Download or read book Biomedical Research Applications of Scanning Electron Microscopy written by Katharine Elizabeth Carr and published by . This book was released on 1983 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomedical Research Applications of Scanning Electron Microscopy written by Gisele M. Hodges and published by . This book was released on 1980 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Techniques In Microscopy For Biomedical Applications written by Terje Dokland and published by World Scientific Publishing Company. This book was released on 2006-09-28 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second volume of the series Manuals in Biomedical Research, this book is aimed to be both a concise introduction to the diverse field of microscopy and a practical guide those who require the use of microscopic for methods in their research. It provides young as well as experienced scientists a state-of-the-art multidisciplinary overview of microscopic techniques, covering all the major microscopy fields in biomedical sciences and showing their application in evaluating samples ranging from molecules to cells and tissues.Microscopy has revolutionized our understanding of biological events. Within the last two decades, microscopic techniques have provided insights into the dynamics of biological processes that regulate such events. Biological discovery, to a large extent, depends on advances in imaging techniques and various microscopic techniques have emerged as central and indispensable tools in the biomedical sciences.The four authors bring with them extensive experiences spanning across disciplines such as Microbiology, Molecular and Cell Biology, Tissue Engineering, Biomedical and Regenerative Medicine and so forth, reinforcing the fact that microscopy has proven useful in countless investigations into the mysteries of life.
Download or read book Atomic Force Microscopy written by Pier Carlo Braga and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: The natural, biological, medical, and related sciences would not be what they are today without the microscope. After the introduction of the optical microscope, a second breakthrough in morphostructural surface analysis occurred in the 1940s with the development of the scanning electron microscope (SEM), which, instead of light (i. e. , photons) and glass lenses, uses electrons and electromagnetic lenses (magnetic coils). Optical and scanning (or transmission) electron microscopes are called “far-field microscopes” because of the long distance between the sample and the point at which the image is obtained in comparison with the wavelengths of the photons or electrons involved. In this case, the image is a diffraction pattern and its resolution is wavelength limited. In 1986, a completely new type of microscopy was proposed, which, without the use of lenses, photons, or electrons, directly explores the sample surface by means of mechanical scanning, thus opening up unexpected possibilities for the morphostructural and mechanical analysis of biological specimens. These new scanning probe microscopes are based on the concept of near-field microscopy, which overcomes the problem of the limited diffraction-related resolution inherent in conventional microscopes. Located in the immediate vicinity of the sample itself (usually within a few nanometers), the probe records the intensity, rather than the interference signal, thus significantly improving resolution. Since the most we- known microscopes of this type operate using atomic forces, they are frequently referred to as atomic force microscopes (AFMs).
Download or read book Biomedical Applications of Microprobe Analysis written by Peter Ingram and published by Academic Press. This book was released on 1999-10-29 with total page 573 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical Applications of Microprobe Analysis is a combination reference/laboratory manual for the use of microprobe analysis in both clinical diagnostic and research settings. Also called microchemical microscopy, microprobe analysis uses high-energy bombardment of cells and tissue, in combination with high resolution EM or confocal microscopy to provide a profile of the ion, metal, and mineral concentrations present in a sample. This allows insight into the physiology and pathophysiology of a wide variety of cells and tissues.This book describes methods for obtaining detailed information about the identity and composition of particles too small to be seen with the naked eye and describes how this information can be useful in diagnostic and biomedical research. Up-to-date review of electron microprobe analysis Detailed descriptions of sample preparation techniques Recent technologies including confocal microscopy, infrared microspectroscopy, and laser raman spectroscopy Over 100 illustrations with numerous specific applications Contributions by world-renowned experts in the field Brief summary of highlights precedes each chapter
Download or read book Methods of Preparation for Electron Microscopy written by David G. Robinson and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.
Download or read book Scanning Electron Microscopy for the Life Sciences written by Heide Schatten and published by Cambridge University Press. This book was released on 2013 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.
Download or read book Application of Electron Microscopy to Biomedical Research written by David James Paton Ferguson and published by . This book was released on 1994 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Scanning Electron Microscopy written by and published by . This book was released on 1973 with total page 1064 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Scanning Electron Microscopy in BIOLOGY written by R.G. Kessel and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the continuing quest to explore structure and to relate struc tural organization to functional significance, the scientist has developed a vast array of microscopes. The scanning electron microscope (SEM) represents a recent and important advance in the development of useful tools for investigating the structural organization of matter. Recent progress in both technology and methodology has resulted in numerous biological publications in which the SEM has been utilized exclusively or in connection with other types of microscopes to reveal surface as well as intracellular details in plant and animal tissues and organs. Because of the resolution and depth of focus presented in the SEM photograph when compared, for example, with that in the light microscope photographs, images recorded with the SEM have widely circulated in newspapers, periodicals and scientific journals in recent times. Considering the utility and present status of scanning electron microscopy, it seemed to us to be a particularly appropriate time to assemble a text-atlas dealing with biological applications of scanning electron microscopy so that such information might be presented to the student and to others not yet familiar with its capabilities in teaching and research. The major goal of this book, therefore, has been to assemble material that would be useful to those students beginning their study of botany or zoo logy, as well as to beginning medical students and students in advanced biology courses.
Download or read book Scanning Electron Microscopy written by IIT Research Institute and published by . This book was released on 1974 with total page 1064 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Advances in Biomedical Applications of the Scanning Electron Microscope written by Illinois Institute of Technology. Research Institute and published by . This book was released on 1974* with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Advanced Techniques in Biological Electron Microscopy written by J.K. Koehler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.
Download or read book Electron Microscopy Characterization of Nanoparticles for Biomedical Application written by Yitian Zeng and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past two decades, nanotechnology has demonstrated great potential in the field of biology and medicine. Nanomaterials, such as gold nanoparticles, with their superior chemical and physical properties, are widely used in a variety of biomedical research, ways ranging from cancer early detection (e.g. liquid biopsy) to treatment (e.g. hyperthermia therapy). On the other hand, advances in nano characterization techniques have enabled new investigations of naturally occurring nanoscale features in the body, in order to understand the pathological processes associated with them. This dissertation describes the use of advanced electron microscopy to characterize nanomaterials of relevance to the field of medicine. Some nanoparticles are lithographically fabricated, some are chemically synthesized, and others are directly extracted from tissues and cells. The morphological, crystallographic, chemical, optical and other physical properties of these nanoparticles are evaluated using a combination of imaging, diffraction and advanced spectroscopy techniques in a transmission electron microscope (TEM) and scanning electron microscope (SEM). In the first part of this work, surface enhanced Raman scattering (SERS) gold nanoparticles were optimized for sensitive detection of tumors by correlating localized surface plasmon resonances (LSPR) with surface enhancement. Electron beam lithography was used to prototype gold nanostructures with a wide variety of shapes, size, interspacing and in different dielectric environments. The LSPR of these structures were measured using electron energy loss spectroscopy (EELS) in a transmission electron microscope operated in scanning mode (STEM) with monochromation. It is found that nanoparticle size and dielectric environment have the most significant effects on localized surface plasmons, which is collective oscillation modes of the free electron gas at the metal surface. By contrast, interspacing has a weaker influence on surface plasmons for the range studied in this dissertation. Larger nanoparticle size and higher dielectric constant result in lower surface plasmon energies. The novelty of this work is that the LSPR from various nanostructure arrays were correlated with their Raman spectra acquired at different illuminating laser energies after incubation with a Raman dye. It is demonstrated that the largest Raman signal intensities are obtained when the illuminating laser energy coincides with, or is slightly higher than, the gold nanoparticle surface plasmon resonance energies (e.g. 90 nm diameter nanodisc particles with a LSPR energy of 1.94 eV show strongest Raman signal enhancement under a 638 nm (1.94 eV) wavelength laser excitation). By comparing various nanostructure shapes with similar surface plasmon energies, it is shown that sharper nanostructures tend to exhibit stronger surface enhancement. This information is useful in designing nanoparticle combinations to generate the largest SERS enhancement for detection of early stage medical problems such as cancer. The second part of this work is focused on naturally occurring particles, in particular, iron deposits in the hippocampal region of a brain to understand the pathological processes related to Alzheimer's diseases (AD). Recent work on iron accumulation in AD brains has led researchers to hypothesize that the oxidation state of iron may be related to neurodegeneration because ferrous iron, compared with ferric iron, may cause oxidative damage and antioxidant depletion on neurons. First, iron rich regions from AD brain tissues were located using correlative magnetic resonance imaging (MRI), optical microscopy (OM), SEM and energy dispersive spectroscopy (EDS). Cross-sections of tissue containing iron deposits were then extracted using focused ion beam (FIB) and subsequently thinned to make them electron transparent. The relative concentrations of ferric and ferrous ions within the iron deposits were determined by studying the intensity ratios of Fe L3:L2 edges from the energy loss near edge structure (ELNES) of the Fe L edge using monochromated STEM-EELS as above. Massive correlation across biological and physical microscopy and spectroscopy techniques was demonstrated for the first time in this work. These observations and insights provide supporting evidence of ferrous iron as being possibly associated with AD. The third and final section addresses characterization of artificial and natural nanoparticle composites. These hybrid nanoparticles, fabricated via a simple extrusion method, can greatly increase the target specificity and cellular uptake in various biomedical applications such as cancer imaging and drug delivery. A negative staining technique was utilized to provide contrast of biological components of these nanoparticles in TEM, and specific proteins of interest were labeled with antibodies conjugated to 100 nm diameter gold iron oxide nanoparticles (GIONs). The combination of superior magnetic, photonic and other physical properties from artificial nanoparticles, along with cellular specificity and biological compatibility from natural nanoparticles makes these hybrid nanoparticles useful for multi-modality imaging and possible medical treatment. Overall, electron microscopy is a versatile and powerful methodology for characterization of a wide variety of nanomaterials. Advanced microscopic and spectroscopic techniques such as monochromatic STEM-EELS and EDS, which are rarely used in the life sciences, have great potential in bringing unique insight into biomedical research.
Download or read book Biomedical Research Applications of Scanning Electron Microscopy written by Gisele M. Hodges and published by . This book was released on 1979 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book ADVANCES IN BIOMEDICAL APPLICATIONS OF THE SCANNING ELECTRON MICROSCOPE PROCEEDINGS OF A WORKSHOP written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Advertiser s Brochure and Exhibition Information written by Illinois Institute of Technology Research Institute and published by . This book was released on 1974 with total page 68 pages. Available in PDF, EPUB and Kindle. Book excerpt:
