Download or read book Cell Culture Approaches to Invertebrate Neuroscience written by George Lees and published by . This book was released on 1988 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Culture in the Neurosciences written by Jane Bottenstein and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: A fundamental problem in neuroscience is the elucidation of the cellular and molecular mechanisms underlying the development and function of the nervous system. The complexity of organization, the heteroge neity of cell types and their interactions, and the difficulty of controlling experimental variables in intact organisms make this a formidable task. Because of the ability that it affords to analyze smaller components of the nervous system (even single cells in some cases) and to better control experimental variables, cell culture has become an increasingly valuable tool for neuroscientists. Many aspects of neural development, such as proliferation, differentiation, synaptogenesis, and myelination, occur in culture with time courses remarkably similar to those in vivo. Thus, in vitro methods often provide excellent model systems for investigating neurobiological questions. Ross Harrison described the first culture of neural tissue in 1907 and used morphological methods to analyze the cultures. Since that time the technique has been progressively modified and used to address an ever widening range of developmental questions. In recent years a con vergence of new or improved cell culture, biochemical, electrophysiol ogical, and immunological methods has occurred and been brought to bear on neurobiological questions. This volume is intended not to be comprehensive but rather to highlight some of the latest findings, with a review of previous important work as well, in which combinations of these methods are used.
Download or read book Cell Culture Techniques written by Michael Aschner and published by Humana Press. This book was released on 2011-03-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Societal, ethical, and cost-related issues, not to mention the need for sound scientific methods, have led to new and refined methods for the evaluation of health risks associated with neurotoxic compounds, relevant and predictive of exposure, relatively inexpensive, and ideally amenable to high throughput analysis and a reduction in animal use. Cell Culture Techniques presents thorough traditional chapters, such as those on various cell culture methods that have evolved over the years, as well as innovative approaches to neurotoxicologic testing. Accordingly, this detailed volume describes how stem cells, computational biology, and other novel powerful methods can now be applied to address the challenges of neurotoxic testing. As part of the Neuromethods series, this work provides the kind of intensive description and implementation advice that is crucial for getting optimal results in the laboratory. Practical and authoritative, Cell Culture Techniques serves both the novice and the experienced neurotoxicologist by inspiring the further development of mechanistically-driven, cost-effective, high throughput series of tests needed to meet the many contemporary challenges.
Download or read book Neuronal Cell Culture written by Shohreh Amini and published by Humana. This book was released on 2021-05-26 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.
Download or read book Neurons Methods and Applications for the Cell Biologist written by and published by Elsevier. This book was released on 2003-10-02 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented. - Contains culture methodology for more than a dozen types of CNS and PNS neurons - Includes most recent and reliable techniques from expert practitioners for specific experimental applications - Addresses the latest strategies for transfecting neurons
Download or read book The Neuron in Tissue Culture written by L. W. Haynes and published by . This book was released on 1999-02-22 with total page 780 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume in the Handbook Series contains 73 contributions from experts in the range of fields to which neuronal tissue culture may be applied. Twenty-two keynote chapters contain methods, well-illustrated with data from the contributing authors? laboratones, which exemplify ?landmark? applications of the technique. Three further tutorial chapters review artefacts of the tissue culture environment, the understanding and control of neuronal apoptosis in vitro, and the culture substratum. The volume concludes with five chapters containing short protocols describing systematically the maintenance and propagation of neurons from mammalian and avian peripheral and central nervous systems, stem cell populations, invertebrate neurons, and finally selected organised and reconstruction techniques which are given prominent treatment throughout the book. The Neuron in Tissue Culture is a comprehensive reference source dealing exclusively with the tissue culture of neurons. It is essential reading for all those with research interests in neuroscience, including developmental biologists, neurologists, nerve cell physiologists and neuropharmaoologists.
Download or read book Methods for Serum free Culture of Neuronal and Lymphoid Cells written by David Andrew Sirbasku and published by . This book was released on 1984 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protocols for Neural Cell Culture written by Laurie C. Doering and published by Humana Press. This book was released on 2009-10-29 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies. The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions’ tradition of exceptional quality and thorough coverage in tissue culture protocols for today’s neuroscience in order to inspire researchers in the field to further explore this imperative area of study.
Download or read book Culturing Nerve Cells written by Gary Banker and published by MIT Press (MA). This book was released on 1991 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: This do-it-yourself manual describes complete recipes and protocols for biological scientists, particularly neurobiologists, who want to learn how to grow neurons in culture and how to do it well. Moreover, it provides an understanding of the principles behind the protocols. Offering a more coherent overview of techniques than the usual compendium of methods sections, Culturing Nerve Cells also takes into account the numerous details that can make the difference between success and failure by asking experienced culturists to share their technical expertise. Introductory chapters present a brief course on the nuts and bolts of culturing all types of nerve cells—including the kinds of cultures available and which might be the best for a specific application—methods for cell dissociation, culture media, and substrates, and a description of the techniques needed to characterize and study cultures. More than 20 experts then describe the culture systems that they have developed, including the details of each protocol as well as all of the tricks, lore, and troubleshooting techniques that never appear in the scientific literature. Contents: A User's Guide. Getting Started. General Principles. Types of Nerve Cell Cultures, Their Advantages and Limitations. Primary Dissociated Cell Cultures of Neuronal Tissue. Characterizing and Studying Neuronal Cultures. Culture of Specific Cell Types. Isolated Chick Neurons for the Study of Axonal Growth. Culturing Spinal Neurons and Muscle Cells from Xenopus Embryos. Culturing the Large Neurons of Aplysia. Tissue Culture of Mammalian Autonomic Neurons. Methodologies for the Culture and Experimental Use of the PC12 Rat Pheochromocytoma Cell Line. Cell Culture of Neocortex and Basal Forebrain from Postnatal Rats. Rat Hippocampal Neurons in Low-Density Culture. Cerebellar Cells in Culture. Astroglia in Culture. Tissue Culture Methods for the Study of Myelination. Organotypic Slice Cultures of Neural Tissue.
Download or read book Neuroscience Approached Through Cell Culture written by Steven E. Pfeiffer and published by . This book was released on 1982 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Culture written by P. Michael Conn and published by . This book was released on 1990 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to assess the response of neural cells is an important goal of research in the neurosciences. The means to measure responses in a chemically defined medium is a positive feature of the use of cell culture and is the major reason its use is so important in this discipline. Techniques for the purification of cells for culture, for large-scale isolation, for assessing differentiated function, and for tracing lineage are included. Many of the methods can easily be adapted to other systems. Key Features * Convenient benchtop format * Methods presented for easy adaptation to new systems * Comprehensive protocols included for * Preparation and maintenance of primary and continuous cultures * Cell purification * Bulk isolation * Studying pulsatility, growth on immobile carriers, rotation-mediated aggregation, microcultures, Maximow assembly cultures * Assessment of differentiated and functional aspects: endocrine modulation, morphological variants, receptors, intracellular trafficking * Clonal analysis and lineage analysis
Download or read book Invertebrate Tissue Culture written by Noriaki Agui and published by . This book was released on 1976 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Aquatic Invertebrate Cell Culture written by Carmel Mothersill and published by Springer. This book was released on 2000-09-25 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aquatic Invertebrate Cell Culture is a very new field which has major applications in Aquaculture, Ecotoxicology and Pathology. In essence it is realised that pathogens behave differently in host cells than the impression gained from growth on agar plates. Another major application of invertebrate cell culture is an understanding of mechanisms involved in cellular and molecular responses to environmental change. This book aims to consider all relevant advances for the development of aquatic invertebrate cell culture.
Download or read book Cellular and Molecular Methods in Neuroscience Research written by Adalberto Merighi and published by . This book was released on 2000-12 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are numerous books on cellular and molecular protocols for general use in cell biology but very few are exclusively devoted to neurobiology. This book fills this gap and explains in a clear and consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.
Download or read book Guide to Research Techniques in Neuroscience written by Matt Carter and published by Academic Press. This book was released on 2022-03-26 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. - Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods - Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more - Clear, straightforward explanations of each technique for anyone new to the field - A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture - Detailed recommendations on where to find protocols and other resources for specific techniques - "Walk-through" boxes that guide readers through experiments step-by-step
Download or read book Nervous System Regeneration in the Invertebrates written by Stacia B. Moffet and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt: Invertebrate animals represent a diversity of solutions to life's challenges. Success in a wide range of environments has been achieved by an almost bewildering range of invertebrate body forms. These body forms are reflected in the wonderful diversity of their nervous systems. Despite this apparent diversity, studies of the development of invertebrates and vertebrates are yielding common themes at the molecular level. Likewise, the phenome non of neural regeneration is based upon properties intrinsic to neurons and responses to a remarkably conserved chemical lan guage. This monograph focuses on the diversity and commonal ity of responses to neural injury. The rough and tumble of life may frequently damage some part of the body, particularly the appendages or sensory sys tems. The nervous system is usually involved in repair of other body systems and often may itself require repair. Some animals are particularly successful in regenerating the nervous system or body parts. We particularly marvel at these feats of regeneration because we human beings are not particularly successful, despite our relatively long life and the advantages that would seem to accrue from such repair. It is no wonder that we would hope to learn the secrets of the more successful animals and strive to emulate them! Mechanisms of neural regeneration are often more acces sible in invertebrates than in vertebrates because questions of specificity are more easily addressed using the identifiable neu rons of the relatively simpler nervous systems of some inverte brates.
Download or read book The Oxford Handbook of Invertebrate Neurobiology written by John H. Byrne and published by Oxford University Press. This book was released on 2019-01-29 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Invertebrates have proven to be extremely useful model systems for gaining insights into the neural and molecular mechanisms of sensory processing, motor control and higher functions such as feeding behavior, learning and memory, navigation, and social behavior. A major factor in their enormous contributions to neuroscience is the relative simplicity of invertebrate nervous systems. In addition, some invertebrates, primarily the molluscs, have large cells, which allow analyses to take place at the level of individually identified neurons. Individual neurons can be surgically removed and assayed for expression of membrane channels, levels of second messengers, protein phosphorylation, and RNA and protein synthesis. Moreover, peptides and nucleotides can be injected into individual neurons. Other invertebrate model systems such as Drosophila and Caenorhabditis elegans offer tremendous advantages for obtaining insights into the neuronal bases of behavior through the application of genetic approaches. The Oxford Handbook of Invertebrate Neurobiology reviews the many neurobiological principles that have emerged from invertebrate analyses, such as motor pattern generation, mechanisms of synaptic transmission, and learning and memory. It also covers general features of the neurobiology of invertebrate circadian rhythms, development, and regeneration and reproduction. Some neurobiological phenomena are species-specific and diverse, especially in the domain of the neuronal control of locomotion and camouflage. Thus, separate chapters are provided on the control of swimming in annelids, crustaea and molluscs, locomotion in hexapods, and camouflage in cephalopods. Unique features of the handbook include chapters that review social behavior and intentionality in invertebrates. A chapter is devoted to summarizing past contributions of invertebrates to the understanding of nervous systems and identifying areas for future studies that will continue to advance that understanding.
