Download or read book Automated Enzyme Reactions written by Heriberto Barrera-Benitez and published by . This book was released on 1968 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Automated Enzyme Assays written by Donald Bernard Roodyn and published by Elsevier. This book was released on 1970 with total page 225 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Colorimetric Determination of Nitrate Plus Nitrite in Water by Enzymatic Reduction Automated Discrete Analyzer Methods written by Charles J Patton and published by CreateSpace. This book was released on 2014-06-16 with total page 44 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report documents work at the U.S. Geological Sur- vey (USGS) National Water Quality Laboratory (NWQL) to validate enzymatic reduction, colorimetric determinative meth- ods for nitrate + nitrite in filtered water by automated discrete analysis. In these standard- and low-level methods (USGS I-2547-11 and I-2548-11), nitrate is reduced to nitrite with nontoxic, soluble nitrate reductase rather than toxic, granular, copperized cadmium used in the longstanding USGS auto- mated continuous-flow analyzer methods I-2545-90 (NWQL laboratory code 1975) and I-2546-91 (NWQL laboratory code 1979). Colorimetric reagents used to determine resulting nitrite in aforementioned enzymatic- and cadmium-reduction meth- ods are identical. The enzyme used in these discrete analyzer methods, designated AtNaR2 by its manufacturer, is produced by recombinant expression of the nitrate reductase gene from wall cress (Arabidopsis thaliana) in the yeast Pichia pastoris. Unlike other commercially available nitrate reductases we evaluated, AtNaR2 maintains high activity at 37°C and is not inhibited by high-phenolic-content humic acids at reaction temperatures in the range of 20°C to 37°C. These previously unrecognized AtNaR2 characteristics are essential for success- ful performance of discrete analyzer nitrate + nitrite assays (henceforth, DA-AtNaR2) described here.
Download or read book Practical Enzymology written by Hans Bisswanger and published by John Wiley & Sons. This book was released on 2019-11-06 with total page 574 pages. Available in PDF, EPUB and Kindle. Book excerpt: A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.
Download or read book Biomass Modification Characterization and Process Monitoring Analytics to Support Biofuel and Biomaterial Production written by Robert Henry and published by Frontiers Media SA. This book was released on 2016-06-09 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: The conversion of lignocellulosic biomass into renewable fuels and other commodities has provided an appealing alternative towards supplanting global dependence on fossil fuels. The suitability of multitudes of plants for deconstruction to useful precursor molecules and products is currently being evaluated. These studies have probed a variety of phenotypic traits, including cellulose, non-cellulosic polysaccharide, lignin, and lignin monomer composition, glucose and xylose production following enzymatic hydrolysis, and an assessment of lignin-carbohydrate and lignin-lignin linkages, to name a few. These quintessential traits can provide an assessment of biomass recalcitrance, enabling researchers to devise appropriate deconstruction strategies. Plants with high polysaccharide and lower lignin contents have been shown to breakdown to monomeric sugars more readily. Not all plants contain ideal proportions of the various cell wall constituents, however. The capabilities of biotechnology can alleviate this conundrum by tailoring the chemical composition of plants to be more favorable for conversion to sugars, fuels, etc. Increases in the total biomass yield, cellulose content, or conversion efficiency through, for example, a reduction in lignin content, are pathways being evaluated to genetically improve plants for use in manufacturing biofuels and bio-based chemicals. Although plants have been previously domesticated for food and fiber production, the collection of phenotypic traits prerequisite for biofuel production may necessitate new genetic breeding schemes. Given the plethora of potential plants available for exploration, rapid analytical methods are needed to more efficiently screen through the bulk of samples to hone in on which feedstocks contain the desired chemistry for subsequent conversion to valuable, renewable commodities. The standard methods for analyzing biomass and related intermediates and finished products are laborious, potentially toxic, and/or destructive. They may also necessitate a complex data analysis, significantly increasing the experimental time and add unwanted delays in process monitoring, where delays can incur in significant costs. Advances in thermochemical and spectroscopic techniques have enabled the screening of thousands of plants for different phenotypes, such as cell-wall cellulose, non-cellulosic polysaccharide, and lignin composition, lignin monomer composition, or monomeric sugar release. Some instrumental methods have been coupled with multivariate analysis, providing elegant chemometric predictive models enabling the accelerated identification of potential feedstocks. In addition to the use of high-throughput analytical methods for the characterization of feedstocks based on phenotypic metrics, rapid instrumental techniques have been developed for the real-time monitoring of diverse processes, such as the efficacy of a specific pretreatment strategy, or the formation of end products, such as biofuels and biomaterials. Real-time process monitoring techniques are needed for all stages of the feedstocks-to-biofuels conversion process in order to maximize efficiency and lower costs by monitoring and optimizing performance. These approaches allow researchers to adjust experimental conditions during, rather than at the conclusion, of a process, thereby decreasing overhead expenses. This Frontiers Research Topic explores options for the modification of biomass composition and the conversion of these feedstocks into to biofuels or biomaterials and the related innovations in methods for the analysis of the composition of plant biomass, and advances in assessing up- and downstream processes in real-time. Finally, a review of the computational models available for techno-economic modeling and lifecycle analysis will be presented.
Download or read book Enzyme Kinetics and Mechanism written by Paul F. Cook and published by Garland Science. This book was released on 2007-03-06 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.
Download or read book Devices and Systems for Laboratory Automation written by Kerstin Thurow and published by John Wiley & Sons. This book was released on 2022-08-03 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: Devices and Systems for Laboratory Automation Structured Overview on the Available Systems and Devices for Laboratory Automation Choosing the right systems and devices for the automation in any given laboratory is an essential part for the process to succeed. As relevant information to make an informed choice is not always readily available, a structured overview is essential for modern scientists. This book provides an introduction into laboratory automation and an overview of the necessary devices and systems. Sample topics discussed by the two well-qualified authors include: Specific requirements the automation needs to fulfill such as liquid delivery, low volume delivery, solid delivery, and sample preparation An overview on robots and mobile robots Common interfaces in laboratory automation For scientists and all individuals working in laboratories, the work serves as an indispensable resource in helping to make laboratory processes more streamlined, effective, and efficient.
Download or read book Enzyme Activity in Single Cells written by and published by Academic Press. This book was released on 2019-10-31 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme Activity in Single Cells, Volume 628, the latest release in the Methods of Enzymology series, discusses groundbreaking cellular physiology research that is taking place in the biological sciences. Chapters in this new release cover Spatial and temporal resolution of caspase waves in single Xenopus eggs during apoptosis, Spatial and temporal organization of metabolic complexes in cells, Measuring cellular efflux and biomolecular delivery: synthetic approaches to imaging and engineering cells, Slide-based, single-cell enzyme assays, Single-cell assays using integrated continuous-flow microfluidics, High-throughput screening of single-cell lysates, Microfluidic capture of single cells for drug resistance assays, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on Enzyme Activity in Single Cells
Download or read book Methods of Biochemical Analysis written by David Glick and published by John Wiley & Sons. This book was released on 2009-09-24 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemical analysis is a rapidly expanding field and is a key component of modern drug discovery and research. Methods of Biochemical Analysis provides a periodic and authoritative review of the latest achievements in biochemical analysis. Founded in 1954 by Professor David Glick, Methods of Biochemical Analysis provides a timely review of the latest developments in the field.
Download or read book Scientific Computing and Automation Europe 1990 written by E.J. Karjalainen and published by Elsevier. This book was released on 1990-12-17 with total page 517 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book comprises a large selection of papers presented at the second European Scientific Computing and Automation meeting (SCA 90 (Europe)) which was held in June 1990 in Maastricht, The Netherlands. The increasing use of computers for making measurements, interpreting data, and filing results brings a new unity to science. SCA concentrates on common computer-based tools which are useful in several disciplines. Practical problems in laboratory automation, robotics and information management with LIMS are covered in depth. The process of designing and acquiring a LIMS is described and standards for data transfer between instruments, between LIMS and instruments and between different LIMS are discussed. The applications of statistics and expert systems are covered in several chapters. Strategies for drug design are discussed with various practical examples. Finally the display of scientific results as images and computer-based animations is demonstrated by several examples with their color illustrations. The book should be of interest to those managing R&D projects, doing research in laboratories, acquiring or planning LIMS, designing instruments and laboratory automation systems and those involved in data analysis of scientific results.
Download or read book Enzyme Assays written by Robert Eisenthal and published by Practical Approach. This book was released on 2002 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used tomeasure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gelelectrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening;statistical analysis of enzyme kinetic data; and the determination of active site concentration.This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1977 with total page 904 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Industrial Enzymes and Their Applications written by Helmut Uhlig and published by John Wiley & Sons. This book was released on 1998-04-06 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: * Ein ma?gebender Katalog technischer Enzyme, ihrer Charakteristika und industriellen Verwendung. * Durch die leicht verstandliche Einfuhrung zu jedem Kapitel sowie die Beschreibung der technischen Enzyme ist dieses Werk auch fur Neulinge auf diesem Gebiet mit fehlendem wissenschaftlichen Hintergrund bestens geeignet. * Enthalten sind Informationen uber konkurrierende Enzymprodukte, so da? der Leser aufgrund dieses Wissens Enzyme fur bestimmte Zwecke auswahlen kann. * Sehr praxisorientiert, mit mehr als 100 Abbildungen, die genau zeigen, wie Enzymcharakteristika entwickelt werden. In jedem Kapitel finden sich zum besseren Verstandnis der technischen Seite Flie?diagramme. * Spezielle Kapitel fur die Leder, Back-, Saft- und Weinindustrie (Die wichtigsten Verbraucher industrieller Enzyme); einschlie?lich einem geschichtlichen Abri? uber die Verwendung von Enzymen in der Industrie * Au?erdem enthalten: Gesetzesvorschriften zum Einsatz industrieller Enzyme. (01/98)
Download or read book Principles of Enzyme Kinetics written by Athel Cornish-Bowden and published by Elsevier. This book was released on 2014-05-20 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles of Enzyme Kinetics discusses the principles of enzyme kinetics at an intermediate level. It is primarily written for first-year research students in enzyme kinetics. The book is composed of 10 chapters. Chapter 1 provides the basic principles of enzyme kinetics with a brief discussion of dimensional analysis. Subsequent chapters cover topics on the essential characteristics of steady-state kinetics, temperature dependence, methods for deriving steady-state rate equations, and control of enzyme activity. Integrated rate equations, and introductions to the study of fast reactions and the statistical aspects of enzyme kinetics are provided as well. Chemists and biochemists will find the book invaluable.
Download or read book Automation in Analytical Chemistry written by Leonard T. jr Skeggs and published by . This book was released on 1966 with total page 748 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Benzodiazepines and GHB written by Salvatore J. Salamone and published by Springer Science & Business Media. This book was released on 2001-09-14 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prominent experts explain the pharmacology and metabolism of benzodiazepines (LDB) and g-hydroxybutyric acid (GHB) and offer powerful and effective methodologies that will enable better detection of these drugs in various body fluids, as well as in hair. The techniques include highly sensitive immunoassay detection of LDBs, the detection of Rohypnol® and other LDBs by mass spectrometry, and the ultrasensitive detection of GHB. State-of-the-art and highly practical, Benzodiazepines and GHB: Detection and Pharmacology offers toxicologists, forensic scientists, and clinical chemists today's most effective methods for detecting these widely abused drugs that are sometimes found associated with criminal acts.
Download or read book Class 3 2 Hydrolases VII written by Dietmar Schomburg and published by Springer Science & Business Media. This book was released on 2003-06-18 with total page 660 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Springer Handbook of Enzymes provides concise data on some 5,000 enzymes sufficiently well characterized – and here is the second, updated edition. Their application in analytical, synthetic and biotechnology processes as well as in food industry, and for medicinal treatments is added. Data sheets are arranged in their EC-Number sequence. The new edition reflects considerable progress in enzymology: the total material has more than doubled, and the complete 2nd edition consists of 39 volumes plus Synonym Index. Starting in 2009, all newly classified enzymes are treated in Supplement Volumes.
