Download or read book Capillary Electrochromatography written by Keith D. Bartle and published by Royal Society of Chemistry. This book was released on 2001 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrochromatography (CEC) is a new and exciting hybrid separation technique that seeks to exploit the combined advantages of both capillary electrophoresis (high efficiencies) and HPLC (mobile and stationary phase selectivity). It is a technique with tremendous potential, especially in the pharmaceutical and biomedical fields. This is the first book to be devoted to the topic and presents reviews by the world leaders in the field on the theory and development of the technique and current and potential future applications. "Capillary Electrochromatography" provides an excellent introduction to the field for graduates and professionals in industry and academia with an interest in separation science.[part of the RSC Chromatography Monographs series]
Download or read book Capillary Electrochromatography written by F. Svec and published by Elsevier. This book was released on 2001-03-14 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the evolution and uses for capillary electrochromatography as a new dimension to current separation science. With the emergence of this technique the selection of available separation mechanisms increases dramatically. The book also discusses the new horizons in the separation of non-polar compounds which have been opened as a result of CEC. Over ten chapters authors cover a wide variety of topics and provide the reader with necessary theoretical background, description of the instrumentation, modes of operation and methods of detection and an overview of the broad variety of applications of capillary electrochromatography. To view the full contents as a pdf, please click /inca/publications/misc/621924_contents.pdfhere.
Download or read book Capillary Electromigration Separation Methods written by Colin F. Poole and published by Elsevier. This book was released on 2018-04-13 with total page 626 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Electromigration Separation Methods is a thorough, encompassing reference that not only defines the concept of contemporary practice, but also demonstrates its implementation in laboratory science. Chapters are authored by recognized experts in the field, ensuring that the content reflects the latest developments in research. Thorough, comprehensive coverage makes this the ideal reference for project planning, and extensive selected referencing facilitates identification of key information. The book defines the concept of contemporary practice in capillary electromigration separation methods, also discussing its applications in small mass ions, stereoisomers, and proteins. Edited and authored by world-leading capillary electrophoresis experts Presents comprehensive coverage on the subject Includes extensive referencing that facilitates the identification of key research developments Provides more than 50 figures and tables that aid in the retention of key concepts
Download or read book Practical Capillary Electrophoresis written by Robert Weinberger and published by Academic Press. This book was released on 2000-02-16 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary zone electrophoresis : basic concepts -- Capillary zone electrophoresis : methods development -- Capillary zone electrophoresis : secondary equilibrium, micelles, cyclodextrins, and related reagents -- Capillary isoelectric focusing -- Size separations in capillary gels and polymer networks -- Capillary electrochromatography -- Injection -- Detection -- Putting it all together.
Download or read book Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques written by James P. Landers and published by CRC Press. This book was released on 2007-12-18 with total page 1598 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now in its third edition, this bestselling work continues to offer state-of-the-art information on the development and employment of capillary electrophoresis. With special emphasis on microseparations and microfluidics, it features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Enhanced by contributions from leading international experts, the Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition remains a seminal reference for the chemistry, biology, and engineering fields.
Download or read book Capillary Electrophoresis Methods for Pharmaceutical Analysis written by Satinder Ahuja and published by Elsevier. This book was released on 2011-08-09 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) is a powerful analytical technique that is widely used in research and development and in quality control of pharmaceuticals. Many reports of highly efficient separations and methods have been published over the past 15 years. CE offers several advantages over high-pressure or high-performance liquid chromatography (HPLC). These include simplicity, rapid analysis, automation, ruggedness, different mechanisms for selectivity, and low cost. Moreover, EC requires smaller sample size and yet offers higher efficiency and thus greater resolution power over HPLC. These characteristics are very attractive in research and development, even more so in pharmaceutical quality control (QC) and stability monitoring (SM) studies. This book will provide busy pharmaceutical scientists a complete yet concise reference guide for utilizing the versatility of CE in new drug development and quality control. - Provides current status and future developments in CE analysis of pharmaceuticals. - Explains how to develop and validate methods. - Includes major pharmaceutical applications including assays and impurity testing.
Download or read book Liquid Chromatography written by A. Rocco and published by Elsevier Inc. Chapters. This book was released on 2013-01-08 with total page 35 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrochromatography (CEC) is one of the recently developed miniaturized analytical techniques. The separation mechanism involved in the separation process is based mainly on the analytes‘ stationary–mobile phase interactions; however, in the case of charged compounds, the different electrophoretic mobilities have to be considered. The process takes place in a capillary column, containing a selected stationary phase, where the mobile phase is delivered by an electro-osmotic flow (EOF) controlled by the application of a relatively high electric field. In contrast to what happens in liquid chromatography, where the mobile phase possesses a parabolic profile, in CEC, a plug flow profile is observed that helps achieve a higher efficiency. This overview includes the illustration of basic principles of CEC, the latest achievements concerning the instrumentation used (stationary phases, columns, detectors, hyphenation), description of method optimization, selected examples, and some potential uses of CEC in separation science.
Download or read book Analysis of Pharmaceuticals by Capillary Electrophoresis written by Kevin D. Altria and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dieser erste Titel einer ganzen Serie von anwendungsbezogenen Handbüchern zur Kapillarelektrophorese beschäftigt sich mit der Analytik von pharmazeutischen Substanzen. Dabei werden verschiedene Techniken praxisnah erläutert. Jeder, der im Labor - ob wissenschaftlich oder praxisnah - mit der Analyse von oft chiralen Pharmazeutika konfrontiert ist, wird viele Hinweise und Tips für seine Arbeit finden.USP: Einzige Monographie zur Analyse von Pharmazeutika mit CE This book describes the current state of the art for the analysis of pharmaceuticals by capillary electrophoresis and contains several hundred references to specific applications and methods. The main purpose of the book is to present the application possibilities of CE an therefore tabulated application data are provided. Chapters of the book are devoted to providing details of individual application areas such as chiral analysis, determination of drug related impurities, determination of drug counter-ions, drug residue monitoring and main component assay. An introductory chapter provides theoretical background to CE an related techniques. A chapter is dedicated to capillary electrochromatography which highlights the importance this technique currently possesses. Successful regulatory acceptance of CE methods is also described. A comprehensive chapter covers method validation aspects. Other chapters include discrete areas such as the use of non-aqueous solvents, forensic applications of CE, the application of experimental designs, determination of drugs in biofluids, and the analysis of vitamins by CE.
Download or read book Separation Methods in Drug Synthesis and Purification written by Klara Valko and published by Elsevier. This book was released on 2000-10-13 with total page 613 pages. Available in PDF, EPUB and Kindle. Book excerpt: Separation Methods in Drug Synthesis and Purification
Download or read book Carbohydrate Analysis by Modern Liquid Phase Separation Techniques written by Ziad El Rassi and published by Elsevier. This book was released on 2021-08-15 with total page 956 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges. This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques. Covers the basic principles of modern liquid phase separation techniques, along with their applications Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress
Download or read book Capillary Electrochromatography and Pressurized Flow Capillary Electrochromatography written by Ira S. Krull and published by Hnb Publishing. This book was released on 2000 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Novel Concepts and Techniques in Capillary Electrochromatography written by Chadin Kulsing and published by . This book was released on 2015 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrochromatography (CEC) has been realised as a combination of highperformance liquid chromatography (HPLC) and capillary electrophoresis (CE) allowing theseparation of analytes based on their differing adsorption and migration behaviour. There is a considerable interest in developing new methodologies for CEC, which uses chromatographic beads or monoliths as stationary phases or alternatively employs open tubular (OT)capillaries relying on surface modifications. Besides the bulk flat flow profile of the mobile phase in CEC columns allowing an enhanced resolution, due to the influence of the applied electrical field there are other possibilities to provide a higher resolution with better detection.As an additional newly means for peak sharpening and sample enrichment isotachophoresis and a "sweeping mechanism" could be envisaged, which both take advantage of the inhomogeneous electric field pattern in the capillary by choosing appropriate leading and terminating background electrolytes (BGEs). Transient isotachophoresis would be anothertechnique to provide an increased plate height in the separations, using an optimized sample matrix, e.g. containing a relatively high acetonitrile content, with the same leading and terminating BGEs.In this thesis, a new method to generate a "sharpening zone", which is similar to theconcept of transient isotachophoresis, was established using an aqueous sample matrixcontaining an organic solvent. Target analytes within this zone could be concentrated whilst they migrated through the capillary. A novel technique was developed that used the sharpening zone for sweeping and concentrating several unresolved analyte peaks allowingan enhanced peak resolution. As the aim was to concentrate only some part of the analytezone containing compounds of interest, the position and width of the sharpening zone needed to be well controlled. Various techniques to tune the width and position of the sharpening zone were thus developed, involving modifying the organic modifier content in the BGE, applying pressure additionally to the electroosmotic flow (EOF) and modifying the inner surface of the capillaries. This work also critically evaluated some related existing knowledgeand theoretical approaches in CEC including the concept of tuneable system eigenpeaks (noncomigrating or ghost peaks caused by waves of an electric field in the capillary). Although peak broadening caused by eigenpeaks (which often suppresses resolution in CEC) has been well described for aqueous or pure non-aqueous BGEs, a systematic study of eigenmobility inaqueous/acetonitrile BGEs had not been reported. The experiments related to the eigenmobility led to a new approach to tune the eigenpeak position in chromatogramswhereby a selective sample peak sharpening mechanism was elaborated using the sharpeningzone. The novel approach was demonstrated for the separation of various peptides in the CE mode and for the resolution of incompletely resolved chiral amino acids in CEC.These investigations furthermore led to the development of an appropriate stationaryphase, which was produced using techniques for an in-column synthesis of a molecularimprinted polymer (MIP). Well prepared MIPs are capable of specifically enhancing theretention of target analytes, herein focusing on enantio-selective isolation of chiral molecules. Since such a MIP was rather rigid, which often caused capillary clogging when used as a conventional monolithic stationary phase, this MIP was incorporated into porous layer opentubular (PLOT) capillaries. Numerous experimental difficulties were overcome with respect to the vinylisation of long fused-silica capillaries and for the polymerisation of the MIP within using thermal- and photo-polymerisations. A method for the characterization of suchMIP-PLOT capillaries was elaborated involving the Langmuir adsorption theory. The new columns were evaluated using chromatography, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX) and CEC analysis.All experiments were designed according to the principles of Green Chemistry. Theseconcepts involved the use of small scale analysis, simulation studies reducing the number of performed experiments, systematic studies with low amounts of solvents and materials, and optimisation of synthesis methods to reduce time, energy and reagent consumption.The theories, methods and results presented in this thesis will, in the future, allowfurther targeted developments of novel analytical tools for a green, sensitive, selective and improved resolution analysis of chemical and biological compounds including chiral compounds, peptides and proteins.
Download or read book Liquid Chromatography written by Salvatore Fanali and published by Elsevier. This book was released on 2017-06-22 with total page 808 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liquid Chromatography: Fundamentals and Instrumentation, Second Edition, is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their understanding of new fundamentals and instrumentation techniques in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making
Download or read book Practical Capillary Electrophoresis written by Robert Weinberger and published by Elsevier. This book was released on 2000-04-18 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the 1980s, capillary electrophoresis (CE) joined high-performance liquid chromatography (HPLC) as the most powerful separation technique available to analytical chemists and biochemists. Published research using CE grew from 48 papers in the year of commercial introduction (1988) to 1200 in 1997. While only a dozen major pharmaceutical and biotech companies have reduced CE to routine practice, the applications market is showing real or potential growth in key areas, particularly in the DNA marketplace for genomic mapping and forensic identification. For drug development involving small molecules (including chiral separations), one CE instrument can replace 10 liquid chromatographs in terms of speed of analysis. CE also uses aqueous rather than organic solvents and is thus environmentally friendlier than HPLC. The second edition of Practical Capillary Electrophoresis has been extensively reorganized and rewritten to reflect modern usage in the field, with an emphasis on commercially available apparatus and reagents. This authoritative and very comprehensible treatment builds on the author's extensive experience as an instructor of short courses for the American Chemical Society and for industry. Illustrated with detailed diagrams of electrophoretic phenomena Offers step-by-step methods development schemes Presents techniques for developing quantitative, robust, and precise methods Includes an extensive troubleshooting guide Updates and greatly expands on the first edition-more than 50% of the text is new Written by an internationally recognized scientist who is an instructor for American Chemical Society short courses on HPCE
Download or read book Analytical Techniques in Forensic Science written by Rosalind Wolstenholme and published by John Wiley & Sons. This book was released on 2021-01-26 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: An in-depth text that explores the interface between analytical chemistry and trace evidence Analytical Techniques in Forensic Science is a comprehensive guide written in accessible terms that examines the interface between analytical chemistry and trace evidence in forensic science. With contributions from noted experts on the topic, the text features a detailed introduction analysis in forensic science and then subsequent chapters explore the laboratory techniques grouped by shared operating principles. For each technique, the authors incorporate specific theory, application to forensic analytics, interpretation, forensic specific developments, and illustrative case studies. Forensic techniques covered include UV-Vis and vibrational spectroscopy, mass spectrometry and gas and liquid chromatography. The applications reviewed include evidence types such as fibers, paint, drugs and explosives. The authors highlight data collection, subsequent analysis, what information has been obtained and what this means in the context of a case. The text shows how analytical chemistry and trace evidence can problem solve the nature of much of forensic analysis. This important text: Puts the focus on trace evidence and analytical science Contains case studies that illustrate theory in practice Includes contributions from experts on the topics of instrumentation, theory, and case examples Explores novel and future applications for analytical techniques Written for undergraduate and graduate students in forensic chemistry and forensic practitioners and researchers, Analytical Techniques in Forensic Science offers a text that bridges the gap between introductory textbooks and professional level literature.
Download or read book Capillary Electrophoresis written by Philippe Schmitt-Kopplin and published by Springer Science & Business Media. This book was released on 2008-02-25 with total page 808 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. It includes practical details which are hard to find elsewhere. The volume is intended for beginners in the field and provides an overview of the technique and a starting point for the exploration of the defined literature on different application topics.
Download or read book Handbook of Smart Materials in Analytical Chemistry written by Miguel de la Guardia and published by John Wiley & Sons. This book was released on 2019-01-24 with total page 1000 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive guide to smart materials and how they are used in sample preparation, analytical processes, and applications This comprehensive, two-volume handbook provides detailed information on the present state of new materials tailored for selective sample preparation and the legal frame and environmental side effects of the use of smart materials for sample preparation in analytical chemistry, as well as their use in the analytical processes and applications. It covers both methodological and applied analytical aspects, relating to the development and application of new materials for solid-phase extraction (SPE) and solid-phase microextraction (SPME), their use in the different steps and techniques of the analytical process, and their application in specific fields such as water, food, air, pharmaceuticals, clinical sciences and forensics. Every chapter in Handbook of Smart Materials in Analytical Chemistry is written by experts in the field to provide a comprehensive picture of the present state of this key area of analytical sciences and to summarize current applications and research literature in a critical way. Volume 1 covers New Materials for Sample Preparation and Analysis. Volume 2 handles Analytical Processes and Applications. Focuses on the development and applications of smart materials in analytical chemistry Covers both, methodological and applied analytical aspects, for the development of new materials and their use in the different steps and techniques of the analytical process and their application in specific fields Features applications in key areas including water, air, environment, pharma, food, forensic, and clinical Presents the available tools for the use of new materials suitable to aid recognition process to the sample preparation and analysis A key resource for analytical chemists, applied laboratories, and instrument companies Handbook of Smart Materials in Analytical Chemistry, 2V Set is an excellent reference book for specialists and advanced students in the areas of analytical chemistry, including both research and application environments.
