluids to define specific biomarkers for various tumors may also clear the path to patient-targeted treatments by developing exosome-derived microRNA based cancer therapeutics. It is essential reading for graduate students, research fellow and biomedical researchers in academia or the pharmaceutical or biotech industries.
Download or read book Circulating Nucleic Acids in Plasma and Serum written by Peter B. Gahan and published by Springer Science & Business Media. This book was released on 2010-10-08 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA and RNA fractions have been isolated from the whole blood, serum, plasma, the surface of blood cells, urine, saliva and spinal fluid from both healthy individuals and clinical patients. Recent developments are presented concerning the isolation, quantification and analysis of these molecules and their use in the identification of specific nucleic acid fragments related to a variety of clinical disorders thereby permitting their early diagnosis and prognosis.
Download or read book Liquid Biopsy written by Ilze Strumfa and published by BoD – Books on Demand. This book was released on 2019-07-10 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reliable diagnosis is the cornerstone, starting point, and prerequisite of successful treatment. Therefore, development of innovative diagnostic technologies represents a hot topic in medical research. Liquid biopsy is a novel, minimally invasive laboratory evaluation concept for diagnostic, prognostic, and predictive testing, as well as dynamic monitoring of treatment or disease course. To achieve these goals, a multitude of specific, targeted tests can be performed to detect free nucleic acids, exosomes, microRNAs, tumor-educated platelets, and whole cells of tumor or fetal origin in different biological fluids, including blood, urine, cerebrospinal fluid, and others. Although tissue biopsy has long been considered the gold standard of diagnostics, especially regarding malignant tumors, liquid biopsy has the advantages of a non-invasive approach and thus low risk of complications. It is technically feasible even in serious general status or if tumors or metastases are not easily accessible using conventional tissue biopsy. The testing is fast, exact, and can be repeated to ensure real-time follow-up. In contrast to classic tumor markers, liquid biopsy is distinguished by high specificity at genomic, proteomic, and cellular levels. It is expected to equal and exceed the diagnostic value of tissue biopsy. The field of liquid biopsies is developing rapidly regarding the selection of targets, technological improvements, and quality assessment. This book, written by a global team of recognized scientists, comprises state-of-the-art reviews on the current knowledge and advances in the technologies and software for liquid biopsy. Examples of practical application of liquid biopsy to evaluate thyroid cancer, multiple myeloma, etc. are discussed as well. The book is intended to serve as a reference for scientists and clinicians interested in the development and practical implementation of liquid biopsy.
