Download or read book Artificial Nucleases written by Marina A. Zenkova and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of agents capable of cleaving RNA and DNA has attracted considerable attention from researchers in the last few years, because of the immediate and very important applications they can find in the emerging fields of biotechnology and pharmacology. There are essentially two classes of these agents - nucleases that occur naturally inside cells and synthetically produced artificial nucleases. The first class includes protein enzyme nucle ases and catalytic RNA structured ribozymes that perform cleavage of the phosphodiester bonds in nucleic acids according to a hydrolytic pathway in the course of different biochemical processes in the cell. A different pathway is used by some antibiotics which cleave DNA via redox-based mechanisms resulting in oxidative damage of nucleotide units and breakage of the DNA backbone. The above molecules are indispensable tools for manipulating nucleic acids and processing RNA; DNA-cleaving antibiotics and cytotoxic ribonucleases have demonstrated utility as chemotherapeutic agents. The second class, artificial nucleases, are rationally designed to imitate the active centers of natural enzymes by simple structures possessing minimal sets of the most important characteristics that are essential for catalysis. A dif ferent approach, in vitro selection, was also used to create artificial RNA and DNA enzymes capable of cleaving RNA. Being less efficient and specific as compared to the natural enzymes, the primitive mimics are smaller and robust and can function in a broad range of conditions.
Download or read book Molecular Biology of Nucleases written by Nawin C. Mishra and published by CRC Press. This book was released on 1995-03-06 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nucleases occupy a central position in the biochemistry of DNA transactions and other metabolism of nucleic acids in all organisms. They have also proven useful in modern biological studies crucial for the development of recombinant DNA technology and reverse genetics. Nucleases assist in the identification and characterization of genes responsible for several diseases and their possible alleviation by gene therapy. Molecular Biology of Nucleases introduces the properties and biological roles of nucleases. It is the one comprehensive source for newcomers to the field.
Download or read book Artificial Enzymes written by Ronald Breslow and published by John Wiley & Sons. This book was released on 2006-05-12 with total page 193 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first book on this fascinating topic is edited by one of today's most famous and internationally respected organic chemists, renowned for his pioneering synthesis of the cyclopropenyl cation. For his part, Ronald Breslow has brought together leading scientists in this expanding area to provide a novel overview of protein-, cyclodextrin-, metal- and porphyrin-based artificial enzymes as well as enzyme-like polymers and dendrimers. A must for all scientists interested in this emerging field.
Download or read book Functional Genomics and Biotechnology in Solanaceae and Cucurbitaceae Crops written by Hiroshi Ezura and published by Springer. This book was released on 2016-01-04 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume summarizes recent technological advances in the design and engineering of Solanaceae and Cucurbitaceae crops. It begins with contributions on the tomato and melon genome sequence, databases for Solanaceae and Cucurbitaceae research, DNA markers in the breeding of the two families, and mutant resources and TILLING platforms in tomato research. Subsequent chapters address the use of molecular techniques for the modification of important breeding traits, such as tomato fruit set, growth, ripening, and sugar accumulation, as well as disease and insect resistance in melons. The volume closes with chapters on genome editing using artificial nucleases as a future breeding tool, and on the development of an in silico crop design system. It offers a valuable resource for plant breeders, molecular biologists, and agronomists.
Download or read book Nucleic Acids from A to Z written by Sabine M¿ller and published by John Wiley & Sons. This book was released on 2008-09-08 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concise but complete, this mini-encyclopedia contains over 1,500 entries covering all important concepts, compounds, techniques and acronyms for quick and easy reference. Guiding readers through the ever-increasing jungle of nucleic acid science and technology, the book distills the key information out of the large body of primary literature and presents it in a single volume. A first-stop resource for everyone, from students to established researchers, as both a desktop and library reference.
Download or read book Ribonucleases Part B Artificial and Engineered Ribonucleases and Speicifc Applications written by and published by Elsevier. This book was released on 2001-09-28 with total page 555 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second volume on ribonucleases provides up-to-date, methods-related information on these enzymes. Of particular interest to researchers will be the discussion of artificial and engineered ribonucleases, as well as the application of ribonucleases in medicine and biotechnology.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Download or read book Nucleic Acids Hybridization written by Anton Buzdin and published by Springer Science & Business Media. This book was released on 2007-06-07 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and biomedicine, while the potential of many other advantageous techniques seems to be underestimated. Written by an international team of authors, this book details the current state-of-art in hybridization techniques. Coverage includes experimental protocols along with comprehensive and detailed method descriptions.
Download or read book Library of Congress Subject Headings written by Library of Congress and published by . This book was released on 2007 with total page 2022 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Library of Congress Subject Headings written by Library of Congress. Cataloging Policy and Support Office and published by . This book was released on 2009 with total page 1924 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzymology Primer for Recombinant DNA Technology written by Hyone-Myong Eun and published by Elsevier. This book was released on 1996-06-03 with total page 727 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology Presents information in an easily accessible format to serve as a quick reference source Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques
Download or read book Handbook of Laboratory Animal Science written by Jann Hau and published by CRC Press. This book was released on 2021-05-17 with total page 1013 pages. Available in PDF, EPUB and Kindle. Book excerpt: Building upon the success of previous editions of the bestselling Handbook of Laboratory Animal Science, first published in 1994, this latest revision combines all three volumes in one definitive guide. It covers the essential principles and practices of Laboratory Animal Science as well as selected animal models in scientific disciplines where much progress has been made in recent years. Each individual chapter focuses on an important subdiscipline of laboratory animal science, and the chapters can be read and used as stand-alone texts, with only limited necessity to consult other chapters for information. With new contributors at the forefront of their fields, the book reflects the scientific and technological advances of the past decade. It also responds to advances in our understanding of animal behavior, emphasizing the importance of implementing the three Rs: replacing live animals with alternative methods, reducing the number of animals used, and refining techniques to minimize animal discomfort. This fourth edition will be useful all over the world as a textbook for laboratory animal science courses for postgraduate and undergraduate students and as a handbook for scientists who work with animals in their research, for university veterinarians, and for other specialists in laboratory animal science.
Download or read book DNA in Supramolecular Chemistry and Nanotechnology written by Eugen Stulz and published by John Wiley & Sons. This book was released on 2015-09-28 with total page 538 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the emerging topic of DNA nanotechnology and DNA supramolecular chemistry in its broader sense. By taking DNA out of its biological role, this biomolecule has become a very versatile building block in materials chemistry, supramolecular chemistry and bio-nanotechnology. Many novel structures have been realized in the past decade, which are now being used to create molecular machines, drug delivery systems, diagnosis platforms or potential electronic devices. The book combines many aspects of DNA nanotechnology, including formation of functional structures based on covalent and non-covalent systems, DNA origami, DNA based switches, DNA machines, and alternative structures and templates. This broad coverage is very appealing since it combines both the synthesis of modified DNA as well as designer concepts to successfully plan and make DNA nanostructures. Contributing authors have provided first a general introduction for the non-specialist reader, followed by a more in-depth analysis and presentation of their topic. In this way the book is attractive and useful for both the non-specialist who would like to have an overview of the topic, as well as the specialist reader who requires more information and inspiration to foster their own research.
Download or read book Development of Metal based Synthetic Nucleases and Peptidases written by Eric L. Hegg and published by . This book was released on 1996 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Human Genome Analysis Programme written by Manuel Hallen and published by IOS Press. This book was released on 1995 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first Human Genome Analysis Programme (HGAP) was launched for the years 1990-1992. The aim of this programme has been to ensure a significant European contribution to the worldwide effort to map the human genome and, in the long term, to set a basis for support of European research activities in future wide-ranging medical applications.
Download or read book Inorganic Chemical Biology written by Gilles Gasser and published by John Wiley & Sons. This book was released on 2014-06-23 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding, identifying and influencing the biological systems are the primary objectives of chemical biology. From this perspective, metal complexes have always been of great assistance to chemical biologists, for example, in structural identification and purification of essential biomolecules, for visualizing cellular organelles or to inhibit specific enzymes. This inorganic side of chemical biology, which continues to receive considerable attention, is referred to as inorganic chemical biology. Inorganic Chemical Biology: Principles, Techniques and Applications provides a comprehensive overview of the current and emerging role of metal complexes in chemical biology. Throughout all of the chapters there is a strong emphasis on fundamental theoretical chemistry and experiments that have been carried out in living cells or organisms. Outlooks for the future applications of metal complexes in chemical biology are also discussed. Topics covered include: • Metal complexes as tools for structural biology • IMAC, AAS, XRF and MS as detection techniques for metals in chemical biology • Cell and organism imaging and probing DNA using metal and metal carbonyl complexes • Detection of metal ions, anions and small molecules using metal complexes • Photo-release of metal ions in living cells • Metal complexes as enzyme inhibitors and catalysts in living cells Written by a team of international experts, Inorganic Chemical Biology: Principles, Techniques and Applications is a must-have for bioinorganic, bioorganometallic and medicinal chemists as well as chemical biologists working in both academia and industry.
Download or read book Chemistry and Biology of Artificial Nucleic Acids written by Martin Egli and published by Wiley-VCH. This book was released on 2012-04-16 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book to provide a comprehensive overview of the field of artificial nucleic acids. Covering a tremendous amount of literature on the chemistry, biology, and structure of artificial nucleic acids, it will constitute an invaluable source of information for the specialist and for young researchers interested in starting a career in this fascinating field of research alike. This book combines the contributions of many of the major players in this research field, and covers the synthesis of sugar-, base- and backbone-modified nucleic acids, their structural characteristics studied by X-ray crystallography, and NMR in solution as well as their chemical and biological properties.
Download or read book Small Molecule DNA and RNA Binders written by Martine Demeunynck and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 754 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of molecules that selectively bind to nucleic acids has provided many details about DNA and RNA recognition. The range of such substances, such as metal complexes, peptides, oligonucleotides and a wide array of synthetic organic compounds, is as manifold as the functions of nucleic acids. Nucleic acid recognition sequences are often found in the major or minor groove of a double strand, while other typical interactions include intercalation between base pairs or the formation of triple or quadruple helices. One example of a binding mode that has recently been proposed is end stacking on such complex structures as the telomere tetraplex. In this comprehensive book, internationally recognized experts describe in detail the important aspects of nucleic acid binding, and in so doing present impressive approaches to drug design. Since typical substances may be created naturally or synthetically, emphasis is placed on natural products, chemical synthesis, the use of combinatorial libraries, and structural characterization. The whole is rounded off by contributions on molecular modeling, as well as investigations into the way in which any given drug interacts with its nucleic acid recognition site.
