Download or read book Avian Metapneumovirus Studies Using Reverse Genetics written by Marco Falchieri and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Avian metapneumovirus (AMPV) is an enveloped negative sense single stranded RNA virus which is a major endemic respiratory pathogen of global domestic poultry. Since reverse genetic (RG) techniques have been applied to this pathogen several reports have investigated the effects of single and multiple genomic mutations and gene deletions or insertions on viral biology. The aim of this study was to gain a better understanding of the viral capacity to accept and in some cases express homologous and heterologous extra sequences. Initially an AMPV subtype A was modified to introduce a homologous 200 bp sequence within the G gene and this recombinant was suggested to be used as a positive control for validating all stages of a previously established RT-nested PCR. Different Green Fluorescent Protein (GFP) AMPV recombinants were then prepared each one containing the reporter gene in a different intergenic position and then were assessed for expression, stability and viability. In particular, quantification of the expression was calculated using a quantitative ELISA. All recombinants showed high stability, while good viability was observed in all the positions, except when GFP was inserted between nucleocapsid (N) and phosphoprotein (P). The highest expression was detected in the virus with the insertion between N and P as expected, according to the transcriptional model for non-segmented negative stranded viruses. However GFP was produced at high levels even when inserted at the trailer end intergenic positions. Poor expression was seen for all the other positions. The vectoring abilities of subtype A strains were further investigated to accept and express foreign genes, specifically GFP gene and both spike (S1) and nucleocapsid (N) genes of infectious bronchitis virus (IBV). After viruses had been recovered by RG, all recombinants were proven to express the inserted genes efficiently and were all found to be highly stable during passage in vitro. Subsequently IBV recombinants were tested as candidate vaccines by eye-drop inoculation of one-day-old chickens. When chicks were challenged with IBV, partial protection results were observed, as assessed by greater motility of tracheal cilia from animals receiving the recombinants. Finally the development of a new RG system was attempted in order to extend this type of studies to the B subtype. This subtype is distributed worldwide and growing field evidence suggests it to be more able to infect commercial chickens compared to subtype A. For this reason it would be convenient to have an RG available also for B viruses. The construction of a DNA copy of the viral genome was attempted using site-directed mutagenesis and ligation techniques, resulting in more than 85% of the genome cloned. Unfortunately full genome cloning proved to be not possible, as severe problems were encountered, including construct instability and cloning bacteria intolerance to viral sequences.
Download or read book Reverse Genetics of Avian Metapneumovirus written by Govindarajan Dhanasekaran and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development and Use of Avian Pneumovirus Reverse Genetics Systems written by Nicole Lynn Edworthy and published by . This book was released on 2008 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Avian Virology written by Siba K. Samal and published by . This book was released on 2019-08-14 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: An invaluable reference source for everyone working on avian diseases. It is also highly recommended for all veterinary school and university libraries.
Download or read book Diseases of Poultry written by and published by John Wiley & Sons. This book was released on 2019-11-19 with total page 1488 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most complete and definitive reference to all aspects of poultry diseases, Diseases of Poultry, Fourteenth Edition has been fully revised and updated to offer a comprehensive survey of current knowledge. Updates the definitive reference of poultry health and disease Provides more clinically relevant information on management of specific diseases, contributed by clinical poultry veterinarians Offers information on disease control in organic and antibiotic-free production Presents more concise, streamlined chapters for ease of use Incorporates advances in the field, from new diagnostic tools and information to changes brought about by the increasing globalization and the re-emergence of zoonotic pathogens
Download or read book Vaccine Design written by Sunil Thomas and published by Humana. This book was released on 2016-04-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Chapters detail protocols for developing novel vaccines against infectious bacteria, viruses, fungi, and parasites for humans and animals. Volume 2: Vaccines for Veterinary Diseases includes vaccines for farm animals and fishes, vaccine vectors and production, vaccine delivery systems, vaccine bioinformatics, vaccine regulation and intellectual property. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Vaccine Design: Methods and Protocols, Volume 2: Vaccines for Veterinary Diseases aims to ensure successful results in the further study of this vital field.
Download or read book Nucleic Acids Advances in Research and Application 2013 Edition written by and published by ScholarlyEditions. This book was released on 2013-06-21 with total page 283 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nucleic Acids—Advances in Research and Application: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about RNA. The editors have built Nucleic Acids—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about RNA in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Nucleic Acids—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Download or read book Physiological Processes Advances in Research and Application 2012 Edition written by and published by ScholarlyEditions. This book was released on 2012-12-26 with total page 22 pages. Available in PDF, EPUB and Kindle. Book excerpt: Physiological Processes—Advances in Research and Application: 2012 Edition is a ScholarlyPaper™ that delivers timely, authoritative, and intensively focused information about Physiological Processes in a compact format. The editors have built Physiological Processes—Advances in Research and Application: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Physiological Processes in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Physiological Processes—Advances in Research and Application: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Download or read book Advances in Pneumovirinae Research and Application 2012 Edition written by and published by ScholarlyEditions. This book was released on 2012-12-26 with total page 34 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Pneumovirinae Research and Application / 2012 Edition is a ScholarlyPaper™ that delivers timely, authoritative, and intensively focused information about Pneumovirinae in a compact format. The editors have built Advances in Pneumovirinae Research and Application / 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Pneumovirinae in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Advances in Pneumovirinae Research and Application / 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Download or read book Pneumoviruses and Metapneumoviruses written by Helene F. Rosenberg and published by MDPI. This book was released on 2018-10-09 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Pneumoviruses and Metapneumoviruses" that was published in Viruses
Download or read book Avian Influenza Virus written by Erica Spackman and published by Springer Science & Business Media. This book was released on 2008-02-28 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the growing global fear of a major pandemic, avian influenza (AI) virus research has greatly increased in importance. In Avian Influenza Virus, an expert team of researchers and diagnosticians examine the fundamental, yet essential, virological methods for AI virus research and diagnostics as well as some of the newest molecular procedures currently used for basic and applied research. They present exciting, cutting-edge new methods that focus both on studying the virus itself and on work with avian hosts, an area greatly lacking in research.
Download or read book Emerging Viral Diseases written by Institute of Medicine and published by National Academies Press. This book was released on 2015-03-19 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past half century, deadly disease outbreaks caused by novel viruses of animal origin - Nipah virus in Malaysia, Hendra virus in Australia, Hantavirus in the United States, Ebola virus in Africa, along with HIV (human immunodeficiency virus), several influenza subtypes, and the SARS (sudden acute respiratory syndrome) and MERS (Middle East respiratory syndrome) coronaviruses - have underscored the urgency of understanding factors influencing viral disease emergence and spread. Emerging Viral Diseases is the summary of a public workshop hosted in March 2014 to examine factors driving the appearance, establishment, and spread of emerging, re-emerging and novel viral diseases; the global health and economic impacts of recently emerging and novel viral diseases in humans; and the scientific and policy approaches to improving domestic and international capacity to detect and respond to global outbreaks of infectious disease. This report is a record of the presentations and discussion of the event.
Download or read book Issues in Pharmacology Pharmacy Drug Research and Drug Innovation 2011 Edition written by and published by ScholarlyEditions. This book was released on 2012-01-09 with total page 6802 pages. Available in PDF, EPUB and Kindle. Book excerpt: Issues in Pharmacology, Pharmacy, Drug Research, and Drug Innovation: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Pharmacology, Pharmacy, Drug Research, and Drug Innovation. The editors have built Issues in Pharmacology, Pharmacy, Drug Research, and Drug Innovation: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Pharmacology, Pharmacy, Drug Research, and Drug Innovation in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Pharmacology, Pharmacy, Drug Research, and Drug Innovation: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Download or read book Avian Influenza and Newcastle Disease written by Illaria Capua and published by Springer Science & Business Media. This book was released on 2009-06-24 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Avian Influenza (AI) and Newcastle Disease (ND) are two devastating diseases of poultry, which cause losses to the poultry industry and influence the liveability of rural communities worldwide. Following the H5N1 epidemic they appear to be endemic at least in Asia, Eastern Europe, The Middle East and Africa. Particularly in case of AI outbreaks it is essential that infection is diagnosed promptly and that isolates are made available to the international scientific community. Currently, several organisations including OIE, FAO and the EC have organised training courses in affected areas. However, often these courses do not cover all aspects of AI/ND diagnosis but only certain aspects. This results in fragmented areas of knowledge and in the application of different diagnostic protocols in different parts of the world. The objective of this book is to provide a comprehensive approach to AI diagnosis ranging from the clinical elements that should trigger a suspicion in the field, to the post mortem technique, collection of samples, processing/ shipment of specimens, virological, serological and molecular diagnosis and guidelines for notification.
Download or read book Maternal Immunization written by Elke Leuridan and published by Academic Press. This book was released on 2019-11-26 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunization during pregnancy with currently recommended vaccines prevents infection in the mother, the unborn fetus, and the young infant, and there is an increasing focus from different stakeholders to use this approach for other infections of importance to protect these vulnerable groups. The aim of this Maternal Immunization book is to provide a contemporary overview of vaccines used in pregnancy (and the lactation period), with emphasis on aspects of importance for the target groups, namely, rationale for the use of vaccines in pregnancy, safety, immunogenicity (immunology), timing to vaccinate, repeat doses, protective effects in the mother, fetus, and infant, and public acceptance and implementation, of existing and of future vaccines. Provides an overview of a quickly evolving topic. This will benefit the reader who wishes to rapidly become informed and up-to-date with new developments in this field Suitable to a broad audience: scientific researchers, obstetricians, gynecologists, neonatologists, vaccinators, pediatricians, students, and industry. Maternal vaccination impacts a wide range of specialists Allows health care professionals/researchers to gain insight into other aspects of vaccination in pregnancy outside of their specialism Is coauthored by specialists from multiple disciplines, providing a diverse view of the subject, increasing its interest and appeal Creates awareness of the current developments in this area of medicine and of the potential of maternal vaccination to improve the health of mothers and infants worldwide
Download or read book Biology of Negative Strand RNA Viruses The Power of Reverse Genetics written by Yoshihiro Kawaoka and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: Negative-strand RNA viruses, so named because of the polarity of their genomic RNA to mRNA, include important human and non-human pathogens. This volume covers major advances in reverse genetics techniques over the past decade, state-of-the-art basic science and the clinical implications of experimental findings. This should rekindle interest in negative-strand RNA viruses among readers, including those in other disciplines, leading to further progress in understanding these important viruses and in developing effective measures of control.
Download or read book Viruses and Human Disease written by Ellen G. Strauss and published by Elsevier. This book was released on 2007-09-21 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: Completely revised and updated, the new edition of this groundbreaking text integrates basic virology with pathophysiological conditions to examine the connection between virology and human disease. Most virology textbooks focus on the molecular biology involved without adequate reference to physiology. This text focuses on viruses that infect humans, domestic animals and vertebrates and is based on extensive course notes from James Strauss’ virology class at the California Institute of Technology taught for over 30 years. Expertly depicting in color the molecular structure and replication of each virus, it provides an excellent overview for students and professionals interested in viruses as agents of human disease. Includes over 30% new material - virtually all of the figures and tables have been redrawn to include the latest information and the text has been extensively rewritten to include the most up-to-date information Includes a new chapter on emerging and reemerging viral diseases such as avian flu, SARS, the spread of West Nile virus across America, and the continuing spread of Nipah virus in Southeast Asia Further reading sections at the end of each chapter make it easy find key references World maps depicting the current distribution of existing and newly emerging viruses are also incorporated into the text
