Download or read book Applications of Resonance Raman Spectroscopy to the Study of Bioinorganic Macromolecules written by Pearson Thomas Maugeri and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Metalloproteins are essential to life, with roles in cellular signaling, metabolism, gas cycles, and gene regulation, among others. One group of metalloproteins is the ferritin-like superfamily, which contains a wide variety of members ranging from iron-trafficking proteins (ferritin) to enzymes that can perform 2-electron chemistry on hydrocarbons (bacterial multicomponent monooxygenases or BMMs). Ribonucleotide reductases (RNRs), one of the members of the ferritin-like superfamily, are enzymes that catalyze the only known de novo reduction of ribonucleotides to deoxyribonucleotides and are found is nearly every organism on earth. Recently, a novel protein was discovered in Mycobacterium tuberculosis called R2lox, because of its sequence similarity to the R2 subunit of RNR, whose function remains unknown. However, it does not possess any RNR activity and is more similar in active site structure and proposed function to the BMMs. This protein is very important from a basic science standpoint as well as a health science standpoint because of its unique metal-binding capabilities and because it is upregulated in the virulent H37rv strain of M. tuberculosis. To study the active site structure and oxygen activation reaction of this protein, a custom resonance Raman spectroscopic system was designed and built. This system is very versatile in that it can access many different wavelengths with a single laser, which is useful for resonance Raman, which relies on tuning the Raman excitation beam to an electronic absorption to enhance the scattering efficiency of Raman light. In addition to R2lox, this custom resonance Raman system has been used on numerous bioinorganic molecules to great effect. This work describes the design and construction of the custom resonance Raman system and its use in studying R2lox and other bioinorganic systems. Additionally, experiments were performed to lay the groundwork for studying electron transfer in R2lox via ruthenium modification.
Download or read book Biochemical Applications of Raman and Resonance Raman Spectroscopes written by P Carey and published by Elsevier. This book was released on 2012-12-02 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemical Applications of Raman and Resonance Raman Spectroscopies focuses on the application of Raman and resonance Raman spectroscopies to biochemical problems. The book reviews biological systems and details the application of Raman spectroscopy to biological molecules such as proteins, nucleic acids, and lipids. It also looks at codevelopments of lasers, optics, and electronics that drive advances in experimental Raman spectroscopy, along with the important ramifications of these advances for biochemical applications. This volume is organized into eight chapters and begins with an overview of the theoretical and experimental aspects of Raman spectroscopy, including a very brief explanation of what Raman and resonance Raman spectroscopies are and a discussion of their advantages and disadvantages for biochemical studies. The explanation of the Raman and resonance Raman effects is taken up in more detail in the next chapter, which develops the concept of the vibrational motions of molecules by initially considering mechanical ""ball and spring"" models and goes on to use this concept to formulate a classical model for Raman scattering. The resonance Raman effect is then described by another model which emphasizes the discrete or quantized energy levels available to a molecule. The reader is also introduced to the experimental aspects of Raman spectroscopy and the application of Raman spectroscopy across the entire field of biochemistry. Each chapter contains an outline of the basic chemistry and biochemical nomenclature involved. This book will be of interest to chemists, biochemists, and spectroscopists, as well as graduate students and experienced research workers.
Download or read book Applications of Infrared Raman and Resonance Raman Spectroscopy in Biochemistry written by Frank S. Parker and published by Springer Science & Business Media. This book was released on 1983-10-01 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Raman Spectroscopy in Biology written by Anthony T. Tu and published by John Wiley & Sons. This book was released on 1982 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Spectroscopy of Biological Molecules written by Camille Sandorfy and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 639 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains the proceedings of the NATO-Advanced Study Institute on the "Spectroscopy of Biological Molecules", which took place on July 4-15, 1983 in Acquafredda di Maratea, Italy. The institute concentrated on three main subiects: the structure and dymanics of DNA, proteins, and visual and plant pigments. Its timeliness has been linked to rapid advances in certain spectroscopic techniques which yielded a consider able amount of new information on the structure and inter actions of biologically important molecules. Among these techniques Fourier transform infrared, resonance and surface enhanced 'Raman spectroscopies, Raman microscopy and micro probing, time resolved techniques, two photon and ultrafast electronic, and C-13, N-15 and P-31 NMR spectroscopies and kinetic and static IR difference spectroscopy receiced a great deal of attention at the Institute. In addition, an entirely new technique, near-millimeter-wave spectroscopy has been presented and discussed. Two introductory quantum chemical lectures, one on the structure of water in DNA, and another pn the energy bands in DNA and proteins set the stage for the experimentally oriented lectures that followed. Fundamental knowledge on hydrogen bonding was the topic of two other lectures. Panel discussions were held on the structure and confor mations of DNA, metal-DNA adducts and proteins and on visual pigments. Many scientists who normally attend different conferences and never meet, met at Aquafredda di Maratea. We feel, that at the end of our Institute a synthetic vi~w emerged on the powerful spectroscopic and theoretical methods which are now available for the study of biological molecules.
Download or read book Applications of Raman Spectroscopy to Biology written by M. Ghomi and published by IOS Press. This book was released on 2012-02-23 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Raman spectroscopy has been known and used as a technique for 80 years, originally for the study of inorganic substances. Recent advances in underlying technology, such as lasers, detectors, filters and components, have transformed the technique into a very effective modern tool for studying complex biological problems. Professor Mahmoud Ghomi (of the University of Paris XIII) has edited this book on the applications of Raman spectroscopy to biology, covering in a readily accessible way the area from basic studies to the diagnosis of disease. The early chapters provide background information on basic principles underlying the main Raman methods covered in the book, with information on Surface-Enhanced Raman Scattering (SERS) and Surface-Enhanced Fluorescence (SEF), as well as giving accounts of applications to biomolecular and cellular investigations. Among the topics covered are studies of drugs and their complexes with biomolecules on nanoparticles, application of SERS to blood analysis, studies of single cells and of applications to human cancer diagnostics.This will be a useful book for experimental scientists in academic, governmental, industrial and clinical environments and for those entering the field of biomolecular spectroscopy.
Download or read book Analytical Raman Spectroscopy written by Jeanette G. Grasselli and published by Wiley-Interscience. This book was released on 1991-09-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological Applications of Raman Spectroscopy Volume 1: Raman Spectra and the Conformations of Biological Macromolecules Edited by Thomas G. Spiro The first of the landmark volumes focusing on biological Raman applications, Volume 1 provides an incisive look at the current research surrounding the conformations of biological macromolecules. Volume 1 considers the varieties of macromolecules—proteins, nucleic acids, and lipids—in isolation and in the organized biological assemblies of ocular lenses, viruses, and membranes. The collective vibrational modes of macromolecules are considered theoretically, and there is a review of the promising area of vibrational optical activity. 1987 (0 471-81573-X) 352 pp. Volume 2: Resonance Raman Spectra of Polyenes and Aromatics The importance of the resonance Raman effect for biological applications is that it can provide dramatically increased sensitivity and selectivity The theory of the effect is presented in Volume 2, which also explores applications to rhodopsin, to the purine and pyrimidine bases of nucleic acids, to the peptide backbone and chromophoric side chains of proteins, to flavins, and, finally, to the introduction of resonance Raman labels into biological systems. 1987 (0 471-81574-8) 367 pp. Volume 3: Resonance Raman Spectra of Heme and Metalloproteins Heme proteins have provided an especially rich opportunity for Raman studies. Volume 3 describes in detail various aspects of work in this area, including applications to chlorophylls and nonheme metalloproteins. Coverage includes heme protein structure and the iron-histidine stretching mode; Raman scattering by cytochrome oxidase and by heme a model compounds; chlorophylls and the photosynthetic membrane; resonance Raman spectroscopy of blue copper proteins; and nonheme respiratory proteins. 1988 (0 471-81575-6) 565 pp.
Download or read book Infrared and Raman Spectra of Inorganic and Coordination Compounds Theory and Applications in Inorganic Chemistry written by Kazuo Nakamoto and published by Wiley-Interscience. This book was released on 1997-07-23 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most comprehensive guide to infrared and Raman spectra of inorganic and coordination compounds--now fully revised and updated Infrared and Raman Spectra of Inorganic and Coordination Compounds has always provided fundamental theories of vibrational spectroscopy in a condensed form and their applications to inorganic and coordination compounds. The Fifth Edition continues to cover these theories and applications, which have been updated by adding many new topics, figures, tables, and references. Part A of this two-volume work describes basic theories of normal vibrations including the method of normal coordinate analysis, resonance Raman spectroscopy, and vibrational analysis of crystals in clear and precise terms, and applies them to relatively simple inorganic compounds while leaving the applications to larger and more complex systems to Part B. This new edition * Incorporates new topics such as the correlation method, lattice vibrations, ceramic superconductors, and carbon clusters such as buckminsterfullerene * Offers numerous references to the recent research in the field * Reviews significant new literature on the subject * Provides many infrared and Raman spectral charts of typical compounds * Features 116 illustrations * Contains appendices consisting of tables, charts, and supplementary information Used independently or in combination with Part B, this is an excellent textbook for graduate-level course work, and the most comprehensive reference book available for researchers in the fields of vibrational spectroscopy, inorganic chemistry, coordination chemistry, organometallic chemistry, and bioinorganic chemistry. Also Available: Infrared and Raman Spectra of Inorganic and Coordination Compounds, 5th Edition, Part B: Applications in Coordination, Organometallic, and Bioinorganic Chemistry, 1997 0-471-16392-9
Download or read book Infrared and Raman Spectra of Inorganic and Coordination Compounds Part B written by Kazuo Nakamoto and published by John Wiley & Sons. This book was released on 2009-01-16 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 6th edition of this classic comprises the most comprehensive guide to infrared and Raman spectra of inorganic, organometallic, bioinorganic, and coordination compounds. From fundamental theories of vibrational spectroscopy to applications in a variety of compound types, it is extensively updated. Part B details applications of Raman and IR spectroscopy to larger and complex systems. It covers interactions of cisplatin and other metallodrugs with DNA and cytochrome c oxidase and peroxidase. This is a great reference for chemists and medical professionals working with infrared or Raman spectroscopies and for graduate students.
Download or read book Surface Enhanced Raman Spectroscopy written by Marek Prochazka and published by Springer. This book was released on 2015-12-12 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives an overview of recent developments in RS and SERS for sensing and biosensing considering also limitations, possibilities and prospects of this technique. Raman scattering (RS) is a widely used vibrational technique providing highly specific molecular spectral patterns. A severe limitation for the application of this spectroscopic technique lies in the low cross section of RS. Surface-enhanced Raman scattering (SERS) spectroscopy overcomes this problem by 6-11 orders of magnitude enhancement compared with the standard RS for molecules in the close vicinity of certain rough metal surfaces. Thus, SERS combines molecular fingerprint specificity with potential single-molecule sensitivity. Due to the recent development of new SERS-active substrates, labeling and derivatization chemistry as well as new instrumentations, SERS became a very promising tool for many varied applications, including bioanalytical studies and sensing. Both intrinsic and extrinsic SERS biosensing schemes have been employed to detect and identify small molecules, nucleic acids and proteins, and also for cellular and in vivo sensing.
Download or read book Vibrational Spectroscopy in Life Science written by Friedrich Siebert and published by John Wiley & Sons. This book was released on 2008-07-15 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: The authors describe basic theoretical concepts of vibrational spectroscopy, address instrumental aspects and experimental procedures, and discuss experimental and theoretical methods for interpreting vibrational spectra. It is shown how vibrational spectroscopy provides information on general aspects of proteins, such as structure, dynamics, and protein folding. In addition, the authors use selected examples to demonstrate the application of Raman and IR spectroscopy to specific biological systems, such as metalloproteins, and photoreceptors. Throughout, references to extensive mathematical and physical aspects, involved biochemical features, and aspects of molecular biology are set in boxes for easier reading. Ideal for undergraduate as well as graduate students of biology, biochemistry, chemistry, and physics looking for a compact introduction to this field.
Download or read book Raman Spectra and the Conformations of Biological Macromolecules written by Thomas G. Spiro and published by . This book was released on 1987 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomedical Vibrational Spectroscopy written by Peter Lasch and published by John Wiley & Sons. This book was released on 2008-06-27 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive overview of biomedical applications of vibrational spectroscopy focuses on methodologies that are most relevant to biodiagnostics. After a few introductory chapters that summarize the current status of the field, the reference covers current spectroscopic applications; new spectroscopic directions; and study design and the analysis of vibrational spectral fingerprints from complex biological and clinical samples . With chapters contributed by leading international experts, Biomedical Vibrational Spectroscopy is a core resource.
Download or read book Application of Computerized Raman Spectroscopy to the Study of Biological Macromolecules written by Rowena Lilian Sirey and published by . This book was released on 1978 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Raman Spectroscopy Volume I written by Günter G. Hoffmann and published by Momentum Press. This book was released on 2019-01-16 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book provides an up-to-date overview of the fast growing area of Raman spectroscopy. The two-volume work describes how analytic methods using Raman spectroscopy allow for the chemical analysis of materials, providing even spatial resolution without precedent. In addition, external perturbations (strain, temperature, pressure) on molecules and their alignment can be analyzed. Raman spectroscopy can also provide information about the interactions of components, again at a high level of spatial resolution. In the form of tip-enhanced Raman spectroscopy (TERS), the method is a valuable tool for nanotechnology. This book is intended for researchers or lecturers in chemistry and materials science, who are interested in the composition and properties of their samples. It describes how Raman spectroscopy will enable them to examine thin layers, surfaces, and interfaces and improve their knowledge about the properties of composites. In addition, it can serve as a short introduction to vibrational spectroscopy.
Download or read book Raman Spectroscopy written by Gustavo Morari Do Nascimento and published by BoD – Books on Demand. This book was released on 2018-04-18 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives a wide overview of the state-of-the-art applications of Raman spectroscopy in characterization of materials and biomaterials. The Raman signal is intrinsically smaller than other vibrational techniques; however, mainly through intensification processes, such as resonance Raman (RR) and surface-enhanced Raman spectroscopy (SERS), the Raman cross section can be strongly amplified. Thoroughly in these signal amplifications, the study of a diversity of chemical systems and the use of Raman technique for in situ and in vivo measurements is possible. The main goal of this book is to open up to an extended audience the possibilities of uses of Raman spectroscopy. In fact, this collective work will be beneficial to students, teachers, and researchers of many areas who are interested to expand their knowledge about Raman spectroscopy applied to nanotechnology, biotechnology, environmental science, inorganic chemistry, and health sciences.
Download or read book Spectroscopy of Biological Molecules Modern Trends written by P. Carmona and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 661 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 1997 European Conference on Spectroscopy of Biological Molecules (ECSBM) is the seventh in a biennial series of conferences devoted to the applications of molecular spectroscopy to biological molecules and related systems. The interest of these conferences rests mainly on the relationship between the structure and physiological activity of biological molecules and related systems of which these molecular species form part. This volume ofECSBM contains articles prepared by the invited lecturers and those making poster presentations at the seventh ECSBM. The reader will find mainly applications of vibrational spectroscopy to protein structure and dynamics, biomembranes, molecular recognition, nucleic acids and other biomolecules and biological systems containing specific chromophors. Biomedical applications of vibrational spectroscopy are expanding rapidly. On the other hand, a significant number of the papers describe applications of other methods, such as NMR, circular dichroism, optical absorption and fluorescence, X-ray absorption and diffraction and other theoretical methods. One aim has been to achieve a well balanced, critically comparative review of recent progress in the field of biomolecular structure, bonding and dynamics based on applications of the above spectroscopic methods. A great part of the contributions included in this volume are devoted to biomedical and biotechnological applications and provide a broadly based account of recent applicationS in this field. The content of this book has been organized in sections corresponding mainly to the different types of biological molecules investigated. This book includes also another section related to theoretical methods where MO calculations of vibrational frequencies dominate clearly the topic.
