Download or read book CRISPR Cas written by Jennifer A. Doudna and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for de.

Download or read book Phage Display written by Carlos F. Barbas and published by CSHL Press. This book was released on 2001 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.

Download or read book Clinical Laboratory Manual written by St. Marys Hospital Medical Center and published by . This book was released on 1975 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Resources in Education written by and published by . This book was released on 1997 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Early Development of Xenopus Laevis written by Hazel L. Sive and published by CSHL Press. This book was released on 2000 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis,which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopusdevelopment has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopusand vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapeswhich are cross-referenced to the appropriate section of the manual.

Download or read book Guide for the Care and Use of Laboratory Animals written by National Research Council and published by National Academies Press. This book was released on 2011-01-27 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: A respected resource for decades, the Guide for the Care and Use of Laboratory Animals has been updated by a committee of experts, taking into consideration input from the scientific and laboratory animal communities and the public at large. The Guide incorporates new scientific information on common laboratory animals, including aquatic species, and includes extensive references. It is organized around major components of animal use: Key concepts of animal care and use. The Guide sets the framework for the humane care and use of laboratory animals. Animal care and use program. The Guide discusses the concept of a broad Program of Animal Care and Use, including roles and responsibilities of the Institutional Official, Attending Veterinarian and the Institutional Animal Care and Use Committee. Animal environment, husbandry, and management. A chapter on this topic is now divided into sections on terrestrial and aquatic animals and provides recommendations for housing and environment, husbandry, behavioral and population management, and more. Veterinary care. The Guide discusses veterinary care and the responsibilities of the Attending Veterinarian. It includes recommendations on animal procurement and transportation, preventive medicine (including animal biosecurity), and clinical care and management. The Guide addresses distress and pain recognition and relief, and issues surrounding euthanasia. Physical plant. The Guide identifies design issues, providing construction guidelines for functional areas; considerations such as drainage, vibration and noise control, and environmental monitoring; and specialized facilities for animal housing and research needs. The Guide for the Care and Use of Laboratory Animals provides a framework for the judgments required in the management of animal facilities. This updated and expanded resource of proven value will be important to scientists and researchers, veterinarians, animal care personnel, facilities managers, institutional administrators, policy makers involved in research issues, and animal welfare advocates.

Download or read book Fischbach s A Manual of Laboratory and Diagnostic Tests written by Frances Fischbach and published by Lippincott Williams & Wilkins. This book was released on 2021-09-01 with total page 1496 pages. Available in PDF, EPUB and Kindle. Book excerpt: Up to date and easy to navigate, Fischbach’s A Manual of Laboratory and Diagnostic Tests, 11th Edition, details an extensive array of laboratory and diagnostic tests to prepare nurses and health professionals to deliver safe, effective, informed patient care. This proven manual is organized the way nurses think — by specimen, function, and test type— and provides current, comprehensive, step-by-step guidance on correct procedures, tips for accurate interpretation, and expert information on patient preparation and aftercare.

Download or read book The Southern California Practitioner written by and published by . This book was released on 1907 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Field Guide and Laboratory Manual for Oceanography written by William J. Wallace and published by . This book was released on 1979 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book MLA Handbook written by The Modern Language Association of America and published by Modern Language Association. This book was released on 2021-04-22 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: Relied on by generations of writers, the MLA Handbook is published by the Modern Language Association and is the only official, authorized book on MLA style. The new, ninth edition builds on the MLA's unique approach to documenting sources using a template of core elements--facts, common to most sources, like author, title, and publication date--that allows writers to cite any type of work, from books, e-books, and journal articles in databases to song lyrics, online images, social media posts, dissertations, and more. With this focus on source evaluation as the cornerstone of citation, MLA style promotes the skills of information and digital literacy so crucial today. The many new and updated chapters make this edition the comprehensive, go-to resource for writers of research papers, and anyone citing sources, from business writers, technical writers, and freelance writers and editors to student writers and the teachers and librarians working with them. Intended for a variety of classroom contexts--middle school, high school, and college courses in composition, communication, literature, language arts, film, media studies, digital humanities, and related fields--the ninth edition of the MLA Handbook offers New chapters on grammar, punctuation, capitalization, spelling, numbers, italics, abbreviations, and principles of inclusive language Guidelines on setting up research papers in MLA format with updated advice on headings, lists, and title pages for group projects Revised, comprehensive, step-by-step instructions for creating a list of works cited in MLA format that are easier to learn and use than ever before A new appendix with hundreds of example works-cited-list entries by publication format, including websites, YouTube videos, interviews, and more Detailed examples of how to find publication information for a variety of sources Newly revised explanations of in-text citations, including comprehensive advice on how to cite multiple authors of a single work Detailed guidance on footnotes and endnotes Instructions on quoting, paraphrasing, summarizing, and avoiding plagiarism A sample essay in MLA format Annotated bibliography examples Numbered sections throughout for quick navigation Advanced tips for professional writers and scholars

Download or read book Food Chemistry written by Dennis D. Miller and published by John Wiley & Sons. This book was released on 2022-03-15 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: FOOD CHEMISTRY A manual designed for Food Chemistry Laboratory courses that meet Institute of Food Technologists undergraduate education standards for degrees in Food Science In the newly revised second edition of Food Chemistry: A Laboratory Manual, two professors with a combined 50 years of experience teaching food chemistry and dairy chemistry laboratory courses deliver an in-depth exploration of the fundamental chemical principles that govern the relationships between the composition of foods and food ingredients and their functional, nutritional, and sensory properties. Readers will discover practical laboratory exercises, methods, and techniques that are commonly employed in food chemistry research and food product development. Every chapter offers introductory summaries of key methodological concepts and interpretations of the results obtained from food experiments. The book provides a supplementary online Instructor’s Guide useful for adopting professors that includes a Solutions Manual and Preparation Manual for laboratory sessions. The latest edition presents additional experiments, updated background material and references, expanded end-of-chapter problem sets, expanded use of chemical structures, and: A thorough emphasis on practical food chemistry problems encountered in food processing, storage, transportation, and preparation Comprehensive explorations of complex interactions between food components beyond simply measuring concentrations Additional experiments, references, and chemical structures Numerous laboratory exercises sufficient for a one-semester course Perfect for students of food science and technology, Food Chemistry: A Laboratory Manual will also earn a place in the libraries of food chemists, food product developers, analytical chemists, lab technicians, food safety and processing professionals, and food engineers.

Download or read book Energy Research Abstracts written by and published by . This book was released on 1984 with total page 622 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Hamster written by H.I. Siegel and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: viii The final section consists of a chapter on development by Thomas Schoenfeld and Christiana Leonard and three chapters devoted to functions of individuals. These in clude biological rhythms by Lawrence Morin, energy balance by Katarina Borer, and the visual and somatosensory systems by Barbara Finlay and Claire Berian. I am extremely delighted and proud to have played a role in this work and I wish to thank each contributor, Plenum Press (especially Kirk Jensen), Jay Rosenblatt, and the Rutgers University Research Council. In addition, I extend my thanks and my love to my family-Marilyn, Matt, Paul, Scott, and my mother and father, who taught me to appreciate animals. Harold I. Siegel Newark, New Jersey Contents I. Origin 0/ the Hamster Chapter 1 History of the Capture and Domestication of the Syrian Golden Hamster (Mesocricetus auratus Waterhouse) MICHAEL R. MURPHY 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2. The Early Years: Discovery and Description. . . . . . . . . . . . . . . . . . . . . . . . 4 3. On the Road to Jerusalem: Capture and Breeding ................... 7 4. The Invasion of America: Dissemination and Proliferation . . . . . . . . . . . . 13 . 5. New Genes on the Line: A Reconnaissance Expedition to Aleppo ....... 17 6. Conclusion ................................................. 18 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 . . . . . . . . . . . . . II. Reproductive Endocrinology Chapter 2 The Estrous Cycle ROBERT D. LISK 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 . . . . . . . . . . . . 2. Sexual Maturation-The First Estrous Cycle ....................... 24 2.1. The Ovary ............................................. 26 2.2. The Pituitary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 . . . . . . . . . . 2.3. The Brain ............................................. 31 3. Follicle Maturation and Ovulation ............................... 33 3.1. Time of Ovulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 . . . . . . . . . 3.2. Ovarian Steroids and Ovulation . . . . . . . . . . . . . . . . . . . . . . . 35 . . . . . .

Download or read book RNA written by Donald Charles Rio and published by . This book was released on 2011 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: So much has been learned about RNA in the past ten years that the ability to purify, analyze, and manipulate RNA molecules is now essential in all kinds of bioscience. Initiating RNA research can be intimidating but the new book RNA: A Laboratory Manualprovides a broad range of up-to-date techniques presented in a functional framework, so that any investigator can confidently handle RNA and carry out meaningful experiments, from the most basic to the highly sophisticated. Originating in three of the field's most prominent laboratories, this manual provides the necessary background and strategies for approaching any RNA investigation, as well as detailed protocols and extensive tips and troubleshooting information. It is required reading for every research laboratory in the life sciences.

Download or read book National Library of Medicine Audiovisuals Catalog written by National Library of Medicine (U.S.) and published by . This book was released on with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Monthly Catalogue United States Public Documents written by and published by . This book was released on 1993 with total page 1194 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Research in Education written by and published by . This book was released on 1973 with total page 1272 pages. Available in PDF, EPUB and Kindle. Book excerpt: