Download or read book Biophysical Labeling Methods in Molecular Biology written by Gertz I. Likhtenshtein and published by Cambridge University Press. This book was released on 1993-03-26 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: This 1993 volume covers all aspects of the field of physical labels - the theoretical bases, the experimental techniques - and shows how to interpret the resulting data.
Download or read book Biophysical Labeling Methods in Molecular Biology written by Gerts Ilích Likhtenshtein and published by . This book was released on 1993 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isotope Labeling of Biomolecules Applications written by and published by Academic Press. This book was released on 2016-01-11 with total page 513 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isotope Labeling of Biomolecules: Applications, the latest in the Methods in Enzymology series, focuses on stable isotope labeling methods and applications for biomolecules. This practical guide to biomolecular labeling looks at new techniques that are becoming widely used. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Focuses on stable isotope labeling of biomolecules, which is important for structural studies of proteins and nucleic acids
Download or read book Isotope Labeling of Biomolecules Labeling Methods written by and published by Academic Press. This book was released on 2015-11-26 with total page 662 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isotope Labeling of Biomolecules – Labeling Methods, the latest volume of the Methods in Enzymology series contains comprehensive information on stable isotope labeling methods and applications for biomolecules. Contains contributions from leading authorities in the field of isotope labeling of biomolecules Informs and updates on the latest developments in the field Provides comprehensive information on stable isotope labeling methods and applications for biomolecules
Download or read book Biophysical labeling met written by Likhtenshtein and published by . This book was released on 1993 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proximity Labeling written by Murat Sunbul and published by Springer Nature. This book was released on 2019-05-23 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.
Download or read book Biophysical Chemical and Functional Probes of RNA Structure Interactions and Folding Part B written by Daniel Herschlag and published by Academic Press. This book was released on 2009-11-16 with total page 567 pages. Available in PDF, EPUB and Kindle. Book excerpt: This MIE volume provides laboratory techniques that aim to predict the structure of a protein which can have tremendous implications ranging from drug design, to cellular pathways and their dynamics, to viral entry into cells. Expert researchers introduce the most advanced technologies and techniques in protein structure and folding Includes techniques on tiling assays
Download or read book Biophysical Techniques in Photosynthesis written by Thijs Aartsma and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 538 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first volume on Biophysical Techniques in Photosynthesis Research, published in 1996, new experimental techniques and methods have been devised at a rapid pace. The present book is a sequel which complements the publication of the first volume by providing a comprehensive overview of the most important new techniques developed over the past ten years, especially those that are relevant for research on the mechanism and fundamental aspects of photosynthesis.
Download or read book Biophysical and Computational Tools in Drug Discovery written by Anil Kumar Saxena and published by Springer Nature. This book was released on 2021-10-18 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews recent physicochemical and biophysical techniques applied in drug discovery research, and it outlines the latest advances in computational drug design. Divided into 10 chapters, the book discusses about the role of structural biology in drug discovery, and offers useful application cases of several biophysical and computational methods, including time-resolved fluorometry (TRF) with Förster resonance energy transfer (FRET), X-Ray crystallography, nuclear magnetic resonance spectroscopy, mass spectroscopy, generative machine learning for inverse molecular design, quantum mechanics/molecular mechanics (QM/MM,ONIOM) and quantum molecular dynamics (QMT) methods. Particular attention is given to computational search techniques applied to peptide vaccines using novel mathematical descriptors and structure and ligand-based virtual screening techniques in drug discovery research. Given its scope, the book is a valuable resource for students, researchers and professionals from pharmaceutical industry interested in drug design and discovery.
Download or read book Stem Cell Labeling for Delivery and Tracking Using Noninvasive Imaging written by Dara L. Kraitchman and published by CRC Press. This book was released on 2011-11-18 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem Cell Labeling for Delivery and Tracking Using Noninvasive Imaging provides a comprehensive overview of cell therapy imaging, ranging from the basic biology of cell therapeutic choices to the preclinical and clinical applications of cell therapy. It emphasizes the use of medical imaging for therapeutic delivery/targeting, cell tracking, and det
Download or read book Biophysical Tools for Biologists written by John J. Correia and published by Academic Press. This book was released on 2011-09-21 with total page 996 pages. Available in PDF, EPUB and Kindle. Book excerpt: Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vitro and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval. Describes techniques in the context of important biological problems Delineates critical steps and potential pitfalls for each method Includes full-color plates to illustrate techniques
Download or read book Phosphate Labeling and Sensing in Chemical Biology written by Henning Jacob Jessen and published by Springer. This book was released on 2017-07-08 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: The series Topics in Current Chemistry Collections presents critical reviews from the journal Topics in Current Chemistry organized in topical volumes. The scope of coverage is all areas of chemical science including the interfaces with related disciplines such as biology, medicine and materials science. The goal of each thematic volume is to give the non-specialist reader, whether in academia or industry, a comprehensive insight into an area where new research is emerging which is of interest to a larger scientific audience. Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field.
Download or read book Comprehensive Biophysics written by and published by Academic Press. This book was released on 2012-04-12 with total page 3533 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biophysics is a rapidly-evolving interdisciplinary science that applies theories and methods of the physical sciences to questions of biology. Biophysics encompasses many disciplines, including physics, chemistry, mathematics, biology, biochemistry, medicine, pharmacology, physiology, and neuroscience, and it is essential that scientists working in these varied fields are able to understand each other's research. Comprehensive Biophysics, Nine Volume Set will help bridge that communication gap. Written by a team of researchers at the forefront of their respective fields, under the guidance of Chief Editor Edward Egelman, Comprehensive Biophysics, Nine Volume Set provides definitive introductions to a broad array of topics, uniting different areas of biophysics research - from the physical techniques for studying macromolecular structure to protein folding, muscle and molecular motors, cell biophysics, bioenergetics and more. The result is this comprehensive scientific resource - a valuable tool both for helping researchers come to grips quickly with material from related biophysics fields outside their areas of expertise, and for reinforcing their existing knowledge. Biophysical research today encompasses many areas of biology. These studies do not necessarily share a unique identifying factor. This work unites the different areas of research and allows users, regardless of their background, to navigate through the most essential concepts with ease, saving them time and vastly improving their understanding The field of biophysics counts several journals that are directly and indirectly concerned with the field. There is no reference work that encompasses the entire field and unites the different areas of research through deep foundational reviews. Comprehensive Biophysics fills this vacuum, being a definitive work on biophysics. It will help users apply context to the diverse journal literature offering, and aid them in identifying areas for further research Chief Editor Edward Egelman (E-I-C, Biophysical Journal) has assembled an impressive, world-class team of Volume Editors and Contributing Authors. Each chapter has been painstakingly reviewed and checked for consistent high quality. The result is an authoritative overview which ties the literature together and provides the user with a reliable background information and citation resource
Download or read book ESR Spectroscopy in Membrane Biophysics written by Marcus A. Hemminga and published by Springer Science & Business Media. This book was released on 2007-08-14 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Starting from a comprehensive quantum mechanical description, this book introduces the optical (IR, Raman, UV/Vis, CD, fluorescence and laser spectroscopy) and magnetic resonance (1D and 2D-NMR, ESR) techniques. The book offers a timely review of the increasing interest in using spin-label ESR as an alternative structural technique for NMR or X-ray diffraction. Future aspects are treated as well, but only as an illustration of the progress of ESR in this field.
Download or read book Site Specific Protein Labeling written by Arnaud Gautier and published by Humana Press. This book was released on 2015-01-06 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Chapters provide protocols for labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols provides a comprehensive overview on the most relevant and established labeling methodologies, and helps researchers to choose the most appropriate labeling method for their biological question.
Download or read book Spin Labeling written by Lawrence J. Berliner and published by Springer Science & Business Media. This book was released on 2006-01-14 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: We present here the second issue devoted entirely to the spin-labeling technique as part of Biological Magnetic Resonance. Volume 14 commemorates a modifi- tion in our editorial policy with the retirement of my esteemed coeditor, Jacques Reuben. From thisjuncture into the future, each issue will focus on some special topic in magnetic resonance. Each volume will be organized in most cases by guest editors, for example forthcoming issues will address the following topics: in vivo magnetic resonance (P. Robitaille and L. J. Berliner, eds. ) Modern techniques in proton NMR ofproteins (R. Krishna and L. J. Berliner, eds. ) Instrumental techniques of EPR (C. Bender and L. J. Berliner, eds. ) Thecurrent volume, Spin Labeling: The NextMillennium, presents an excellent collection of techniques and applications that evolved during the past decade since the last volume, volume 8 (1989). Someobvious omissions, such as multiquantum EPR and very high-frequency FT-ESR were unfortunately not possible for this volume. Perhaps they will appear in Spin Labeling: 2001. Lastly it is a pleasure to honor two scientists whose contributions were both pioneering and pivotal to the spin label technique: Professor Eduard G. Rozantsev (Moscow), whose synthetic feats in nitroxyl chemistry set the broad stage for a versatile catalog of labels; and Professor Harden M. McConnell, last year's Int- national ESR (EPR) Society Gold Medalist, who conceived and developed the spin label technique to address many biological problems (proteins, enzymes, m- branes, cells, immune response, etc. ). Lawrence J.
Download or read book Spin Labeling written by Lawrence J. Berliner and published by Academic Press. This book was released on 2013-10-22 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spin Labeling: Theory and Applications covers the background, theory, and applications of spin labeling. The book starts by providing an introduction about electron spin resonance in biology and a reporter group technique of spin labelling. The text then describes the principles and theories of magnetic resonance; the theory of slow tumbling ESR spectra for nitroxides; and the influence of electron-electron interactions on the appearance of the electron resonance spectrum. The chemistry of spin labels; the molecular structures of nitroxides; the instrumental aspects of spin labeling; as well as the use of spin labels for studying the structure and function of enzymes are also considered. The book further discusses spin-label-induced nuclear magnetic resonance relaxation studies of enzymes; anisotropic motion in liquid crystalline structures; and the use of oriented lipid systems as model membranes. The text also looks into the application of lipid spin labels in biological membranes as well as the molecular motion in biological membranes. Chemists, molecular biologists, chemical physicists, people involved in the study of physical spectrometry, and graduate students taking related courses will find the book invaluable.
