Download or read book Biotechnology Procedures and Experiments Handbook written by S. Harisha and published by Laxmi Publications, Ltd.. This book was released on 2008-12 with total page 726 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biotechnology Is One Of The Major New Technologies Of The Twenty-First Century That Covers Multi-Disciplinary Issues, Including Recombinant DNA Techniques, Cloning, Genetics, And The Application Of Microbiology To The Production Of Goods. It Continues To Revolutionize Treatments Of Many Diseases, And It Is Used To Deal With Environmental Solutions. The Biotechnology Procedures And Experiments Handbook Provides Practicing Professionals And Biotechnology Students Over 150 Applied, Up-To-Date Laboratory Techniques And Experiments Related To Modern Topics Such As Recombinant DNA, Electrophoresis, Stem Cell Research, Genetic Engineering, Microbiology, Tissue Culture, And More. Each Lab Technique Includes 1)A Principle, 2)The Necessary Reagents, 3)A Step By Step Procedure, And 4)A Final Result. Also Included Is A Section That Shows How To Avoid Potential Pitfalls Of A Specific Experiment. The Book Is Accompanied By A CD-ROM Containing Simulations, White Papers, And Other Relevant Material To Biotechnology.
Download or read book Experimental Biotechnology written by Sunita Dutta and published by New India Publishing. This book was released on 2011-06-03 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book is subdivided into seven sections this encompass: general procedures, like methods of pipetting, solution preparation, buffers and principles of common analytical instruments essential for laboratory biotechnology experiments. The book also includes working with nucleic acid, bacteria, enzymes, proteins; cloning experiments and a few protocols on plant biotechnology. Emphasis have been given on DNA/RNA isolation from various sources, use of restriction enzymes, ligation techniques, cloning protocols, screening of transformed cells, various electrophoresis techniques, PCR protocol, etc. The appendices in the last part are included to provide information important to he study of the above-mentioned practical as a whole. The book will be useful to students belonging to Biotechnology, agriculture and allied fields. The idea behind this practical manual was thus to provide theoretical basis of the practical study items to be undertaken in the laboratory in a lucid manner.
Download or read book Laboratory Manual for Biotechnology written by Verma, Ashish S./ Das Surajit & Singh Anchal and published by S. Chand Publishing. This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratory Manual in Biotechnology Students
Download or read book Methods in Biotechnology written by Seung-Beom Hong and published by John Wiley & Sons. This book was released on 2016-08-01 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: As rapid advances in biotechnology occur, there is a need for a pedagogical tool to aid current students and laboratory professionals in biotechnological methods; Methods in Biotechnology is an invaluable resource for those students and professionals. Methods in Biotechnology engages the reader by implementing an active learning approach, provided advanced study questions, as well as pre- and post-lab questions for each lab protocol. These self-directed study sections encourage the reader to not just perform experiments but to engage with the material on a higher level, utilizing critical thinking and troubleshooting skills. This text is broken into three sections based on level – Methods in Biotechnology, Advanced Methods in Biotechnology I, and Advanced Methods in Biotechnology II. Each section contains 14-22 lab exercises, with instructor notes in appendices as well as an answer guide as a part of the book companion site. This text will be an excellent resource for both students and laboratory professionals in the biotechnology field.
Download or read book Practical Biotechnology written by H. N. Thatoi and published by . This book was released on 2017-09-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduces the different tools and methods of molecular biology from both a theoretical and practical perspective. Discusses the principles and procedures, their potential and drawbacks, involved in experiments in laboratories. Provides information on safety guidelines, ethical issues, genetic engineering work and laboratory set-ups. The books is aimed at advanced students, as well as research scientists and technicians.
Download or read book Plant Biotechnology written by C. C. Giri and published by I. K. International Pvt Ltd. This book was released on 2013-12-30 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Biotechnology: Practical Manual covers most of the important areas of present-day plant biotechnology, beginning from plant tissue culture media preparation to transgenic plant production and related molecular biology protocols. It is meant for both students who are being introduced to plant biotechnology and those wanting to do advance research in this field. It would also be helpful for teachers in formulating their own practical protocols using different model plant systems. This book includes the principles, theoretical background and the basis for each protocol supported by the authors own research findings. This approach has been adopted to help the learners and researchers modify their procedures to develop their own protocols and methods utilizing the proven protocols included in the book.
Download or read book Laboratory Manual for Biotechnology and Laboratory Science written by Lisa A. Seidman and published by CRC Press. This book was released on 2022-12-23 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides the basic laboratory skills and knowledge to pursue a career in biotechnology. Written by four biotechnology instructors with over 20 years of teaching experience, it incorporates instruction, exercises, and laboratory activities that the authors have been using and perfecting for years. These exercises and activities help students understand the fundamentals of working in a biotechnology laboratory. Building skills through an organized and systematic presentation of materials, procedures, and tasks, the manual explores overarching themes that relate to all biotechnology workplaces including forensic, clinical, quality control, environmental, and other testing laboratories. Features: • Provides clear instructions and step-by-step exercises to make learning the material easier for students. • Emphasizes fundamental laboratory skills that prepare students for the industry. • Builds students’ skills through an organized and systematic presentation of materials, procedures, and tasks. • Updates reflect recent innovations and regulatory requirements to ensure students stay up to date. • Supplies skills suitable for careers in forensic, clinical, quality control, environmental, and other testing laboratories.
Download or read book Methods In Biotechnology written by Michael Schweizer and published by CRC Press. This book was released on 2003-09-02 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides a grounding in the experimental techniques applicable to the discipline of biotechnology. The introductory section in the text describes procedures for analysis of inorganic and organic materials, strain maintenance and fundamental experiments in gene manipulation. Other chapters deal with fermentation techniques, purification methods for substances of interest, preparation of microbial sensors and the demonstration of oil degradation by bacteria. The final chapter deals with statistical planning of experiments and scale-up methods.
Download or read book Biotechnology written by Jeffery M. Becker and published by Academic Press. This book was released on 2012-12-02 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biotechnology: A Laboratory Course is a series of laboratory exercises demonstrating the in-depth experience and understanding of selected methods, techniques, and instrumentation used in biotechnology. This manual is an outgrowth of an introductory laboratory course for senior undergraduate and first year graduate students in the biological sciences at The University of Tennessee. This book is composed of 19 chapters and begins with some introductory notes on record keeping and safety rules. The first exercises include pH measurement, the use of micropipettors and spectrophotometers, the concept of aseptic technique, and preparation of culture media. The subsequent exercises involve the application of the growth curve, the isolation, purification, and concentration of plasmid DNA from Escherichia coli, and the process of agarose gel electrophoresis. Other exercises include the preparation, purification, and hybridization of probe, the transformation of Saccharomyces cerevisiae, the transformation of E. coli by plasmid DNA, and the principles and applications of protein assays. The final exercises explore the ?-galactosidase assay and the purification and determination of ?-galactosidase in permeabilized yeast cells. This book is of great value to undergraduate biotechnology and molecular biology students.
Download or read book Molecular Biology and Biotechnology written by M. P. Bansal and published by The Energy and Resources Institute (TERI). This book was released on 2013-01-01 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biology and Biotechnology: basic experimental protocols is a compilation of methods and techniques commonly used in biomedical and biotechnological studies. The book aims to provide ample support to both students and faculty while conducting practical lessons. Four sections are covered in this book—Genomics, Proteomics, Quantitative Biochemistry, and Bioinformatics. A concise introductory note accompanies each protocol/method described for better comprehension. Every topic discussed is supported by actual methods and their expected results, and is accompanied by relevant questions.
Download or read book Experimental Procedures in Life Sciences written by S. Rajan and published by CBS Publishers & Distributors Pvt Limited, India. This book was released on 2018-04-30 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a manual for all life science students studying courses in biochemistry, biotechnology, botany, genetics, microbiology, molecular biology, zoology, nursing, and medicine, based on the author's decades-long experience in the field experiments of life sciences teaching and research.
Download or read book Fundamental Laboratory Approaches for Biochemistry and Biotechnology written by Alexander J. Ninfa and published by John Wiley & Sons. This book was released on 2009-05-26 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ninfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills in students, allowing them to learn techniques and develop the organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques with a few molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors' three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual's experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a "cookbook" approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.
Download or read book Genetic Engineering written by Hala M. Abdelmigid and published by CreateSpace. This book was released on 2013-11 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt: The primary motive for compiling and publishing this manual was to provide scientists, researchers, and students from national agricultural research systems, universities, and small private companies in developing countries, as well as advanced research institutions in the developed world, with a useful guide on the protocols currently in use in genetic engineering. This manual is intended to introduce you to some of the most widely used experimental procedures in biotechnology, including DNA isolation, manipulation, and cloning. You will also gain some familiarity with some of the types of equipment frequently used in biochemistry and molecular biology.The objective of this laboratory course is to provide you with hands-on experience in some of the basic, but essential laboratory skills required in molecular biology and biotechnology. Emphasis will be placed on understanding the concepts behind designing and implementing controlled experiments. The genetic engineering laboratory, like all laboratory courses, is an exploration of procedures. This means that, in order to get full benefit from the course, you will need to read the Manual; this reading will provide background information and an outline of the procedures to be performed. If you do not do this, you will find yourself wasting large amounts of class time, and annoying both your lab partners and your instructor. To encourage your understanding of the material, you will have problem sets that cover material related to the planned experiments. The genetic engineering laboratory is conducted as a “directed” research project. This means that although the general procedures are well established, the overall goal of each experiment is the acquisition of new information. Because of the nature of scientific research, predicting the outcome of experiments that have not previously been performed is difficult. It may therefore be necessary to design new experiments based on the results of previous ones, or to repeat experiments that yielded ambiguous results. On the other hand, if you approach the course will an open and flexible mindset, you will learn how research is performed in a genetic engineering laboratory.
Download or read book Molecular Biology Techniques written by Sue Carson and published by Academic Press. This book was released on 2019-03-05 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions
Download or read book Laboratory Manual Of Biochemistry written by R.S.Sengar and published by New India Publishing Agency. This book was released on 2014-01-15 with total page 11 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present book "Laboratory Manual of Biochemistry: Methods and Techniques" is the outcome of 17 years of teaching and research experience of the authors. Biochemistry is a comparatively recent branch but the utility and variability of research work and the dazzling pace of its development has positioned this discipline in the forefront of scientific hierarchy. As Biochemistry works at a molecular level (i.e. finer than that accessed by the ultra-modern optical or phase-contrast microscopes) it embraces other disciplines also. Biochemistry has thus strengthened the integrated approach concept and solving biological riddles. Biochemical Techniques are used in all branches of biological sciences and biotechnology. Biochemical experiments are conducted in the laboratory as practical as well as for persuing research. A researcher has to refer to many journals and books before he/she could get to the working protocol for his/her experiment. This book attempts to give often-used methods in a single volume. This first edition is divided into 11 Units. Each experiment includes principle, requirements, procedure, calculation and observations. At the end of each , references for additional reading are provided. Important precautions, warnings and tips are given under the notes section. In addition, there are 12 appendices, which give minute details on basic chemistry, buffer preparations and other aspects required for the conduct of the experiments. The methods given in the book will be useful for conducting practical classes at the undergraduate and postgraduate levels in biochemistry, biotechnology, microbiology, agricultural sciences, environmental science, botany, zoology, nutrition, pharmaceutical science and other biology-related subjects. This book will be a bonanza for the research workers since it covers procedures from the classical basic biochemistry to the modern PCR techniques.
Download or read book Laboratory Manual in Industrial Biotechnology written by P. Chellapandi and published by Pointer Publishers. This book was released on 2007 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: Industrial Biotechnology Can Play A Vital Role In Overcoming The Fundamental Challenges Including Employment Opportunity And Manpower Development. The Main Aim Of The Book To Review Fundamental Bio-Analytical Techniques Involved In Common Fermentation Processes And To Provide An Up-To-Date Account Of Current Knowledge In Fermentation And Biochemical Technology With Special Emphases In Microbial Systems. It Has Covered Useful Protocols For Recognizing The Fundamentals Of Fermentation Technology And For Describing Current Knowledge In Microbial Technology, Especially In Applications Of The Modern Fungal Systems In Bioprocess Developments With Industrial Practices. Procedures Are Described Step By Step For The User To Carry Out Experiments Without Further Assistance. In Each Chapter, Short Summary Of Appropriate Products Are Explained Comprehensively For Users So As To Understand The Concepts Of Fermentation And Biochemical Mechanisms Of Respective Industrial Organisms. This Lab Manual Includes 10 Major Units In Industrial Biotechnology Area, Including Animal And Agricultural Biotechnology. Each Unit Is Further Divided Into The Related Production Of Bio-Products And Frequently Associated Analytical Methods In Coincided Manner. Physiochemical And Microbiological Analysis Are Well Documented With Reagents Prepration And Media Composition. The Significance Of Using This Manual Is That There Is No Need To Use Any Sophisticated Instrument And Very Cost Effective Chemicals For Analysis. The Main Units Comprised In This Book Are, " Molecular And Microbial Techniques " Analysis Of Fermentation Substrate " Immunobiotechnology " Agricultural Biotechnology " Dairy Biotechnology " Food Biotechnology " Enzyme Biotechnology " Biochemical Technology " Pharmaceutical Biotechnology " Biogas Technology This Book Will Be Useful To Students Of Biochemical Engineering, Biotechnology, Microbiology, Fermentation Technology And Biochemistry, Who Are Interested In The Areas Of Industrial Biotechnology.
Download or read book LABORATORY HANDBOOK ON BIOCHEMISTRY written by SHANMUGAM, S. and published by PHI Learning Pvt. Ltd.. This book was released on 2019-12-01 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: This systematically designed laboratory handbook elucidates a number of techniques which help students carry out various experiments in the field of biochemistry. The experimental protocols described in this book have been standardized and performed in the authors’ own laboratory. It is basically intended for the undergraduate and postgraduate students of life sciences (biochemistry, microbiology, biotechnology, plant biotechnology, animal biotechnology, botany and zoology) and engineering (biotechnology and biomedical) for their laboratory courses. The students usually have to refer to many journals and books to find the right procedure for performing experiments, hence this handbook is an attempt to provide them with the frequently used methods in a handy format, including explanations of principles, procedures and interpretations of results of the experiments. Now, in its second edition, the book introduces ten new experiments in a step-by-step procedural format under In Vitro Enzymatic Anti-oxidant Assays explaining Determination of Nitric Oxide Radical Scavenging Activity, Determination of Catalase Activity, Determination of Laccase Activity and Concentration and Diafiltration. KEY FEATURES • Provides a general procedure of the experiments in an easy-to-understand tabulated format. • Presents the physiological importance of bio-components like amino acids, uric acid, carbohydrates, proteins, etc. in the human body as an added feature. • Gives information on preparation of laboratory reagents in separate appendices. • Provides illustrations for better understanding of the experiments. TARGET AUDIENCE • B.Sc. / M.Sc. Life sciences (Biochemistry, Microbiology, Biotechnology, Plant Biotechnology, Animal Biotechnology, Botany and Zoology) • B.Tech (Biotechnology and Biomedical Engineering)
