Download or read book Catalytic RNA written by Fritz Eckstein and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, unprecedented advances in many aspects of the molecular biology of nucleic acids have been witnessed. The area of RNA chemistry has undergone a kind of explosion, with a huge interest in RNA-mediated catalysis. At the same time, our structural understanding of DNA-protein interactions has increased enormously, and the related area of RNA-protein interactions is beginning to gather pace. This softcover edition from the successful series Nucleic Acids and Molecular Biology is devoted to the structure and mechanism of ribozymes, and their potential exploitation. The subject has both important evolutionary implications and potential practical application in the development of therapeutic agents for diseases such as AIDS.
Download or read book Ribozymes and RNA Catalysis written by David Malcolm James Lilley and published by Royal Society of Chemistry. This book was released on 2008 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: Takes the reader through the origins of catalysis in RNA and necessarily includes significant discussion of structure and folding. The main focus of the book concerns chemical mechanism with extensive comment on how, despite the importance of RNA catalysis in the cell, its origins are still poorly understood and often controversial. The reader is given an outline of the important role of RNA catalysis in many aspects of cell function, including RNA processing and translation.
Download or read book Catalytic RNA written by and published by Academic Press. This book was released on 2013-10-21 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: This special volume of Progress in Molecular Biology and Translational Science focuses on catalytic RNA. Written by experts in the field, the reviews cover a range of topics, from hammerhead ribozymes to spliceosome catalysis to Varkud satellite and hairpin ribozymes. Contributions from leading authorities Informs and updates on all the latest developments in the field
Download or read book Looking at Ribozymes written by Benoît Masquida and published by John Wiley & Sons. This book was released on 2024-04-02 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: Behind the neologism “ribozymes” lies a family of fascinating molecules, ribo-enzymes, which have been relatively little studied. These catalytically active RNAs are found in all strata of life, from viruses to the human genome. At the end of the 1970s, the discovery of a catalytic RNA nestled in an intron, followed by another involved in the maturation of transfer RNAs, led to the discovery of new ribozymes and the transition from a strictly “proteocentric” vision, inherited from the dogma of molecular biology, to a more “nucleocentric” one. Since then, a variety of ribozymes have been identified in genomes, where their functions often remain mysterious. Looking at Ribozymes traces the discovery of these molecules and presents a picture of their functional diversity, catalytic mechanisms and distribution within the tree of life.
Download or read book Ribozymes written by Sabine Müller and published by John Wiley & Sons. This book was released on 2021-07-09 with total page 81 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ribozymes Provides comprehensive coverage of a core field in the molecular biosciences, bringing together decades of knowledge from the world’s top professionals in the field Timely and unique in its breadth of content, this all-encompassing and authoritative reference on ribozymes documents the great diversity of nucleic acid-based catalysis. It integrates the knowledge gained over the past 35 years in the field and features contributions from virtually every leading expert on the subject. Ribozymes is organized into six major parts. It starts by describing general principles and strategies of nucleic acid catalysis. It then introduces naturally occurring ribozymes and includes the search for new catalytic motifs or novel genomic locations of known motifs. Next, it covers the development and design of engineered ribozymes, before moving on to DNAzymes as a close relative of ribozymes. The next part examines the use of ribozymes for medicinal and environmental diagnostics, as well as for therapeutic tools. It finishes with a look at the tools and methods in ribozyme research, including the techniques and assays for structural and functional characterization of nucleic acid catalysts. The first reference to tie together all aspects of the multi-faceted field of ribozymes Features more than 30 comprehensive chapters in two volumes Covers the chemical principles of RNA catalysis; naturally occurring ribozymes, engineered ribozymes; DNAzymes; ribozymes as tools in diagnostics and therapy, and tools and methods to study ribozymes Includes first-hand accounts of concepts, techniques, and applications by a team of top international experts from leading academic institutions Dedicates half of its content to methods and practical applications, ranging from bioanalytical tools to medical diagnostics to therapeutics Ribozymes is an unmatched resource for all biochemists, biotechnologists, molecular biologists, and bioengineers interested in the topic.
Download or read book Structural and Catalytic Roles of Metal Ions in RNA written by Astrid Sigel and published by Walter de Gruyter GmbH & Co KG. This book was released on 2015-07-24 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: The discovery of ribozymes nearly 30 years ago triggered a huge interest in the chemistry and biology of RNAs. Much of the recently made progress focusing on metal ions is addressed in MILS 9. This book, written by 28 internationally recognized experts from 8 nations, provides a most up-to-date view and is thus of special relevance for colleagues teaching courses in biological inorganic chemistry and for researchers dealing, e.g., with nucleic acids, gene expression, and enzymology, but also for those in analytical and bioinorganic chemistry or biophysics. Structural and Catalytic Roles of Metal Ions in RNA describes in an authoritative and timely manner in 12 stimulating chapters, supported by nearly 1600 references, 13 tables, and 75 illustrations, mostly in color, metal ion-binding motifs, methods to detect and characterize metal ion-binding sites, and the role of metal ions in folding and catalysis. It deals with diffuse metal ion binding, RNA quadruplexes, the regulation of riboswitches, metal ions and ribozymes, including artificial ribozymes. The spliceosome, the ribosome, ribozymes involving redox cofactors as well as the binding of kinetically inert metal ions to RNA are also covered.
Download or read book Small Catalytic RNA written by Joseph Albert Monforte and published by . This book was released on 1991 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structural and Catalytic Roles of Metal Ions in RNA written by Astrid Sigel and published by Royal Society of Chemistry. This book was released on 2011 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: The discovery of ribozymes triggered a huge interest in the chemistry and biology of RNAs. Much of the recently made progress focusing on metal ions is addressed in Volume 9. This book, written by 28 internationally recognized experts, provides a most up-to-date view and it is thus of special relevance for colleagues teaching courses in biological inorganic chemistry and for researchers dealing, e.g., with nucleic acids, gene expression, and enzymology, but also for those in analytical and bioinorganic chemistry or biophysics. Structural and Catalytic Roles of Metal Ions in RNA describes metal ion-binding motives, methods to detect and characterize metal ion binding sites, and the role of metal ions in folding and catalysis. It deals with diffuse metal ion binding, RNA quadruplexes, the regulation of riboswitches, metal ions and ribozymes, including artificial ribozymes. The ribosome, ribozymes and redox cofactors, as well as the binding of kinetically inert metal ions to RNA are also considered.
Download or read book Catalytic RNAs for a RNA World written by Janina E. Moretti and published by . This book was released on 2013 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: At some point in the origin of life, life likely went through a stage where RNA acted as genome and catalyst--the RNA world. Eventually, life evolved into the form we observe today, with DNA acting as genome and proteins acting as the main catalysts. Although there is no known direct evidence of RNA world organisms, we can gain understanding of life's early ancestors by creating catalytic RNAs in the lab that could have existed in an RNA world. This dissertation aims to develop and improve two of these catalytic RNAs (ribozymes). The first is a triphosphorylation ribozyme, a catalytic RNA that triphosphorylates the 5' hydroxyl groups of RNA using trimetaphosphate. In a RNA world, this activity could have been useful for chemically activating RNAs for ligation or polymerization. The second is a polymerase ribozyme, a catalytic RNA that catalyzes RNA polymerization. This activity could have been important for replication in a RNA world organism. In order to identify catalytic RNAs that can triphosphorylate their own 5' hydroxyl groups, an in vitro selection method was established. From a library of ~1014 random sequences, several active sequences were identified and one was analyzed in greater detail. This triphosphorylation ribozyme was modified to act in trans and had a reaction rate of 0.16 min−1 under optimal conditions. Preliminary analysis of its secondary structure suggests that it forms a four helical junction motif. A catalytic RNA that catalyzes the polymerization of RNA was previously developed, but it is too inefficient to replicate itself. Its limitation is weak substrate binding. Here efforts were made to improve the polymerase ribozyme by reducing charge repulsion between the negatively charged ribozyme and its substrate using positively charged amino acids as cofactors. However, these cofactors did not improve ribozyme polymerization. An in vitro selection method that puts selection pressure on the ribozyme to bind its substrate in trans was developed to try to find improved polymerase ribozymes. Although no active ribozymes were found, several steps of a technically challenging in vitro selection method were established. In summary, this thesis presents work to develop two catalytic RNAs for a RNA world. An existing polymerase ribozyme was tested with amino acid cofactors and progress was made towards developing an in vitro selection that could be used to find novel polymerase ribozymes. Also, a novel catalytic RNA that can triphoshphorylate RNAs was created. This opens up new avenues that will deepen our understanding of the RNA world.
Download or read book Ribozymes and RNA Catalysis written by David M J Lilley and published by Royal Society of Chemistry. This book was released on 2007-11-15 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: The discovery that RNA could act as a macromolecular catalyst in the cell, signified a paradigm shift in molecular biology. Ribozymes and RNA Catalysis takes the reader through the origins of catalysis in RNA and necessarily includes significant discussion of structure and folding. The main focus of the book concerns chemical mechanism with extensive comment on how, despite the importance of RNA catalysis in the cell, its origins are still poorly understood and often controversial. The reader is given an outline of the important role of RNA catalysis in many aspects of cell function, including RNA processing and translation. There has been a significant coming together in the field of RNA in recent years and this book offers a compelling review of the whole field to date. Written by leading experts in their field, who in turn review the structural and mechanistic data for all known ribozymes this book is well suited for undergraduates, postgraduates and researchers in catalytic chemistry as well as those in related fields who require a unique overview of the subject.
Download or read book Synthesis and Purification of Small Catalytic RNA s written by Hongzhi Wang and published by . This book was released on 1993 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Spontaneous Cooperative Assembly of Replicative Catalytic RNA Systems written by Nilesh Vaidya and published by . This book was released on 2012 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt: The RNA World hypothesis proposes a period of time during the origins of life in which RNA molecules were the only source of both genotypes and phenotypes. Although a vast amount of evidence has been obtained in support of this hypothesis, a few critical demonstrations are lacking. A most crucial one is a demonstration of self-replication of RNA molecule from prebiotic soup. Previously in the Lehman laboratory, it has been demonstrated that a 198-nucleotide molecule derived from the Azoarcus group I intron can self-assemble from up to four fragments of RNA via recombination. Furthermore, the covalent full-length molecules are catalytically active and can make copies of themselves from the remaining pieces in the solution leading to their autocatalytic growth. I was able to demonstrate how this recombination system can overcome different obstacles and evolve to be an efficient replicating system. I discovered the ability of a single RNA fragment to be multifunctional in a single reaction pathway during RNA recombination events that avoids the necessity of multiple genotypes. I also confirmed the capacity of self-replicating ribozymes to form cooperative catalytic cycles and networks that would potentially prevent informational decay. Finally, I have discovered a recycling phenomenon in the RNA recombination system that exploits dynamic covalent chemistry. Recycling provides the earliest replicating system with adequate concentrations of reagents and ability to explore sequence space. Together these findings have improved our understanding of RNA recombination and bolstered the plausibility of the RNA World.
Download or read book RNA written by Marlene Belfort and published by Elsevier Publishing Company. This book was released on 1989 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: The conference on RNA: Catalysis, Splicing, Evolution brought together a unique assembly of scientific leaders in this currently very important field. Two participants of this Conference, Drs. T.R. Cech and S. Altman, were just awarded the 1989 Nobel prize in chemistry, which attests to the timeliness of this volume. The critical reviews authored by these two prominent scientists are included. For those scientists with roots in this field, who do not subscribe to GENE, this is an excellent opportunity to obtain a masterly collection of papers. All the original papers and reviews have undergone the rigorous peer review of the journal Gene.
Download or read book Ribonuclease P written by Fenyong Liu and published by Springer Science & Business Media. This book was released on 2009-12-03 with total page 293 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Discovery of Ribonuclease P and Enzymatic Activity of Its RNA Subunit Sydney Brenner and Francis H. C. Crick had a specific project in mind when they offered Sidney Altman a position in their group in 1969 to conduct postdoctoral research at the Medical Research Council Laboratory of Molecular Biology (LMB) in Cambridge, England. At the time, an intense international competition was on- ing in as many as a dozen labs to determine the three-dimensional structure of tRNA. At the LMB, Aaron Klug was attacking the structure by crystallographic analysis with Brian F. C. Clark providing large amounts of purified phenylalanine tRNA. (Eventually, Aaron announced his empirically determined 3-D structure of yeast phenylalanine tRNA, a structure that is generally common to tRNAs, due in part to several conserved, novel three-way nucleotide interactions. ) Concurrently, Michael Levitt, a Ph. D. student of Francis, was visually scrutinizing the cloverleaf secondary structure of the 14 tRNA sequences known at the time. Levitt was searching for nucleotide covariation in different parts of the molecules that were conserved in the 14 sequences known at the time. He identified a possible covariation of an apparent Watson-Crick pairing type between the residues at position 15 from the 5’ end of the tRNA and residue 48. This association implied these parts of the tRNA, namely the D loop containing residue 15 and the 5’ end of the T stem-adjoining residue 48, folded on one another in a tertiary structure shared by different tRNAs.
Download or read book Ribozymes 2 Volume Set written by Sabine Müller and published by John Wiley & Sons. This book was released on 2021-10-18 with total page 81 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ribozymes Provides comprehensive coverage of a core field in the molecular biosciences, bringing together decades of knowledge from the world’s top professionals in the field Timely and unique in its breadth of content, this all-encompassing and authoritative reference on ribozymes documents the great diversity of nucleic acid-based catalysis. It integrates the knowledge gained over the past 35 years in the field and features contributions from virtually every leading expert on the subject. Ribozymes is organized into six major parts. It starts by describing general principles and strategies of nucleic acid catalysis. It then introduces naturally occurring ribozymes and includes the search for new catalytic motifs or novel genomic locations of known motifs. Next, it covers the development and design of engineered ribozymes, before moving on to DNAzymes as a close relative of ribozymes. The next part examines the use of ribozymes for medicinal and environmental diagnostics, as well as for therapeutic tools. It finishes with a look at the tools and methods in ribozyme research, including the techniques and assays for structural and functional characterization of nucleic acid catalysts. The first reference to tie together all aspects of the multi-faceted field of ribozymes Features more than 30 comprehensive chapters in two volumes Covers the chemical principles of RNA catalysis; naturally occurring ribozymes, engineered ribozymes; DNAzymes; ribozymes as tools in diagnostics and therapy, and tools and methods to study ribozymes Includes first-hand accounts of concepts, techniques, and applications by a team of top international experts from leading academic institutions Dedicates half of its content to methods and practical applications, ranging from bioanalytical tools to medical diagnostics to therapeutics Ribozymes is an unmatched resource for all biochemists, biotechnologists, molecular biologists, and bioengineers interested in the topic.
Download or read book Structural and Mechanistic Studies of an RNA Enzyme written by Christine M. Dunham and published by . This book was released on 2003 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt:
