Download or read book Basics of CRISPR Cas Mediated Plant Genome Editing written by Anshu Alok and published by CRC Press. This book was released on 2024-08-23 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: CRISPR/Cas has emerged, as a powerful genetic engineering tool. It is simple yet highly affordable and robust. This book provides a basic introduction to the mechanism and architecture of the CRISPR/Cas. It enlightens the readers about the new advancements such as gene insertion, transcriptional activation, suppression, gene tagging, multiplexing, base editing, prime editing, and tissue culture free editing which makes it easier for crop improvement. With such technologies, application of genome editing has opened new paths for crop improvements and sustainable agriculture. The current book will be useful for beginners to understand these tools and how to use them in a better and more efficient way.
Download or read book CRISPR Cas Mediated Genome Editing in Plants written by Deepu Pandita and published by CRC Press. This book was released on 2023-06-30 with total page 463 pages. Available in PDF, EPUB and Kindle. Book excerpt: With over 820 million people facing hunger in today’s world, the need of the hour is the design of plant varieties with high yield, improved traits, and resistance properties in order to mitigate the detrimental impacts of biotic and abiotic stress conditions on food crops. This volume highlights the use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated nuclease proteins as a solution for sustainable agriculture. CRISPR/Cas-mediated genome editing (GE) provides a robust, simple, precise, efficient, economical, and revolutionary toolbox that leads to improved plant traits with enhanced yield, quality, and resistance against various stresses, including climate-related stress, insect pests and diseases, pollution-related stresses, etc. Key features of the book: Explains the differences between conventional breeding, genetically modified crops, and genome editing approaches using CRISPR/Cas Introduces the tools of genome editing, such as ZFNS (zinc finger nucleases), mega nucleases, and TLENS (transcription activator-like effector nucleases) Covers the history, origin, discovery, structure, and classification of CRISPR/Cas Examines the databases and computational approaches of CRISPR/Cas Highlights genome editing of important crop plants with CRISPR/Cas systems, including wheat, maize. tomato, Brassica crops, rice, fruits Explores potential applications of CRISPR/Cas systems for climate smart crops
Download or read book Gene Editing in Plants written by Ashwani Kumar and published by Springer Nature. This book was released on with total page 871 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Genome Editing with CRISPR Systems written by Yiping Qi and published by Springer Nature. This book was released on 2019-01-04 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.
Download or read book Genome Editing in Plants written by Om Prakash Gupta and published by CRC Press. This book was released on 2021-07-25 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genome Editing in Plants: Principles and Applications addresses the information of genome editing starting from principles and historical aspects to the latest advancements in the field. As genome-editing technology has emerged as promising and cutting edge, researchers around the world have started producing original research outputs, which have significantly improved our current understanding and potential of this technology. The initial chapters of this book describe different genome-editing tools as well as their principles and applications. Other chapters are dedicated to the present status and future applications of genome-editing techniques in various crop improvement programmes. Some of the advanced applications of CRISPR/Cas tools, such as base editing and RNA detection, along with regulatory aspects of genome-edited crops are described in detail. This book serves as a valuable resource to researchers in the field of crop improvement; graduate and postgraduate students engaged in plant molecular biology and biotechnology; academicians; and policy makers. Key Features: Addresses topics associated with historical development and principles of genome-editing technology Addresses basic mechanisms operating under each genome-editing technology Addresses its application in plants to design crops as per the current and future demands Addresses the regulatory mechanisms of genome-edited crops
Download or read book Genome Engineering via CRISPR Cas9 System written by Vijai Singh and published by Academic Press. This book was released on 2020-02-21 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields.
Download or read book Genome Editing written by Shabir Hussain Wani and published by Springer Nature. This book was released on 2022-11-08 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms. Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement. Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Download or read book Plant Genome Editing Policies and Governance written by Thorben Sprink and published by Frontiers Media SA. This book was released on 2020-04-22 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book CRISPR Cas Genome Editing written by Anjanabha Bhattacharya and published by Springer Nature. This book was released on 2020-12-11 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers a comprehensive collection of papers on CRISPR/Cas genome editing in connection with agriculture, climate-smart crops, food security, translational research applications, bioinformatics analysis, practical applications in cereals, floriculture crops, engineering plants for abiotic stress resistance, the intellectual landscape, regulatory framework, and policy decisions. Gathering contributions by internationally respected experts in the field of CRISPR/Cas genome editing, the book offers an essential guide for researchers, students, teachers and scientists in academia; policymakers; and public companies, private companies and cooperatives interested in understanding and/or applying CRISPR/Cas genome editing to develop new agricultural products.
Download or read book A Roadmap for Plant Genome Editing written by Agnès Ricroch and published by Springer Nature. This book was released on 2024-01-15 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book is an update of genome editing techniques applied to a range of plants. We discuss the latest techniques and applications to cereals, roots and tubers, oilseed crops, fruit and forest trees, vegetables, legumes and algae including resistance to biotic and abiotic stresses, improved quality, drug production, yield and adaptation to climate change. The regulations in different countries worldwide, the patentibility and the perception by society of the applications of new genomic techniques are examined. This book is written by a multidisciplinary and multisectoral collective of high-profile scientists and other experts belonging to the COST Action network PlantEd, which is mainly European but with contributions from American, Australian, Canadian, Chinese, Indian, Iranian, Pakistani and Peruvian scientists. The book is aimed at a wide audience consisting of students, academics, private and public breeders, other actors in the food and bioeconomy value chains and policy and law makers. This is an open access book.
Download or read book Gene Editing in Plants written by and published by Academic Press. This book was released on 2017-08-31 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene Editing in Plants, Volume 149 aims to provide the reader with an up-to-date survey of cutting-edge research with gene editing tools and an overview of the implications of this research on the nutritional quality of fruits, vegetables and grains. New chapters in the updated volume include topics relating to Genome Engineering and Agriculture: Opportunities and Challenges, the Use of CRISPR/Cas9 for Crop Improvement in Maize and Soybean, the Use of Zinc-Finger Nucleases for Crop Improvement, Gene Editing in Polyploid Crops: Wheat, Camelina, Canola, Potato, Cotton, Peanut, Sugar Cane, and Citrus, and Gene Editing With TALEN and CRISPR/Cas in Rice. This ongoing serial contain contributions from leading scientists and researchers in the field of gene editing in plants who describe the results of their own research in this rapidly expanding area of science. Shows the importance of revolutionary gene editing technology on plant biology research and its application to agricultural production Provides insight into what may lie ahead in this rapidly expanding area of plant research and development Contains contributions from major leaders in the field of plant gene editing
Download or read book CRISPR Crops written by Aftab Ahmad and published by Springer Nature. This book was released on 2021-01-08 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book compiles the latest applications of the cutting-edge gene editing tool CRISPR/Cas in the area of crop improvement. It begins with an introduction to the technique and its application in crop plants. Next, it gives an updated overview of available delivery methods, design tools and resources in CRISPR/Cas. The book subsequently reviews the applications of CRISPR/Cas in connection with e.g. insect stress, disease stress, abiotic stress, nutritional and yield improvement in crop plants, etc. It also discusses the various regulatory, ethical and social aspects of the technique that must be kept in mind when designing experiments. In closing, the book summarizes the status quo and outlines future prospects for the tool in crop improvement and food security. Given its scope, the book will especially benefit students and researchers in food science, biotechnology, agriculture and the plant sciences.
Download or read book The CRISPR Cas Tool Kit for Genome Editing written by Aftab Ahmad and published by Springer Nature. This book was released on 2022-01-01 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses CRISPR/Cas- one of the most powerful tools available to scientists for genome editing. CRISPR/Cas is not only a genome editing tool, but researchers have also engineered it for gene regulation, genome imaging, base editing and epigenome regulations. This book describes the entire toolkit for CRISPR/Cas. The opening section gives an introduction to the technique and compares it with other genome editing tools. Further section gives a historical perspective of the tool, along with its detailed classification. The next chapters describe bioinformatic tools in CRISPR/Cas, and delivery methods for CRISPR/Cas. The book also discusses about the applications of CRISPR/Cas beyond genome editing and use of CRISPR for rewriting genetic codes. The book dedicates a section to the use of CRISPR in plants. The book culminates with a chapter on the current status, challenges and shortcomings of the CRISPR/Cas genome editing tool. The book would be highly interesting to students and researchers in molecular biology, biochemistry, biotechnology, food science, agriculture and plant sciences.
Download or read book CRISPR Cas Systems written by Rodolphe Barrangou and published by Springer Science & Business Media. This book was released on 2012-12-13 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.
Download or read book Plant Genome Editing Technologies written by Jen-Tsung Chen and published by Springer Nature. This book was released on with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Engineering Disease Resistance in Plants using CRISPR Cas written by Zulqurnain Khan and published by CRC Press. This book was released on 2023-07-03 with total page 183 pages. Available in PDF, EPUB and Kindle. Book excerpt: CRISPR genome-editing technology presents opportunities to engineer disease resistance traits in plants and improve crop quality. Engineering Disease Resistance in Plants using CRISPR-Cas introduces readers to the basics of CRISPR-Cas and discusses its potential uses in various fields. The book focuses on methods of developing disease-resistant crops using CRISPR-Cas–mediated plant disease resistance modification. Comprehensively written, the author details all types and variants of the CRISPR toolkit. The book opens with information on the evolution of the CRISPR technology and follows a chronology of its development. Although the book concentrates on the use of CRISPR-Cas for disease resistance in plants, it also covers the technology's broader potential examining the history and development of other genome-editing tools. Key Features: Investigates the regulatory, ethical, and societal considerations while designing experiments. Discusses topics on disease development, control, and plant defense mechanisms. Examines genome-editing tools including Zinc Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs). Examines production technology to reduce bacterial, fungal, and viral diseases. Provides information for users to discover ways to overcome the challenges associated with food security. This book is a valuable resource for researchers, scientists, and undergraduate and graduate students who wish to gain a comprehensive understanding of genome-editing methods.
Download or read book Targeted Genome Editing Using Site Specific Nucleases written by Takashi Yamamoto and published by Springer. This book was released on 2015-01-05 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.
