Download or read book Apoptosis Methods and Protocols written by Hugh J. M. Brady and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most fundamental question facing each and every cell within an org- ism is to survive or to die. Cell death is required for normal function; some estimates suggest that as many as one million cells undergo cell death every second in the adult human body. Almost all cells undergoing physiological, or programmed, cell death, independent of cell type, manifest a stereotypic p- tern of morphological changes termed apoptosis. Typically, apoptotic cells d- play shrinkage, membrane blebbing, chromatin condensation, and nuclear fragmentation. The integrity of the cell membrane is not lost during apoptosis and so avoids eliciting the inflammatory response that would have been caused by the spillage of the cell’s contents. This is quite in contrast to the loss of cell contents typical of necrosis. The caspases, the family of intracellular cysteine proteases associated with apoptosis, are responsible for the stereotypical m- phological changes. Caspases cleave various substrate proteins that act on DNA fragmentation, nuclear envelope integrity, the cytoskeleton, and cell volume regulation. Apoptotic cells are cleared in vivo by the process of phagocytosis, in which specific “phagocytes” move to the site of apoptosis, engulf the dying cells and digest them. Apoptosis has a central role in many physiological processes, for example, in the immune system. Autoreactive cells are deleted via apoptosis to prevent autoimmunity. At the end of an immune response, activated lymphocytes are removed to maintain homeostasis within the immune system.
Download or read book Apoptosis written by P. Erhard and published by Humana Press. This book was released on 2009-07-15 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Apoptosis: Methods and Protocols, Second Edition constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death.
Download or read book Apoptosis and Cancer written by Gil Mor and published by Springer Science & Business Media. This book was released on 2007-10-11 with total page 271 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizing the mechanisms of apoptosis, as well as the industry laboratory, aimed at identifying new target molecules or screening for new compounds with potential clinical use.
Download or read book Programmed Cell Death Methods and Protocols written by Hamsa Puthalakath and published by Methods in Molecular Biology. This book was released on 2018-05-07 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Apoptosis written by P. Erhard and published by Humana Press. This book was released on 2009-07-15 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Apoptosis: Methods and Protocols, Second Edition constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death.
Download or read book Apoptosis Methods in Pharmacology and Toxicology written by Myrtle A. Davis and published by Springer Science & Business Media. This book was released on 2007-10-26 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dr. Myrtle A. Davis has assembled a panel of cutting-edge scientists to describe their best methods for detecting, illuminating, and quantifying apoptotic mechanisms in a way that is useful for the design of toxicology and pharmacology studies. These state-of-the-art techniques include flow cytometric, fluorometric, and laser scanning methods for quantifying and characterizing apoptosis, as well as protocols for the use of DNA microarray technology, high throughput screens, and ELISA. Immunocytochemical methods for measuring biochemical and molecular endpoints in tissue sections will be highly useful for those carrying out studies in whole animal models as opposed to cell culture systems.
Download or read book Apoptosis written by George Studzinski and published by OUP Oxford. This book was released on 1999-12-09 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text is designed to provide conceptual outlines and detailed procedures for basic and advanced studies of cell death by apoptosis. Chapters on the recognition of apoptosis as distinguished from necrosis and nonspecific cell DNA damage, are followed by a systematic examination of the established and the principal novel methodologies utilized by some leading laboratories conducting research on apoptosis. The organization is on the lines of signalling for apoptosis, the apoptotic cascade, and the execution of apoptosis. A wide variety of procedures are provided which will enable the reader to participate in cutting-edge research.
Download or read book Flow Cytometry Protocols written by Teresa S. Hawley and published by Springer Nature. This book was released on with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Apoptosis Detection and Assay Methods written by Li Zhu and published by Eaton Publishing Company/Biotechniques Books. This book was released on 1998 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Detection of Cell Death Mechanisms written by Ayesha B. Alvero and published by Humana. This book was released on 2021-12-29 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy and real-time multiplexed imaging assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Detection of Cell Death Mechanisms: Methods and Protocols aims to ensure seamless execution of protocols on specific cell death type.
Download or read book Programmed Necrosis written by Adrian T. Ting and published by Humana. This book was released on 2019-09-06 with total page 191 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the latest techniques and approaches used to study this emerging form of cell death. The chapters in this book cover topics such as distinguishing necroptosis from apoptosis; detecting MLKL oligomerization during programmed necrosis; analyzing the RIPK3 necrosome complex; using RIPK1 kinase small molecule inhibitors in studying necroptosis; monitoring RIPK1 phosphorylation in the TNFR1 signaling complex; and assessing in vivo kidney cell death in glomerular injury. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Programmed Necrosis: Methods and Protocols is a valuable resource for technicians, graduate students, postdoctoral fellows, and other scientists who are pursuing studies in this field.
Download or read book Apoptosis written by and published by Elsevier. This book was released on 2001-05-30 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: Apoptosis provides a current and comprehensive collection of methods for the study of cell death. Using a diverse range of technical approaches and model systems, the chapters in this volume cover topics from the cellular and organismal to the molecular and anatomical. The methods are illustrated with user-friendly recipes and over 100 tables, halftones, and diagrams. - Current methodologies for studying cell death - Wide range of model systems - Molecular, biochemical, cellular, and genetic approaches - Complements the original Cell Death volume - Up-to-date methodology for a fast moving field - Designed with the needs of both basic scientists and clinicians in mind - Authors are leaders in their respective fields
Download or read book Apoptosis Techniques and Protocols written by Andréa C. LeBlanc and published by Springer Science & Business Media. This book was released on 2008-02-06 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this revised and expanded second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis.
Download or read book Cancer Cell Culture written by Simon P. Langdon and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Methods to modify cancer cells are described in Part V, including protocols for transfection, development of drug-resistance, immortalization, and transfer in vivo. In Part VI methods of coculture of different cell types and contamination of cell lines are covered.
Download or read book Necrosis written by Kimberly McCall and published by Humana Press. This book was released on 2013-06-04 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell death is an essential process in development, and a major contributor to a wide range of human diseases. Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Necrosis: Methods and Protocols describes techniques in an easy to follow manner, with details so that beginners can succeed with challenging techniques.
Download or read book Plant Programmed Cell Death written by Laura De Gara and published by Humana Press. This book was released on 2018-01-14 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores numerous protocols that can be specifically used for studying plant programmed cell death (PCD), a mechanism involved in a number of physiological and pathological processes that are triggered by developmental requirements as well as changing/adverse environmental conditions. Rich in methodological recommendations, this book enables readers to perform the described analyses, alerting them to possible difficulties and showing how the methods can also be applied in different contexts and/or on different plant matrices. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Programmed Cell Death: Methods and Protocols provides essential methodological information that will help researchers to expand the field of plant PCD studies.
Download or read book Immune Homeostasis written by Andrew L. Snow and published by Humana Press. This book was released on 2013-02-09 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many questions remain unresolved as researchers continue to characterize and define the nature of normal immune homeostasis and determine how these processes are dysregulated in immunodeficiency, as well as in autoimmune and lymphoproliferative disorders. Immune Homeostasis: Methods and Protocols focuses on experimental techniques for measuring and analyzing immune cell dynamics, with a particular emphasis on examining lymphocyte programmed cell death in different contexts. With contributions by leading experts in the field, the collection examines detailed protocols for studying various pathways of apoptosis and necrosis in different types of hematopoietic cells, both in vitro and in vivo, methods for studying the maintenance of lymphocyte populations in the steady-state or following infectious challenges in both mice and humans, and technical insights into state-of-the-art genomics tools, among other topics. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Valuable and easy to use, Immune Homeostasis: Methods and Protocols serves as an important toolkit for basic and clinical scientists interested in examining various aspects of immune homeostasis in both normal and disease-related contexts in order to further study the dynamic processes that contribute to homeostasis of the immune system.
